Amino acid dipepetide frequency for Staphylococcus massiliensis S46

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.613AlaAla: 3.613 ± 0.108	0.567AlaCys: 0.567 ± 0.033	2.826AlaAsp: 2.826 ± 0.083	3.436AlaGlu: 3.436 ± 0.084	3.196AlaPhe: 3.196 ± 0.077	3.94AlaGly: 3.94 ± 0.092	1.408AlaHis: 1.408 ± 0.046	5.925AlaIle: 5.925 ± 0.114	4.501AlaLys: 4.501 ± 0.098	6.962AlaLeu: 6.962 ± 0.142	1.844AlaMet: 1.844 ± 0.049	2.68AlaAsn: 2.68 ± 0.073	1.921AlaPro: 1.921 ± 0.072	2.259AlaGln: 2.259 ± 0.069	2.32AlaArg: 2.32 ± 0.071	4.066AlaSer: 4.066 ± 0.084	3.718AlaThr: 3.718 ± 0.106	3.944AlaVal: 3.944 ± 0.08	0.394AlaTrp: 0.394 ± 0.024	2.356AlaTyr: 2.356 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.366CysAla: 0.366 ± 0.025	0.087CysCys: 0.087 ± 0.011	0.417CysAsp: 0.417 ± 0.026	0.367CysGlu: 0.367 ± 0.026	0.27CysPhe: 0.27 ± 0.018	0.576CysGly: 0.576 ± 0.031	0.204CysHis: 0.204 ± 0.018	0.601CysIle: 0.601 ± 0.032	0.356CysLys: 0.356 ± 0.025	0.62CysLeu: 0.62 ± 0.034	0.182CysMet: 0.182 ± 0.017	0.262CysAsn: 0.262 ± 0.021	0.288CysPro: 0.288 ± 0.024	0.176CysGln: 0.176 ± 0.016	0.24CysArg: 0.24 ± 0.019	0.375CysSer: 0.375 ± 0.029	0.348CysThr: 0.348 ± 0.026	0.369CysVal: 0.369 ± 0.021	0.04CysTrp: 0.04 ± 0.008	0.242CysTyr: 0.242 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.985AspAla: 3.985 ± 0.114	0.357AspCys: 0.357 ± 0.023	3.912AspAsp: 3.912 ± 0.111	5.308AspGlu: 5.308 ± 0.107	2.704AspPhe: 2.704 ± 0.073	3.7AspGly: 3.7 ± 0.167	1.446AspHis: 1.446 ± 0.048	5.378AspIle: 5.378 ± 0.109	4.857AspLys: 4.857 ± 0.098	5.231AspLeu: 5.231 ± 0.094	1.69AspMet: 1.69 ± 0.054	2.801AspAsn: 2.801 ± 0.077	1.708AspPro: 1.708 ± 0.057	2.272AspGln: 2.272 ± 0.06	2.068AspArg: 2.068 ± 0.061	2.865AspSer: 2.865 ± 0.089	3.258AspThr: 3.258 ± 0.095	4.633AspVal: 4.633 ± 0.09	0.406AspTrp: 0.406 ± 0.026	2.667AspTyr: 2.667 ± 0.077	0.0AspXaa: 0.0 ± 0.0
Glu	5.383GluAla: 5.383 ± 0.12	0.285GluCys: 0.285 ± 0.023	4.621GluAsp: 4.621 ± 0.105	5.24GluGlu: 5.24 ± 0.101	2.234GluPhe: 2.234 ± 0.061	4.065GluGly: 4.065 ± 0.088	1.749GluHis: 1.749 ± 0.056	5.437GluIle: 5.437 ± 0.102	5.474GluLys: 5.474 ± 0.101	6.025GluLeu: 6.025 ± 0.1	2.138GluMet: 2.138 ± 0.063	3.444GluAsn: 3.444 ± 0.077	1.956GluPro: 1.956 ± 0.067	3.027GluGln: 3.027 ± 0.076	3.415GluArg: 3.415 ± 0.079	3.969GluSer: 3.969 ± 0.087	4.373GluThr: 4.373 ± 0.101	4.832GluVal: 4.832 ± 0.082	0.569GluTrp: 0.569 ± 0.032	2.088GluTyr: 2.088 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	2.338PheAla: 2.338 ± 0.076	0.279PheCys: 0.279 ± 0.02	2.91PheAsp: 2.91 ± 0.064	3.162PheGlu: 3.162 ± 0.078	2.062PhePhe: 2.062 ± 0.079	3.087PheGly: 3.087 ± 0.079	0.849PheHis: 0.849 ± 0.036	4.298PheIle: 4.298 ± 0.113	3.847PheLys: 3.847 ± 0.086	3.891PheLeu: 3.891 ± 0.109	1.233PheMet: 1.233 ± 0.043	2.701PheAsn: 2.701 ± 0.071	1.262PhePro: 1.262 ± 0.055	1.374PheGln: 1.374 ± 0.046	1.283PheArg: 1.283 ± 0.046	2.789PheSer: 2.789 ± 0.065	2.386PheThr: 2.386 ± 0.065	3.206PheVal: 3.206 ± 0.073	0.32PheTrp: 0.32 ± 0.021	1.66PheTyr: 1.66 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	4.267GlyAla: 4.267 ± 0.096	0.497GlyCys: 0.497 ± 0.027	3.439GlyAsp: 3.439 ± 0.093	3.898GlyGlu: 3.898 ± 0.081	3.127GlyPhe: 3.127 ± 0.076	4.247GlyGly: 4.247 ± 0.105	1.536GlyHis: 1.536 ± 0.046	5.419GlyIle: 5.419 ± 0.115	4.288GlyLys: 4.288 ± 0.082	6.138GlyLeu: 6.138 ± 0.113	1.825GlyMet: 1.825 ± 0.053	2.652GlyAsn: 2.652 ± 0.103	1.693GlyPro: 1.693 ± 0.057	2.22GlyGln: 2.22 ± 0.057	2.288GlyArg: 2.288 ± 0.063	3.583GlySer: 3.583 ± 0.095	3.756GlyThr: 3.756 ± 0.128	4.774GlyVal: 4.774 ± 0.099	0.476GlyTrp: 0.476 ± 0.027	2.685GlyTyr: 2.685 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.467HisAla: 1.467 ± 0.049	0.157HisCys: 0.157 ± 0.015	1.591HisAsp: 1.591 ± 0.052	1.621HisGlu: 1.621 ± 0.052	1.162HisPhe: 1.162 ± 0.044	1.405HisGly: 1.405 ± 0.054	0.879HisHis: 0.879 ± 0.044	2.047HisIle: 2.047 ± 0.058	1.521HisLys: 1.521 ± 0.047	2.342HisLeu: 2.342 ± 0.064	0.604HisMet: 0.604 ± 0.029	1.098HisAsn: 1.098 ± 0.035	1.098HisPro: 1.098 ± 0.047	1.123HisGln: 1.123 ± 0.046	0.924HisArg: 0.924 ± 0.039	1.18HisSer: 1.18 ± 0.04	1.246HisThr: 1.246 ± 0.045	1.688HisVal: 1.688 ± 0.054	0.157HisTrp: 0.157 ± 0.017	1.087HisTyr: 1.087 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	5.546IleAla: 5.546 ± 0.118	0.58IleCys: 0.58 ± 0.031	5.102IleAsp: 5.102 ± 0.083	6.136IleGlu: 6.136 ± 0.099	3.674IlePhe: 3.674 ± 0.098	5.813IleGly: 5.813 ± 0.122	1.843IleHis: 1.843 ± 0.053	6.826IleIle: 6.826 ± 0.151	5.95IleLys: 5.95 ± 0.102	7.438IleLeu: 7.438 ± 0.164	2.023IleMet: 2.023 ± 0.057	3.991IleAsn: 3.991 ± 0.084	3.059IlePro: 3.059 ± 0.074	2.954IleGln: 2.954 ± 0.065	2.661IleArg: 2.661 ± 0.081	5.103IleSer: 5.103 ± 0.1	4.732IleThr: 4.732 ± 0.101	5.829IleVal: 5.829 ± 0.12	0.55IleTrp: 0.55 ± 0.03	2.771IleTyr: 2.771 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	5.159LysAla: 5.159 ± 0.098	0.35LysCys: 0.35 ± 0.027	5.715LysAsp: 5.715 ± 0.116	6.326LysGlu: 6.326 ± 0.103	2.347LysPhe: 2.347 ± 0.062	4.523LysGly: 4.523 ± 0.092	2.001LysHis: 2.001 ± 0.055	4.566LysIle: 4.566 ± 0.094	5.778LysLys: 5.778 ± 0.15	6.029LysLeu: 6.029 ± 0.101	2.109LysMet: 2.109 ± 0.068	3.402LysAsn: 3.402 ± 0.09	2.582LysPro: 2.582 ± 0.067	3.268LysGln: 3.268 ± 0.069	3.782LysArg: 3.782 ± 0.082	4.508LysSer: 4.508 ± 0.091	3.743LysThr: 3.743 ± 0.076	5.111LysVal: 5.111 ± 0.104	0.664LysTrp: 0.664 ± 0.034	2.764LysTyr: 2.764 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	5.422LeuAla: 5.422 ± 0.111	0.66LeuCys: 0.66 ± 0.038	5.261LeuAsp: 5.261 ± 0.097	6.219LeuGlu: 6.219 ± 0.099	4.405LeuPhe: 4.405 ± 0.116	6.007LeuGly: 6.007 ± 0.101	1.844LeuHis: 1.844 ± 0.05	7.907LeuIle: 7.907 ± 0.168	7.509LeuLys: 7.509 ± 0.131	9.036LeuLeu: 9.036 ± 0.164	2.616LeuMet: 2.616 ± 0.056	5.557LeuAsn: 5.557 ± 0.107	3.522LeuPro: 3.522 ± 0.076	2.842LeuGln: 2.842 ± 0.078	2.996LeuArg: 2.996 ± 0.082	6.599LeuSer: 6.599 ± 0.113	5.51LeuThr: 5.51 ± 0.096	5.679LeuVal: 5.679 ± 0.106	0.611LeuTrp: 0.611 ± 0.031	2.962LeuTyr: 2.962 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	1.793MetAla: 1.793 ± 0.052	0.16MetCys: 0.16 ± 0.017	1.452MetAsp: 1.452 ± 0.044	1.449MetGlu: 1.449 ± 0.045	1.126MetPhe: 1.126 ± 0.046	1.444MetGly: 1.444 ± 0.052	0.649MetHis: 0.649 ± 0.028	2.361MetIle: 2.361 ± 0.059	2.511MetLys: 2.511 ± 0.07	2.416MetLeu: 2.416 ± 0.069	0.914MetMet: 0.914 ± 0.038	1.824MetAsn: 1.824 ± 0.051	0.983MetPro: 0.983 ± 0.039	0.82MetGln: 0.82 ± 0.039	1.108MetArg: 1.108 ± 0.044	1.946MetSer: 1.946 ± 0.056	1.76MetThr: 1.76 ± 0.047	1.612MetVal: 1.612 ± 0.052	0.169MetTrp: 0.169 ± 0.017	0.952MetTyr: 0.952 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.015AsnAla: 3.015 ± 0.076	0.267AsnCys: 0.267 ± 0.02	3.422AsnAsp: 3.422 ± 0.094	4.012AsnGlu: 4.012 ± 0.085	1.981AsnPhe: 1.981 ± 0.057	3.175AsnGly: 3.175 ± 0.092	1.456AsnHis: 1.456 ± 0.05	3.838AsnIle: 3.838 ± 0.087	3.76AsnLys: 3.76 ± 0.084	4.247AsnLeu: 4.247 ± 0.08	1.258AsnMet: 1.258 ± 0.045	2.58AsnAsn: 2.58 ± 0.093	2.063AsnPro: 2.063 ± 0.063	2.626AsnGln: 2.626 ± 0.083	2.029AsnArg: 2.029 ± 0.062	2.242AsnSer: 2.242 ± 0.084	2.441AsnThr: 2.441 ± 0.082	3.513AsnVal: 3.513 ± 0.084	0.429AsnTrp: 0.429 ± 0.025	2.138AsnTyr: 2.138 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	1.491ProAla: 1.491 ± 0.051	0.159ProCys: 0.159 ± 0.015	1.953ProAsp: 1.953 ± 0.079	2.755ProGlu: 2.755 ± 0.068	1.778ProPhe: 1.778 ± 0.057	1.957ProGly: 1.957 ± 0.066	0.855ProHis: 0.855 ± 0.032	2.899ProIle: 2.899 ± 0.082	2.463ProLys: 2.463 ± 0.06	3.092ProLeu: 3.092 ± 0.068	0.843ProMet: 0.843 ± 0.039	1.884ProAsn: 1.884 ± 0.077	0.791ProPro: 0.791 ± 0.045	1.099ProGln: 1.099 ± 0.043	0.96ProArg: 0.96 ± 0.037	2.141ProSer: 2.141 ± 0.079	2.013ProThr: 2.013 ± 0.081	2.467ProVal: 2.467 ± 0.09	0.272ProTrp: 0.272 ± 0.02	1.409ProTyr: 1.409 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.591GlnAla: 2.591 ± 0.077	0.16GlnCys: 0.16 ± 0.016	2.272GlnAsp: 2.272 ± 0.07	2.394GlnGlu: 2.394 ± 0.062	1.759GlnPhe: 1.759 ± 0.046	1.988GlnGly: 1.988 ± 0.058	0.99GlnHis: 0.99 ± 0.045	2.865GlnIle: 2.865 ± 0.064	2.583GlnLys: 2.583 ± 0.066	3.772GlnLeu: 3.772 ± 0.084	1.071GlnMet: 1.071 ± 0.044	1.985GlnAsn: 1.985 ± 0.07	1.117GlnPro: 1.117 ± 0.051	1.719GlnGln: 1.719 ± 0.075	1.419GlnArg: 1.419 ± 0.05	2.557GlnSer: 2.557 ± 0.078	2.242GlnThr: 2.242 ± 0.065	2.303GlnVal: 2.303 ± 0.064	0.266GlnTrp: 0.266 ± 0.022	1.539GlnTyr: 1.539 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	2.122ArgAla: 2.122 ± 0.063	0.19ArgCys: 0.19 ± 0.019	2.272ArgAsp: 2.272 ± 0.069	2.732ArgGlu: 2.732 ± 0.071	1.828ArgPhe: 1.828 ± 0.058	2.079ArgGly: 2.079 ± 0.065	0.979ArgHis: 0.979 ± 0.037	3.033ArgIle: 3.033 ± 0.068	2.865ArgLys: 2.865 ± 0.069	3.581ArgLeu: 3.581 ± 0.075	1.046ArgMet: 1.046 ± 0.043	1.918ArgAsn: 1.918 ± 0.063	1.181ArgPro: 1.181 ± 0.043	1.518ArgGln: 1.518 ± 0.054	1.731ArgArg: 1.731 ± 0.061	1.946ArgSer: 1.946 ± 0.059	2.091ArgThr: 2.091 ± 0.179	2.547ArgVal: 2.547 ± 0.061	0.248ArgTrp: 0.248 ± 0.018	1.678ArgTyr: 1.678 ± 0.056	0.003ArgXaa: 0.003 ± 0.003
Ser	3.103SerAla: 3.103 ± 0.08	0.367SerCys: 0.367 ± 0.025	3.497SerAsp: 3.497 ± 0.111	4.17SerGlu: 4.17 ± 0.107	3.096SerPhe: 3.096 ± 0.083	3.904SerGly: 3.904 ± 0.084	1.483SerHis: 1.483 ± 0.047	5.037SerIle: 5.037 ± 0.079	4.748SerLys: 4.748 ± 0.096	5.69SerLeu: 5.69 ± 0.09	1.677SerMet: 1.677 ± 0.05	3.225SerAsn: 3.225 ± 0.088	1.753SerPro: 1.753 ± 0.049	2.304SerGln: 2.304 ± 0.075	2.165SerArg: 2.165 ± 0.062	3.59SerSer: 3.59 ± 0.1	3.353SerThr: 3.353 ± 0.151	3.882SerVal: 3.882 ± 0.079	0.401SerTrp: 0.401 ± 0.031	2.491SerTyr: 2.491 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	3.25ThrAla: 3.25 ± 0.123	0.403ThrCys: 0.403 ± 0.027	3.143ThrAsp: 3.143 ± 0.142	3.391ThrGlu: 3.391 ± 0.102	3.036ThrPhe: 3.036 ± 0.082	3.79ThrGly: 3.79 ± 0.122	1.459ThrHis: 1.459 ± 0.051	4.77ThrIle: 4.77 ± 0.098	3.741ThrLys: 3.741 ± 0.078	5.822ThrLeu: 5.822 ± 0.08	1.421ThrMet: 1.421 ± 0.049	2.646ThrAsn: 2.646 ± 0.083	2.439ThrPro: 2.439 ± 0.123	1.712ThrGln: 1.712 ± 0.064	1.894ThrArg: 1.894 ± 0.055	3.691ThrSer: 3.691 ± 0.133	3.499ThrThr: 3.499 ± 0.197	4.073ThrVal: 4.073 ± 0.201	0.441ThrTrp: 0.441 ± 0.022	2.61ThrTyr: 2.61 ± 0.184	0.0ThrXaa: 0.0 ± 0.0
Val	4.344ValAla: 4.344 ± 0.099	0.533ValCys: 0.533 ± 0.028	4.322ValAsp: 4.322 ± 0.08	4.629ValGlu: 4.629 ± 0.097	2.845ValPhe: 2.845 ± 0.071	4.275ValGly: 4.275 ± 0.089	1.462ValHis: 1.462 ± 0.046	5.866ValIle: 5.866 ± 0.108	5.015ValLys: 5.015 ± 0.086	6.27ValLeu: 6.27 ± 0.111	1.787ValMet: 1.787 ± 0.052	3.449ValAsn: 3.449 ± 0.083	2.438ValPro: 2.438 ± 0.059	2.094ValGln: 2.094 ± 0.059	2.48ValArg: 2.48 ± 0.178	4.42ValSer: 4.42 ± 0.1	4.404ValThr: 4.404 ± 0.234	4.948ValVal: 4.948 ± 0.112	0.458ValTrp: 0.458 ± 0.026	2.326ValTyr: 2.326 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.364TrpAla: 0.364 ± 0.026	0.079TrpCys: 0.079 ± 0.014	0.379TrpAsp: 0.379 ± 0.026	0.364TrpGlu: 0.364 ± 0.025	0.426TrpPhe: 0.426 ± 0.03	0.425TrpGly: 0.425 ± 0.027	0.212TrpHis: 0.212 ± 0.017	0.605TrpIle: 0.605 ± 0.029	0.428TrpLys: 0.428 ± 0.028	0.942TrpLeu: 0.942 ± 0.036	0.26TrpMet: 0.26 ± 0.02	0.404TrpAsn: 0.404 ± 0.029	0.184TrpPro: 0.184 ± 0.016	0.27TrpGln: 0.27 ± 0.023	0.269TrpArg: 0.269 ± 0.021	0.448TrpSer: 0.448 ± 0.028	0.344TrpThr: 0.344 ± 0.021	0.456TrpVal: 0.456 ± 0.025	0.065TrpTrp: 0.065 ± 0.012	0.31TrpTyr: 0.31 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.254TyrAla: 2.254 ± 0.061	0.248TyrCys: 0.248 ± 0.02	2.739TyrAsp: 2.739 ± 0.063	2.714TyrGlu: 2.714 ± 0.066	1.884TyrPhe: 1.884 ± 0.053	2.407TyrGly: 2.407 ± 0.059	1.093TyrHis: 1.093 ± 0.044	2.902TyrIle: 2.902 ± 0.07	2.532TyrLys: 2.532 ± 0.067	3.622TyrLeu: 3.622 ± 0.085	0.907TyrMet: 0.907 ± 0.04	1.991TyrAsn: 1.991 ± 0.059	1.245TyrPro: 1.245 ± 0.047	1.916TyrGln: 1.916 ± 0.058	1.553TyrArg: 1.553 ± 0.056	1.919TyrSer: 1.919 ± 0.053	2.012TyrThr: 2.012 ± 0.05	2.498TyrVal: 2.498 ± 0.172	0.298TyrTrp: 0.298 ± 0.022	1.641TyrTyr: 1.641 ± 0.064	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.003XaaThr: 0.003 ± 0.003	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2323 proteins (680527 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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