Salinibacterium sp. dk2585

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Salinibacterium; unclassified Salinibacterium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9G5A0|A0A5B9G5A0_9MICO Uncharacterized protein OS=Salinibacterium sp. dk2585 OX=2603292 GN=FVA74_13220 PE=4 SV=1
MM1 pKa = 6.98HH2 pKa = 6.88TRR4 pKa = 11.84TRR6 pKa = 11.84RR7 pKa = 11.84QRR9 pKa = 11.84RR10 pKa = 11.84LGAVAGTALGAMLVTGCVASEE31 pKa = 4.01RR32 pKa = 11.84GEE34 pKa = 4.25DD35 pKa = 3.53SGDD38 pKa = 3.41AAGTFVFAASAEE50 pKa = 4.22PVTLDD55 pKa = 3.83PAFANDD61 pKa = 3.55GEE63 pKa = 4.63TFRR66 pKa = 11.84VARR69 pKa = 11.84QIFEE73 pKa = 3.94GLIGVEE79 pKa = 4.14QGSADD84 pKa = 4.05PAPLLAEE91 pKa = 4.07SWEE94 pKa = 4.31GSDD97 pKa = 5.84DD98 pKa = 3.74GLSYY102 pKa = 10.95TFDD105 pKa = 3.45LKK107 pKa = 11.17EE108 pKa = 3.97GVTFHH113 pKa = 7.87DD114 pKa = 3.63GTEE117 pKa = 4.32FNAEE121 pKa = 3.9AVCYY125 pKa = 10.81NFDD128 pKa = 3.21RR129 pKa = 11.84WHH131 pKa = 5.96NFEE134 pKa = 6.08GIAQSPSASYY144 pKa = 10.92YY145 pKa = 10.31YY146 pKa = 11.33NNLFKK151 pKa = 11.09GFAGDD156 pKa = 3.23EE157 pKa = 4.27GAVYY161 pKa = 9.18EE162 pKa = 4.44SCEE165 pKa = 3.86APSEE169 pKa = 4.19NEE171 pKa = 4.3AIVHH175 pKa = 6.03LTQPWAGFIAALSLPSFSMQSPTALQEE202 pKa = 3.9YY203 pKa = 9.62GADD206 pKa = 4.01DD207 pKa = 3.17IGGTEE212 pKa = 4.05EE213 pKa = 4.3APSISEE219 pKa = 4.21YY220 pKa = 10.93GSEE223 pKa = 4.46HH224 pKa = 6.0PTGTGPFTFSSWDD237 pKa = 3.1AGSQVEE243 pKa = 4.19LTAYY247 pKa = 10.33DD248 pKa = 4.69DD249 pKa = 3.73YY250 pKa = 11.13WGEE253 pKa = 4.05QGDD256 pKa = 3.9VTDD259 pKa = 4.14VIFRR263 pKa = 11.84VIDD266 pKa = 3.86DD267 pKa = 3.94PNARR271 pKa = 11.84RR272 pKa = 11.84QALEE276 pKa = 3.88AGTIDD281 pKa = 4.8GYY283 pKa = 11.41DD284 pKa = 3.47LVSPADD290 pKa = 3.64TAALEE295 pKa = 4.06EE296 pKa = 5.29DD297 pKa = 4.15GFQILARR304 pKa = 11.84DD305 pKa = 4.04PFTILYY311 pKa = 10.2LGINQAVPEE320 pKa = 4.29LQDD323 pKa = 3.1VRR325 pKa = 11.84VRR327 pKa = 11.84QAISMAIDD335 pKa = 3.34KK336 pKa = 9.24EE337 pKa = 4.22QLVNQTLPEE346 pKa = 4.23GTEE349 pKa = 3.95LATQFIPPVVSGYY362 pKa = 10.78NEE364 pKa = 4.65DD365 pKa = 3.64VTTYY369 pKa = 10.85DD370 pKa = 3.74YY371 pKa = 11.72DD372 pKa = 3.61VDD374 pKa = 3.7AAKK377 pKa = 10.84ALLAEE382 pKa = 4.54AGIPEE387 pKa = 4.18GWEE390 pKa = 3.62LTFNYY395 pKa = 6.61PTNVSRR401 pKa = 11.84PYY403 pKa = 9.52MPNPEE408 pKa = 4.49EE409 pKa = 4.14IFTVLSTQLSEE420 pKa = 3.85IGITVVPQANEE431 pKa = 3.26WGEE434 pKa = 4.1YY435 pKa = 9.71LDD437 pKa = 4.58IMQGSSDD444 pKa = 3.79HH445 pKa = 6.87GIHH448 pKa = 6.59LLGWTGDD455 pKa = 3.85YY456 pKa = 11.17NDD458 pKa = 3.83TDD460 pKa = 3.59NFVGVFFGQPSMEE473 pKa = 4.23FGFDD477 pKa = 3.3NPEE480 pKa = 3.99LFNALTEE487 pKa = 4.28ARR489 pKa = 11.84GTATIEE495 pKa = 4.09EE496 pKa = 4.23QLPLYY501 pKa = 10.56EE502 pKa = 6.2DD503 pKa = 3.77INQMIAEE510 pKa = 4.71YY511 pKa = 10.32IPAVPLAHH519 pKa = 7.21PAPSLAFSEE528 pKa = 4.98RR529 pKa = 11.84VGSYY533 pKa = 9.65PVSPVNDD540 pKa = 3.14EE541 pKa = 4.23VYY543 pKa = 11.31NLVEE547 pKa = 4.82LNDD550 pKa = 3.62

Molecular weight: 59.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9G5D1|A0A5B9G5D1_9MICO SDR family oxidoreductase OS=Salinibacterium sp. dk2585 OX=2603292 GN=FVA74_05045 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84AITFATGTSVAGRR15 pKa = 11.84LTLRR19 pKa = 11.84APRR22 pKa = 11.84TIVVTQRR29 pKa = 11.84PSVIASTSGVASPGGVAPRR48 pKa = 11.84GLAATRR54 pKa = 11.84FPFRR58 pKa = 11.84GAAVVIASAVRR69 pKa = 11.84ALTWSAGTII78 pKa = 3.63

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2617

0

2617

860142

24

2600

328.7

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.903 ± 0.063

0.503 ± 0.011

5.809 ± 0.039

6.207 ± 0.055

3.169 ± 0.03

8.822 ± 0.043

2.005 ± 0.021

4.758 ± 0.042

2.21 ± 0.035

10.147 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.021

2.145 ± 0.024

5.204 ± 0.032

2.826 ± 0.024

7.071 ± 0.059

6.026 ± 0.035

5.916 ± 0.054

8.929 ± 0.047

1.406 ± 0.022

1.993 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski