Amino acid dipepetide frequency for Escherichia phage Ro45lw

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.741AlaAla: 9.741 ± 1.108
1.249AlaCys: 1.249 ± 0.374
5.911AlaAsp: 5.911 ± 0.698
6.161AlaGlu: 6.161 ± 0.81
2.914AlaPhe: 2.914 ± 0.459
8.159AlaGly: 8.159 ± 0.931
0.833AlaHis: 0.833 ± 0.25
4.579AlaIle: 4.579 ± 0.506
6.411AlaLys: 6.411 ± 0.658
7.743AlaLeu: 7.743 ± 1.04
2.581AlaMet: 2.581 ± 0.452
3.58AlaAsn: 3.58 ± 0.49
2.414AlaPro: 2.414 ± 0.491
3.414AlaGln: 3.414 ± 0.532
3.58AlaArg: 3.58 ± 0.544
4.912AlaSer: 4.912 ± 0.573
3.247AlaThr: 3.247 ± 0.616
6.827AlaVal: 6.827 ± 0.865
1.249AlaTrp: 1.249 ± 0.439
2.997AlaTyr: 2.997 ± 0.514
0.0AlaXaa: 0.0 ± 0.0
Cys
0.416CysAla: 0.416 ± 0.167
0.083CysCys: 0.083 ± 0.085
0.916CysAsp: 0.916 ± 0.336
0.5CysGlu: 0.5 ± 0.206
0.666CysPhe: 0.666 ± 0.236
0.916CysGly: 0.916 ± 0.298
0.416CysHis: 0.416 ± 0.22
0.333CysIle: 0.333 ± 0.163
0.833CysLys: 0.833 ± 0.34
0.749CysLeu: 0.749 ± 0.26
0.25CysMet: 0.25 ± 0.209
0.5CysAsn: 0.5 ± 0.196
0.333CysPro: 0.333 ± 0.158
0.25CysGln: 0.25 ± 0.138
0.666CysArg: 0.666 ± 0.235
0.416CysSer: 0.416 ± 0.205
0.416CysThr: 0.416 ± 0.185
0.916CysVal: 0.916 ± 0.372
0.083CysTrp: 0.083 ± 0.085
0.333CysTyr: 0.333 ± 0.278
0.0CysXaa: 0.0 ± 0.0
Asp
6.494AspAla: 6.494 ± 0.699
0.749AspCys: 0.749 ± 0.339
4.246AspAsp: 4.246 ± 0.709
3.913AspGlu: 3.913 ± 0.489
2.081AspPhe: 2.081 ± 0.431
6.411AspGly: 6.411 ± 0.764
1.582AspHis: 1.582 ± 0.364
4.08AspIle: 4.08 ± 0.558
3.081AspLys: 3.081 ± 0.718
4.995AspLeu: 4.995 ± 0.666
2.081AspMet: 2.081 ± 0.393
2.331AspAsn: 2.331 ± 0.483
2.997AspPro: 2.997 ± 0.523
2.331AspGln: 2.331 ± 0.48
2.831AspArg: 2.831 ± 0.578
3.58AspSer: 3.58 ± 0.418
3.83AspThr: 3.83 ± 0.447
4.662AspVal: 4.662 ± 0.562
0.749AspTrp: 0.749 ± 0.271
2.248AspTyr: 2.248 ± 0.446
0.0AspXaa: 0.0 ± 0.0
Glu
7.077GluAla: 7.077 ± 1.071
0.666GluCys: 0.666 ± 0.252
4.746GluAsp: 4.746 ± 0.733
3.663GluGlu: 3.663 ± 0.588
2.414GluPhe: 2.414 ± 0.457
5.245GluGly: 5.245 ± 0.662
1.166GluHis: 1.166 ± 0.256
2.498GluIle: 2.498 ± 0.371
2.581GluLys: 2.581 ± 0.397
5.745GluLeu: 5.745 ± 0.747
1.915GluMet: 1.915 ± 0.42
2.414GluAsn: 2.414 ± 0.519
1.915GluPro: 1.915 ± 0.358
3.414GluGln: 3.414 ± 0.478
4.413GluArg: 4.413 ± 0.652
3.913GluSer: 3.913 ± 0.587
4.163GluThr: 4.163 ± 0.453
4.662GluVal: 4.662 ± 0.793
1.415GluTrp: 1.415 ± 0.337
2.914GluTyr: 2.914 ± 0.553
0.0GluXaa: 0.0 ± 0.0
Phe
3.081PheAla: 3.081 ± 0.569
0.333PheCys: 0.333 ± 0.189
2.581PheAsp: 2.581 ± 0.477
1.832PheGlu: 1.832 ± 0.365
1.166PhePhe: 1.166 ± 0.329
2.331PheGly: 2.331 ± 0.498
0.583PheHis: 0.583 ± 0.222
1.748PheIle: 1.748 ± 0.39
2.997PheLys: 2.997 ± 0.547
2.831PheLeu: 2.831 ± 0.408
0.666PheMet: 0.666 ± 0.234
2.248PheAsn: 2.248 ± 0.33
1.499PhePro: 1.499 ± 0.358
0.749PheGln: 0.749 ± 0.242
1.748PheArg: 1.748 ± 0.332
2.414PheSer: 2.414 ± 0.261
2.581PheThr: 2.581 ± 0.413
2.914PheVal: 2.914 ± 0.542
0.167PheTrp: 0.167 ± 0.119
1.249PheTyr: 1.249 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
6.827GlyAla: 6.827 ± 1.015
0.666GlyCys: 0.666 ± 0.276
4.912GlyAsp: 4.912 ± 0.761
5.245GlyGlu: 5.245 ± 0.607
2.498GlyPhe: 2.498 ± 0.472
5.245GlyGly: 5.245 ± 0.67
0.916GlyHis: 0.916 ± 0.306
4.163GlyIle: 4.163 ± 0.558
6.411GlyLys: 6.411 ± 0.739
6.91GlyLeu: 6.91 ± 0.798
2.831GlyMet: 2.831 ± 0.482
2.664GlyAsn: 2.664 ± 0.518
1.582GlyPro: 1.582 ± 0.377
3.164GlyGln: 3.164 ± 0.369
5.495GlyArg: 5.495 ± 0.471
5.495GlySer: 5.495 ± 0.568
4.246GlyThr: 4.246 ± 0.457
5.578GlyVal: 5.578 ± 0.694
1.082GlyTrp: 1.082 ± 0.315
4.163GlyTyr: 4.163 ± 0.626
0.0GlyXaa: 0.0 ± 0.0
His
0.749HisAla: 0.749 ± 0.306
0.333HisCys: 0.333 ± 0.182
1.249HisAsp: 1.249 ± 0.417
1.166HisGlu: 1.166 ± 0.334
0.5HisPhe: 0.5 ± 0.198
1.166HisGly: 1.166 ± 0.361
0.5HisHis: 0.5 ± 0.213
0.999HisIle: 0.999 ± 0.225
1.166HisLys: 1.166 ± 0.338
1.998HisLeu: 1.998 ± 0.412
0.583HisMet: 0.583 ± 0.184
0.666HisAsn: 0.666 ± 0.236
0.583HisPro: 0.583 ± 0.189
0.416HisGln: 0.416 ± 0.198
1.249HisArg: 1.249 ± 0.31
0.999HisSer: 0.999 ± 0.302
1.249HisThr: 1.249 ± 0.299
0.999HisVal: 0.999 ± 0.276
0.333HisTrp: 0.333 ± 0.192
0.666HisTyr: 0.666 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
3.414IleAla: 3.414 ± 0.567
0.666IleCys: 0.666 ± 0.268
2.997IleAsp: 2.997 ± 0.527
2.581IleGlu: 2.581 ± 0.452
1.166IlePhe: 1.166 ± 0.304
4.163IleGly: 4.163 ± 0.644
0.999IleHis: 0.999 ± 0.324
1.832IleIle: 1.832 ± 0.318
3.497IleLys: 3.497 ± 0.479
3.247IleLeu: 3.247 ± 0.491
0.999IleMet: 0.999 ± 0.382
2.664IleAsn: 2.664 ± 0.636
2.331IlePro: 2.331 ± 0.561
1.915IleGln: 1.915 ± 0.529
2.997IleArg: 2.997 ± 0.456
3.081IleSer: 3.081 ± 0.555
2.664IleThr: 2.664 ± 0.457
3.913IleVal: 3.913 ± 0.574
0.583IleTrp: 0.583 ± 0.213
1.415IleTyr: 1.415 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
7.743LysAla: 7.743 ± 0.984
0.666LysCys: 0.666 ± 0.226
3.913LysAsp: 3.913 ± 0.469
3.414LysGlu: 3.414 ± 0.39
2.165LysPhe: 2.165 ± 0.458
4.496LysGly: 4.496 ± 0.668
0.999LysHis: 0.999 ± 0.327
1.998LysIle: 1.998 ± 0.39
4.163LysLys: 4.163 ± 0.98
4.912LysLeu: 4.912 ± 0.778
2.081LysMet: 2.081 ± 0.437
2.498LysAsn: 2.498 ± 0.409
2.498LysPro: 2.498 ± 0.583
1.832LysGln: 1.832 ± 0.432
3.497LysArg: 3.497 ± 0.592
4.579LysSer: 4.579 ± 0.548
4.246LysThr: 4.246 ± 0.537
5.079LysVal: 5.079 ± 0.624
1.082LysTrp: 1.082 ± 0.313
2.581LysTyr: 2.581 ± 0.431
0.0LysXaa: 0.0 ± 0.0
Leu
7.41LeuAla: 7.41 ± 0.737
0.416LeuCys: 0.416 ± 0.168
4.579LeuAsp: 4.579 ± 0.452
6.661LeuGlu: 6.661 ± 0.699
2.581LeuPhe: 2.581 ± 0.365
4.995LeuGly: 4.995 ± 0.554
1.082LeuHis: 1.082 ± 0.229
3.164LeuIle: 3.164 ± 0.444
6.827LeuLys: 6.827 ± 0.742
5.079LeuLeu: 5.079 ± 0.598
2.165LeuMet: 2.165 ± 0.409
4.496LeuAsn: 4.496 ± 0.664
3.247LeuPro: 3.247 ± 0.408
3.83LeuGln: 3.83 ± 0.518
4.662LeuArg: 4.662 ± 0.473
5.661LeuSer: 5.661 ± 0.821
4.329LeuThr: 4.329 ± 0.663
5.745LeuVal: 5.745 ± 0.619
0.833LeuTrp: 0.833 ± 0.265
2.165LeuTyr: 2.165 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
2.997MetAla: 2.997 ± 0.401
0.416MetCys: 0.416 ± 0.169
1.249MetAsp: 1.249 ± 0.306
2.081MetGlu: 2.081 ± 0.393
0.749MetPhe: 0.749 ± 0.336
2.498MetGly: 2.498 ± 0.453
0.333MetHis: 0.333 ± 0.167
1.332MetIle: 1.332 ± 0.234
1.332MetLys: 1.332 ± 0.262
1.998MetLeu: 1.998 ± 0.385
0.583MetMet: 0.583 ± 0.179
1.082MetAsn: 1.082 ± 0.243
0.749MetPro: 0.749 ± 0.287
0.749MetGln: 0.749 ± 0.27
1.332MetArg: 1.332 ± 0.309
1.748MetSer: 1.748 ± 0.393
2.081MetThr: 2.081 ± 0.368
2.498MetVal: 2.498 ± 0.477
0.25MetTrp: 0.25 ± 0.189
1.166MetTyr: 1.166 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
4.163AsnAla: 4.163 ± 0.65
0.333AsnCys: 0.333 ± 0.176
2.248AsnAsp: 2.248 ± 0.413
2.747AsnGlu: 2.747 ± 0.527
1.748AsnPhe: 1.748 ± 0.267
4.413AsnGly: 4.413 ± 0.691
0.749AsnHis: 0.749 ± 0.241
1.998AsnIle: 1.998 ± 0.38
1.915AsnLys: 1.915 ± 0.325
3.414AsnLeu: 3.414 ± 0.605
1.415AsnMet: 1.415 ± 0.326
1.832AsnAsn: 1.832 ± 0.434
2.914AsnPro: 2.914 ± 0.583
1.748AsnGln: 1.748 ± 0.319
2.581AsnArg: 2.581 ± 0.553
2.498AsnSer: 2.498 ± 0.431
1.832AsnThr: 1.832 ± 0.44
2.997AsnVal: 2.997 ± 0.538
0.167AsnTrp: 0.167 ± 0.111
1.499AsnTyr: 1.499 ± 0.378
0.0AsnXaa: 0.0 ± 0.0
Pro
2.831ProAla: 2.831 ± 0.53
0.416ProCys: 0.416 ± 0.218
2.498ProAsp: 2.498 ± 0.318
3.414ProGlu: 3.414 ± 0.529
1.332ProPhe: 1.332 ± 0.316
1.665ProGly: 1.665 ± 0.311
0.749ProHis: 0.749 ± 0.251
1.832ProIle: 1.832 ± 0.366
2.914ProLys: 2.914 ± 0.652
2.331ProLeu: 2.331 ± 0.525
1.082ProMet: 1.082 ± 0.321
2.914ProAsn: 2.914 ± 0.394
0.833ProPro: 0.833 ± 0.331
1.582ProGln: 1.582 ± 0.473
1.582ProArg: 1.582 ± 0.356
2.165ProSer: 2.165 ± 0.299
2.498ProThr: 2.498 ± 0.445
3.414ProVal: 3.414 ± 0.495
0.749ProTrp: 0.749 ± 0.227
0.999ProTyr: 0.999 ± 0.231
0.0ProXaa: 0.0 ± 0.0
Gln
3.663GlnAla: 3.663 ± 0.555
0.25GlnCys: 0.25 ± 0.135
4.08GlnAsp: 4.08 ± 0.738
2.498GlnGlu: 2.498 ± 0.579
1.582GlnPhe: 1.582 ± 0.338
2.831GlnGly: 2.831 ± 0.422
0.666GlnHis: 0.666 ± 0.214
1.582GlnIle: 1.582 ± 0.384
1.748GlnLys: 1.748 ± 0.465
4.496GlnLeu: 4.496 ± 0.772
1.082GlnMet: 1.082 ± 0.343
1.832GlnAsn: 1.832 ± 0.525
1.166GlnPro: 1.166 ± 0.322
1.415GlnGln: 1.415 ± 0.494
2.581GlnArg: 2.581 ± 0.691
2.248GlnSer: 2.248 ± 0.363
1.832GlnThr: 1.832 ± 0.484
2.331GlnVal: 2.331 ± 0.457
0.416GlnTrp: 0.416 ± 0.175
0.916GlnTyr: 0.916 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
4.579ArgAla: 4.579 ± 0.692
0.666ArgCys: 0.666 ± 0.242
4.163ArgAsp: 4.163 ± 0.49
3.996ArgGlu: 3.996 ± 0.632
2.747ArgPhe: 2.747 ± 0.397
4.163ArgGly: 4.163 ± 0.429
0.749ArgHis: 0.749 ± 0.25
3.164ArgIle: 3.164 ± 0.656
3.913ArgLys: 3.913 ± 0.623
5.578ArgLeu: 5.578 ± 0.725
1.082ArgMet: 1.082 ± 0.379
2.414ArgAsn: 2.414 ± 0.461
1.582ArgPro: 1.582 ± 0.312
2.498ArgGln: 2.498 ± 0.419
2.581ArgArg: 2.581 ± 0.496
3.497ArgSer: 3.497 ± 0.561
2.331ArgThr: 2.331 ± 0.418
3.33ArgVal: 3.33 ± 0.59
0.916ArgTrp: 0.916 ± 0.272
1.499ArgTyr: 1.499 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
4.912SerAla: 4.912 ± 0.548
0.666SerCys: 0.666 ± 0.278
4.995SerAsp: 4.995 ± 0.418
3.081SerGlu: 3.081 ± 0.492
2.914SerPhe: 2.914 ± 0.402
5.745SerGly: 5.745 ± 0.921
2.414SerHis: 2.414 ± 0.46
2.914SerIle: 2.914 ± 0.536
3.414SerLys: 3.414 ± 0.504
3.913SerLeu: 3.913 ± 0.661
1.332SerMet: 1.332 ± 0.288
1.665SerAsn: 1.665 ± 0.343
2.747SerPro: 2.747 ± 0.503
2.248SerGln: 2.248 ± 0.478
3.747SerArg: 3.747 ± 0.542
4.413SerSer: 4.413 ± 0.665
3.747SerThr: 3.747 ± 0.727
4.579SerVal: 4.579 ± 0.554
0.666SerTrp: 0.666 ± 0.228
2.747SerTyr: 2.747 ± 0.526
0.0SerXaa: 0.0 ± 0.0
Thr
2.997ThrAla: 2.997 ± 0.653
0.416ThrCys: 0.416 ± 0.221
2.914ThrAsp: 2.914 ± 0.518
5.162ThrGlu: 5.162 ± 0.653
2.165ThrPhe: 2.165 ± 0.378
6.411ThrGly: 6.411 ± 0.808
0.416ThrHis: 0.416 ± 0.151
3.164ThrIle: 3.164 ± 0.496
3.663ThrLys: 3.663 ± 0.573
4.496ThrLeu: 4.496 ± 0.57
1.332ThrMet: 1.332 ± 0.254
1.499ThrAsn: 1.499 ± 0.451
3.33ThrPro: 3.33 ± 0.381
1.915ThrGln: 1.915 ± 0.488
2.581ThrArg: 2.581 ± 0.319
2.581ThrSer: 2.581 ± 0.506
2.997ThrThr: 2.997 ± 0.543
5.245ThrVal: 5.245 ± 0.912
0.749ThrTrp: 0.749 ± 0.216
1.499ThrTyr: 1.499 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
4.995ValAla: 4.995 ± 0.6
0.583ValCys: 0.583 ± 0.265
4.413ValAsp: 4.413 ± 0.701
6.161ValGlu: 6.161 ± 0.938
2.498ValPhe: 2.498 ± 0.492
5.328ValGly: 5.328 ± 0.539
1.332ValHis: 1.332 ± 0.54
3.663ValIle: 3.663 ± 0.553
4.829ValLys: 4.829 ± 0.527
5.661ValLeu: 5.661 ± 0.734
1.998ValMet: 1.998 ± 0.461
3.33ValAsn: 3.33 ± 0.558
3.33ValPro: 3.33 ± 0.538
3.414ValGln: 3.414 ± 0.561
4.163ValArg: 4.163 ± 0.565
5.245ValSer: 5.245 ± 0.594
4.579ValThr: 4.579 ± 0.693
5.745ValVal: 5.745 ± 1.087
1.082ValTrp: 1.082 ± 0.297
2.498ValTyr: 2.498 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
0.666TrpAla: 0.666 ± 0.233
0.083TrpCys: 0.083 ± 0.07
0.583TrpAsp: 0.583 ± 0.188
0.749TrpGlu: 0.749 ± 0.243
0.5TrpPhe: 0.5 ± 0.184
0.833TrpGly: 0.833 ± 0.283
0.333TrpHis: 0.333 ± 0.156
0.416TrpIle: 0.416 ± 0.268
1.166TrpLys: 1.166 ± 0.345
1.665TrpLeu: 1.665 ± 0.388
0.083TrpMet: 0.083 ± 0.075
0.916TrpAsn: 0.916 ± 0.255
0.167TrpPro: 0.167 ± 0.114
0.749TrpGln: 0.749 ± 0.303
0.833TrpArg: 0.833 ± 0.241
1.332TrpSer: 1.332 ± 0.458
0.416TrpThr: 0.416 ± 0.17
1.415TrpVal: 1.415 ± 0.351
0.167TrpTrp: 0.167 ± 0.113
0.333TrpTyr: 0.333 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.747TyrAla: 3.747 ± 0.58
0.333TyrCys: 0.333 ± 0.17
2.081TyrAsp: 2.081 ± 0.427
1.915TyrGlu: 1.915 ± 0.41
1.332TyrPhe: 1.332 ± 0.346
3.081TyrGly: 3.081 ± 0.455
0.749TyrHis: 0.749 ± 0.273
1.665TyrIle: 1.665 ± 0.474
1.582TyrLys: 1.582 ± 0.341
2.165TyrLeu: 2.165 ± 0.474
0.999TyrMet: 0.999 ± 0.268
1.665TyrAsn: 1.665 ± 0.315
1.582TyrPro: 1.582 ± 0.386
1.665TyrGln: 1.665 ± 0.572
2.331TyrArg: 2.331 ± 0.501
2.081TyrSer: 2.081 ± 0.468
2.248TyrThr: 2.248 ± 0.535
1.998TyrVal: 1.998 ± 0.418
0.666TyrTrp: 0.666 ± 0.267
0.999TyrTyr: 0.999 ± 0.281
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski