Bamboo mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q89760|Q89760_9VIRU Coat protein OS=Bamboo mosaic virus OX=35286 GN=CP PE=3 SV=1
MM1 pKa = 7.68LNTDD5 pKa = 4.12TLCIILFILILGILYY20 pKa = 10.49NILQQHH26 pKa = 6.87LPPPCEE32 pKa = 3.99IIINGHH38 pKa = 5.5TISIRR43 pKa = 11.84GNCYY47 pKa = 8.75HH48 pKa = 6.05TTSSS52 pKa = 3.38

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65007|Q65007_9VIRU ORF2=28k OS=Bamboo mosaic virus OX=35286 PE=4 SV=1
MM1 pKa = 8.07PLTCSSSRR9 pKa = 11.84SSASITQTQQLPKK22 pKa = 10.3PQTLNNKK29 pKa = 9.43KK30 pKa = 9.86VDD32 pKa = 3.84QNPTPTSRR40 pKa = 11.84SPFSKK45 pKa = 10.11PNAPRR50 pKa = 11.84TRR52 pKa = 11.84KK53 pKa = 9.86CPHH56 pKa = 5.96HH57 pKa = 6.61QNAKK61 pKa = 9.41RR62 pKa = 11.84LKK64 pKa = 9.49LRR66 pKa = 11.84KK67 pKa = 9.35RR68 pKa = 11.84PTKK71 pKa = 9.92QGPVQTPLSTLTKK84 pKa = 9.76RR85 pKa = 11.84QPRR88 pKa = 11.84QNPQTARR95 pKa = 11.84SGSAPHH101 pKa = 6.76CVNCHH106 pKa = 4.36GRR108 pKa = 11.84RR109 pKa = 11.84GSNYY113 pKa = 9.89FGALDD118 pKa = 4.13SKK120 pKa = 10.94ATLNSWTT127 pKa = 3.96

Molecular weight: 14.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2158

52

1365

359.7

40.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.387 ± 0.666

1.622 ± 0.271

4.773 ± 0.421

5.375 ± 1.572

3.29 ± 0.466

5.514 ± 1.293

3.29 ± 0.543

5.792 ± 0.771

6.395 ± 1.086

9.731 ± 0.695

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.576 ± 0.247

4.449 ± 0.532

7.414 ± 0.867

4.495 ± 0.464

4.773 ± 0.471

5.607 ± 0.53

8.758 ± 1.212

3.846 ± 0.54

1.251 ± 0.246

3.661 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski