Eikenella longinqua
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2055 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A9RXB5|A0A1A9RXB5_9NEIS Transcriptional regulator OS=Eikenella longinqua OX=1795827 GN=A7P95_06515 PE=4 SV=1
MM1 pKa = 7.64 GLGNFYY7 pKa = 10.66 NQQLSLSEE15 pKa = 4.67 VIDD18 pKa = 3.9 CAKK21 pKa = 10.58 LFNNNDD27 pKa = 3.09 TDD29 pKa = 3.74 KK30 pKa = 10.76 FAKK33 pKa = 10.17 FPYY36 pKa = 9.88 LYY38 pKa = 10.76 SADD41 pKa = 4.05 EE42 pKa = 4.39 DD43 pKa = 4.05 SGPLKK48 pKa = 10.29 LTTSCYY54 pKa = 10.51 LSDD57 pKa = 3.76 SPEE60 pKa = 5.16 IIDD63 pKa = 4.2 KK64 pKa = 11.29 KK65 pKa = 10.55 EE66 pKa = 3.82 VFPKK70 pKa = 10.65 LVLEE74 pKa = 4.67 NGLSVLCSGEE84 pKa = 3.69 IFMDD88 pKa = 3.99 VYY90 pKa = 11.69 DD91 pKa = 5.35 DD92 pKa = 5.28 LDD94 pKa = 3.53 DD95 pKa = 5.45 RR96 pKa = 11.84 YY97 pKa = 10.69 PDD99 pKa = 3.44 FTKK102 pKa = 10.7 EE103 pKa = 3.69 QFIAALNYY111 pKa = 10.91 YY112 pKa = 8.89 MDD114 pKa = 4.43 NDD116 pKa = 4.43 DD117 pKa = 4.49 YY118 pKa = 11.82 IEE120 pKa = 3.92 FF121 pKa = 4.64
Molecular weight: 13.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.935
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A1A9S2T0|A0A1A9S2T0_9NEIS Uncharacterized protein OS=Eikenella longinqua OX=1795827 GN=A7P95_01475 PE=4 SV=1
MM1 pKa = 7.37 SWFLSRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 10.39 GYY12 pKa = 10.9 LKK14 pKa = 9.78 MGKK17 pKa = 10.04 ASFCKK22 pKa = 10.56 ANTIALLPVLLFALHH37 pKa = 6.23 AQAEE41 pKa = 4.7 TVSGEE46 pKa = 4.3 VVRR49 pKa = 11.84 VWDD52 pKa = 4.08 GDD54 pKa = 4.11 SLHH57 pKa = 7.03 LLDD60 pKa = 5.68 HH61 pKa = 6.96 AGQRR65 pKa = 11.84 HH66 pKa = 6.83 KK67 pKa = 10.85 IRR69 pKa = 11.84 LADD72 pKa = 3.2 IDD74 pKa = 4.32 APEE77 pKa = 4.62 LEE79 pKa = 4.36 QPQGRR84 pKa = 11.84 SCRR87 pKa = 11.84 NRR89 pKa = 11.84 LAEE92 pKa = 4.12 QVLHH96 pKa = 6.53 RR97 pKa = 11.84 RR98 pKa = 11.84 VQAEE102 pKa = 3.93 IVDD105 pKa = 4.05 TDD107 pKa = 3.27 PHH109 pKa = 6.0 GRR111 pKa = 11.84 QVAYY115 pKa = 9.86 IRR117 pKa = 11.84 LNGADD122 pKa = 3.67 INRR125 pKa = 11.84 QQIADD130 pKa = 3.67 GCAWHH135 pKa = 5.93 YY136 pKa = 11.02 RR137 pKa = 11.84 RR138 pKa = 11.84 FARR141 pKa = 11.84 QRR143 pKa = 11.84 QSPSAYY149 pKa = 9.39 AAYY152 pKa = 9.93 AAAEE156 pKa = 4.16 EE157 pKa = 4.7 GAKK160 pKa = 9.24 QRR162 pKa = 11.84 RR163 pKa = 11.84 AGVWRR168 pKa = 11.84 QDD170 pKa = 3.1 APQAPWQFRR179 pKa = 11.84 RR180 pKa = 11.84 QQRR183 pKa = 11.84 RR184 pKa = 11.84 PHH186 pKa = 5.68
Molecular weight: 21.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.604
IPC_protein 10.613
Toseland 10.599
ProMoST 10.423
Dawson 10.716
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.774
Grimsley 10.774
Solomon 10.877
Lehninger 10.833
Nozaki 10.599
DTASelect 10.482
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.467
IPC_peptide 10.877
IPC2_peptide 9.692
IPC2.peptide.svr19 8.557
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2055
0
2055
586508
41
2341
285.4
31.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.654 ± 0.089
1.048 ± 0.021
4.792 ± 0.04
5.872 ± 0.06
3.863 ± 0.04
7.626 ± 0.05
2.466 ± 0.029
5.237 ± 0.042
4.01 ± 0.047
10.765 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.027
3.505 ± 0.046
4.891 ± 0.04
4.947 ± 0.046
6.209 ± 0.047
5.327 ± 0.039
4.637 ± 0.041
6.516 ± 0.049
1.388 ± 0.027
2.852 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here