Roseivivax roseus
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4061 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9P807|A0A1H9P807_9RHOB Trk system potassium uptake protein TrkA OS=Roseivivax roseus OX=641238 GN=SAMN04490244_10159 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 10.44 RR3 pKa = 11.84 IATLTALGALIAGQAGAGGVEE24 pKa = 4.85 RR25 pKa = 11.84 SAQSAGILFEE35 pKa = 6.52 DD36 pKa = 4.54 GDD38 pKa = 4.14 RR39 pKa = 11.84 VEE41 pKa = 5.51 LSFGYY46 pKa = 10.31 VSPDD50 pKa = 3.06 VSGEE54 pKa = 3.7 QLLPVGPTSPAGASSGDD71 pKa = 3.37 MSEE74 pKa = 5.42 DD75 pKa = 3.57 YY76 pKa = 10.11 STLAFGYY83 pKa = 8.7 KK84 pKa = 9.01 QQVNEE89 pKa = 4.35 SVALALIVDD98 pKa = 4.12 NPIGADD104 pKa = 3.34 VNYY107 pKa = 10.77 GSDD110 pKa = 3.05 TQYY113 pKa = 11.36 LYY115 pKa = 11.45 GGGAPFAALGLNSSTAEE132 pKa = 3.76 IDD134 pKa = 3.74 SAAVTALASYY144 pKa = 10.12 KK145 pKa = 10.56 LPSNVTLFGGLRR157 pKa = 11.84 AQQASGGVSLFNGYY171 pKa = 8.36 EE172 pKa = 4.03 MEE174 pKa = 4.45 TSSEE178 pKa = 3.65 TDD180 pKa = 2.96 YY181 pKa = 11.62 GYY183 pKa = 11.61 VLGAAYY189 pKa = 7.81 EE190 pKa = 4.2 RR191 pKa = 11.84 PDD193 pKa = 2.95 IALRR197 pKa = 11.84 VALTYY202 pKa = 10.71 NSEE205 pKa = 3.88 ITHH208 pKa = 6.77 DD209 pKa = 4.72 FSASEE214 pKa = 4.36 TVFDD218 pKa = 4.85 PATQSVSNFDD228 pKa = 3.47 TSFEE232 pKa = 4.41 TTVPQSLNLEE242 pKa = 4.39 VQSGIAPDD250 pKa = 3.66 TLAFGSVRR258 pKa = 11.84 WVDD261 pKa = 2.97 WSEE264 pKa = 3.69 FDD266 pKa = 3.7 ITPSVYY272 pKa = 8.49 NTAYY276 pKa = 10.62 NGSLVNYY283 pKa = 9.46 DD284 pKa = 4.58 DD285 pKa = 6.02 DD286 pKa = 5.25 VITYY290 pKa = 7.77 TVGLGRR296 pKa = 11.84 RR297 pKa = 11.84 FTEE300 pKa = 3.78 NWSGAVLATYY310 pKa = 10.06 EE311 pKa = 4.12 DD312 pKa = 4.07 QNGGFSGNLGPTDD325 pKa = 3.68 GQTSVGVAVTYY336 pKa = 7.39 EE337 pKa = 3.99 TEE339 pKa = 4.07 RR340 pKa = 11.84 YY341 pKa = 8.76 EE342 pKa = 3.73 ITGGLRR348 pKa = 11.84 HH349 pKa = 6.04 IWIGDD354 pKa = 3.24 ARR356 pKa = 11.84 TEE358 pKa = 4.12 APSALDD364 pKa = 3.52 QPAGTAFGDD373 pKa = 3.86 FDD375 pKa = 6.37 DD376 pKa = 4.34 NTAIAGGLQVAIKK389 pKa = 9.78 FF390 pKa = 3.92
Molecular weight: 40.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.859
IPC_protein 3.859
Toseland 3.643
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.973
Patrickios 0.973
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A1H9XBE8|A0A1H9XBE8_9RHOB HNH endonuclease OS=Roseivivax roseus OX=641238 GN=SAMN04490244_1311 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 AGRR28 pKa = 11.84 KK29 pKa = 8.43 IVNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 6.6 TVSAA44 pKa = 4.51
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4061
0
4061
1273032
27
4772
313.5
33.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.716 ± 0.06
0.848 ± 0.015
6.399 ± 0.039
6.201 ± 0.041
3.539 ± 0.028
9.032 ± 0.045
2.0 ± 0.02
4.683 ± 0.03
2.365 ± 0.034
10.08 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.638 ± 0.019
2.221 ± 0.021
5.26 ± 0.027
2.938 ± 0.026
7.418 ± 0.053
5.076 ± 0.026
5.581 ± 0.027
7.479 ± 0.03
1.414 ± 0.018
2.111 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here