Sphingomonas sp. ABOLF

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430DR42|A0A430DR42_9SPHN Endonuclease OS=Sphingomonas sp. ABOLF OX=1985879 GN=CA235_05890 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 6.48IIRR5 pKa = 11.84LPSGEE10 pKa = 4.29VAPGDD15 pKa = 3.6VDD17 pKa = 4.41CISIVALSDD26 pKa = 3.07GRR28 pKa = 11.84YY29 pKa = 9.4DD30 pKa = 3.63LSGAALVACGDD41 pKa = 3.62GDD43 pKa = 4.06EE44 pKa = 4.47VQSVALIGLPPFEE57 pKa = 4.26TRR59 pKa = 11.84EE60 pKa = 4.01AAEE63 pKa = 4.27DD64 pKa = 4.09AGLAWAAEE72 pKa = 4.09QCVEE76 pKa = 4.61RR77 pKa = 11.84IYY79 pKa = 10.35VTDD82 pKa = 3.64VEE84 pKa = 5.24FCVQPP89 pKa = 3.72

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430DLG4|A0A430DLG4_9SPHN DMT family transporter OS=Sphingomonas sp. ABOLF OX=1985879 GN=CA235_11715 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.4GFRR19 pKa = 11.84TRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.2VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3641

0

3641

1165691

26

2353

320.2

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.816 ± 0.057

0.728 ± 0.012

5.834 ± 0.032

5.552 ± 0.036

3.423 ± 0.023

8.938 ± 0.039

1.92 ± 0.021

4.457 ± 0.023

2.641 ± 0.031

9.992 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.021

2.405 ± 0.028

5.545 ± 0.033

3.203 ± 0.024

7.89 ± 0.044

5.009 ± 0.031

5.409 ± 0.03

7.395 ± 0.033

1.461 ± 0.018

2.199 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski