Apple rubbery wood virus 2
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1B5C1|A0A2S1B5C1_9VIRU Isoform of A0A2S1B5H3 Nucleocapsid protein OS=Apple rubbery wood virus 2 OX=2164103 GN=CP PE=4 SV=1
MM1 pKa = 7.36 SASIMQRR8 pKa = 11.84 IDD10 pKa = 3.45 LEE12 pKa = 4.48 NVCDD16 pKa = 3.8 LTYY19 pKa = 11.01 EE20 pKa = 4.62 GVFSSDD26 pKa = 2.83 MPINIEE32 pKa = 4.04 YY33 pKa = 9.76 VHH35 pKa = 6.75 EE36 pKa = 4.56 GNYY39 pKa = 8.84 IRR41 pKa = 11.84 LMNKK45 pKa = 8.7 NLEE48 pKa = 4.08 YY49 pKa = 10.75 EE50 pKa = 4.48 LIKK53 pKa = 10.05 IDD55 pKa = 3.81 YY56 pKa = 8.78 NNPNFIHH63 pKa = 7.28 DD64 pKa = 4.15 CVSYY68 pKa = 10.73 AISGQFKK75 pKa = 10.27 TDD77 pKa = 3.18 MTSYY81 pKa = 11.34 GLTPDD86 pKa = 4.2 FVSEE90 pKa = 4.36 DD91 pKa = 3.62 FLLEE95 pKa = 3.79 ISTSSVSDD103 pKa = 4.11 FHH105 pKa = 8.64 ALEE108 pKa = 4.04 IQMQSKK114 pKa = 9.46 LKK116 pKa = 10.38 KK117 pKa = 8.97 YY118 pKa = 10.32 AHH120 pKa = 6.87 IANGRR125 pKa = 11.84 KK126 pKa = 9.85 VIVIVISYY134 pKa = 10.58 DD135 pKa = 3.62 LVVSNYY141 pKa = 9.44 EE142 pKa = 3.74 IKK144 pKa = 10.92 KK145 pKa = 8.5 NIQMAMTSNFCILKK159 pKa = 10.02 RR160 pKa = 11.84 LQSEE164 pKa = 4.32 LLMEE168 pKa = 5.08 GKK170 pKa = 8.77 TYY172 pKa = 7.58 WTTSSEE178 pKa = 4.47 TKK180 pKa = 10.63 FSRR183 pKa = 11.84 KK184 pKa = 9.62 LSTILQNYY192 pKa = 7.19 VPKK195 pKa = 10.66 NHH197 pKa = 6.68 YY198 pKa = 9.62 DD199 pKa = 3.45 GKK201 pKa = 10.7 KK202 pKa = 9.63 QAFDD206 pKa = 5.43 LIDD209 pKa = 3.73 QVWEE213 pKa = 3.91 NFDD216 pKa = 3.7 YY217 pKa = 10.97 DD218 pKa = 3.75 SSLGQVLTEE227 pKa = 4.14 VVKK230 pKa = 10.83 HH231 pKa = 5.72 VEE233 pKa = 4.51 DD234 pKa = 6.32 DD235 pKa = 2.94 ITTAKK240 pKa = 10.47 FIEE243 pKa = 4.94 KK244 pKa = 9.19 PPSPISFPTEE254 pKa = 3.12 GRR256 pKa = 11.84 TDD258 pKa = 3.83 LKK260 pKa = 10.64 RR261 pKa = 11.84 ITVIPMICPEE271 pKa = 4.1 KK272 pKa = 10.37 RR273 pKa = 11.84 VSLEE277 pKa = 3.93 LLEE280 pKa = 4.53 TTCVNSLIAEE290 pKa = 4.27 SKK292 pKa = 10.35 IKK294 pKa = 10.23 IIEE297 pKa = 4.19 EE298 pKa = 3.95 GKK300 pKa = 8.38 ITQKK304 pKa = 8.65 TQIDD308 pKa = 4.17 FATPMTHH315 pKa = 6.58 FEE317 pKa = 4.19 IMSGYY322 pKa = 10.52 YY323 pKa = 9.2 EE324 pKa = 5.44 DD325 pKa = 4.15 KK326 pKa = 11.22 RR327 pKa = 11.84 EE328 pKa = 4.05 DD329 pKa = 3.21 KK330 pKa = 10.82 KK331 pKa = 11.12 EE332 pKa = 3.85 RR333 pKa = 11.84 KK334 pKa = 9.47 GYY336 pKa = 7.49 QFKK339 pKa = 11.01 PEE341 pKa = 4.09 LTPKK345 pKa = 10.55 EE346 pKa = 4.07 MLEE349 pKa = 4.22 LNLDD353 pKa = 3.36 GVMAKK358 pKa = 9.34 EE359 pKa = 4.47 FKK361 pKa = 10.58 DD362 pKa = 3.09 KK363 pKa = 11.36 TEE365 pKa = 4.5 IIEE368 pKa = 3.95 KK369 pKa = 9.88 RR370 pKa = 11.84 QLSKK374 pKa = 10.87 IPFSFNVDD382 pKa = 3.29 TQDD385 pKa = 3.09 IEE387 pKa = 4.53 SFRR390 pKa = 11.84 LEE392 pKa = 3.96 FAKK395 pKa = 11.04 LLDD398 pKa = 3.91 EE399 pKa = 4.36 KK400 pKa = 11.31 VEE402 pKa = 4.24 NVVEE406 pKa = 4.12 DD407 pKa = 3.45 QAYY410 pKa = 9.79 GLMRR414 pKa = 11.84 TAYY417 pKa = 10.25 SCMIDD422 pKa = 3.13 AHH424 pKa = 8.28 VNMIDD429 pKa = 3.37 QGEE432 pKa = 4.21 NVIKK436 pKa = 10.19 EE437 pKa = 4.29 HH438 pKa = 7.07 NEE440 pKa = 3.62 EE441 pKa = 4.16 NSTKK445 pKa = 10.39 FINWLNFVSDD455 pKa = 4.94 LMFEE459 pKa = 4.45 INLSLRR465 pKa = 11.84 EE466 pKa = 3.94 NTKK469 pKa = 10.67 NGFFIVKK476 pKa = 9.15 SLKK479 pKa = 10.19 RR480 pKa = 11.84 FQADD484 pKa = 3.57 VYY486 pKa = 11.14 IKK488 pKa = 7.43 TTNSNSHH495 pKa = 5.8 IFFFVVCKK503 pKa = 10.62 VNYY506 pKa = 9.56 ILSDD510 pKa = 3.3 TLGPKK515 pKa = 10.07 FVTSNGILVTEE526 pKa = 4.89 WGSLMKK532 pKa = 10.11 RR533 pKa = 11.84 DD534 pKa = 3.97 LEE536 pKa = 4.26 NFLSCKK542 pKa = 7.52 EE543 pKa = 4.36 TIRR546 pKa = 11.84 STFCCYY552 pKa = 10.84 DD553 pKa = 3.68 EE554 pKa = 6.44 IFDD557 pKa = 4.15 KK558 pKa = 11.09 CHH560 pKa = 6.84 NSALTNAKK568 pKa = 8.87 WLSLMIFLSNKK579 pKa = 9.32 DD580 pKa = 3.57 RR581 pKa = 11.84 NEE583 pKa = 4.25 EE584 pKa = 3.92 IVTSLRR590 pKa = 11.84 YY591 pKa = 8.27 TYY593 pKa = 9.51 MKK595 pKa = 10.09 SLSITEE601 pKa = 4.75 HH602 pKa = 6.39 SSTMNKK608 pKa = 10.13 KK609 pKa = 10.41 LGLLMRR615 pKa = 11.84 DD616 pKa = 3.16 RR617 pKa = 11.84 LQVYY621 pKa = 7.58 LTNKK625 pKa = 9.71 VYY627 pKa = 11.03 LLNNFLTDD635 pKa = 3.29 NKK637 pKa = 9.63 PQVSFDD643 pKa = 3.25 QGTLEE648 pKa = 3.95 VHH650 pKa = 6.0 GMKK653 pKa = 10.53 DD654 pKa = 3.49 LFGSDD659 pKa = 2.62 ITFKK663 pKa = 10.9 EE664 pKa = 4.9 GINISYY670 pKa = 10.32 AGYY673 pKa = 10.59 IMTKK677 pKa = 10.01 VKK679 pKa = 10.36 QPQSNQSSKK688 pKa = 10.43 MIEE691 pKa = 4.26 KK692 pKa = 10.32 ILDD695 pKa = 3.58 YY696 pKa = 10.51 EE697 pKa = 4.44 IKK699 pKa = 10.23 FLNVKK704 pKa = 10.46 AEE706 pKa = 4.25 EE707 pKa = 4.16 ISTNEE712 pKa = 3.28 LNIRR716 pKa = 11.84 KK717 pKa = 8.63 DD718 pKa = 2.94 HH719 pKa = 6.33 CFDD722 pKa = 3.35 YY723 pKa = 10.79 KK724 pKa = 10.21 YY725 pKa = 11.29 VRR727 pKa = 11.84 TFSEE731 pKa = 4.1 LGKK734 pKa = 10.44 EE735 pKa = 4.04 LLYY738 pKa = 11.34 DD739 pKa = 3.46 MFGNQLDD746 pKa = 4.0 SIIRR750 pKa = 11.84 EE751 pKa = 4.13 RR752 pKa = 11.84 ITSNLMSIDD761 pKa = 3.79 FEE763 pKa = 5.24 SIATLKK769 pKa = 11.01 ASAKK773 pKa = 8.97 PDD775 pKa = 3.35 LRR777 pKa = 11.84 GKK779 pKa = 10.53 YY780 pKa = 9.67 SEE782 pKa = 4.69 EE783 pKa = 3.67 NLEE786 pKa = 4.18 EE787 pKa = 4.3 LKK789 pKa = 10.73 SARR792 pKa = 11.84 PRR794 pKa = 11.84 VMSNIVKK801 pKa = 10.45 LINTYY806 pKa = 10.36 KK807 pKa = 10.23 PEE809 pKa = 4.02 HH810 pKa = 6.61 FIDD813 pKa = 4.22 LVEE816 pKa = 4.12 PCISYY821 pKa = 10.12 IVNNNCFNVEE831 pKa = 4.66 LFPKK835 pKa = 9.85 DD836 pKa = 3.12 QHH838 pKa = 6.23 NGLRR842 pKa = 11.84 EE843 pKa = 3.9 IYY845 pKa = 10.54 VVDD848 pKa = 2.98 IHH850 pKa = 8.08 ARR852 pKa = 11.84 IVQLFVEE859 pKa = 5.23 TISRR863 pKa = 11.84 SLCSFFQSEE872 pKa = 4.59 TLNHH876 pKa = 6.83 PKK878 pKa = 10.51 NSTALIPKK886 pKa = 6.53 TLKK889 pKa = 10.76 YY890 pKa = 10.63 SLEE893 pKa = 4.28 NNGSPFCLMKK903 pKa = 10.93 SGDD906 pKa = 3.55 ASKK909 pKa = 10.2 WSQSQIVTKK918 pKa = 9.62 FYY920 pKa = 10.73 IVLKK924 pKa = 10.67 AFLPQEE930 pKa = 3.65 YY931 pKa = 10.21 HH932 pKa = 7.21 KK933 pKa = 10.51 IVKK936 pKa = 9.87 DD937 pKa = 3.96 ALSLWFDD944 pKa = 3.64 KK945 pKa = 11.05 KK946 pKa = 10.79 IFLPPSLIEE955 pKa = 4.56 SFMGTNFHH963 pKa = 6.53 TSNVVLNDD971 pKa = 3.24 LRR973 pKa = 11.84 EE974 pKa = 4.3 GNLCAIKK981 pKa = 10.43 KK982 pKa = 9.81 KK983 pKa = 10.51 EE984 pKa = 4.04 DD985 pKa = 3.31 MFMTIQSGFMQGILHH1000 pKa = 6.59 HH1001 pKa = 6.7 SSSLFHH1007 pKa = 6.72 TIVQEE1012 pKa = 4.17 GFRR1015 pKa = 11.84 QIQFKK1020 pKa = 9.08 VLRR1023 pKa = 11.84 DD1024 pKa = 3.66 AFNDD1028 pKa = 4.3 TIYY1031 pKa = 10.67 KK1032 pKa = 10.16 QYY1034 pKa = 10.5 FEE1036 pKa = 4.68 KK1037 pKa = 11.29 VNICVLQGSDD1047 pKa = 3.94 DD1048 pKa = 3.83 SSMAIMGDD1056 pKa = 2.86 SKK1058 pKa = 11.11 LFHH1061 pKa = 6.17 KK1062 pKa = 10.37 HH1063 pKa = 5.88 KK1064 pKa = 10.66 EE1065 pKa = 4.02 LFLSLMHH1072 pKa = 6.89 LKK1074 pKa = 10.76 DD1075 pKa = 3.34 SLSYY1079 pKa = 10.7 KK1080 pKa = 10.48 FGIIPSAEE1088 pKa = 3.94 KK1089 pKa = 9.54 TATCVPFIIEE1099 pKa = 4.74 YY1100 pKa = 9.63 NSQWLALGKK1109 pKa = 10.31 SIQPTIKK1116 pKa = 9.81 FAMVACQLPSAQSIIQRR1133 pKa = 11.84 QEE1135 pKa = 3.29 FYY1137 pKa = 11.6 YY1138 pKa = 11.12 NLLKK1142 pKa = 10.89 DD1143 pKa = 4.22 CLEE1146 pKa = 4.63 KK1147 pKa = 11.21 GCTTHH1152 pKa = 6.27 TCFKK1156 pKa = 10.54 IKK1158 pKa = 10.54 LNQLCAYY1165 pKa = 9.84 YY1166 pKa = 10.99 NLLGCNNTSSFQVTSKK1182 pKa = 10.89 LLKK1185 pKa = 9.74 EE1186 pKa = 4.57 CLNPSLGFFMLEE1198 pKa = 4.25 PIVATAVFGFDD1209 pKa = 3.45 FNFYY1213 pKa = 10.64 CHH1215 pKa = 5.91 TQGALVKK1222 pKa = 8.97 YY1223 pKa = 10.0 LPNSYY1228 pKa = 8.22 HH1229 pKa = 6.42 TPMEE1233 pKa = 4.37 TNLPSGNTLRR1243 pKa = 11.84 LNTHH1247 pKa = 6.13 ISKK1250 pKa = 10.75 SGWKK1254 pKa = 8.93 KK1255 pKa = 8.5 WKK1257 pKa = 10.01 QLVEE1261 pKa = 4.26 EE1262 pKa = 4.25 VGEE1265 pKa = 4.07 LSSEE1269 pKa = 4.02 EE1270 pKa = 3.88 EE1271 pKa = 4.07 QYY1273 pKa = 11.56 LEE1275 pKa = 4.34 NNPMLYY1281 pKa = 10.1 YY1282 pKa = 10.27 EE1283 pKa = 4.46 KK1284 pKa = 10.69 SRR1286 pKa = 11.84 NDD1288 pKa = 3.25 VYY1290 pKa = 11.68 SSVIKK1295 pKa = 10.57 IKK1297 pKa = 10.2 QKK1299 pKa = 10.97 CQDD1302 pKa = 3.33 FDD1304 pKa = 4.07 VKK1306 pKa = 10.87 QSLSSRR1312 pKa = 11.84 YY1313 pKa = 10.16 GITEE1317 pKa = 3.53 NMASFFCTFIKK1328 pKa = 10.09 VCKK1331 pKa = 9.63 IGKK1334 pKa = 9.37 EE1335 pKa = 3.85 KK1336 pKa = 10.89 DD1337 pKa = 3.66 SLVGWMLKK1345 pKa = 8.96 CTEE1348 pKa = 4.55 NGISDD1353 pKa = 4.71 LSQMFPKK1360 pKa = 10.44 FKK1362 pKa = 10.25 LYY1364 pKa = 11.08 NEE1366 pKa = 4.81 IIHH1369 pKa = 6.23 YY1370 pKa = 10.09 ISVISNQSYY1379 pKa = 10.23 LVDD1382 pKa = 2.85 VDD1384 pKa = 3.77 YY1385 pKa = 10.93 IQRR1388 pKa = 11.84 GRR1390 pKa = 11.84 SQITVLHH1397 pKa = 6.35 NKK1399 pKa = 8.48 TSEE1402 pKa = 4.12 LEE1404 pKa = 4.03 LFYY1407 pKa = 10.77 EE1408 pKa = 4.53 TVKK1411 pKa = 11.03 NVWFDD1416 pKa = 3.63 VEE1418 pKa = 4.33 CDD1420 pKa = 3.89 EE1421 pKa = 4.44 ITKK1424 pKa = 10.29 DD1425 pKa = 2.81 AYY1427 pKa = 10.32 RR1428 pKa = 11.84 RR1429 pKa = 11.84 LFRR1432 pKa = 11.84 EE1433 pKa = 3.9 YY1434 pKa = 10.06 MEE1436 pKa = 4.4 EE1437 pKa = 3.96 FDD1439 pKa = 3.49 WLKK1442 pKa = 8.87 WTEE1445 pKa = 4.11 KK1446 pKa = 7.95 EE1447 pKa = 4.08 TKK1449 pKa = 10.53 AFLNCSSRR1457 pKa = 11.84 QLANYY1462 pKa = 8.41 VRR1464 pKa = 11.84 NVVGKK1469 pKa = 9.99 NRR1471 pKa = 11.84 TLTFFDD1477 pKa = 4.04 TVAKK1481 pKa = 10.09 GSNYY1485 pKa = 9.73 PYY1487 pKa = 10.73 LLTRR1491 pKa = 11.84 IFQKK1495 pKa = 10.57 DD1496 pKa = 3.13 KK1497 pKa = 10.61 KK1498 pKa = 9.27 IQVVTGKK1505 pKa = 10.25 DD1506 pKa = 3.23 VSINKK1511 pKa = 9.45 NDD1513 pKa = 3.92 IIFSNMKK1520 pKa = 9.79 ILLNLPYY1527 pKa = 10.17 KK1528 pKa = 10.39 EE1529 pKa = 4.14 SYY1531 pKa = 10.47 KK1532 pKa = 10.6 MNTLKK1537 pKa = 10.71 EE1538 pKa = 4.7 LIPKK1542 pKa = 9.94 LDD1544 pKa = 3.72 LSEE1547 pKa = 4.27 LTSKK1551 pKa = 9.76 EE1552 pKa = 3.68 AKK1554 pKa = 9.62 KK1555 pKa = 11.06 YY1556 pKa = 10.6 KK1557 pKa = 9.84 ILKK1560 pKa = 9.93 LVLSDD1565 pKa = 3.99 KK1566 pKa = 11.37 KK1567 pKa = 10.97 EE1568 pKa = 4.0 DD1569 pKa = 3.68 VKK1571 pKa = 11.64 NEE1573 pKa = 3.61 LFKK1576 pKa = 11.57 YY1577 pKa = 10.39 SFEE1580 pKa = 4.22 NHH1582 pKa = 5.35 LTWYY1586 pKa = 10.31 SQAQHH1591 pKa = 6.07 FNKK1594 pKa = 9.95 EE1595 pKa = 3.93 NKK1597 pKa = 7.85 SWEE1600 pKa = 4.66 GIGCLTTIISTLTIKK1615 pKa = 9.97 TIMMDD1620 pKa = 3.46 SLIQEE1625 pKa = 4.56 IIVNDD1630 pKa = 4.07 LSSFKK1635 pKa = 10.85 LHH1637 pKa = 6.92 LDD1639 pKa = 3.23 VYY1641 pKa = 9.08 MKK1643 pKa = 10.26 IIKK1646 pKa = 7.7 EE1647 pKa = 4.16 HH1648 pKa = 6.32 KK1649 pKa = 10.39 LEE1651 pKa = 5.16 LGDD1654 pKa = 5.64 NVDD1657 pKa = 5.85 DD1658 pKa = 4.86 DD1659 pKa = 5.35 LKK1661 pKa = 10.8 LSKK1664 pKa = 11.04 YY1665 pKa = 10.44 GISKK1669 pKa = 10.04 SFKK1672 pKa = 8.86 GSKK1675 pKa = 9.67 IKK1677 pKa = 10.38 ISPEE1681 pKa = 3.73 LKK1683 pKa = 10.47 SNVAFLEE1690 pKa = 4.01 NSTLALTNGNMKK1702 pKa = 10.34 LVVPMIDD1709 pKa = 2.85 IDD1711 pKa = 4.52 SNFVEE1716 pKa = 5.34 KK1717 pKa = 10.63 RR1718 pKa = 11.84 NCVQTLYY1725 pKa = 10.85 LIEE1728 pKa = 4.46 SKK1730 pKa = 10.66 RR1731 pKa = 11.84 LARR1734 pKa = 11.84 TFQEE1738 pKa = 5.14 RR1739 pKa = 11.84 RR1740 pKa = 11.84 DD1741 pKa = 3.78 ADD1743 pKa = 3.6 HH1744 pKa = 6.85 NGYY1747 pKa = 9.92 IIEE1750 pKa = 4.88 NIWIDD1755 pKa = 3.27 SRR1757 pKa = 11.84 SYY1759 pKa = 10.24 NKK1761 pKa = 9.19 VRR1763 pKa = 11.84 HH1764 pKa = 5.44 GEE1766 pKa = 4.08 IIKK1769 pKa = 10.61 SRR1771 pKa = 11.84 MIMGGKK1777 pKa = 9.17 DD1778 pKa = 3.38 VKK1780 pKa = 10.87 LSTIMNFEE1788 pKa = 3.88 VWKK1791 pKa = 10.71 KK1792 pKa = 8.78 ITLPTNEE1799 pKa = 5.11 KK1800 pKa = 10.52 LFDD1803 pKa = 3.41 WGEE1806 pKa = 3.92 LGEE1809 pKa = 4.95 KK1810 pKa = 9.46 IVKK1813 pKa = 9.78 QEE1815 pKa = 3.96 SLDD1818 pKa = 3.64 FQSYY1822 pKa = 9.78 VLTKK1826 pKa = 10.68 LEE1828 pKa = 4.32 SFPKK1832 pKa = 10.31 RR1833 pKa = 11.84 MPTNEE1838 pKa = 4.24 MFHH1841 pKa = 7.63 QSMEE1845 pKa = 4.03 QNYY1848 pKa = 9.08 FDD1850 pKa = 4.22 CKK1852 pKa = 10.5 EE1853 pKa = 3.85 YY1854 pKa = 11.2 LNFISRR1860 pKa = 11.84 VEE1862 pKa = 4.04 FFQQVEE1868 pKa = 4.19 DD1869 pKa = 3.98 PDD1871 pKa = 3.91 YY1872 pKa = 11.85 DD1873 pKa = 3.9 EE1874 pKa = 5.84 LVKK1877 pKa = 10.32 KK1878 pKa = 10.65 DD1879 pKa = 3.56 KK1880 pKa = 11.26 EE1881 pKa = 4.23 EE1882 pKa = 4.34 EE1883 pKa = 3.9 FDD1885 pKa = 3.27 KK1886 pKa = 11.34 TLIDD1890 pKa = 3.78 RR1891 pKa = 11.84 FNEE1894 pKa = 4.32 LVFNTQALKK1903 pKa = 10.56 RR1904 pKa = 11.84 AIQKK1908 pKa = 7.91 TVEE1911 pKa = 4.08 FDD1913 pKa = 3.26 SCLINLDD1920 pKa = 3.61 NVLMKK1925 pKa = 10.79 AQTDD1929 pKa = 3.99 FSYY1932 pKa = 10.86 CRR1934 pKa = 11.84 HH1935 pKa = 5.72 LVYY1938 pKa = 10.39 GIKK1941 pKa = 9.61 DD1942 pKa = 3.64 TEE1944 pKa = 4.39 TTFPNTLFYY1953 pKa = 10.96 KK1954 pKa = 10.45 FNHH1957 pKa = 5.38 EE1958 pKa = 3.94 LRR1960 pKa = 11.84 RR1961 pKa = 11.84 RR1962 pKa = 11.84 IPDD1965 pKa = 3.25 NDD1967 pKa = 3.34 EE1968 pKa = 4.04 LLEE1971 pKa = 4.15 VMSKK1975 pKa = 10.96 GDD1977 pKa = 3.64 SGNLLTIYY1985 pKa = 9.83 QQIALNLLFATPTDD1999 pKa = 4.12 FTSLTTVKK2007 pKa = 10.25 SLRR2010 pKa = 11.84 EE2011 pKa = 4.12 FILLGYY2017 pKa = 9.12 KK2018 pKa = 10.12 PKK2020 pKa = 10.32 EE2021 pKa = 4.19 SRR2023 pKa = 11.84 SSRR2026 pKa = 11.84 SSTRR2030 pKa = 11.84 LGIYY2034 pKa = 10.01 LPNNDD2039 pKa = 5.26 LEE2041 pKa = 4.46 QKK2043 pKa = 9.37 MLSKK2047 pKa = 10.39 FQIHH2051 pKa = 6.76 PKK2053 pKa = 10.08 SEE2055 pKa = 3.93 QLFDD2059 pKa = 4.93 LLNAEE2064 pKa = 4.21 QKK2066 pKa = 10.87 LLEE2069 pKa = 4.09 EE2070 pKa = 5.54 AIDD2073 pKa = 3.92 EE2074 pKa = 4.17 VSQMIEE2080 pKa = 3.54 KK2081 pKa = 6.27 TTNRR2085 pKa = 11.84 RR2086 pKa = 11.84 VKK2088 pKa = 10.14 MPLLTQVVGYY2098 pKa = 10.66 EE2099 pKa = 4.13 EE2100 pKa = 4.52 DD2101 pKa = 3.96 LKK2103 pKa = 11.34 LLKK2106 pKa = 10.49 DD2107 pKa = 4.48 FRR2109 pKa = 11.84 AHH2111 pKa = 7.37 ALNLEE2116 pKa = 4.14 NEE2118 pKa = 4.53 KK2119 pKa = 10.74 EE2120 pKa = 4.24 SEE2122 pKa = 4.24 STKK2125 pKa = 11.15 DD2126 pKa = 3.96 SLEE2129 pKa = 4.22 CLDD2132 pKa = 4.36 NLEE2135 pKa = 4.86 EE2136 pKa = 4.29 NDD2138 pKa = 3.28 ISLSAEE2144 pKa = 4.11 EE2145 pKa = 4.65 EE2146 pKa = 4.28 DD2147 pKa = 5.79 DD2148 pKa = 5.45 DD2149 pKa = 4.56 LTHH2152 pKa = 7.1 SLTDD2156 pKa = 3.36 SDD2158 pKa = 3.91 EE2159 pKa = 4.44 SADD2162 pKa = 3.42 IGEE2165 pKa = 4.83 DD2166 pKa = 3.71 FNLLNHH2172 pKa = 6.71 LQNSYY2177 pKa = 10.87 KK2178 pKa = 9.95 EE2179 pKa = 4.19 KK2180 pKa = 10.74 KK2181 pKa = 8.85 VLKK2184 pKa = 9.88 RR2185 pKa = 11.84 NCFLYY2190 pKa = 11.07 LNVDD2194 pKa = 2.73 ITYY2197 pKa = 10.52 ILRR2200 pKa = 11.84 NPKK2203 pKa = 9.64 FDD2205 pKa = 4.41 IKK2207 pKa = 11.26 SQVQKK2212 pKa = 10.33 MNCTFHH2218 pKa = 7.87 LSDD2221 pKa = 3.33 MTILVNGAKK2230 pKa = 10.21 DD2231 pKa = 4.03 FEE2233 pKa = 5.11 VYY2235 pKa = 8.99 WPNDD2239 pKa = 3.22 DD2240 pKa = 3.76 SAEE2243 pKa = 3.88 FSIFYY2248 pKa = 10.46 HH2249 pKa = 5.39 STLPEE2254 pKa = 3.98 LPYY2257 pKa = 10.68 SQNILSKK2264 pKa = 9.43 EE2265 pKa = 3.84 IKK2267 pKa = 9.98 KK2268 pKa = 10.31 ISPLPGGSCLFNCFVYY2284 pKa = 10.87 LLSNHH2289 pKa = 5.67 GKK2291 pKa = 9.47 YY2292 pKa = 10.18 FKK2294 pKa = 10.48 QEE2296 pKa = 3.62 EE2297 pKa = 4.22 LRR2299 pKa = 11.84 QIVHH2303 pKa = 7.25 DD2304 pKa = 4.25 SDD2306 pKa = 3.75 HH2307 pKa = 6.96 LKK2309 pKa = 10.99 LLDD2312 pKa = 4.11 EE2313 pKa = 5.35 DD2314 pKa = 3.97 PHH2316 pKa = 8.16 KK2317 pKa = 10.85 KK2318 pKa = 10.48 AILSNDD2324 pKa = 2.67 EE2325 pKa = 3.86 WGQEE2329 pKa = 3.71 EE2330 pKa = 4.81 DD2331 pKa = 4.25 IEE2333 pKa = 4.92 TICHH2337 pKa = 5.82 EE2338 pKa = 4.59 LDD2340 pKa = 3.43 VSVLVVTDD2348 pKa = 3.73 YY2349 pKa = 11.68 AILQYY2354 pKa = 11.31 NEE2356 pKa = 3.98 TGQKK2360 pKa = 9.64 IGILINYY2367 pKa = 7.06 EE2368 pKa = 3.96 NVHH2371 pKa = 6.13 FDD2373 pKa = 3.33 VLEE2376 pKa = 4.05
Molecular weight: 276.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.573
IPC2_protein 5.588
IPC_protein 5.614
Toseland 5.893
ProMoST 5.881
Dawson 5.792
Bjellqvist 5.842
Wikipedia 5.766
Rodwell 5.766
Grimsley 5.995
Solomon 5.804
Lehninger 5.779
Nozaki 6.033
DTASelect 6.198
Thurlkill 6.16
EMBOSS 6.109
Sillero 6.122
Patrickios 4.126
IPC_peptide 5.817
IPC2_peptide 6.097
IPC2.peptide.svr19 6.164
Protein with the highest isoelectric point:
>tr|A0A2S1B6H7|A0A2S1B6H7_9VIRU Replicase OS=Apple rubbery wood virus 2 OX=2164103 GN=RdRp PE=4 SV=1
MM1 pKa = 7.86 AEE3 pKa = 4.3 GNFTLTGLTTALTLINHH20 pKa = 7.61 LLTVSATNFKK30 pKa = 10.5 AAAKK34 pKa = 10.36 ANARR38 pKa = 11.84 YY39 pKa = 8.24 FAYY42 pKa = 10.43 DD43 pKa = 3.53 GFNANEE49 pKa = 4.13 ILNAFITIFLTNTNSDD65 pKa = 3.72 DD66 pKa = 4.3 ALQDD70 pKa = 3.09 LCDD73 pKa = 3.63 IVNVGLTRR81 pKa = 11.84 GNIRR85 pKa = 11.84 EE86 pKa = 4.16 DD87 pKa = 3.53 QLKK90 pKa = 8.57 KK91 pKa = 8.08 TSKK94 pKa = 9.97 RR95 pKa = 11.84 GKK97 pKa = 9.71 EE98 pKa = 3.95 RR99 pKa = 11.84 IEE101 pKa = 4.06 EE102 pKa = 4.01 LSKK105 pKa = 10.9 KK106 pKa = 10.65 YY107 pKa = 10.4 NIKK110 pKa = 10.03 IKK112 pKa = 9.11 KK113 pKa = 7.08 TKK115 pKa = 10.39 NEE117 pKa = 4.05 SFTLSNSTLTFTRR130 pKa = 11.84 SISVFPYY137 pKa = 10.21 VASQLLATGSCILEE151 pKa = 3.94 QNNCKK156 pKa = 10.31 FGTDD160 pKa = 3.45 SLPAAFKK167 pKa = 10.7 HH168 pKa = 6.38 SGFASLIPNIADD180 pKa = 3.36 NNYY183 pKa = 10.05 CLVLFQCHH191 pKa = 4.36 VAYY194 pKa = 9.6 MISFGKK200 pKa = 10.19 KK201 pKa = 8.89 INPGNKK207 pKa = 9.48 DD208 pKa = 4.88 DD209 pKa = 5.52 DD210 pKa = 4.16 STWYY214 pKa = 10.15 KK215 pKa = 10.48 KK216 pKa = 10.02 QSQYY220 pKa = 11.62 SVAAWNNLSLYY231 pKa = 10.26 NNDD234 pKa = 3.21 YY235 pKa = 11.0 RR236 pKa = 11.84 KK237 pKa = 7.79 TAFKK241 pKa = 11.01 NLGLDD246 pKa = 3.27 SHH248 pKa = 6.52 GKK250 pKa = 10.09 YY251 pKa = 10.58 GEE253 pKa = 4.61 LYY255 pKa = 10.66 LKK257 pKa = 10.25 IVNNLRR263 pKa = 11.84 KK264 pKa = 9.65 SIKK267 pKa = 10.35 GEE269 pKa = 3.95 EE270 pKa = 4.14 VKK272 pKa = 10.6 PADD275 pKa = 3.88 LKK277 pKa = 11.3 PFIKK281 pKa = 10.03 EE282 pKa = 4.09 DD283 pKa = 4.78 KK284 pKa = 9.91 DD285 pKa = 4.1 LKK287 pKa = 9.51 WW288 pKa = 4.29
Molecular weight: 32.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.627
IPC2_protein 8.521
IPC_protein 8.39
Toseland 9.121
ProMoST 8.931
Dawson 9.428
Bjellqvist 9.209
Wikipedia 9.56
Rodwell 9.706
Grimsley 9.458
Solomon 9.472
Lehninger 9.443
Nozaki 9.355
DTASelect 9.136
Thurlkill 9.297
EMBOSS 9.575
Sillero 9.428
Patrickios 4.698
IPC_peptide 9.472
IPC2_peptide 7.805
IPC2.peptide.svr19 7.727
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
2
5
3720
286
2376
744.0
85.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.844 ± 1.228
1.72 ± 0.127
6.048 ± 0.212
7.554 ± 0.714
5.376 ± 0.274
4.032 ± 0.594
2.097 ± 0.22
7.043 ± 0.522
9.435 ± 0.347
10.161 ± 0.419
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.634 ± 0.389
6.667 ± 0.601
2.527 ± 0.211
3.253 ± 0.55
3.548 ± 0.509
8.091 ± 0.296
5.753 ± 0.389
5.349 ± 0.306
0.806 ± 0.087
4.059 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here