Halomonas axialensis
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5488 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A455VCS9|A0A455VCS9_9GAMM Uncharacterized protein OS=Halomonas axialensis OX=115555 GN=HAALTHF_31320n PE=4 SV=2
MM1 pKa = 6.98 TRR3 pKa = 11.84 SNRR6 pKa = 11.84 SALKK10 pKa = 10.32 SLSLAPLTAALLASSLTITPTQAQEE35 pKa = 3.78 RR36 pKa = 11.84 SDD38 pKa = 5.84 FSVCWSIYY46 pKa = 9.54 AGWMPWAYY54 pKa = 10.59 ADD56 pKa = 4.27 VEE58 pKa = 4.6 GVVNKK63 pKa = 9.46 WADD66 pKa = 3.48 EE67 pKa = 4.03 YY68 pKa = 11.37 GISIDD73 pKa = 3.57 VVQVNDD79 pKa = 3.58 YY80 pKa = 10.95 VEE82 pKa = 5.1 SINQFTSGNVDD93 pKa = 3.21 GCAMTNMDD101 pKa = 5.17 ALTIPAASGVDD112 pKa = 3.58 STALIINDD120 pKa = 3.71 YY121 pKa = 11.52 SNGNDD126 pKa = 4.06 GIVLKK131 pKa = 11.0 GSDD134 pKa = 4.6 DD135 pKa = 3.87 LADD138 pKa = 3.16 IAGRR142 pKa = 11.84 QVNLVQFSVSHH153 pKa = 5.55 YY154 pKa = 8.95 FLARR158 pKa = 11.84 ALEE161 pKa = 4.48 SVGLTEE167 pKa = 5.89 RR168 pKa = 11.84 DD169 pKa = 3.11 IEE171 pKa = 4.52 TVNTSDD177 pKa = 3.85 ADD179 pKa = 3.65 IVGLFASDD187 pKa = 3.16 TTEE190 pKa = 6.07 AVAAWNPQLGAIEE203 pKa = 5.34 DD204 pKa = 3.95 MADD207 pKa = 3.41 SNVVYY212 pKa = 9.71 TSAEE216 pKa = 3.85 IPGEE220 pKa = 4.05 ILDD223 pKa = 3.75 MMVVNTQTLADD234 pKa = 3.97 NPALGHH240 pKa = 6.36 ALVGAWYY247 pKa = 9.64 EE248 pKa = 3.99 VMGLIEE254 pKa = 5.73 ADD256 pKa = 3.96 DD257 pKa = 4.17 EE258 pKa = 4.42 NALGIMADD266 pKa = 3.6 AAGTDD271 pKa = 2.98 IDD273 pKa = 5.15 GYY275 pKa = 9.8 RR276 pKa = 11.84 QQLAATYY283 pKa = 9.81 LYY285 pKa = 9.65 TDD287 pKa = 3.81 PAEE290 pKa = 5.18 AIALMEE296 pKa = 4.44 SPEE299 pKa = 4.85 LLDD302 pKa = 3.07 TMQRR306 pKa = 11.84 VAEE309 pKa = 4.39 FSFQHH314 pKa = 6.2 GLLGDD319 pKa = 3.89 MAPNAEE325 pKa = 4.31 VVGIEE330 pKa = 4.22 TPSGVFGDD338 pKa = 3.95 EE339 pKa = 4.5 SNVQLRR345 pKa = 11.84 FDD347 pKa = 3.75 PSFTQAYY354 pKa = 10.42 LDD356 pKa = 3.75 AQQ358 pKa = 3.73
Molecular weight: 38.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.63
IPC_protein 3.656
Toseland 3.427
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.478
Grimsley 3.338
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.592
Sillero 3.77
Patrickios 1.176
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A510J396|A0A510J396_9GAMM Ni_hydr_CYTB domain-containing protein OS=Halomonas axialensis OX=115555 GN=HAALTHF_16550n PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 NGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5488
0
5488
1002324
39
1404
182.6
20.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.866 ± 0.045
1.104 ± 0.013
5.257 ± 0.035
6.097 ± 0.038
3.438 ± 0.027
7.696 ± 0.032
2.468 ± 0.019
4.847 ± 0.029
3.198 ± 0.029
11.014 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.811 ± 0.022
2.887 ± 0.024
4.969 ± 0.028
4.384 ± 0.027
6.725 ± 0.036
5.856 ± 0.027
5.286 ± 0.026
7.082 ± 0.034
1.555 ± 0.019
2.461 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here