Mycobacterium phage Bipolar

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097EVE5|A0A097EVE5_9CAUD Uncharacterized protein OS=Mycobacterium phage Bipolar OX=1551711 GN=PBI_BIPOLAR_46 PE=4 SV=1
MM1 pKa = 7.24MSADD5 pKa = 3.89PVRR8 pKa = 11.84GAIQASLDD16 pKa = 3.5AMGDD20 pKa = 3.44GWQVAHH26 pKa = 5.73YY27 pKa = 9.08VVVVGLEE34 pKa = 4.17RR35 pKa = 11.84IDD37 pKa = 4.1GDD39 pKa = 4.67RR40 pKa = 11.84MDD42 pKa = 5.5LGATTVITPIGQAGYY57 pKa = 8.35VTDD60 pKa = 4.2GLVNRR65 pKa = 11.84YY66 pKa = 7.46WDD68 pKa = 3.68EE69 pKa = 4.5SSDD72 pKa = 3.71EE73 pKa = 4.08

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097EVG2|A0A097EVG2_9CAUD Uncharacterized protein OS=Mycobacterium phage Bipolar OX=1551711 GN=PBI_BIPOLAR_49 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84PVFYY7 pKa = 10.83VDD9 pKa = 3.52QKK11 pKa = 11.59EE12 pKa = 4.07DD13 pKa = 3.67LEE15 pKa = 5.75KK16 pKa = 10.78GTFWWTVASSNGQVILTSEE35 pKa = 4.17MYY37 pKa = 10.08SRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84DD42 pKa = 3.14ARR44 pKa = 11.84KK45 pKa = 8.7AARR48 pKa = 11.84RR49 pKa = 11.84FITHH53 pKa = 4.63THH55 pKa = 4.4GMVVFRR61 pKa = 11.84YY62 pKa = 9.31HH63 pKa = 6.92DD64 pKa = 3.68RR65 pKa = 11.84NGEE68 pKa = 4.11RR69 pKa = 11.84VQSVLINWHH78 pKa = 5.85SFAVAAGGGGGGGGGGVSVGGVSVGGGGNGEE109 pKa = 4.19AFGKK113 pKa = 10.03GAGVRR118 pKa = 11.84FQQ120 pKa = 3.58

Molecular weight: 12.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

18870

30

1175

178.0

19.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.673 ± 0.455

1.182 ± 0.156

6.762 ± 0.247

5.946 ± 0.294

2.819 ± 0.151

8.728 ± 0.539

2.215 ± 0.171

4.388 ± 0.193

3.604 ± 0.208

7.324 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.146

3.19 ± 0.169

5.782 ± 0.178

3.498 ± 0.194

7.011 ± 0.362

5.734 ± 0.244

6.683 ± 0.268

7.419 ± 0.253

2.252 ± 0.165

2.501 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski