Mycoplasma struthionis
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 590 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8LIM2|A0A3G8LIM2_9MOLU 50S ribosomal protein L3 OS=Mycoplasma struthionis OX=538220 GN=rplC PE=3 SV=1
MM1 pKa = 7.63 FEE3 pKa = 4.36 EE4 pKa = 4.66 PLCEE8 pKa = 4.09 VGLLAVPVSPAGAEE22 pKa = 4.13 LEE24 pKa = 4.25 PDD26 pKa = 3.82 SACFPAEE33 pKa = 4.12 EE34 pKa = 4.51 GVVAADD40 pKa = 3.12 SGEE43 pKa = 4.52 GYY45 pKa = 10.31 FSSEE49 pKa = 4.25 EE50 pKa = 3.79 EE51 pKa = 4.36 VVAVGSFNSNFIVNDD66 pKa = 3.65 PSEE69 pKa = 4.42 INGAFPLPLNWANLAVIVIYY89 pKa = 10.42 GFAFSVFAVKK99 pKa = 10.04 VV100 pKa = 3.49
Molecular weight: 10.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.238
IPC2_protein 3.452
IPC_protein 3.236
Toseland 3.134
ProMoST 3.439
Dawson 3.236
Bjellqvist 3.414
Wikipedia 3.16
Rodwell 3.121
Grimsley 3.071
Solomon 3.121
Lehninger 3.071
Nozaki 3.401
DTASelect 3.389
Thurlkill 3.198
EMBOSS 3.172
Sillero 3.376
Patrickios 0.006
IPC_peptide 3.121
IPC2_peptide 3.325
IPC2.peptide.svr19 3.618
Protein with the highest isoelectric point:
>tr|A0A3G8LGF7|A0A3G8LGF7_9MOLU 30S ribosomal protein S3 OS=Mycoplasma struthionis OX=538220 GN=rpsC PE=3 SV=1
MM1 pKa = 7.33 ARR3 pKa = 11.84 KK4 pKa = 9.63 ALMVKK9 pKa = 10.4 AEE11 pKa = 4.12 RR12 pKa = 11.84 QPKK15 pKa = 9.69 FGVRR19 pKa = 11.84 KK20 pKa = 7.38 YY21 pKa = 8.64 TRR23 pKa = 11.84 CQLCGRR29 pKa = 11.84 VHH31 pKa = 6.88 AVLRR35 pKa = 11.84 KK36 pKa = 9.58 YY37 pKa = 10.23 KK38 pKa = 9.85 ICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 3.88 LAHH49 pKa = 6.54 EE50 pKa = 4.41 GKK52 pKa = 10.21 IPGVKK57 pKa = 9.38 KK58 pKa = 10.89 ASWW61 pKa = 3.03
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.765
IPC_protein 10.248
Toseland 10.877
ProMoST 10.452
Dawson 10.95
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.359
Grimsley 10.965
Solomon 11.038
Lehninger 11.023
Nozaki 10.862
DTASelect 10.584
Thurlkill 10.862
EMBOSS 11.257
Sillero 10.877
Patrickios 11.096
IPC_peptide 11.052
IPC2_peptide 9.706
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
590
0
590
206476
37
3078
350.0
40.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.009 ± 0.116
0.441 ± 0.027
5.653 ± 0.081
7.166 ± 0.114
5.168 ± 0.108
4.489 ± 0.111
1.302 ± 0.036
8.751 ± 0.131
10.523 ± 0.114
9.819 ± 0.104
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.699 ± 0.053
8.125 ± 0.143
2.783 ± 0.063
3.208 ± 0.078
3.01 ± 0.064
6.459 ± 0.076
5.042 ± 0.076
5.373 ± 0.083
0.837 ± 0.032
4.141 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here