Pectobacterium phage PP99

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gajwadongvirus; Pectobacterium virus PP99

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8L656|A0A1P8L656_9CAUD Uncharacterized protein OS=Pectobacterium phage PP99 OX=1932883 GN=PP99_49 PE=4 SV=1
MM1 pKa = 7.27NFSFTRR7 pKa = 11.84DD8 pKa = 2.99TGDD11 pKa = 3.02GVKK14 pKa = 9.33TVFTMSFAGQDD25 pKa = 3.16EE26 pKa = 5.55GYY28 pKa = 10.38LNSSNIHH35 pKa = 5.05VLVNGVEE42 pKa = 4.43VPFTINPTDD51 pKa = 3.75PNKK54 pKa = 10.24VYY56 pKa = 11.06LSTAPANGADD66 pKa = 3.53VLIRR70 pKa = 11.84RR71 pKa = 11.84IMPKK75 pKa = 9.89NVPYY79 pKa = 11.04SDD81 pKa = 4.23FKK83 pKa = 11.33NGNPFSQDD91 pKa = 2.88TLNYY95 pKa = 8.15TQLQQLYY102 pKa = 7.8VTQEE106 pKa = 3.81ILDD109 pKa = 3.97GFLPDD114 pKa = 4.2GFYY117 pKa = 10.87FKK119 pKa = 10.84QDD121 pKa = 3.38VNMGGYY127 pKa = 9.75NIKK130 pKa = 10.69NLGDD134 pKa = 3.8AVDD137 pKa = 4.65PDD139 pKa = 3.98DD140 pKa = 4.41AVKK143 pKa = 10.91KK144 pKa = 10.44EE145 pKa = 3.97VTDD148 pKa = 3.88DD149 pKa = 3.35LGNRR153 pKa = 11.84VSSLEE158 pKa = 4.29DD159 pKa = 3.32NLTDD163 pKa = 3.46TSLTTVPWRR172 pKa = 11.84FVATGGEE179 pKa = 4.6VTVSPPYY186 pKa = 10.87SFTNALLYY194 pKa = 10.62INGVTQTLGADD205 pKa = 3.41YY206 pKa = 10.57AYY208 pKa = 9.39TVSGNQLHH216 pKa = 6.45LPEE219 pKa = 4.91PLRR222 pKa = 11.84AGDD225 pKa = 3.77EE226 pKa = 4.25VFVIIGTNIQPPSDD240 pKa = 4.38LISYY244 pKa = 10.05DD245 pKa = 3.38GALIAYY251 pKa = 9.21ASMVTGVPVQQVYY264 pKa = 10.42IPVVGLTVSNALVFIDD280 pKa = 3.66NGVIHH285 pKa = 6.71TLNTPASGVITSIAWPDD302 pKa = 3.27IVISS306 pKa = 3.82

Molecular weight: 33.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8L618|A0A1P8L618_9CAUD Uncharacterized protein OS=Pectobacterium phage PP99 OX=1932883 GN=PP99_06 PE=4 SV=1
MM1 pKa = 7.69TYY3 pKa = 11.26LLIAACGWLVFIYY16 pKa = 10.18KK17 pKa = 9.45VRR19 pKa = 11.84RR20 pKa = 11.84DD21 pKa = 3.59FKK23 pKa = 10.74KK24 pKa = 9.67AHH26 pKa = 5.25EE27 pKa = 4.76RR28 pKa = 11.84YY29 pKa = 10.17NIVKK33 pKa = 10.48RR34 pKa = 11.84GDD36 pKa = 3.0NWVVRR41 pKa = 11.84DD42 pKa = 3.68SKK44 pKa = 11.54GRR46 pKa = 11.84FVTITDD52 pKa = 3.93NYY54 pKa = 10.3WNICKK59 pKa = 10.47LGAA62 pKa = 4.04

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13584

49

1288

242.6

27.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.782 ± 0.437

1.016 ± 0.162

6.287 ± 0.177

6.419 ± 0.353

3.585 ± 0.166

7.052 ± 0.256

1.973 ± 0.22

5.102 ± 0.177

6.603 ± 0.244

8.444 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.217 ± 0.204

4.557 ± 0.298

3.651 ± 0.178

4.594 ± 0.294

4.726 ± 0.226

5.978 ± 0.284

6.022 ± 0.314

6.751 ± 0.311

1.369 ± 0.137

3.872 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski