Rodent Torque teno virus 3
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4QBE6|A0A2H4QBE6_9VIRU Uncharacterized protein OS=Rodent Torque teno virus 3 OX=2054610 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.02 FSAAAKK8 pKa = 9.5 ILLTIFLDD16 pKa = 4.68 GVLLPALGRR25 pKa = 11.84 PSPLWLQEE33 pKa = 3.99 LEE35 pKa = 4.34 KK36 pKa = 10.54 ATVVPEE42 pKa = 4.03 TVEE45 pKa = 4.31 NKK47 pKa = 7.29 TTPLPGKK54 pKa = 7.35 EE55 pKa = 4.06 TPQAVVEE62 pKa = 4.55 TEE64 pKa = 3.95 NQACTCHH71 pKa = 7.32 AMEE74 pKa = 5.76 PDD76 pKa = 3.45 QQKK79 pKa = 10.54 EE80 pKa = 4.45 MYY82 pKa = 9.61 HH83 pKa = 6.36 SWILSFSILLGWPEE97 pKa = 3.97 GDD99 pKa = 3.99 NGFHH103 pKa = 7.48 LPRR106 pKa = 11.84 EE107 pKa = 4.36 QADD110 pKa = 2.69 SWMARR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 CSQQPSVSPRR127 pKa = 11.84 PVLGGEE133 pKa = 4.2 VLEE136 pKa = 4.63 STVEE140 pKa = 4.04 QQQSRR145 pKa = 11.84 LL146 pKa = 3.56
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.157
IPC2_protein 5.219
IPC_protein 5.092
Toseland 5.054
ProMoST 5.207
Dawson 5.067
Bjellqvist 5.194
Wikipedia 4.902
Rodwell 5.003
Grimsley 4.991
Solomon 5.067
Lehninger 5.016
Nozaki 5.194
DTASelect 5.27
Thurlkill 5.054
EMBOSS 4.952
Sillero 5.283
Patrickios 4.24
IPC_peptide 5.08
IPC2_peptide 5.283
IPC2.peptide.svr19 5.271
Protein with the highest isoelectric point:
>tr|A0A2H4QBE5|A0A2H4QBE5_9VIRU Uncharacterized protein OS=Rodent Torque teno virus 3 OX=2054610 PE=4 SV=1
MM1 pKa = 7.58 AFYY4 pKa = 10.63 SRR6 pKa = 11.84 RR7 pKa = 11.84 WGGLRR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 9.84 GYY16 pKa = 10.04 RR17 pKa = 11.84 NWRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 PWYY25 pKa = 8.56 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 WRR30 pKa = 11.84 TRR32 pKa = 11.84 PRR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 8.76 RR37 pKa = 11.84 VRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 LLRR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 7.1 TRR51 pKa = 11.84 HH52 pKa = 5.01 VPVMQWNPINKK63 pKa = 8.79 KK64 pKa = 9.04 RR65 pKa = 11.84 CTIRR69 pKa = 11.84 GFCPLVYY76 pKa = 10.47 CLGGQKK82 pKa = 10.81 GIMDD86 pKa = 3.89 FTYY89 pKa = 10.34 PGNKK93 pKa = 9.73 LILGWLGGGVHH104 pKa = 6.77 SSLLSLLDD112 pKa = 3.87 LFWEE116 pKa = 4.1 EE117 pKa = 4.41 RR118 pKa = 11.84 YY119 pKa = 8.89 WRR121 pKa = 11.84 ARR123 pKa = 11.84 WSSSNQGYY131 pKa = 9.16 NLFRR135 pKa = 11.84 YY136 pKa = 9.44 YY137 pKa = 11.08 GATITLYY144 pKa = 9.2 PVMYY148 pKa = 8.1 YY149 pKa = 9.9 TYY151 pKa = 9.61 IFWYY155 pKa = 8.2 STEE158 pKa = 3.97 EE159 pKa = 3.8 LTEE162 pKa = 4.16 DD163 pKa = 3.79 VEE165 pKa = 4.56 PLTVCHH171 pKa = 6.78 PSQLLLSKK179 pKa = 10.23 HH180 pKa = 5.53 HH181 pKa = 6.56 VIVHH185 pKa = 6.5 RR186 pKa = 11.84 LDD188 pKa = 4.4 GFQKK192 pKa = 8.88 TKK194 pKa = 10.21 PIKK197 pKa = 10.47 LHH199 pKa = 6.28 IKK201 pKa = 9.84 PPSQMTGTWHH211 pKa = 6.94 TFAAWAKK218 pKa = 10.07 QPLLKK223 pKa = 10.06 WRR225 pKa = 11.84 ISLLDD230 pKa = 3.97 IEE232 pKa = 5.05 NPWTGFPNDD241 pKa = 3.41 QTQGVHH247 pKa = 4.23 CTVWARR253 pKa = 11.84 HH254 pKa = 4.04 KK255 pKa = 9.27 TEE257 pKa = 4.4 RR258 pKa = 11.84 PTEE261 pKa = 4.0 ARR263 pKa = 11.84 SLDD266 pKa = 3.37 LWYY269 pKa = 10.56 FPLLDD274 pKa = 5.34 DD275 pKa = 4.6 GTDD278 pKa = 3.64 LSVTMHH284 pKa = 6.9 KK285 pKa = 10.39 IKK287 pKa = 10.14 WNNDD291 pKa = 2.6 GTGPKK296 pKa = 9.73 YY297 pKa = 10.77 DD298 pKa = 4.37 EE299 pKa = 4.69 ANFWPVAAQFQRR311 pKa = 11.84 LLVPFYY317 pKa = 9.61 MYY319 pKa = 11.21 GFGRR323 pKa = 11.84 SATFYY328 pKa = 11.52 DD329 pKa = 4.12 MNEE332 pKa = 4.29 TTHH335 pKa = 6.57 EE336 pKa = 4.48 PGPTEE341 pKa = 4.06 GNMGSFLFLKK351 pKa = 10.12 FLNPPCWRR359 pKa = 11.84 GSEE362 pKa = 4.25 GQFPQFKK369 pKa = 10.66 DD370 pKa = 3.64 DD371 pKa = 3.61 MCFMTFGTVAQLCAQGPWCMKK392 pKa = 10.46 RR393 pKa = 11.84 IGTNTAGVNISMKK406 pKa = 10.38 YY407 pKa = 10.19 KK408 pKa = 10.59 FYY410 pKa = 10.14 FQWGGTPGTNLPPVQPVAGNPGQPLLQSTLRR441 pKa = 11.84 WGNTIRR447 pKa = 11.84 ADD449 pKa = 3.16 IRR451 pKa = 11.84 DD452 pKa = 3.86 PTTVGEE458 pKa = 4.12 EE459 pKa = 4.28 VLWPEE464 pKa = 4.96 DD465 pKa = 3.83 LDD467 pKa = 3.48 EE468 pKa = 6.09 HH469 pKa = 7.45 GIITSQAFRR478 pKa = 11.84 RR479 pKa = 11.84 ITQPSVSTGSRR490 pKa = 11.84 SLGTLGCLRR499 pKa = 11.84 PAVSSGRR506 pKa = 11.84 KK507 pKa = 8.68 RR508 pKa = 11.84 PRR510 pKa = 11.84 HH511 pKa = 4.95 SFEE514 pKa = 3.77 EE515 pKa = 4.51 SEE517 pKa = 4.4 EE518 pKa = 4.32 EE519 pKa = 4.08 EE520 pKa = 4.62 HH521 pKa = 7.49 SSSPQEE527 pKa = 3.91 TEE529 pKa = 3.79 NYY531 pKa = 8.14 STEE534 pKa = 4.11 EE535 pKa = 4.02 EE536 pKa = 4.24 EE537 pKa = 5.04 EE538 pKa = 3.96 EE539 pKa = 4.02 MEE541 pKa = 3.96 RR542 pKa = 11.84 ARR544 pKa = 11.84 RR545 pKa = 11.84 KK546 pKa = 5.89 QHH548 pKa = 4.17 RR549 pKa = 11.84 RR550 pKa = 11.84 KK551 pKa = 10.0 RR552 pKa = 11.84 KK553 pKa = 9.48 RR554 pKa = 11.84 LEE556 pKa = 3.47 QLLRR560 pKa = 11.84 GLSNLGFRR568 pKa = 11.84 RR569 pKa = 11.84 PMMAGPSGNYY579 pKa = 8.88 QKK581 pKa = 11.25 SNSII585 pKa = 3.71
Molecular weight: 68.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.906
IPC2_protein 9.37
IPC_protein 9.633
Toseland 9.867
ProMoST 9.706
Dawson 10.145
Bjellqvist 9.897
Wikipedia 10.365
Rodwell 10.35
Grimsley 10.248
Solomon 10.175
Lehninger 10.131
Nozaki 9.955
DTASelect 9.882
Thurlkill 9.984
EMBOSS 10.306
Sillero 10.087
Patrickios 5.614
IPC_peptide 10.175
IPC2_peptide 8.858
IPC2.peptide.svr19 8.363
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
871
140
585
290.3
33.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.396 ± 1.922
1.493 ± 0.405
3.674 ± 0.741
6.774 ± 1.15
4.133 ± 0.573
7.577 ± 0.805
2.411 ± 0.339
3.444 ± 0.289
4.822 ± 0.621
9.07 ± 0.886
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.293
3.33 ± 0.647
7.348 ± 0.623
4.937 ± 0.945
8.955 ± 1.628
7.003 ± 0.919
6.544 ± 0.689
3.789 ± 1.274
3.559 ± 0.404
3.215 ± 1.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here