Rhodococcus phage Whack
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A515MKG8|A0A515MKG8_9CAUD Uncharacterized protein OS=Rhodococcus phage Whack OX=2591132 GN=66 PE=4 SV=1
MM1 pKa = 7.74 TYY3 pKa = 10.05 QHH5 pKa = 7.28 LDD7 pKa = 3.25 LGVDD11 pKa = 3.95 PFSEE15 pKa = 4.53 EE16 pKa = 4.57 CLPADD21 pKa = 3.95 PDD23 pKa = 4.04 PFHH26 pKa = 6.87 EE27 pKa = 5.2 HH28 pKa = 7.44 DD29 pKa = 4.62 PRR31 pKa = 11.84 DD32 pKa = 4.49 DD33 pKa = 3.71 APEE36 pKa = 3.86 WTKK39 pKa = 11.52
Molecular weight: 4.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.092
IPC2_protein 4.088
IPC_protein 3.973
Toseland 3.783
ProMoST 4.164
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.935
Sillero 4.113
Patrickios 3.16
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.01
Protein with the highest isoelectric point:
>tr|A0A515MKA3|A0A515MKA3_9CAUD Uncharacterized protein OS=Rhodococcus phage Whack OX=2591132 GN=42 PE=4 SV=1
MM1 pKa = 7.33 TNVLNVADD9 pKa = 3.63 NVYY12 pKa = 10.42 RR13 pKa = 11.84 NGRR16 pKa = 11.84 SGVKK20 pKa = 9.37 QAMTTDD26 pKa = 5.44 PISMHH31 pKa = 6.58 LGNLRR36 pKa = 11.84 LHH38 pKa = 6.46 NSSATTIKK46 pKa = 10.46 HH47 pKa = 6.29 RR48 pKa = 11.84 LDD50 pKa = 3.13 NLQRR54 pKa = 11.84 IQAKK58 pKa = 9.77 LGKK61 pKa = 9.02 PLIEE65 pKa = 4.31 ATADD69 pKa = 5.21 DD70 pKa = 4.16 LAAWQANLRR79 pKa = 11.84 ISPSSIATYY88 pKa = 8.11 TAHH91 pKa = 6.26 VRR93 pKa = 11.84 AFYY96 pKa = 10.45 RR97 pKa = 11.84 WAQLNEE103 pKa = 5.07 LITVDD108 pKa = 3.47 PTVFLVTPKK117 pKa = 10.46 LKK119 pKa = 10.41 RR120 pKa = 11.84 RR121 pKa = 11.84 MPRR124 pKa = 11.84 PIPEE128 pKa = 4.73 RR129 pKa = 11.84 DD130 pKa = 3.14 LSAALTTSRR139 pKa = 11.84 HH140 pKa = 4.7 DD141 pKa = 3.51 HH142 pKa = 5.77 QLFCAFLLAGYY153 pKa = 9.95 CGLRR157 pKa = 11.84 CGEE160 pKa = 3.97 ITRR163 pKa = 11.84 VTRR166 pKa = 11.84 SDD168 pKa = 3.19 VRR170 pKa = 11.84 EE171 pKa = 4.21 DD172 pKa = 3.63 EE173 pKa = 4.32 NGKK176 pKa = 9.38 GAYY179 pKa = 9.64 LVVHH183 pKa = 6.44 GKK185 pKa = 10.15 GGHH188 pKa = 4.95 EE189 pKa = 4.2 RR190 pKa = 11.84 IIRR193 pKa = 11.84 IAPDD197 pKa = 3.27 IYY199 pKa = 11.39 AEE201 pKa = 4.17 IRR203 pKa = 11.84 SLMYY207 pKa = 9.38 RR208 pKa = 11.84 TGPLFVSKK216 pKa = 10.45 SGNAYY221 pKa = 8.33 TPNRR225 pKa = 11.84 LTQITSAHH233 pKa = 6.23 LKK235 pKa = 10.69 AIGLPYY241 pKa = 9.61 TLHH244 pKa = 6.03 TLRR247 pKa = 11.84 HH248 pKa = 5.65 RR249 pKa = 11.84 FATRR253 pKa = 11.84 LADD256 pKa = 4.0 LGADD260 pKa = 3.47 VRR262 pKa = 11.84 DD263 pKa = 3.71 VQAALGHH270 pKa = 6.12 AHH272 pKa = 7.81 LSTTTLYY279 pKa = 10.75 LASNSRR285 pKa = 11.84 RR286 pKa = 11.84 STSSVDD292 pKa = 2.99 RR293 pKa = 11.84 LGRR296 pKa = 11.84 GLAAMAKK303 pKa = 8.46 RR304 pKa = 11.84 QPSARR309 pKa = 11.84 STSRR313 pKa = 11.84 DD314 pKa = 3.26 DD315 pKa = 4.67 DD316 pKa = 4.0 LL317 pKa = 6.92
Molecular weight: 35.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.604
IPC_protein 10.365
Toseland 10.35
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.262
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.599
Solomon 10.599
Lehninger 10.555
Nozaki 10.35
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.452
Patrickios 10.292
IPC_peptide 10.599
IPC2_peptide 9.253
IPC2.peptide.svr19 8.602
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
15671
38
1966
203.5
22.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.41 ± 0.5
0.753 ± 0.12
6.911 ± 0.289
6.12 ± 0.339
2.699 ± 0.166
8.181 ± 0.505
1.997 ± 0.174
4.977 ± 0.217
3.95 ± 0.229
7.913 ± 0.239
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.122
3.152 ± 0.188
5.89 ± 0.278
3.305 ± 0.142
6.63 ± 0.301
6.056 ± 0.209
6.649 ± 0.449
7.287 ± 0.217
1.908 ± 0.149
2.029 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here