Clostridium isatidis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343J928|A0A343J928_9CLOT Fatty acid-binding protein DegV OS=Clostridium isatidis OX=182773 GN=BEN51_00480 PE=4 SV=1
MM1 pKa = 7.27IAKK4 pKa = 9.48VDD6 pKa = 3.4QDD8 pKa = 3.77TCIGCGLCPTICEE21 pKa = 4.7DD22 pKa = 3.46VFEE25 pKa = 5.0MGDD28 pKa = 3.27EE29 pKa = 4.32GKK31 pKa = 10.38AQVKK35 pKa = 10.1VDD37 pKa = 3.6VVPEE41 pKa = 4.2GSEE44 pKa = 4.03DD45 pKa = 3.4AAKK48 pKa = 10.05EE49 pKa = 4.09AEE51 pKa = 4.38EE52 pKa = 4.28SCPVNAIEE60 pKa = 5.22VEE62 pKa = 4.16

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343J9H7|A0A343J9H7_9CLOT Energy-coupling factor transporter ATP-binding protein EcfA OS=Clostridium isatidis OX=182773 GN=ecfA PE=3 SV=1
MM1 pKa = 7.69LMPKK5 pKa = 9.8RR6 pKa = 11.84VKK8 pKa = 9.77RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 7.27VQRR14 pKa = 11.84GRR16 pKa = 11.84MKK18 pKa = 10.87GKK20 pKa = 8.57ATRR23 pKa = 11.84GNFLAYY29 pKa = 10.3GDD31 pKa = 4.53FGIQAMEE38 pKa = 4.52CGWIRR43 pKa = 11.84SNQIEE48 pKa = 4.48SARR51 pKa = 11.84IAINRR56 pKa = 11.84YY57 pKa = 7.12IRR59 pKa = 11.84RR60 pKa = 11.84GGKK63 pKa = 9.08LWIKK67 pKa = 10.17IFPDD71 pKa = 3.28KK72 pKa = 10.85PVTQKK77 pKa = 11.31AAGTRR82 pKa = 11.84MGSGKK87 pKa = 10.56GNPEE91 pKa = 3.42YY92 pKa = 9.89WVAVVKK98 pKa = 10.21PGRR101 pKa = 11.84VLFEE105 pKa = 3.91LSGVNEE111 pKa = 4.1EE112 pKa = 4.41VARR115 pKa = 11.84EE116 pKa = 4.02AMRR119 pKa = 11.84LASHH123 pKa = 7.33KK124 pKa = 10.85LPVKK128 pKa = 9.5TKK130 pKa = 9.39FVTRR134 pKa = 11.84RR135 pKa = 11.84DD136 pKa = 3.67FEE138 pKa = 4.42EE139 pKa = 4.17MGGEE143 pKa = 4.08EE144 pKa = 3.99

Molecular weight: 16.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2554

0

2554

799527

37

2824

313.0

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.74 ± 0.054

0.991 ± 0.016

5.405 ± 0.038

8.164 ± 0.062

4.371 ± 0.042

6.223 ± 0.045

1.221 ± 0.016

10.519 ± 0.06

9.318 ± 0.046

9.467 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.023

6.491 ± 0.047

2.663 ± 0.022

2.047 ± 0.023

3.469 ± 0.033

6.014 ± 0.041

4.568 ± 0.031

6.095 ± 0.038

0.612 ± 0.014

4.244 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski