Sandaracinus amylolyticus
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8941 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6YI92|A0A0F6YI92_9DELT Uncharacterized protein OS=Sandaracinus amylolyticus OX=927083 GN=DB32_003884 PE=4 SV=1
MM1 pKa = 7.3 CRR3 pKa = 11.84 WHH5 pKa = 7.34 LDD7 pKa = 3.32 HH8 pKa = 7.36 ASCADD13 pKa = 3.98 DD14 pKa = 3.98 VFCDD18 pKa = 3.74 GAEE21 pKa = 3.9 RR22 pKa = 11.84 CDD24 pKa = 3.84 PVDD27 pKa = 3.12 GCIEE31 pKa = 3.94 GRR33 pKa = 11.84 RR34 pKa = 11.84 EE35 pKa = 3.93 SCDD38 pKa = 3.6 DD39 pKa = 3.8 GDD41 pKa = 3.69 VCTVDD46 pKa = 3.01 RR47 pKa = 11.84 CVEE50 pKa = 4.13 SEE52 pKa = 4.2 ASCTHH57 pKa = 7.29 AARR60 pKa = 11.84 DD61 pKa = 3.84 LDD63 pKa = 4.33 DD64 pKa = 6.24 DD65 pKa = 5.02 GDD67 pKa = 3.66 VDD69 pKa = 4.02 MFCDD73 pKa = 4.97 GGNDD77 pKa = 4.5 CDD79 pKa = 5.2 DD80 pKa = 4.82 ADD82 pKa = 4.08 PTVSSGTDD90 pKa = 3.22 EE91 pKa = 4.32 RR92 pKa = 11.84 CGNRR96 pKa = 11.84 RR97 pKa = 11.84 DD98 pKa = 4.05 DD99 pKa = 4.8 DD100 pKa = 4.36 CDD102 pKa = 4.53 GDD104 pKa = 3.86 VDD106 pKa = 4.32 EE107 pKa = 5.72 DD108 pKa = 3.91 ACATAPHH115 pKa = 6.78 DD116 pKa = 3.99 TCDD119 pKa = 4.21 DD120 pKa = 4.32 PLDD123 pKa = 3.85 VSAGGSFVIEE133 pKa = 3.92 ARR135 pKa = 11.84 EE136 pKa = 3.89 LRR138 pKa = 11.84 GDD140 pKa = 3.69 YY141 pKa = 10.22 LGCGILEE148 pKa = 4.34 CRR150 pKa = 11.84 DD151 pKa = 3.4 FVARR155 pKa = 11.84 FTIDD159 pKa = 2.96 EE160 pKa = 4.4 AQDD163 pKa = 3.58 VEE165 pKa = 5.65 IIAADD170 pKa = 4.28 PGDD173 pKa = 3.5 DD174 pKa = 3.21 WAYY177 pKa = 10.06 TEE179 pKa = 3.95 VALRR183 pKa = 11.84 RR184 pKa = 11.84 GCDD187 pKa = 3.38 DD188 pKa = 3.41 ATTEE192 pKa = 4.15 IDD194 pKa = 4.27 DD195 pKa = 3.89 VTGFPATLRR204 pKa = 11.84 YY205 pKa = 9.12 RR206 pKa = 11.84 ALEE209 pKa = 4.21 PGTYY213 pKa = 9.4 FVLAGAEE220 pKa = 4.17 RR221 pKa = 11.84 NFGSGATDD229 pKa = 3.31 QLTLDD234 pKa = 3.82 VTFSPDD240 pKa = 3.05 TTPPTNEE247 pKa = 3.74 TCASALDD254 pKa = 3.9 VGAGGTFTADD264 pKa = 4.45 FIDD267 pKa = 3.81 TRR269 pKa = 11.84 DD270 pKa = 4.1 DD271 pKa = 3.58 LALEE275 pKa = 4.64 CGEE278 pKa = 4.9 DD279 pKa = 3.47 DD280 pKa = 3.91 WPEE283 pKa = 3.52 LVYY286 pKa = 10.69 AITTSARR293 pKa = 11.84 ADD295 pKa = 3.46 LRR297 pKa = 11.84 VTLDD301 pKa = 3.01 GFARR305 pKa = 11.84 LGLSVRR311 pKa = 11.84 SACGDD316 pKa = 3.24 ATSALGCVVLEE327 pKa = 3.99 PHH329 pKa = 6.85 EE330 pKa = 4.45 DD331 pKa = 3.42 RR332 pKa = 11.84 VRR334 pKa = 11.84 VFPDD338 pKa = 3.57 VPAGTHH344 pKa = 5.16 YY345 pKa = 10.44 FVLEE349 pKa = 3.94 LRR351 pKa = 11.84 SIPEE355 pKa = 4.7 AIAQITIEE363 pKa = 4.73 ALPAAPP369 pKa = 4.86
Molecular weight: 39.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.923
Patrickios 1.456
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A0F6W5Z7|A0A0F6W5Z7_9DELT Histidine kinase OS=Sandaracinus amylolyticus OX=927083 GN=DB32_005441 PE=4 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 SARR5 pKa = 11.84 GSARR9 pKa = 11.84 RR10 pKa = 11.84 PPRR13 pKa = 11.84 GRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 ASTSTSPSAWRR28 pKa = 11.84 AVPARR33 pKa = 11.84 PRR35 pKa = 11.84 AEE37 pKa = 3.83 QRR39 pKa = 11.84 PSVPTGRR46 pKa = 11.84 HH47 pKa = 3.97 AAIARR52 pKa = 11.84 AAQARR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 GPARR63 pKa = 11.84 PIAGRR68 pKa = 11.84 ARR70 pKa = 11.84 AATAPVALRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 APPVRR86 pKa = 11.84 PTARR90 pKa = 11.84 PAPTASAPPASRR102 pKa = 11.84 SRR104 pKa = 11.84 RR105 pKa = 11.84 APRR108 pKa = 11.84 TAQSAEE114 pKa = 3.98 TARR117 pKa = 11.84 ARR119 pKa = 11.84 PAPSRR124 pKa = 11.84 PRR126 pKa = 11.84 AARR129 pKa = 11.84 RR130 pKa = 11.84 TALRR134 pKa = 11.84 AVKK137 pKa = 9.78 PRR139 pKa = 11.84 ARR141 pKa = 11.84 AARR144 pKa = 11.84 RR145 pKa = 11.84 ASSARR150 pKa = 11.84 STPCARR156 pKa = 11.84 RR157 pKa = 11.84 RR158 pKa = 11.84 GSTARR163 pKa = 11.84 RR164 pKa = 11.84 RR165 pKa = 11.84 PAARR169 pKa = 11.84 RR170 pKa = 11.84 SS171 pKa = 3.39
Molecular weight: 18.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.525
Rodwell 12.559
Grimsley 13.086
Solomon 13.554
Lehninger 13.451
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.266
IPC_peptide 13.554
IPC2_peptide 12.544
IPC2.peptide.svr19 9.241
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8941
0
8941
3139928
37
3731
351.2
37.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.858 ± 0.046
1.331 ± 0.03
6.482 ± 0.029
6.542 ± 0.023
2.827 ± 0.015
8.793 ± 0.038
2.191 ± 0.012
3.889 ± 0.014
1.539 ± 0.022
9.604 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.842 ± 0.012
1.414 ± 0.014
5.796 ± 0.024
2.333 ± 0.015
9.455 ± 0.038
5.36 ± 0.02
5.297 ± 0.024
8.361 ± 0.025
1.369 ± 0.011
1.718 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here