Lynx canadensis faeces associated genomovirus CL1 46
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ18|A0A2Z5CJ18_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL1 46 OX=2219124 PE=4 SV=1
MM1 pKa = 7.73 PFVCNARR8 pKa = 11.84 YY9 pKa = 8.99 FLVTYY14 pKa = 9.3 SHH16 pKa = 7.29 VEE18 pKa = 3.84 EE19 pKa = 5.21 LDD21 pKa = 3.37 PFALVEE27 pKa = 4.18 FFGNLGAEE35 pKa = 4.51 CIVGLEE41 pKa = 4.35 PYY43 pKa = 10.0 NATFGIHH50 pKa = 5.5 FHH52 pKa = 6.15 VFTDD56 pKa = 3.97 FGRR59 pKa = 11.84 KK60 pKa = 8.01 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 TDD67 pKa = 2.75 IFDD70 pKa = 3.55 VNGFHH75 pKa = 7.44 PNISPSRR82 pKa = 11.84 GTPEE86 pKa = 3.98 AGYY89 pKa = 10.67 DD90 pKa = 3.63 YY91 pKa = 10.72 AIKK94 pKa = 10.92 NGDD97 pKa = 3.51 VVAGGLARR105 pKa = 11.84 PSGVGNSGRR114 pKa = 11.84 AAKK117 pKa = 8.7 WHH119 pKa = 5.93 QIVDD123 pKa = 3.26 AEE125 pKa = 4.35 TRR127 pKa = 11.84 DD128 pKa = 3.72 EE129 pKa = 5.46 FFDD132 pKa = 3.82 LCEE135 pKa = 4.1 EE136 pKa = 4.75 LDD138 pKa = 3.9 PEE140 pKa = 4.33 RR141 pKa = 11.84 LVCSFSQIQKK151 pKa = 8.17 YY152 pKa = 10.48 ADD154 pKa = 2.77 WRR156 pKa = 11.84 FAEE159 pKa = 4.19 NPEE162 pKa = 4.71 PYY164 pKa = 10.23 ASPDD168 pKa = 3.39 GVFDD172 pKa = 4.25 LANYY176 pKa = 9.84 GDD178 pKa = 4.11 LGEE181 pKa = 4.52 SKK183 pKa = 10.79 SLILWGPSRR192 pKa = 11.84 MGKK195 pKa = 6.86 TVWARR200 pKa = 11.84 SLGDD204 pKa = 3.33 HH205 pKa = 7.09 LYY207 pKa = 10.87 FGGIFSARR215 pKa = 11.84 DD216 pKa = 3.12 IGRR219 pKa = 11.84 GGIKK223 pKa = 10.28 YY224 pKa = 10.52 AVFDD228 pKa = 5.44 DD229 pKa = 3.44 IAGGIKK235 pKa = 10.03 FFPRR239 pKa = 11.84 FKK241 pKa = 10.79 DD242 pKa = 3.12 WLGCQMEE249 pKa = 4.36 FMVKK253 pKa = 9.32 EE254 pKa = 4.5 MYY256 pKa = 10.19 RR257 pKa = 11.84 DD258 pKa = 3.27 PHH260 pKa = 6.35 LFKK263 pKa = 10.36 WGRR266 pKa = 11.84 PAIWIANTDD275 pKa = 3.42 PRR277 pKa = 11.84 HH278 pKa = 6.13 DD279 pKa = 3.82 MSHH282 pKa = 6.98 EE283 pKa = 4.0 DD284 pKa = 3.94 VTWLEE289 pKa = 4.04 ANCIFVEE296 pKa = 3.75 IDD298 pKa = 3.19 SAIFHH303 pKa = 7.06 ANTEE307 pKa = 4.17
Molecular weight: 34.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.982
IPC2_protein 5.029
IPC_protein 4.991
Toseland 4.965
ProMoST 5.092
Dawson 5.016
Bjellqvist 5.156
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.902
Solomon 5.016
Lehninger 4.978
Nozaki 5.143
DTASelect 5.334
Thurlkill 4.978
EMBOSS 4.965
Sillero 5.219
Patrickios 3.859
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.166
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ18|A0A2Z5CJ18_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL1 46 OX=2219124 PE=4 SV=1
MM1 pKa = 8.11 AYY3 pKa = 10.07 RR4 pKa = 11.84 GRR6 pKa = 11.84 GPVRR10 pKa = 11.84 RR11 pKa = 11.84 NFRR14 pKa = 11.84 AAAKK18 pKa = 9.63 KK19 pKa = 10.16 RR20 pKa = 11.84 YY21 pKa = 9.46 AGRR24 pKa = 11.84 SKK26 pKa = 10.43 FRR28 pKa = 11.84 RR29 pKa = 11.84 SYY31 pKa = 9.45 GKK33 pKa = 9.93 KK34 pKa = 9.04 RR35 pKa = 11.84 IYY37 pKa = 10.15 RR38 pKa = 11.84 KK39 pKa = 9.62 RR40 pKa = 11.84 AVPTKK45 pKa = 10.33 RR46 pKa = 11.84 ILNLTSRR53 pKa = 11.84 KK54 pKa = 9.72 KK55 pKa = 9.8 QDD57 pKa = 2.99 NMLSFSTTSASGANQSPGVGGLYY80 pKa = 10.64 VNGSAASAMSVFCPTARR97 pKa = 11.84 SLVSAGTTNLTVDD110 pKa = 3.2 VSDD113 pKa = 4.25 RR114 pKa = 11.84 TSSTCFMRR122 pKa = 11.84 GYY124 pKa = 10.33 RR125 pKa = 11.84 EE126 pKa = 3.76 NLRR129 pKa = 11.84 IQTSSPLPWLWRR141 pKa = 11.84 RR142 pKa = 11.84 IVFTSKK148 pKa = 10.45 GGTPFGSQLASDD160 pKa = 3.52 QSQYY164 pKa = 11.14 VKK166 pKa = 10.54 YY167 pKa = 10.56 APYY170 pKa = 10.73 SDD172 pKa = 3.65 TTIGMARR179 pKa = 11.84 LWFNLSNNNTPNTLASIQSVLFRR202 pKa = 11.84 GTINQDD208 pKa = 2.63 WNDD211 pKa = 3.62 VITAKK216 pKa = 10.43 VDD218 pKa = 3.43 TSRR221 pKa = 11.84 VTVMSDD227 pKa = 2.17 KK228 pKa = 11.11 TMTIRR233 pKa = 11.84 TGNANGHH240 pKa = 4.96 FSEE243 pKa = 4.55 RR244 pKa = 11.84 KK245 pKa = 8.83 LWYY248 pKa = 9.57 PMNKK252 pKa = 9.98 NIVYY256 pKa = 10.6 DD257 pKa = 4.13 DD258 pKa = 4.79 DD259 pKa = 4.37 EE260 pKa = 6.55 SGAAMTTAYY269 pKa = 10.58 SSTEE273 pKa = 3.9 AKK275 pKa = 10.29 PGMGDD280 pKa = 3.2 MYY282 pKa = 11.28 VADD285 pKa = 4.51 FLVPGIGGTASDD297 pKa = 5.04 IISLNCTATLYY308 pKa = 9.4 WHH310 pKa = 7.01 EE311 pKa = 4.29 KK312 pKa = 9.3
Molecular weight: 34.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.706
IPC_protein 10.204
Toseland 10.292
ProMoST 10.043
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.804
Grimsley 10.57
Solomon 10.526
Lehninger 10.482
Nozaki 10.277
DTASelect 10.175
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.394
Patrickios 10.394
IPC_peptide 10.526
IPC2_peptide 9.048
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
619
307
312
309.5
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.078 ± 0.18
1.454 ± 0.347
6.3 ± 1.053
4.039 ± 1.719
6.139 ± 1.844
8.562 ± 0.614
1.939 ± 0.916
5.008 ± 0.594
4.523 ± 0.653
5.816 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.55
5.008 ± 0.763
4.523 ± 0.478
1.939 ± 0.441
7.27 ± 0.75
8.078 ± 1.99
6.462 ± 2.225
5.816 ± 0.033
2.262 ± 0.239
4.039 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here