Rothia mucilaginosa (strain DY-18) (Stomatococcus mucilaginosus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Rothia; Rothia mucilaginosa

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1991 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2NTU7|D2NTU7_ROTMD ABC-type dipeptide/oligopeptide/nickel transport system permease component OS=Rothia mucilaginosa (strain DY-18) OX=680646 GN=RMDY18_12410 PE=4 SV=1
MM1 pKa = 7.23WPWYY5 pKa = 8.79TPSGNLARR13 pKa = 11.84IPRR16 pKa = 11.84FVILFSVLRR25 pKa = 11.84GGVLDD30 pKa = 4.66DD31 pKa = 5.45DD32 pKa = 4.63GVAVDD37 pKa = 4.45VEE39 pKa = 4.75SLTVPVSHH47 pKa = 7.28ASLLGVLDD55 pKa = 3.78SFEE58 pKa = 4.25EE59 pKa = 4.17KK60 pKa = 10.71LVGVFNGQGCEE71 pKa = 3.89GLLLTEE77 pKa = 4.83PLVEE81 pKa = 4.75GNDD84 pKa = 3.71GCGGFDD90 pKa = 3.35QVAVGTDD97 pKa = 3.18VEE99 pKa = 4.76FTLRR103 pKa = 11.84TSDD106 pKa = 3.93SVEE109 pKa = 3.9AEE111 pKa = 4.16CGKK114 pKa = 10.34EE115 pKa = 3.93CTRR118 pKa = 11.84SNVLGFVGTEE128 pKa = 3.71AQLTQGGEE136 pKa = 4.06AQNPGTDD143 pKa = 3.59DD144 pKa = 3.21TAACDD149 pKa = 3.55GTGFFEE155 pKa = 4.69RR156 pKa = 11.84GRR158 pKa = 11.84DD159 pKa = 3.42ATQAEE164 pKa = 4.94SGSGDD169 pKa = 3.21AGVSLGGLYY178 pKa = 10.27FLAACGNVLGAQLSFLVIDD197 pKa = 4.02EE198 pKa = 4.25RR199 pKa = 11.84VKK201 pKa = 10.42FLSVKK206 pKa = 10.3LYY208 pKa = 9.55TGGYY212 pKa = 9.44GINTGTATACLSVFSAGFLVCEE234 pKa = 4.73DD235 pKa = 4.43ADD237 pKa = 4.14DD238 pKa = 4.18FTDD241 pKa = 3.5EE242 pKa = 4.45GAAQAHH248 pKa = 6.0LVALASRR255 pKa = 11.84VPRR258 pKa = 11.84TEE260 pKa = 3.68EE261 pKa = 3.85EE262 pKa = 4.22RR263 pKa = 11.84CGGGVEE269 pKa = 4.31QCAHH273 pKa = 5.14VCGGAVAQCAVLVVFGDD290 pKa = 3.89GLTVDD295 pKa = 3.49VTGTEE300 pKa = 4.21VAIEE304 pKa = 3.97DD305 pKa = 3.84AVGEE309 pKa = 3.9FHH311 pKa = 7.42VGVFDD316 pKa = 3.64GTVFGLVGDD325 pKa = 4.9DD326 pKa = 3.81GAGNLDD332 pKa = 3.63DD333 pKa = 5.77CFVAVLAALFDD344 pKa = 4.25GGFCTVEE351 pKa = 3.78FRR353 pKa = 11.84LLAVNEE359 pKa = 4.19DD360 pKa = 3.75DD361 pKa = 5.54LLRR364 pKa = 11.84LALDD368 pKa = 3.7GLLGFVLVFRR378 pKa = 11.84LLYY381 pKa = 10.55DD382 pKa = 3.34VADD385 pKa = 4.84FVCEE389 pKa = 4.07DD390 pKa = 3.99RR391 pKa = 11.84LTVAPLTIDD400 pKa = 4.21KK401 pKa = 11.07LGGCQGCVGACCRR414 pKa = 11.84FLFSDD419 pKa = 4.11RR420 pKa = 11.84GDD422 pKa = 3.38NGAATEE428 pKa = 4.4LDD430 pKa = 3.58VLDD433 pKa = 4.49EE434 pKa = 4.37VGEE437 pKa = 4.35GDD439 pKa = 4.65DD440 pKa = 4.83GLGLVLGHH448 pKa = 6.7GCLLRR453 pKa = 5.67

Molecular weight: 46.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2NQD8|D2NQD8_ROTMD EIIAB-Man OS=Rothia mucilaginosa (strain DY-18) OX=680646 GN=RMDY18_00320 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSSRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1991

0

1991

757693

30

2358

380.6

41.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.593 ± 0.058

1.054 ± 0.032

5.012 ± 0.046

6.403 ± 0.06

3.055 ± 0.036

8.59 ± 0.072

2.647 ± 0.047

4.408 ± 0.05

3.141 ± 0.048

9.801 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.029

3.337 ± 0.033

4.653 ± 0.05

3.841 ± 0.046

6.598 ± 0.06

6.348 ± 0.066

5.982 ± 0.048

8.207 ± 0.054

1.006 ± 0.022

2.379 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski