Amino acid dipepetide frequency for Rhodococcus phage Whack

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.699AlaAla: 15.699 ± 1.566
0.638AlaCys: 0.638 ± 0.245
8.296AlaAsp: 8.296 ± 0.774
8.87AlaGlu: 8.87 ± 0.842
2.361AlaPhe: 2.361 ± 0.45
8.232AlaGly: 8.232 ± 1.11
2.489AlaHis: 2.489 ± 0.447
5.935AlaIle: 5.935 ± 0.631
5.361AlaLys: 5.361 ± 0.659
9.572AlaLeu: 9.572 ± 0.81
2.616AlaMet: 2.616 ± 0.414
3.701AlaAsn: 3.701 ± 0.8
5.807AlaPro: 5.807 ± 0.807
4.148AlaGln: 4.148 ± 0.604
6.445AlaArg: 6.445 ± 0.595
5.616AlaSer: 5.616 ± 0.521
6.637AlaThr: 6.637 ± 0.626
7.147AlaVal: 7.147 ± 0.584
2.616AlaTrp: 2.616 ± 0.613
1.914AlaTyr: 1.914 ± 0.274
0.0AlaXaa: 0.0 ± 0.0
Cys
0.574CysAla: 0.574 ± 0.207
0.064CysCys: 0.064 ± 0.059
0.574CysAsp: 0.574 ± 0.219
0.511CysGlu: 0.511 ± 0.195
0.255CysPhe: 0.255 ± 0.126
0.893CysGly: 0.893 ± 0.309
0.319CysHis: 0.319 ± 0.146
0.191CysIle: 0.191 ± 0.119
0.319CysLys: 0.319 ± 0.135
0.447CysLeu: 0.447 ± 0.164
0.255CysMet: 0.255 ± 0.122
0.319CysAsn: 0.319 ± 0.124
0.511CysPro: 0.511 ± 0.209
0.319CysGln: 0.319 ± 0.152
0.447CysArg: 0.447 ± 0.181
0.319CysSer: 0.319 ± 0.122
0.447CysThr: 0.447 ± 0.166
0.319CysVal: 0.319 ± 0.136
0.383CysTrp: 0.383 ± 0.175
0.064CysTyr: 0.064 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
7.147AspAla: 7.147 ± 0.799
0.638AspCys: 0.638 ± 0.178
4.786AspAsp: 4.786 ± 0.57
4.85AspGlu: 4.85 ± 0.652
2.234AspPhe: 2.234 ± 0.394
6.063AspGly: 6.063 ± 0.698
1.723AspHis: 1.723 ± 0.552
3.127AspIle: 3.127 ± 0.41
2.553AspLys: 2.553 ± 0.464
6.19AspLeu: 6.19 ± 0.672
1.532AspMet: 1.532 ± 0.308
1.468AspAsn: 1.468 ± 0.239
4.978AspPro: 4.978 ± 0.587
2.68AspGln: 2.68 ± 0.389
4.85AspArg: 4.85 ± 0.547
4.212AspSer: 4.212 ± 0.571
2.425AspThr: 2.425 ± 0.463
5.105AspVal: 5.105 ± 0.615
1.085AspTrp: 1.085 ± 0.22
1.468AspTyr: 1.468 ± 0.308
0.0AspXaa: 0.0 ± 0.0
Glu
7.211GluAla: 7.211 ± 0.703
0.447GluCys: 0.447 ± 0.178
3.957GluAsp: 3.957 ± 0.544
2.744GluGlu: 2.744 ± 0.479
2.042GluPhe: 2.042 ± 0.471
3.51GluGly: 3.51 ± 0.599
1.404GluHis: 1.404 ± 0.294
3.765GluIle: 3.765 ± 0.42
2.17GluLys: 2.17 ± 0.368
6.063GluLeu: 6.063 ± 0.651
1.659GluMet: 1.659 ± 0.36
1.468GluAsn: 1.468 ± 0.273
3.51GluPro: 3.51 ± 0.633
2.936GluGln: 2.936 ± 0.393
4.595GluArg: 4.595 ± 0.573
3.765GluSer: 3.765 ± 0.569
4.34GluThr: 4.34 ± 0.718
3.765GluVal: 3.765 ± 0.525
1.149GluTrp: 1.149 ± 0.287
0.702GluTyr: 0.702 ± 0.196
0.0GluXaa: 0.0 ± 0.0
Phe
2.936PheAla: 2.936 ± 0.486
0.128PheCys: 0.128 ± 0.094
3.446PheAsp: 3.446 ± 0.534
1.276PheGlu: 1.276 ± 0.306
0.893PhePhe: 0.893 ± 0.325
2.808PheGly: 2.808 ± 0.434
0.447PheHis: 0.447 ± 0.161
0.893PheIle: 0.893 ± 0.232
0.957PheLys: 0.957 ± 0.261
2.425PheLeu: 2.425 ± 0.376
0.574PheMet: 0.574 ± 0.167
0.383PheAsn: 0.383 ± 0.151
1.149PhePro: 1.149 ± 0.349
0.383PheGln: 0.383 ± 0.165
1.213PheArg: 1.213 ± 0.255
1.659PheSer: 1.659 ± 0.416
2.936PheThr: 2.936 ± 0.402
1.851PheVal: 1.851 ± 0.304
0.255PheTrp: 0.255 ± 0.103
0.383PheTyr: 0.383 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
6.892GlyAla: 6.892 ± 0.817
0.638GlyCys: 0.638 ± 0.234
6.254GlyAsp: 6.254 ± 1.492
4.34GlyGlu: 4.34 ± 0.483
2.361GlyPhe: 2.361 ± 0.449
6.254GlyGly: 6.254 ± 0.958
1.595GlyHis: 1.595 ± 0.305
5.233GlyIle: 5.233 ± 0.641
4.02GlyLys: 4.02 ± 0.488
8.105GlyLeu: 8.105 ± 0.973
1.978GlyMet: 1.978 ± 0.386
3.446GlyAsn: 3.446 ± 0.531
4.595GlyPro: 4.595 ± 0.625
2.042GlyGln: 2.042 ± 0.36
4.722GlyArg: 4.722 ± 0.67
4.467GlySer: 4.467 ± 0.589
5.041GlyThr: 5.041 ± 0.569
5.552GlyVal: 5.552 ± 0.596
2.106GlyTrp: 2.106 ± 0.38
2.17GlyTyr: 2.17 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.787HisAla: 1.787 ± 0.378
0.319HisCys: 0.319 ± 0.141
1.276HisAsp: 1.276 ± 0.249
1.276HisGlu: 1.276 ± 0.287
0.638HisPhe: 0.638 ± 0.182
1.404HisGly: 1.404 ± 0.408
0.511HisHis: 0.511 ± 0.186
0.83HisIle: 0.83 ± 0.248
0.574HisLys: 0.574 ± 0.198
1.787HisLeu: 1.787 ± 0.307
0.191HisMet: 0.191 ± 0.102
1.021HisAsn: 1.021 ± 0.239
1.978HisPro: 1.978 ± 0.429
0.766HisGln: 0.766 ± 0.227
1.532HisArg: 1.532 ± 0.339
1.276HisSer: 1.276 ± 0.271
0.893HisThr: 0.893 ± 0.224
1.404HisVal: 1.404 ± 0.331
0.319HisTrp: 0.319 ± 0.166
0.191HisTyr: 0.191 ± 0.117
0.0HisXaa: 0.0 ± 0.0
Ile
5.68IleAla: 5.68 ± 0.469
0.447IleCys: 0.447 ± 0.18
3.51IleAsp: 3.51 ± 0.536
3.318IleGlu: 3.318 ± 0.44
1.021IlePhe: 1.021 ± 0.308
4.148IleGly: 4.148 ± 0.634
0.766IleHis: 0.766 ± 0.242
2.297IleIle: 2.297 ± 0.455
2.489IleLys: 2.489 ± 0.414
4.084IleLeu: 4.084 ± 0.402
0.638IleMet: 0.638 ± 0.222
1.595IleAsn: 1.595 ± 0.368
2.553IlePro: 2.553 ± 0.34
1.851IleGln: 1.851 ± 0.298
4.212IleArg: 4.212 ± 0.417
2.616IleSer: 2.616 ± 0.375
3.318IleThr: 3.318 ± 0.562
2.936IleVal: 2.936 ± 0.398
0.766IleTrp: 0.766 ± 0.202
1.532IleTyr: 1.532 ± 0.397
0.0IleXaa: 0.0 ± 0.0
Lys
5.233LysAla: 5.233 ± 0.546
0.319LysCys: 0.319 ± 0.212
1.978LysAsp: 1.978 ± 0.397
1.723LysGlu: 1.723 ± 0.312
1.34LysPhe: 1.34 ± 0.236
3.893LysGly: 3.893 ± 0.993
0.511LysHis: 0.511 ± 0.188
1.595LysIle: 1.595 ± 0.298
1.34LysLys: 1.34 ± 0.353
3.446LysLeu: 3.446 ± 0.384
1.021LysMet: 1.021 ± 0.218
1.34LysAsn: 1.34 ± 0.247
2.744LysPro: 2.744 ± 0.441
1.213LysGln: 1.213 ± 0.225
2.489LysArg: 2.489 ± 0.578
2.553LysSer: 2.553 ± 0.449
2.17LysThr: 2.17 ± 0.357
3.255LysVal: 3.255 ± 0.404
0.702LysTrp: 0.702 ± 0.203
0.638LysTyr: 0.638 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
11.104LeuAla: 11.104 ± 0.885
0.511LeuCys: 0.511 ± 0.184
5.552LeuAsp: 5.552 ± 0.465
4.531LeuGlu: 4.531 ± 0.613
1.787LeuPhe: 1.787 ± 0.273
7.849LeuGly: 7.849 ± 1.099
0.957LeuHis: 0.957 ± 0.296
3.574LeuIle: 3.574 ± 0.408
3.063LeuLys: 3.063 ± 0.501
4.978LeuLeu: 4.978 ± 0.548
1.468LeuMet: 1.468 ± 0.287
2.297LeuAsn: 2.297 ± 0.341
4.084LeuPro: 4.084 ± 0.39
2.106LeuGln: 2.106 ± 0.371
5.552LeuArg: 5.552 ± 0.543
4.467LeuSer: 4.467 ± 0.479
5.488LeuThr: 5.488 ± 0.59
6.318LeuVal: 6.318 ± 0.72
1.34LeuTrp: 1.34 ± 0.314
2.106LeuTyr: 2.106 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.744MetAla: 2.744 ± 0.392
0.064MetCys: 0.064 ± 0.058
1.213MetAsp: 1.213 ± 0.253
0.511MetGlu: 0.511 ± 0.201
0.638MetPhe: 0.638 ± 0.2
1.213MetGly: 1.213 ± 0.291
0.319MetHis: 0.319 ± 0.155
1.149MetIle: 1.149 ± 0.248
0.766MetLys: 0.766 ± 0.203
0.957MetLeu: 0.957 ± 0.209
0.255MetMet: 0.255 ± 0.108
0.766MetAsn: 0.766 ± 0.17
1.404MetPro: 1.404 ± 0.246
1.085MetGln: 1.085 ± 0.265
1.276MetArg: 1.276 ± 0.271
2.616MetSer: 2.616 ± 0.396
2.553MetThr: 2.553 ± 0.437
1.723MetVal: 1.723 ± 0.369
0.319MetTrp: 0.319 ± 0.132
0.255MetTyr: 0.255 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
3.638AsnAla: 3.638 ± 0.549
0.064AsnCys: 0.064 ± 0.056
1.659AsnAsp: 1.659 ± 0.255
2.17AsnGlu: 2.17 ± 0.386
0.638AsnPhe: 0.638 ± 0.196
3.127AsnGly: 3.127 ± 0.395
0.447AsnHis: 0.447 ± 0.209
1.787AsnIle: 1.787 ± 0.409
1.021AsnLys: 1.021 ± 0.221
2.616AsnLeu: 2.616 ± 0.375
0.447AsnMet: 0.447 ± 0.168
0.638AsnAsn: 0.638 ± 0.16
3.574AsnPro: 3.574 ± 0.694
1.021AsnGln: 1.021 ± 0.325
1.978AsnArg: 1.978 ± 0.39
1.914AsnSer: 1.914 ± 0.396
1.595AsnThr: 1.595 ± 0.275
2.106AsnVal: 2.106 ± 0.374
0.574AsnTrp: 0.574 ± 0.138
0.511AsnTyr: 0.511 ± 0.161
0.0AsnXaa: 0.0 ± 0.0
Pro
6.19ProAla: 6.19 ± 0.622
0.574ProCys: 0.574 ± 0.197
3.957ProAsp: 3.957 ± 0.519
4.403ProGlu: 4.403 ± 0.67
1.978ProPhe: 1.978 ± 0.308
4.722ProGly: 4.722 ± 0.63
1.149ProHis: 1.149 ± 0.249
2.68ProIle: 2.68 ± 0.317
2.489ProLys: 2.489 ± 0.511
2.489ProLeu: 2.489 ± 0.374
1.34ProMet: 1.34 ± 0.261
2.425ProAsn: 2.425 ± 0.463
3.127ProPro: 3.127 ± 0.475
1.659ProGln: 1.659 ± 0.281
3.127ProArg: 3.127 ± 0.523
4.659ProSer: 4.659 ± 0.81
4.659ProThr: 4.659 ± 0.607
4.212ProVal: 4.212 ± 0.637
1.468ProTrp: 1.468 ± 0.318
1.532ProTyr: 1.532 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
3.893GlnAla: 3.893 ± 0.6
0.128GlnCys: 0.128 ± 0.089
1.532GlnAsp: 1.532 ± 0.292
1.404GlnGlu: 1.404 ± 0.276
1.085GlnPhe: 1.085 ± 0.259
2.106GlnGly: 2.106 ± 0.514
0.702GlnHis: 0.702 ± 0.227
1.723GlnIle: 1.723 ± 0.31
1.213GlnLys: 1.213 ± 0.324
3.127GlnLeu: 3.127 ± 0.352
0.957GlnMet: 0.957 ± 0.25
0.83GlnAsn: 0.83 ± 0.216
2.361GlnPro: 2.361 ± 0.32
1.149GlnGln: 1.149 ± 0.248
2.872GlnArg: 2.872 ± 0.407
1.659GlnSer: 1.659 ± 0.293
2.17GlnThr: 2.17 ± 0.428
2.553GlnVal: 2.553 ± 0.32
0.766GlnTrp: 0.766 ± 0.216
0.83GlnTyr: 0.83 ± 0.232
0.0GlnXaa: 0.0 ± 0.0
Arg
6.445ArgAla: 6.445 ± 0.696
0.702ArgCys: 0.702 ± 0.193
4.403ArgAsp: 4.403 ± 0.448
3.893ArgGlu: 3.893 ± 0.419
1.723ArgPhe: 1.723 ± 0.335
5.297ArgGly: 5.297 ± 0.803
1.659ArgHis: 1.659 ± 0.37
3.701ArgIle: 3.701 ± 0.413
1.914ArgLys: 1.914 ± 0.357
5.041ArgLeu: 5.041 ± 0.673
2.17ArgMet: 2.17 ± 0.33
2.17ArgAsn: 2.17 ± 0.397
3.127ArgPro: 3.127 ± 0.482
3.191ArgGln: 3.191 ± 0.442
5.233ArgArg: 5.233 ± 0.754
4.212ArgSer: 4.212 ± 0.462
4.02ArgThr: 4.02 ± 0.722
4.978ArgVal: 4.978 ± 0.516
0.893ArgTrp: 0.893 ± 0.248
1.532ArgTyr: 1.532 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
6.892SerAla: 6.892 ± 0.783
0.447SerCys: 0.447 ± 0.196
3.701SerAsp: 3.701 ± 0.46
2.808SerGlu: 2.808 ± 0.351
1.34SerPhe: 1.34 ± 0.302
5.871SerGly: 5.871 ± 0.571
1.149SerHis: 1.149 ± 0.266
2.744SerIle: 2.744 ± 0.351
2.553SerLys: 2.553 ± 0.298
4.467SerLeu: 4.467 ± 0.564
1.468SerMet: 1.468 ± 0.331
2.106SerAsn: 2.106 ± 0.272
3.638SerPro: 3.638 ± 0.52
1.659SerGln: 1.659 ± 0.365
4.212SerArg: 4.212 ± 0.548
4.148SerSer: 4.148 ± 0.418
4.531SerThr: 4.531 ± 0.544
4.914SerVal: 4.914 ± 0.599
1.021SerTrp: 1.021 ± 0.227
0.893SerTyr: 0.893 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
7.849ThrAla: 7.849 ± 0.65
0.383ThrCys: 0.383 ± 0.154
4.467ThrAsp: 4.467 ± 0.663
4.212ThrGlu: 4.212 ± 0.896
1.914ThrPhe: 1.914 ± 0.313
5.488ThrGly: 5.488 ± 0.788
1.532ThrHis: 1.532 ± 0.318
3.191ThrIle: 3.191 ± 0.565
2.234ThrLys: 2.234 ± 0.365
4.914ThrLeu: 4.914 ± 0.626
1.213ThrMet: 1.213 ± 0.304
1.34ThrAsn: 1.34 ± 0.436
4.34ThrPro: 4.34 ± 0.605
1.978ThrGln: 1.978 ± 0.335
4.084ThrArg: 4.084 ± 0.617
3.829ThrSer: 3.829 ± 0.508
4.531ThrThr: 4.531 ± 0.711
6.19ThrVal: 6.19 ± 0.611
1.149ThrTrp: 1.149 ± 0.363
1.659ThrTyr: 1.659 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
8.998ValAla: 8.998 ± 0.872
0.447ValCys: 0.447 ± 0.152
5.68ValAsp: 5.68 ± 0.498
5.233ValGlu: 5.233 ± 0.676
1.914ValPhe: 1.914 ± 0.398
5.552ValGly: 5.552 ± 0.596
1.276ValHis: 1.276 ± 0.281
3.51ValIle: 3.51 ± 0.449
2.425ValLys: 2.425 ± 0.308
5.233ValLeu: 5.233 ± 0.564
1.213ValMet: 1.213 ± 0.245
2.808ValAsn: 2.808 ± 0.456
3.318ValPro: 3.318 ± 0.439
1.914ValGln: 1.914 ± 0.429
4.722ValArg: 4.722 ± 0.515
4.276ValSer: 4.276 ± 0.613
5.68ValThr: 5.68 ± 0.813
5.871ValVal: 5.871 ± 0.729
1.468ValTrp: 1.468 ± 0.413
1.34ValTyr: 1.34 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
1.404TrpAla: 1.404 ± 0.325
0.319TrpCys: 0.319 ± 0.155
1.595TrpAsp: 1.595 ± 0.621
1.404TrpGlu: 1.404 ± 0.514
0.447TrpPhe: 0.447 ± 0.176
1.213TrpGly: 1.213 ± 0.268
0.574TrpHis: 0.574 ± 0.184
0.893TrpIle: 0.893 ± 0.2
1.085TrpLys: 1.085 ± 0.299
1.404TrpLeu: 1.404 ± 0.288
0.511TrpMet: 0.511 ± 0.168
0.702TrpAsn: 0.702 ± 0.348
1.404TrpPro: 1.404 ± 0.329
0.383TrpGln: 0.383 ± 0.151
1.34TrpArg: 1.34 ± 0.309
1.149TrpSer: 1.149 ± 0.347
1.404TrpThr: 1.404 ± 0.299
1.149TrpVal: 1.149 ± 0.262
0.383TrpTrp: 0.383 ± 0.15
0.319TrpTyr: 0.319 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.787TyrAla: 1.787 ± 0.348
0.255TyrCys: 0.255 ± 0.129
1.276TyrAsp: 1.276 ± 0.298
1.723TyrGlu: 1.723 ± 0.318
0.383TyrPhe: 0.383 ± 0.189
2.17TyrGly: 2.17 ± 0.398
0.447TyrHis: 0.447 ± 0.203
0.957TyrIle: 0.957 ± 0.251
0.957TyrLys: 0.957 ± 0.242
1.787TyrLeu: 1.787 ± 0.342
0.255TyrMet: 0.255 ± 0.095
0.702TyrAsn: 0.702 ± 0.189
0.702TyrPro: 0.702 ± 0.249
0.574TyrGln: 0.574 ± 0.213
1.404TyrArg: 1.404 ± 0.299
1.149TyrSer: 1.149 ± 0.295
1.659TyrThr: 1.659 ± 0.284
1.532TyrVal: 1.532 ± 0.339
0.319TyrTrp: 0.319 ± 0.146
0.255TyrTyr: 0.255 ± 0.135
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (15671 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski