Corynespora cassiicola Philippines
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17125 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T2N800|A0A2T2N800_CORCC Short chain dehydrogenase OS=Corynespora cassiicola Philippines OX=1448308 GN=BS50DRAFT_651787 PE=4 SV=1
MM1 pKa = 7.57 GGFSRR6 pKa = 11.84 ISALLLAAGYY16 pKa = 10.04 LGSEE20 pKa = 4.44 VAAAAVPAVPVPSAPQTDD38 pKa = 3.68 RR39 pKa = 11.84 VLRR42 pKa = 11.84 AATRR46 pKa = 11.84 RR47 pKa = 11.84 SNLHH51 pKa = 5.55 RR52 pKa = 11.84 RR53 pKa = 11.84 GLRR56 pKa = 11.84 IEE58 pKa = 4.24 KK59 pKa = 10.52 KK60 pKa = 10.19 FGSEE64 pKa = 3.23 LAYY67 pKa = 9.86 IEE69 pKa = 4.68 EE70 pKa = 4.49 EE71 pKa = 4.1 NGLSGRR77 pKa = 11.84 PVFASHH83 pKa = 5.28 VTVGSKK89 pKa = 10.54 KK90 pKa = 10.24 PILNLEE96 pKa = 4.3 EE97 pKa = 5.25 IEE99 pKa = 6.45 DD100 pKa = 3.8 YY101 pKa = 11.21 LQDD104 pKa = 3.79 VKK106 pKa = 11.13 CGSDD110 pKa = 3.04 GKK112 pKa = 10.3 MKK114 pKa = 10.74 LVFKK118 pKa = 10.78 DD119 pKa = 4.43 RR120 pKa = 11.84 IAARR124 pKa = 11.84 DD125 pKa = 3.56 ARR127 pKa = 11.84 LACSDD132 pKa = 3.92 EE133 pKa = 4.64 DD134 pKa = 3.62 GGLIITSHH142 pKa = 6.47 EE143 pKa = 4.13 GCNVDD148 pKa = 3.97 GEE150 pKa = 4.34 RR151 pKa = 11.84 SVYY154 pKa = 9.44 SVKK157 pKa = 10.74 DD158 pKa = 2.96 IAFADD163 pKa = 3.97 DD164 pKa = 3.91 EE165 pKa = 4.63 NALEE169 pKa = 4.03 LAVAEE174 pKa = 4.8 KK175 pKa = 8.96 MWKK178 pKa = 10.12 DD179 pKa = 3.36 AFDD182 pKa = 4.02 HH183 pKa = 6.5 FDD185 pKa = 2.87 ISFGHH190 pKa = 5.54 TTDD193 pKa = 2.96 KK194 pKa = 10.7 FHH196 pKa = 6.83 YY197 pKa = 7.51 RR198 pKa = 11.84 THH200 pKa = 7.13 RR201 pKa = 11.84 DD202 pKa = 3.16 FARR205 pKa = 11.84 RR206 pKa = 11.84 KK207 pKa = 8.07 RR208 pKa = 11.84 QEE210 pKa = 3.82 AGEE213 pKa = 4.12 PDD215 pKa = 3.59 ITPQPVEE222 pKa = 4.47 IPTDD226 pKa = 3.31 IPDD229 pKa = 4.08 DD230 pKa = 4.23 VNSVEE235 pKa = 6.32 FDD237 pKa = 3.79 LTWEE241 pKa = 4.7 KK242 pKa = 10.21 IDD244 pKa = 3.76 EE245 pKa = 4.37 VFSVSDD251 pKa = 3.6 FTAGLEE257 pKa = 4.09 QFVNVPTPADD267 pKa = 3.57 LPIEE271 pKa = 4.44 LGCTEE276 pKa = 5.49 CSTQGTITLSQGAFEE291 pKa = 4.79 IDD293 pKa = 3.41 VGEE296 pKa = 4.56 LDD298 pKa = 3.81 VVPDD302 pKa = 4.35 FLQGGDD308 pKa = 3.77 DD309 pKa = 4.02 GKK311 pKa = 10.72 DD312 pKa = 2.86 ISSVITGGFIEE323 pKa = 5.06 LKK325 pKa = 9.01 ANSVFGHH332 pKa = 6.6 FDD334 pKa = 3.41 LFARR338 pKa = 11.84 PRR340 pKa = 11.84 AAGSFEE346 pKa = 3.64 ITLFSLPVLGFTIPNIGRR364 pKa = 11.84 AGAVFEE370 pKa = 5.2 AGLSADD376 pKa = 3.64 YY377 pKa = 10.78 DD378 pKa = 4.05 VEE380 pKa = 6.01 GGLDD384 pKa = 3.65 LSYY387 pKa = 11.16 GIDD390 pKa = 3.36 VTVPDD395 pKa = 4.68 DD396 pKa = 3.47 ASMRR400 pKa = 11.84 IEE402 pKa = 4.98 LGDD405 pKa = 3.71 LKK407 pKa = 11.01 KK408 pKa = 10.99 SGVSGFAEE416 pKa = 4.39 STLEE420 pKa = 3.91 PLPFSANVTDD430 pKa = 4.14 VDD432 pKa = 4.0 ILLGLAFVPTIPIGFMFADD451 pKa = 3.95 KK452 pKa = 10.82 LDD454 pKa = 3.99 ASVTVSMDD462 pKa = 3.89 LPRR465 pKa = 11.84 LDD467 pKa = 4.87 ALITAKK473 pKa = 10.71 PNGDD477 pKa = 3.76 CKK479 pKa = 11.04 SILGGNSTSVASLGNSTNGDD499 pKa = 4.13 LSPVIIVDD507 pKa = 3.66 ANVSIVLDD515 pKa = 3.65 VGADD519 pKa = 3.59 FSFPILPIDD528 pKa = 4.68 PIGTAAEE535 pKa = 3.92 IFSTKK540 pKa = 10.19 FEE542 pKa = 5.01 LGTRR546 pKa = 11.84 CLAPGEE552 pKa = 4.48 GFAQATGAAAAGVGNQLPGFVLTAGYY578 pKa = 10.28 GDD580 pKa = 3.84 SASTCGGSTVTVYY593 pKa = 10.43 QPPPTGGAQYY603 pKa = 10.53 PPYY606 pKa = 9.71 EE607 pKa = 4.24 PSAPVIEE614 pKa = 4.98 PPVITPPPCSGYY626 pKa = 10.64 DD627 pKa = 3.54 CPDD630 pKa = 3.51 SPEE633 pKa = 4.66 SPPSPPVQSEE643 pKa = 4.35 PNCNGYY649 pKa = 9.61 EE650 pKa = 4.27 CPKK653 pKa = 10.13 PPSPPVEE660 pKa = 4.59 SNPPPPPAEE669 pKa = 4.36 SDD671 pKa = 3.64 PGCNGYY677 pKa = 10.11 DD678 pKa = 3.78 CPPPVAPPQSEE689 pKa = 4.42 PPSPPQSEE697 pKa = 4.28 PVSPPVEE704 pKa = 4.24 SDD706 pKa = 3.38 PGCNGYY712 pKa = 9.88 DD713 pKa = 3.94 CPQQPPVEE721 pKa = 4.4 PPIEE725 pKa = 4.39 PPVTTTPCDD734 pKa = 3.58 SSTLPPATEE743 pKa = 4.09 PPKK746 pKa = 10.33 TDD748 pKa = 3.33 SPPPEE753 pKa = 4.68 IPSAPVEE760 pKa = 4.44 PPVSTAPPSPPEE772 pKa = 4.15 QPSSPPEE779 pKa = 4.1 SPPEE783 pKa = 4.1 QPSSPPEE790 pKa = 4.09 NPPEE794 pKa = 4.25 QPSSPPEE801 pKa = 4.12 NPPVEE806 pKa = 4.4 PPKK809 pKa = 8.76 TTAPPPPPSTDD820 pKa = 3.16 VPEE823 pKa = 4.8 PPSTTVCDD831 pKa = 4.57 DD832 pKa = 3.69 YY833 pKa = 11.9 SCNEE837 pKa = 4.05 SPEE840 pKa = 4.28 NPPSQPAIPPMEE852 pKa = 4.67 PPSTTEE858 pKa = 3.66 SSEE861 pKa = 4.1 QPTPPPEE868 pKa = 4.39 NPPEE872 pKa = 4.27 VPSVPVEE879 pKa = 4.15 PPVSSSMVEE888 pKa = 4.16 PPPEE892 pKa = 4.66 APTPPPCDD900 pKa = 4.15 GYY902 pKa = 11.4 DD903 pKa = 3.46 CTEE906 pKa = 4.17 TPDD909 pKa = 3.83 VPSQPVEE916 pKa = 4.28 PPMSTPPPPPVDD928 pKa = 3.79 EE929 pKa = 4.78 PSVPAEE935 pKa = 4.31 PPVSPPSTGDD945 pKa = 3.15 IFPTPPPCDD954 pKa = 4.39 GYY956 pKa = 11.32 DD957 pKa = 3.44 CPEE960 pKa = 4.3 NPDD963 pKa = 3.76 VPSQPAEE970 pKa = 4.25 PPVSTPTPPPTVEE983 pKa = 4.3 EE984 pKa = 4.16 PSEE987 pKa = 4.05 PVNPPVSPPSTGDD1000 pKa = 3.27 IVPTPPPEE1008 pKa = 4.37 NPEE1011 pKa = 4.28 SPPEE1015 pKa = 3.98 QPPTTSAPEE1024 pKa = 3.86 CDD1026 pKa = 4.52 GYY1028 pKa = 11.58 GCEE1031 pKa = 5.1 PPTEE1035 pKa = 4.25 TPPPSGDD1042 pKa = 3.56 EE1043 pKa = 3.73 PSKK1046 pKa = 10.61 PIEE1049 pKa = 4.65 PPVSTPPTEE1058 pKa = 4.79 PPTEE1062 pKa = 4.21 TPPPCDD1068 pKa = 3.34 GDD1070 pKa = 4.38 CPQEE1074 pKa = 4.45 PPAEE1078 pKa = 4.51 PPVSTPVTEE1087 pKa = 5.1 PPTEE1091 pKa = 4.17 TPPPCDD1097 pKa = 3.46 GDD1099 pKa = 4.49 CPTEE1103 pKa = 4.52 PPTEE1107 pKa = 4.21 TPPPCDD1113 pKa = 5.01 GYY1115 pKa = 11.39 DD1116 pKa = 3.9 CNNSQPPTEE1125 pKa = 5.15 PPTEE1129 pKa = 4.09 TPPPGGYY1136 pKa = 9.76 EE1137 pKa = 3.73 PSKK1140 pKa = 9.94 PAEE1143 pKa = 4.6 PPVSTPPTEE1152 pKa = 4.79 PPTEE1156 pKa = 4.21 TPPPCDD1162 pKa = 3.46 GDD1164 pKa = 4.49 CPTEE1168 pKa = 4.52 PPTEE1172 pKa = 4.21 TPPPCDD1178 pKa = 4.67 GYY1180 pKa = 11.38 DD1181 pKa = 3.61 CPTEE1185 pKa = 4.07 SSPPCSGPDD1194 pKa = 3.57 CPSEE1198 pKa = 4.37 PPVQTPPPSAEE1209 pKa = 4.37 TPCTTSSTQTITLSTGGTGGGGYY1232 pKa = 9.81 PNTTAGGYY1240 pKa = 9.5 GHH1242 pKa = 7.26 TPPSYY1247 pKa = 10.29 PPATTMVEE1255 pKa = 4.12 VPPPYY1260 pKa = 10.21 GGYY1263 pKa = 9.43 PPPASHH1269 pKa = 6.88 TGNMTMQTGFLDD1281 pKa = 4.59 GPTGTRR1287 pKa = 11.84 PPMQTTSPAQFTGAAVPRR1305 pKa = 11.84 AVPIAVDD1312 pKa = 3.54 AKK1314 pKa = 10.16 GLGWQGAVLVMSLFAGGFMLLL1335 pKa = 3.88
Molecular weight: 138.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.948
Patrickios 0.807
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.841
Protein with the highest isoelectric point:
>tr|A0A2T2NI65|A0A2T2NI65_CORCC Uncharacterized protein OS=Corynespora cassiicola Philippines OX=1448308 GN=BS50DRAFT_51737 PE=4 SV=1
MM1 pKa = 7.34 AVAVAAGGGLTAALRR16 pKa = 11.84 PPSKK20 pKa = 10.37 PSPTTTASRR29 pKa = 11.84 ASQALRR35 pKa = 11.84 LPSARR40 pKa = 11.84 CPLPSALSQPSRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 ASGASPATAPPLPTASIKK72 pKa = 9.8 PANGPSALAPSRR84 pKa = 11.84 PRR86 pKa = 11.84 RR87 pKa = 11.84 AARR90 pKa = 11.84 LAMAARR96 pKa = 11.84 APPPTAARR104 pKa = 11.84 HH105 pKa = 5.13 PPSAIRR111 pKa = 11.84 HH112 pKa = 5.38 PPSAIHH118 pKa = 6.74 RR119 pKa = 11.84 PSQTRR124 pKa = 11.84 LPSQSRR130 pKa = 11.84 LGG132 pKa = 3.55
Molecular weight: 13.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.496
IPC2_protein 11.111
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.427
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.149
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17125
0
17125
7195570
49
7227
420.2
46.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.65 ± 0.017
1.378 ± 0.008
5.468 ± 0.014
6.055 ± 0.018
3.855 ± 0.012
6.917 ± 0.018
2.499 ± 0.009
4.957 ± 0.014
4.855 ± 0.016
8.829 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.202 ± 0.007
3.706 ± 0.01
6.21 ± 0.026
3.923 ± 0.013
6.157 ± 0.017
8.078 ± 0.019
5.817 ± 0.013
6.018 ± 0.013
1.594 ± 0.007
2.834 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here