Streptococcus satellite phage Javan298
Average proteome isoelectric point is 5.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIT3|A0A4D5ZIT3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan298 OX=2558617 GN=JavanS298_0008 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.3 IKK4 pKa = 10.74 LFYY7 pKa = 10.44 QKK9 pKa = 10.61 YY10 pKa = 8.31 KK11 pKa = 10.71 QSLEE15 pKa = 4.1 DD16 pKa = 4.85 FEE18 pKa = 5.91 SQVNDD23 pKa = 3.44 FMATVEE29 pKa = 4.3 VVDD32 pKa = 3.98 VKK34 pKa = 11.25 YY35 pKa = 11.23 SEE37 pKa = 4.27 ATVGNSDD44 pKa = 5.52 DD45 pKa = 4.23 MDD47 pKa = 4.02 TLTSVMVLYY56 pKa = 10.56 KK57 pKa = 10.78
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.455
IPC2_protein 4.469
IPC_protein 4.279
Toseland 4.101
ProMoST 4.431
Dawson 4.253
Bjellqvist 4.418
Wikipedia 4.19
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.19
Sillero 4.406
Patrickios 3.77
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.347
Protein with the highest isoelectric point:
>tr|A0A4D5ZKM3|A0A4D5ZKM3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan298 OX=2558617 GN=JavanS298_0014 PE=4 SV=1
MM1 pKa = 7.86 LITSTQAKK9 pKa = 9.74 AIRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.66 QADD17 pKa = 3.4 KK18 pKa = 11.27 KK19 pKa = 10.16 LTAKK23 pKa = 10.18 QAGEE27 pKa = 4.41 EE28 pKa = 3.96 IGVTQVTYY36 pKa = 10.88 RR37 pKa = 11.84 KK38 pKa = 9.3 IRR40 pKa = 11.84 DD41 pKa = 3.51 GGEE44 pKa = 4.09 VKK46 pKa = 10.52 PSIYY50 pKa = 9.91 QKK52 pKa = 11.14 AMQWLAEE59 pKa = 4.48 DD60 pKa = 4.07 YY61 pKa = 11.23
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.984
IPC2_protein 9.18
IPC_protein 9.136
Toseland 9.955
ProMoST 9.619
Dawson 10.131
Bjellqvist 9.75
Wikipedia 10.277
Rodwell 10.687
Grimsley 10.189
Solomon 10.175
Lehninger 10.145
Nozaki 9.911
DTASelect 9.75
Thurlkill 9.984
EMBOSS 10.35
Sillero 10.028
Patrickios 10.452
IPC_peptide 10.175
IPC2_peptide 8.185
IPC2.peptide.svr19 8.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2554
39
508
141.9
16.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.756 ± 0.398
0.352 ± 0.115
6.578 ± 0.472
9.514 ± 1.048
4.307 ± 0.495
4.581 ± 0.312
1.527 ± 0.33
6.578 ± 0.452
9.593 ± 0.604
9.984 ± 0.454
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.423
5.795 ± 0.628
1.84 ± 0.21
4.464 ± 0.329
4.777 ± 0.239
4.973 ± 0.335
5.247 ± 0.646
5.521 ± 0.499
1.057 ± 0.178
4.816 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here