Amino acid dipepetide frequency for Pectobacterium phage PP99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.055AlaAla: 9.055 ± 1.433
0.81AlaCys: 0.81 ± 0.312
5.006AlaAsp: 5.006 ± 0.66
6.037AlaGlu: 6.037 ± 0.58
3.092AlaPhe: 3.092 ± 0.487
6.552AlaGly: 6.552 ± 0.754
1.399AlaHis: 1.399 ± 0.217
5.08AlaIle: 5.08 ± 0.555
6.479AlaLys: 6.479 ± 0.857
5.301AlaLeu: 5.301 ± 0.544
3.313AlaMet: 3.313 ± 0.457
4.196AlaAsn: 4.196 ± 0.522
2.135AlaPro: 2.135 ± 0.372
4.933AlaGln: 4.933 ± 0.642
3.534AlaArg: 3.534 ± 0.584
4.859AlaSer: 4.859 ± 0.447
5.154AlaThr: 5.154 ± 0.617
6.479AlaVal: 6.479 ± 0.7
1.104AlaTrp: 1.104 ± 0.273
3.313AlaTyr: 3.313 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.368CysAla: 0.368 ± 0.179
0.147CysCys: 0.147 ± 0.1
0.515CysAsp: 0.515 ± 0.218
0.368CysGlu: 0.368 ± 0.146
0.515CysPhe: 0.515 ± 0.247
0.515CysGly: 0.515 ± 0.213
0.221CysHis: 0.221 ± 0.134
0.957CysIle: 0.957 ± 0.244
0.81CysLys: 0.81 ± 0.238
0.663CysLeu: 0.663 ± 0.22
0.368CysMet: 0.368 ± 0.19
0.368CysAsn: 0.368 ± 0.198
0.589CysPro: 0.589 ± 0.171
0.294CysGln: 0.294 ± 0.137
0.883CysArg: 0.883 ± 0.224
0.589CysSer: 0.589 ± 0.207
0.589CysThr: 0.589 ± 0.197
0.663CysVal: 0.663 ± 0.229
0.074CysTrp: 0.074 ± 0.074
0.663CysTyr: 0.663 ± 0.253
0.0CysXaa: 0.0 ± 0.0
Asp
5.742AspAla: 5.742 ± 0.57
0.736AspCys: 0.736 ± 0.25
3.534AspAsp: 3.534 ± 0.467
4.565AspGlu: 4.565 ± 0.684
2.577AspPhe: 2.577 ± 0.477
5.448AspGly: 5.448 ± 0.544
0.957AspHis: 0.957 ± 0.269
3.092AspIle: 3.092 ± 0.519
3.46AspLys: 3.46 ± 0.376
5.448AspLeu: 5.448 ± 0.414
1.62AspMet: 1.62 ± 0.314
3.46AspAsn: 3.46 ± 0.545
2.503AspPro: 2.503 ± 0.429
1.841AspGln: 1.841 ± 0.282
2.503AspArg: 2.503 ± 0.521
3.534AspSer: 3.534 ± 0.543
3.902AspThr: 3.902 ± 0.437
3.755AspVal: 3.755 ± 0.5
1.399AspTrp: 1.399 ± 0.322
2.798AspTyr: 2.798 ± 0.512
0.0AspXaa: 0.0 ± 0.0
Glu
6.773GluAla: 6.773 ± 0.849
0.515GluCys: 0.515 ± 0.168
3.313GluAsp: 3.313 ± 0.588
3.828GluGlu: 3.828 ± 0.504
2.356GluPhe: 2.356 ± 0.426
3.902GluGly: 3.902 ± 0.49
1.252GluHis: 1.252 ± 0.276
2.282GluIle: 2.282 ± 0.549
3.681GluLys: 3.681 ± 0.602
6.994GluLeu: 6.994 ± 0.746
1.914GluMet: 1.914 ± 0.294
1.767GluAsn: 1.767 ± 0.31
1.914GluPro: 1.914 ± 0.343
4.638GluGln: 4.638 ± 0.482
3.681GluArg: 3.681 ± 0.508
3.607GluSer: 3.607 ± 0.487
2.798GluThr: 2.798 ± 0.537
4.933GluVal: 4.933 ± 0.61
1.472GluTrp: 1.472 ± 0.382
2.577GluTyr: 2.577 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
2.43PheAla: 2.43 ± 0.497
0.589PheCys: 0.589 ± 0.217
2.43PheAsp: 2.43 ± 0.423
2.282PheGlu: 2.282 ± 0.502
1.472PhePhe: 1.472 ± 0.314
2.724PheGly: 2.724 ± 0.451
0.515PheHis: 0.515 ± 0.234
2.43PheIle: 2.43 ± 0.3
2.43PheLys: 2.43 ± 0.34
2.65PheLeu: 2.65 ± 0.462
1.252PheMet: 1.252 ± 0.283
3.018PheAsn: 3.018 ± 0.342
0.883PhePro: 0.883 ± 0.333
0.957PheGln: 0.957 ± 0.183
1.767PheArg: 1.767 ± 0.364
1.914PheSer: 1.914 ± 0.329
2.209PheThr: 2.209 ± 0.434
2.43PheVal: 2.43 ± 0.377
0.221PheTrp: 0.221 ± 0.12
1.252PheTyr: 1.252 ± 0.433
0.0PheXaa: 0.0 ± 0.0
Gly
4.933GlyAla: 4.933 ± 0.778
1.031GlyCys: 1.031 ± 0.297
5.374GlyAsp: 5.374 ± 0.649
4.565GlyGlu: 4.565 ± 0.597
3.018GlyPhe: 3.018 ± 0.427
4.565GlyGly: 4.565 ± 0.655
1.399GlyHis: 1.399 ± 0.363
3.46GlyIle: 3.46 ± 0.428
5.89GlyLys: 5.89 ± 0.859
6.479GlyLeu: 6.479 ± 0.622
1.914GlyMet: 1.914 ± 0.457
3.092GlyAsn: 3.092 ± 0.474
0.074GlyPro: 0.074 ± 0.059
2.577GlyGln: 2.577 ± 0.483
3.46GlyArg: 3.46 ± 0.519
3.681GlySer: 3.681 ± 0.508
5.08GlyThr: 5.08 ± 0.702
5.374GlyVal: 5.374 ± 0.878
1.104GlyTrp: 1.104 ± 0.229
3.46GlyTyr: 3.46 ± 0.508
0.0GlyXaa: 0.0 ± 0.0
His
1.031HisAla: 1.031 ± 0.282
0.147HisCys: 0.147 ± 0.111
1.399HisAsp: 1.399 ± 0.294
1.178HisGlu: 1.178 ± 0.273
0.442HisPhe: 0.442 ± 0.199
1.62HisGly: 1.62 ± 0.257
0.663HisHis: 0.663 ± 0.284
0.736HisIle: 0.736 ± 0.226
1.914HisLys: 1.914 ± 0.415
2.061HisLeu: 2.061 ± 0.396
0.663HisMet: 0.663 ± 0.248
0.442HisAsn: 0.442 ± 0.169
0.736HisPro: 0.736 ± 0.271
0.736HisGln: 0.736 ± 0.221
1.178HisArg: 1.178 ± 0.359
0.736HisSer: 0.736 ± 0.213
1.546HisThr: 1.546 ± 0.342
1.325HisVal: 1.325 ± 0.308
0.294HisTrp: 0.294 ± 0.137
0.883HisTyr: 0.883 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
5.006IleAla: 5.006 ± 0.535
0.515IleCys: 0.515 ± 0.224
3.387IleAsp: 3.387 ± 0.469
3.092IleGlu: 3.092 ± 0.432
1.472IlePhe: 1.472 ± 0.358
3.239IleGly: 3.239 ± 0.446
1.399IleHis: 1.399 ± 0.324
2.577IleIle: 2.577 ± 0.471
3.828IleLys: 3.828 ± 0.489
3.681IleLeu: 3.681 ± 0.397
1.399IleMet: 1.399 ± 0.283
3.239IleAsn: 3.239 ± 0.54
1.988IlePro: 1.988 ± 0.44
1.693IleGln: 1.693 ± 0.371
2.65IleArg: 2.65 ± 0.405
3.018IleSer: 3.018 ± 0.428
3.976IleThr: 3.976 ± 0.571
2.871IleVal: 2.871 ± 0.442
0.515IleTrp: 0.515 ± 0.183
1.472IleTyr: 1.472 ± 0.311
0.0IleXaa: 0.0 ± 0.0
Lys
6.7LysAla: 6.7 ± 0.794
0.442LysCys: 0.442 ± 0.174
4.123LysAsp: 4.123 ± 0.552
4.785LysGlu: 4.785 ± 0.597
2.061LysPhe: 2.061 ± 0.315
4.712LysGly: 4.712 ± 0.559
0.957LysHis: 0.957 ± 0.289
2.503LysIle: 2.503 ± 0.384
3.166LysLys: 3.166 ± 0.475
6.773LysLeu: 6.773 ± 0.761
2.061LysMet: 2.061 ± 0.317
2.061LysAsn: 2.061 ± 0.35
3.755LysPro: 3.755 ± 0.608
3.018LysGln: 3.018 ± 0.456
3.681LysArg: 3.681 ± 0.531
3.534LysSer: 3.534 ± 0.486
2.798LysThr: 2.798 ± 0.268
4.933LysVal: 4.933 ± 0.579
1.031LysTrp: 1.031 ± 0.287
2.871LysTyr: 2.871 ± 0.403
0.0LysXaa: 0.0 ± 0.0
Leu
8.098LeuAla: 8.098 ± 0.711
0.442LeuCys: 0.442 ± 0.193
5.522LeuAsp: 5.522 ± 0.552
5.89LeuGlu: 5.89 ± 0.763
2.503LeuPhe: 2.503 ± 0.417
5.595LeuGly: 5.595 ± 0.6
2.061LeuHis: 2.061 ± 0.449
4.196LeuIle: 4.196 ± 0.52
5.154LeuLys: 5.154 ± 0.524
5.963LeuLeu: 5.963 ± 0.851
3.092LeuMet: 3.092 ± 0.412
3.387LeuAsn: 3.387 ± 0.461
4.123LeuPro: 4.123 ± 0.414
3.46LeuGln: 3.46 ± 0.536
4.344LeuArg: 4.344 ± 0.56
5.816LeuSer: 5.816 ± 0.78
5.448LeuThr: 5.448 ± 0.643
5.522LeuVal: 5.522 ± 0.587
1.031LeuTrp: 1.031 ± 0.295
2.798LeuTyr: 2.798 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
2.724MetAla: 2.724 ± 0.34
0.294MetCys: 0.294 ± 0.148
1.399MetAsp: 1.399 ± 0.301
2.209MetGlu: 2.209 ± 0.63
0.957MetPhe: 0.957 ± 0.212
1.988MetGly: 1.988 ± 0.347
0.736MetHis: 0.736 ± 0.217
1.104MetIle: 1.104 ± 0.274
2.724MetLys: 2.724 ± 0.449
2.577MetLeu: 2.577 ± 0.312
1.325MetMet: 1.325 ± 0.296
1.325MetAsn: 1.325 ± 0.225
1.178MetPro: 1.178 ± 0.285
1.841MetGln: 1.841 ± 0.436
1.546MetArg: 1.546 ± 0.281
2.724MetSer: 2.724 ± 0.483
2.061MetThr: 2.061 ± 0.38
1.767MetVal: 1.767 ± 0.332
0.442MetTrp: 0.442 ± 0.203
1.252MetTyr: 1.252 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
2.945AsnAla: 2.945 ± 0.521
0.442AsnCys: 0.442 ± 0.235
2.282AsnAsp: 2.282 ± 0.343
2.282AsnGlu: 2.282 ± 0.354
1.399AsnPhe: 1.399 ± 0.33
3.828AsnGly: 3.828 ± 0.758
0.663AsnHis: 0.663 ± 0.23
3.018AsnIle: 3.018 ± 0.509
3.387AsnLys: 3.387 ± 0.535
4.417AsnLeu: 4.417 ± 0.585
1.472AsnMet: 1.472 ± 0.284
2.282AsnAsn: 2.282 ± 0.505
2.209AsnPro: 2.209 ± 0.371
2.135AsnGln: 2.135 ± 0.326
2.65AsnArg: 2.65 ± 0.398
2.061AsnSer: 2.061 ± 0.382
2.945AsnThr: 2.945 ± 0.468
2.65AsnVal: 2.65 ± 0.524
0.736AsnTrp: 0.736 ± 0.198
1.767AsnTyr: 1.767 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
3.828ProAla: 3.828 ± 0.397
0.442ProCys: 0.442 ± 0.205
2.724ProAsp: 2.724 ± 0.456
3.681ProGlu: 3.681 ± 0.481
1.252ProPhe: 1.252 ± 0.259
0.147ProGly: 0.147 ± 0.103
0.736ProHis: 0.736 ± 0.245
1.988ProIle: 1.988 ± 0.581
1.767ProLys: 1.767 ± 0.38
2.282ProLeu: 2.282 ± 0.458
0.883ProMet: 0.883 ± 0.223
1.767ProAsn: 1.767 ± 0.376
1.62ProPro: 1.62 ± 0.332
1.988ProGln: 1.988 ± 0.364
0.81ProArg: 0.81 ± 0.219
2.135ProSer: 2.135 ± 0.399
2.871ProThr: 2.871 ± 0.342
3.239ProVal: 3.239 ± 0.468
0.515ProTrp: 0.515 ± 0.179
1.841ProTyr: 1.841 ± 0.527
0.0ProXaa: 0.0 ± 0.0
Gln
5.448GlnAla: 5.448 ± 0.77
0.368GlnCys: 0.368 ± 0.167
2.282GlnAsp: 2.282 ± 0.41
3.018GlnGlu: 3.018 ± 0.357
1.988GlnPhe: 1.988 ± 0.27
2.724GlnGly: 2.724 ± 0.624
0.957GlnHis: 0.957 ± 0.292
1.399GlnIle: 1.399 ± 0.305
2.356GlnLys: 2.356 ± 0.406
5.227GlnLeu: 5.227 ± 0.599
1.988GlnMet: 1.988 ± 0.51
1.693GlnAsn: 1.693 ± 0.518
1.178GlnPro: 1.178 ± 0.318
3.092GlnGln: 3.092 ± 0.561
2.061GlnArg: 2.061 ± 0.376
3.46GlnSer: 3.46 ± 0.547
1.767GlnThr: 1.767 ± 0.349
3.166GlnVal: 3.166 ± 0.449
0.515GlnTrp: 0.515 ± 0.178
1.252GlnTyr: 1.252 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
4.049ArgAla: 4.049 ± 0.59
0.515ArgCys: 0.515 ± 0.195
3.387ArgAsp: 3.387 ± 0.428
3.534ArgGlu: 3.534 ± 0.583
2.135ArgPhe: 2.135 ± 0.33
4.049ArgGly: 4.049 ± 0.391
0.883ArgHis: 0.883 ± 0.222
2.724ArgIle: 2.724 ± 0.408
3.166ArgLys: 3.166 ± 0.552
3.681ArgLeu: 3.681 ± 0.358
1.914ArgMet: 1.914 ± 0.41
1.693ArgAsn: 1.693 ± 0.304
1.693ArgPro: 1.693 ± 0.289
1.693ArgGln: 1.693 ± 0.31
1.62ArgArg: 1.62 ± 0.3
2.871ArgSer: 2.871 ± 0.467
1.988ArgThr: 1.988 ± 0.301
3.018ArgVal: 3.018 ± 0.376
0.589ArgTrp: 0.589 ± 0.22
2.061ArgTyr: 2.061 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
3.976SerAla: 3.976 ± 0.645
0.81SerCys: 0.81 ± 0.258
4.049SerAsp: 4.049 ± 0.506
3.387SerGlu: 3.387 ± 0.505
2.209SerPhe: 2.209 ± 0.382
5.448SerGly: 5.448 ± 0.652
0.736SerHis: 0.736 ± 0.241
3.828SerIle: 3.828 ± 0.536
4.049SerLys: 4.049 ± 0.537
5.374SerLeu: 5.374 ± 0.605
2.135SerMet: 2.135 ± 0.371
2.503SerAsn: 2.503 ± 0.501
2.43SerPro: 2.43 ± 0.427
2.871SerGln: 2.871 ± 0.405
3.092SerArg: 3.092 ± 0.529
3.313SerSer: 3.313 ± 0.629
3.018SerThr: 3.018 ± 0.391
3.681SerVal: 3.681 ± 0.585
0.736SerTrp: 0.736 ± 0.324
2.135SerTyr: 2.135 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
4.859ThrAla: 4.859 ± 0.462
0.442ThrCys: 0.442 ± 0.166
4.196ThrAsp: 4.196 ± 0.592
3.239ThrGlu: 3.239 ± 0.571
2.061ThrPhe: 2.061 ± 0.379
4.638ThrGly: 4.638 ± 0.522
1.693ThrHis: 1.693 ± 0.309
2.871ThrIle: 2.871 ± 0.353
4.123ThrLys: 4.123 ± 0.568
4.933ThrLeu: 4.933 ± 0.57
1.767ThrMet: 1.767 ± 0.3
2.356ThrAsn: 2.356 ± 0.456
2.577ThrPro: 2.577 ± 0.441
3.534ThrGln: 3.534 ± 0.637
1.914ThrArg: 1.914 ± 0.344
3.313ThrSer: 3.313 ± 0.656
4.565ThrThr: 4.565 ± 0.661
3.976ThrVal: 3.976 ± 0.566
0.515ThrTrp: 0.515 ± 0.22
2.65ThrTyr: 2.65 ± 0.531
0.0ThrXaa: 0.0 ± 0.0
Val
5.89ValAla: 5.89 ± 0.62
0.81ValCys: 0.81 ± 0.224
4.344ValAsp: 4.344 ± 0.668
3.755ValGlu: 3.755 ± 0.482
2.135ValPhe: 2.135 ± 0.347
4.712ValGly: 4.712 ± 0.675
1.178ValHis: 1.178 ± 0.302
3.681ValIle: 3.681 ± 0.499
4.344ValLys: 4.344 ± 0.632
5.595ValLeu: 5.595 ± 0.507
1.767ValMet: 1.767 ± 0.42
4.27ValAsn: 4.27 ± 0.559
2.798ValPro: 2.798 ± 0.408
2.43ValGln: 2.43 ± 0.413
3.387ValArg: 3.387 ± 0.543
4.491ValSer: 4.491 ± 0.594
4.859ValThr: 4.859 ± 0.672
4.344ValVal: 4.344 ± 0.606
0.81ValTrp: 0.81 ± 0.327
1.914ValTyr: 1.914 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
0.883TrpAla: 0.883 ± 0.271
0.221TrpCys: 0.221 ± 0.125
0.957TrpAsp: 0.957 ± 0.287
0.736TrpGlu: 0.736 ± 0.213
0.589TrpPhe: 0.589 ± 0.191
0.81TrpGly: 0.81 ± 0.249
0.515TrpHis: 0.515 ± 0.176
0.589TrpIle: 0.589 ± 0.192
0.883TrpLys: 0.883 ± 0.178
1.472TrpLeu: 1.472 ± 0.242
0.294TrpMet: 0.294 ± 0.181
0.663TrpAsn: 0.663 ± 0.194
0.589TrpPro: 0.589 ± 0.234
0.663TrpGln: 0.663 ± 0.203
0.663TrpArg: 0.663 ± 0.202
1.104TrpSer: 1.104 ± 0.296
0.663TrpThr: 0.663 ± 0.216
0.81TrpVal: 0.81 ± 0.259
0.147TrpTrp: 0.147 ± 0.093
0.442TrpTyr: 0.442 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.092TyrAla: 3.092 ± 0.39
0.442TyrCys: 0.442 ± 0.14
2.65TyrAsp: 2.65 ± 0.452
1.767TyrGlu: 1.767 ± 0.294
1.62TyrPhe: 1.62 ± 0.313
3.313TyrGly: 3.313 ± 0.525
0.81TyrHis: 0.81 ± 0.271
2.503TyrIle: 2.503 ± 0.545
2.43TyrLys: 2.43 ± 0.456
2.871TyrLeu: 2.871 ± 0.48
0.957TyrMet: 0.957 ± 0.262
1.988TyrAsn: 1.988 ± 0.374
1.546TyrPro: 1.546 ± 0.332
1.546TyrGln: 1.546 ± 0.277
1.841TyrArg: 1.841 ± 0.302
3.018TyrSer: 3.018 ± 0.497
1.988TyrThr: 1.988 ± 0.306
2.577TyrVal: 2.577 ± 0.461
0.442TyrTrp: 0.442 ± 0.157
1.325TyrTyr: 1.325 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13584 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski