Escherichia phage Ro45lw

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus Ro45lw

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9URS1|A0A3S9URS1_9CAUD Uncharacterized protein OS=Escherichia phage Ro45lw OX=2498616 GN=Ro45lw_04 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.1YY9 pKa = 10.68DD10 pKa = 4.19LLAATVEE17 pKa = 4.25LFNEE21 pKa = 5.17RR22 pKa = 11.84IQYY25 pKa = 10.6DD26 pKa = 4.53EE27 pKa = 4.08IAEE30 pKa = 4.24GDD32 pKa = 4.01DD33 pKa = 3.53YY34 pKa = 11.73HH35 pKa = 7.46DD36 pKa = 4.53ALHH39 pKa = 6.27EE40 pKa = 4.34VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.81IFTVMAADD60 pKa = 5.32GIDD63 pKa = 3.64NEE65 pKa = 4.91FEE67 pKa = 4.09DD68 pKa = 4.13SGYY71 pKa = 10.24IPEE74 pKa = 4.6TKK76 pKa = 10.15DD77 pKa = 2.93VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.09ALYY91 pKa = 10.84NDD93 pKa = 4.38VPNHH97 pKa = 6.31SDD99 pKa = 3.71VVWFEE104 pKa = 4.18AEE106 pKa = 4.11EE107 pKa = 3.95EE108 pKa = 4.25DD109 pKa = 5.48EE110 pKa = 4.23EE111 pKa = 4.84DD112 pKa = 4.0EE113 pKa = 4.55YY114 pKa = 11.2WVVDD118 pKa = 3.63AKK120 pKa = 10.47TGVFIEE126 pKa = 4.22QAVSLEE132 pKa = 4.14VATTCAKK139 pKa = 10.48DD140 pKa = 3.27HH141 pKa = 6.21YY142 pKa = 11.46ALGRR146 pKa = 11.84HH147 pKa = 5.98LKK149 pKa = 10.57VEE151 pKa = 5.07DD152 pKa = 3.67INDD155 pKa = 3.76TVVFDD160 pKa = 4.62PEE162 pKa = 4.2EE163 pKa = 4.29DD164 pKa = 3.89CEE166 pKa = 4.16

Molecular weight: 19.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9URS8|A0A3S9URS8_9CAUD DNA polymerase OS=Escherichia phage Ro45lw OX=2498616 GN=Ro45lw_21 PE=3 SV=1
MM1 pKa = 6.98VWALLVIGYY10 pKa = 9.14GLILAVITKK19 pKa = 10.28DD20 pKa = 2.71IAKK23 pKa = 9.83ARR25 pKa = 11.84KK26 pKa = 8.45VYY28 pKa = 9.99RR29 pKa = 11.84FQYY32 pKa = 10.09VSLGRR37 pKa = 11.84WTVRR41 pKa = 11.84QPNGRR46 pKa = 11.84FMRR49 pKa = 11.84NLANVWDD56 pKa = 4.08ISTLGSKK63 pKa = 10.1LL64 pKa = 3.47

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12012

41

1298

240.2

26.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.849 ± 0.391

0.999 ± 0.172

6.527 ± 0.258

6.693 ± 0.341

3.571 ± 0.199

8.009 ± 0.347

1.757 ± 0.196

4.712 ± 0.163

6.444 ± 0.345

7.992 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.118

4.321 ± 0.261

3.78 ± 0.202

3.929 ± 0.367

5.544 ± 0.236

6.285 ± 0.231

5.553 ± 0.254

7.484 ± 0.343

1.34 ± 0.146

3.488 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski