Amino acid dipepetide frequency for Sphingomonas sp. ABOLF

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.722AlaAla: 20.722 ± 0.223
1.079AlaCys: 1.079 ± 0.038
7.827AlaAsp: 7.827 ± 0.157
8.533AlaGlu: 8.533 ± 0.122
4.352AlaPhe: 4.352 ± 0.063
12.27AlaGly: 12.27 ± 0.116
2.321AlaHis: 2.321 ± 0.052
6.519AlaIle: 6.519 ± 0.076
3.761AlaLys: 3.761 ± 0.072
14.317AlaLeu: 14.317 ± 0.139
3.725AlaMet: 3.725 ± 0.062
3.039AlaAsn: 3.039 ± 0.065
7.079AlaPro: 7.079 ± 0.108
4.743AlaGln: 4.743 ± 0.074
10.493AlaArg: 10.493 ± 0.117
6.678AlaSer: 6.678 ± 0.089
7.164AlaThr: 7.164 ± 0.096
9.201AlaVal: 9.201 ± 0.123
1.783AlaTrp: 1.783 ± 0.045
2.554AlaTyr: 2.554 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.951CysAla: 0.951 ± 0.033
0.083CysCys: 0.083 ± 0.008
0.498CysAsp: 0.498 ± 0.022
0.349CysGlu: 0.349 ± 0.016
0.253CysPhe: 0.253 ± 0.014
0.794CysGly: 0.794 ± 0.029
0.201CysHis: 0.201 ± 0.012
0.28CysIle: 0.28 ± 0.017
0.123CysLys: 0.123 ± 0.011
0.631CysLeu: 0.631 ± 0.023
0.128CysMet: 0.128 ± 0.01
0.164CysAsn: 0.164 ± 0.013
0.448CysPro: 0.448 ± 0.021
0.187CysGln: 0.187 ± 0.011
0.581CysArg: 0.581 ± 0.023
0.431CysSer: 0.431 ± 0.022
0.394CysThr: 0.394 ± 0.021
0.528CysVal: 0.528 ± 0.023
0.106CysTrp: 0.106 ± 0.01
0.152CysTyr: 0.152 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.529AspAla: 8.529 ± 0.168
0.419AspCys: 0.419 ± 0.021
3.01AspAsp: 3.01 ± 0.062
3.111AspGlu: 3.111 ± 0.054
1.996AspPhe: 1.996 ± 0.041
5.499AspGly: 5.499 ± 0.085
1.167AspHis: 1.167 ± 0.036
2.327AspIle: 2.327 ± 0.039
1.385AspLys: 1.385 ± 0.035
5.642AspLeu: 5.642 ± 0.073
1.216AspMet: 1.216 ± 0.036
1.176AspAsn: 1.176 ± 0.033
3.999AspPro: 3.999 ± 0.066
1.842AspGln: 1.842 ± 0.043
5.265AspArg: 5.265 ± 0.074
2.189AspSer: 2.189 ± 0.046
2.82AspThr: 2.82 ± 0.048
4.184AspVal: 4.184 ± 0.065
1.095AspTrp: 1.095 ± 0.033
1.466AspTyr: 1.466 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
8.529GluAla: 8.529 ± 0.112
0.288GluCys: 0.288 ± 0.017
2.826GluAsp: 2.826 ± 0.062
3.026GluGlu: 3.026 ± 0.066
1.391GluPhe: 1.391 ± 0.045
4.804GluGly: 4.804 ± 0.073
1.178GluHis: 1.178 ± 0.031
2.591GluIle: 2.591 ± 0.049
1.668GluLys: 1.668 ± 0.043
5.059GluLeu: 5.059 ± 0.072
1.2GluMet: 1.2 ± 0.034
1.276GluAsn: 1.276 ± 0.033
2.768GluPro: 2.768 ± 0.054
2.322GluGln: 2.322 ± 0.045
5.604GluArg: 5.604 ± 0.078
2.099GluSer: 2.099 ± 0.046
3.158GluThr: 3.158 ± 0.052
3.975GluVal: 3.975 ± 0.064
0.793GluTrp: 0.793 ± 0.025
0.969GluTyr: 0.969 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.686PheAla: 4.686 ± 0.071
0.281PheCys: 0.281 ± 0.015
2.573PheAsp: 2.573 ± 0.051
2.039PheGlu: 2.039 ± 0.041
1.204PhePhe: 1.204 ± 0.039
3.601PheGly: 3.601 ± 0.063
0.699PheHis: 0.699 ± 0.022
1.084PheIle: 1.084 ± 0.031
0.733PheLys: 0.733 ± 0.027
3.106PheLeu: 3.106 ± 0.055
0.604PheMet: 0.604 ± 0.021
0.991PheAsn: 0.991 ± 0.032
1.488PhePro: 1.488 ± 0.031
0.945PheGln: 0.945 ± 0.033
2.35PheArg: 2.35 ± 0.052
1.863PheSer: 1.863 ± 0.042
1.93PheThr: 1.93 ± 0.046
2.619PheVal: 2.619 ± 0.054
0.534PheTrp: 0.534 ± 0.022
0.897PheTyr: 0.897 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
10.408GlyAla: 10.408 ± 0.097
0.818GlyCys: 0.818 ± 0.031
4.925GlyAsp: 4.925 ± 0.062
5.03GlyGlu: 5.03 ± 0.067
3.603GlyPhe: 3.603 ± 0.065
8.158GlyGly: 8.158 ± 0.107
1.777GlyHis: 1.777 ± 0.047
4.211GlyIle: 4.211 ± 0.054
2.953GlyLys: 2.953 ± 0.063
8.447GlyLeu: 8.447 ± 0.094
2.232GlyMet: 2.232 ± 0.051
2.17GlyAsn: 2.17 ± 0.049
3.622GlyPro: 3.622 ± 0.057
2.942GlyGln: 2.942 ± 0.057
7.079GlyArg: 7.079 ± 0.091
5.055GlySer: 5.055 ± 0.078
5.319GlyThr: 5.319 ± 0.082
6.565GlyVal: 6.565 ± 0.084
1.726GlyTrp: 1.726 ± 0.042
2.339GlyTyr: 2.339 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.53HisAla: 2.53 ± 0.058
0.186HisCys: 0.186 ± 0.013
1.184HisAsp: 1.184 ± 0.035
0.948HisGlu: 0.948 ± 0.032
0.721HisPhe: 0.721 ± 0.025
1.962HisGly: 1.962 ± 0.049
0.558HisHis: 0.558 ± 0.023
0.734HisIle: 0.734 ± 0.028
0.392HisLys: 0.392 ± 0.017
1.868HisLeu: 1.868 ± 0.043
0.413HisMet: 0.413 ± 0.021
0.419HisAsn: 0.419 ± 0.018
1.256HisPro: 1.256 ± 0.032
0.615HisGln: 0.615 ± 0.023
1.566HisArg: 1.566 ± 0.038
0.829HisSer: 0.829 ± 0.031
0.729HisThr: 0.729 ± 0.024
1.392HisVal: 1.392 ± 0.036
0.362HisTrp: 0.362 ± 0.016
0.533HisTyr: 0.533 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
7.253IleAla: 7.253 ± 0.088
0.314IleCys: 0.314 ± 0.017
3.56IleAsp: 3.56 ± 0.049
3.258IleGlu: 3.258 ± 0.055
1.222IlePhe: 1.222 ± 0.037
4.629IleGly: 4.629 ± 0.073
0.782IleHis: 0.782 ± 0.027
1.447IleIle: 1.447 ± 0.039
0.993IleLys: 0.993 ± 0.032
3.618IleLeu: 3.618 ± 0.059
0.612IleMet: 0.612 ± 0.023
1.188IleAsn: 1.188 ± 0.033
2.035IlePro: 2.035 ± 0.046
1.117IleGln: 1.117 ± 0.034
3.104IleArg: 3.104 ± 0.055
2.053IleSer: 2.053 ± 0.042
2.356IleThr: 2.356 ± 0.048
3.64IleVal: 3.64 ± 0.07
0.48IleTrp: 0.48 ± 0.024
0.915IleTyr: 0.915 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.506LysAla: 3.506 ± 0.071
0.125LysCys: 0.125 ± 0.01
1.295LysAsp: 1.295 ± 0.038
1.129LysGlu: 1.129 ± 0.034
0.684LysPhe: 0.684 ± 0.025
2.336LysGly: 2.336 ± 0.047
0.516LysHis: 0.516 ± 0.024
1.17LysIle: 1.17 ± 0.031
0.867LysLys: 0.867 ± 0.037
3.016LysLeu: 3.016 ± 0.054
0.599LysMet: 0.599 ± 0.025
0.639LysAsn: 0.639 ± 0.023
1.947LysPro: 1.947 ± 0.049
0.84LysGln: 0.84 ± 0.028
2.254LysArg: 2.254 ± 0.05
1.283LysSer: 1.283 ± 0.036
1.482LysThr: 1.482 ± 0.038
1.918LysVal: 1.918 ± 0.048
0.317LysTrp: 0.317 ± 0.016
0.489LysTyr: 0.489 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.656LeuAla: 14.656 ± 0.147
0.737LeuCys: 0.737 ± 0.027
6.262LeuAsp: 6.262 ± 0.079
4.973LeuGlu: 4.973 ± 0.071
3.54LeuPhe: 3.54 ± 0.069
8.559LeuGly: 8.559 ± 0.089
1.782LeuHis: 1.782 ± 0.042
4.159LeuIle: 4.159 ± 0.06
2.711LeuLys: 2.711 ± 0.052
9.991LeuLeu: 9.991 ± 0.139
1.917LeuMet: 1.917 ± 0.044
2.252LeuAsn: 2.252 ± 0.053
5.963LeuPro: 5.963 ± 0.072
2.68LeuGln: 2.68 ± 0.045
7.378LeuArg: 7.378 ± 0.101
5.785LeuSer: 5.785 ± 0.074
5.466LeuThr: 5.466 ± 0.079
7.754LeuVal: 7.754 ± 0.09
1.341LeuTrp: 1.341 ± 0.033
2.018LeuTyr: 2.018 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.992MetAla: 2.992 ± 0.056
0.109MetCys: 0.109 ± 0.01
0.949MetAsp: 0.949 ± 0.03
0.908MetGlu: 0.908 ± 0.031
0.613MetPhe: 0.613 ± 0.021
1.677MetGly: 1.677 ± 0.047
0.368MetHis: 0.368 ± 0.017
1.121MetIle: 1.121 ± 0.031
0.787MetLys: 0.787 ± 0.027
2.465MetLeu: 2.465 ± 0.057
0.541MetMet: 0.541 ± 0.023
0.572MetAsn: 0.572 ± 0.023
1.408MetPro: 1.408 ± 0.04
0.694MetGln: 0.694 ± 0.026
1.82MetArg: 1.82 ± 0.045
1.252MetSer: 1.252 ± 0.035
1.537MetThr: 1.537 ± 0.037
1.573MetVal: 1.573 ± 0.039
0.202MetTrp: 0.202 ± 0.014
0.238MetTyr: 0.238 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.106AsnAla: 3.106 ± 0.058
0.201AsnCys: 0.201 ± 0.012
1.323AsnAsp: 1.323 ± 0.032
1.111AsnGlu: 1.111 ± 0.035
0.88AsnPhe: 0.88 ± 0.034
2.428AsnGly: 2.428 ± 0.047
0.466AsnHis: 0.466 ± 0.02
1.096AsnIle: 1.096 ± 0.036
0.569AsnLys: 0.569 ± 0.026
2.455AsnLeu: 2.455 ± 0.049
0.435AsnMet: 0.435 ± 0.023
0.652AsnAsn: 0.652 ± 0.031
1.693AsnPro: 1.693 ± 0.042
0.748AsnGln: 0.748 ± 0.027
1.791AsnArg: 1.791 ± 0.039
1.104AsnSer: 1.104 ± 0.036
1.18AsnThr: 1.18 ± 0.035
1.773AsnVal: 1.773 ± 0.047
0.403AsnTrp: 0.403 ± 0.021
0.637AsnTyr: 0.637 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
7.74ProAla: 7.74 ± 0.099
0.304ProCys: 0.304 ± 0.017
3.765ProAsp: 3.765 ± 0.056
3.829ProGlu: 3.829 ± 0.053
1.958ProPhe: 1.958 ± 0.042
5.012ProGly: 5.012 ± 0.065
1.042ProHis: 1.042 ± 0.029
2.443ProIle: 2.443 ± 0.05
1.496ProLys: 1.496 ± 0.04
5.068ProLeu: 5.068 ± 0.073
1.174ProMet: 1.174 ± 0.033
1.399ProAsn: 1.399 ± 0.033
2.955ProPro: 2.955 ± 0.074
1.844ProGln: 1.844 ± 0.039
3.452ProArg: 3.452 ± 0.063
2.814ProSer: 2.814 ± 0.05
2.823ProThr: 2.823 ± 0.054
4.513ProVal: 4.513 ± 0.076
0.703ProTrp: 0.703 ± 0.025
1.117ProTyr: 1.117 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.609GlnAla: 4.609 ± 0.076
0.201GlnCys: 0.201 ± 0.015
1.433GlnAsp: 1.433 ± 0.034
1.439GlnGlu: 1.439 ± 0.038
1.075GlnPhe: 1.075 ± 0.029
2.585GlnGly: 2.585 ± 0.047
0.618GlnHis: 0.618 ± 0.029
1.492GlnIle: 1.492 ± 0.039
0.776GlnLys: 0.776 ± 0.028
3.303GlnLeu: 3.303 ± 0.063
0.782GlnMet: 0.782 ± 0.026
0.721GlnAsn: 0.721 ± 0.027
2.027GlnPro: 2.027 ± 0.042
1.29GlnGln: 1.29 ± 0.036
2.823GlnArg: 2.823 ± 0.05
1.645GlnSer: 1.645 ± 0.042
1.633GlnThr: 1.633 ± 0.038
2.535GlnVal: 2.535 ± 0.04
0.436GlnTrp: 0.436 ± 0.019
0.611GlnTyr: 0.611 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
9.853ArgAla: 9.853 ± 0.111
0.541ArgCys: 0.541 ± 0.023
4.399ArgAsp: 4.399 ± 0.063
4.407ArgGlu: 4.407 ± 0.064
3.409ArgPhe: 3.409 ± 0.066
5.468ArgGly: 5.468 ± 0.078
1.738ArgHis: 1.738 ± 0.041
4.064ArgIle: 4.064 ± 0.066
1.896ArgLys: 1.896 ± 0.041
8.722ArgLeu: 8.722 ± 0.117
1.962ArgMet: 1.962 ± 0.044
1.898ArgAsn: 1.898 ± 0.042
4.162ArgPro: 4.162 ± 0.065
2.657ArgGln: 2.657 ± 0.042
6.563ArgArg: 6.563 ± 0.095
3.958ArgSer: 3.958 ± 0.056
4.097ArgThr: 4.097 ± 0.066
5.521ArgVal: 5.521 ± 0.073
1.453ArgTrp: 1.453 ± 0.04
2.132ArgTyr: 2.132 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
6.219SerAla: 6.219 ± 0.071
0.4SerCys: 0.4 ± 0.019
2.896SerAsp: 2.896 ± 0.048
2.486SerGlu: 2.486 ± 0.047
2.073SerPhe: 2.073 ± 0.045
5.323SerGly: 5.323 ± 0.079
0.902SerHis: 0.902 ± 0.029
2.471SerIle: 2.471 ± 0.046
1.31SerLys: 1.31 ± 0.039
4.819SerLeu: 4.819 ± 0.07
1.055SerMet: 1.055 ± 0.028
1.291SerAsn: 1.291 ± 0.036
2.823SerPro: 2.823 ± 0.059
1.391SerGln: 1.391 ± 0.038
3.621SerArg: 3.621 ± 0.057
2.506SerSer: 2.506 ± 0.05
2.668SerThr: 2.668 ± 0.053
3.679SerVal: 3.679 ± 0.054
0.818SerTrp: 0.818 ± 0.03
1.335SerTyr: 1.335 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
6.898ThrAla: 6.898 ± 0.1
0.347ThrCys: 0.347 ± 0.02
2.906ThrAsp: 2.906 ± 0.051
2.43ThrGlu: 2.43 ± 0.046
1.686ThrPhe: 1.686 ± 0.041
5.436ThrGly: 5.436 ± 0.082
0.914ThrHis: 0.914 ± 0.027
2.85ThrIle: 2.85 ± 0.049
1.221ThrLys: 1.221 ± 0.033
6.266ThrLeu: 6.266 ± 0.076
1.095ThrMet: 1.095 ± 0.031
1.26ThrAsn: 1.26 ± 0.04
3.727ThrPro: 3.727 ± 0.065
1.569ThrGln: 1.569 ± 0.036
3.858ThrArg: 3.858 ± 0.057
2.648ThrSer: 2.648 ± 0.048
2.778ThrThr: 2.778 ± 0.058
4.386ThrVal: 4.386 ± 0.063
0.607ThrTrp: 0.607 ± 0.023
1.205ThrTyr: 1.205 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
10.673ValAla: 10.673 ± 0.114
0.523ValCys: 0.523 ± 0.02
4.42ValAsp: 4.42 ± 0.062
4.779ValGlu: 4.779 ± 0.067
2.165ValPhe: 2.165 ± 0.047
5.749ValGly: 5.749 ± 0.07
1.352ValHis: 1.352 ± 0.036
3.171ValIle: 3.171 ± 0.05
1.795ValLys: 1.795 ± 0.044
7.262ValLeu: 7.262 ± 0.096
1.427ValMet: 1.427 ± 0.036
1.881ValAsn: 1.881 ± 0.044
4.248ValPro: 4.248 ± 0.064
2.254ValGln: 2.254 ± 0.048
5.79ValArg: 5.79 ± 0.077
3.887ValSer: 3.887 ± 0.057
4.665ValThr: 4.665 ± 0.064
5.681ValVal: 5.681 ± 0.09
0.823ValTrp: 0.823 ± 0.025
1.407ValTyr: 1.407 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.481TrpAla: 1.481 ± 0.037
0.12TrpCys: 0.12 ± 0.011
0.747TrpAsp: 0.747 ± 0.029
0.629TrpGlu: 0.629 ± 0.024
0.594TrpPhe: 0.594 ± 0.025
0.982TrpGly: 0.982 ± 0.031
0.363TrpHis: 0.363 ± 0.02
0.677TrpIle: 0.677 ± 0.024
0.456TrpLys: 0.456 ± 0.02
1.709TrpLeu: 1.709 ± 0.043
0.348TrpMet: 0.348 ± 0.016
0.441TrpAsn: 0.441 ± 0.018
0.701TrpPro: 0.701 ± 0.027
0.651TrpGln: 0.651 ± 0.024
1.435TrpArg: 1.435 ± 0.037
0.892TrpSer: 0.892 ± 0.033
0.866TrpThr: 0.866 ± 0.028
0.908TrpVal: 0.908 ± 0.029
0.285TrpTrp: 0.285 ± 0.018
0.323TrpTyr: 0.323 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.82TyrAla: 2.82 ± 0.046
0.201TyrCys: 0.201 ± 0.013
1.542TyrAsp: 1.542 ± 0.037
1.107TyrGlu: 1.107 ± 0.033
0.809TyrPhe: 0.809 ± 0.024
2.106TyrGly: 2.106 ± 0.041
0.454TyrHis: 0.454 ± 0.02
0.667TyrIle: 0.667 ± 0.025
0.524TyrLys: 0.524 ± 0.024
2.16TyrLeu: 2.16 ± 0.045
0.361TyrMet: 0.361 ± 0.016
0.623TyrAsn: 0.623 ± 0.023
1.102TyrPro: 1.102 ± 0.036
0.702TyrGln: 0.702 ± 0.03
2.074TyrArg: 2.074 ± 0.044
1.114TyrSer: 1.114 ± 0.033
1.023TyrThr: 1.023 ± 0.035
1.605TyrVal: 1.605 ± 0.035
0.341TyrTrp: 0.341 ± 0.018
0.651TyrTyr: 0.651 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3641 proteins (1165691 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski