Amino acid dipepetide frequency for Lactococcus phage CHPC967

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.299AlaAla: 0.299 ± 0.198
0.15AlaCys: 0.15 ± 0.159
2.693AlaAsp: 2.693 ± 0.586
5.535AlaGlu: 5.535 ± 1.136
2.244AlaPhe: 2.244 ± 0.477
3.141AlaGly: 3.141 ± 0.72
0.15AlaHis: 0.15 ± 0.146
4.188AlaIle: 4.188 ± 0.793
5.086AlaLys: 5.086 ± 0.837
6.432AlaLeu: 6.432 ± 1.455
1.496AlaMet: 1.496 ± 0.337
4.936AlaAsn: 4.936 ± 0.983
1.197AlaPro: 1.197 ± 0.373
2.992AlaGln: 2.992 ± 0.793
1.795AlaArg: 1.795 ± 0.614
2.992AlaSer: 2.992 ± 0.655
4.039AlaThr: 4.039 ± 1.304
3.889AlaVal: 3.889 ± 1.233
1.047AlaTrp: 1.047 ± 0.384
3.141AlaTyr: 3.141 ± 0.521
0.0AlaXaa: 0.0 ± 0.0
Cys
0.15CysAla: 0.15 ± 0.159
0.15CysCys: 0.15 ± 0.178
0.299CysAsp: 0.299 ± 0.196
0.299CysGlu: 0.299 ± 0.208
0.299CysPhe: 0.299 ± 0.233
0.449CysGly: 0.449 ± 0.229
0.449CysHis: 0.449 ± 0.548
0.15CysIle: 0.15 ± 0.173
1.197CysLys: 1.197 ± 0.351
0.748CysLeu: 0.748 ± 0.497
0.0CysMet: 0.0 ± 0.0
0.299CysAsn: 0.299 ± 0.199
0.299CysPro: 0.299 ± 0.2
0.598CysGln: 0.598 ± 0.289
0.449CysArg: 0.449 ± 0.248
0.748CysSer: 0.748 ± 0.263
0.748CysThr: 0.748 ± 0.445
0.299CysVal: 0.299 ± 0.182
0.299CysTrp: 0.299 ± 0.207
0.449CysTyr: 0.449 ± 0.23
0.0CysXaa: 0.0 ± 0.0
Asp
2.094AspAla: 2.094 ± 0.632
0.449AspCys: 0.449 ± 0.235
4.039AspAsp: 4.039 ± 0.804
5.385AspGlu: 5.385 ± 1.269
4.637AspPhe: 4.637 ± 0.675
5.236AspGly: 5.236 ± 1.448
0.0AspHis: 0.0 ± 0.0
2.992AspIle: 2.992 ± 0.531
4.936AspLys: 4.936 ± 0.752
4.787AspLeu: 4.787 ± 0.888
2.244AspMet: 2.244 ± 0.653
3.59AspAsn: 3.59 ± 0.677
1.795AspPro: 1.795 ± 0.55
0.449AspGln: 0.449 ± 0.25
1.197AspArg: 1.197 ± 0.392
3.441AspSer: 3.441 ± 0.488
4.637AspThr: 4.637 ± 0.705
3.141AspVal: 3.141 ± 0.814
0.898AspTrp: 0.898 ± 0.337
3.59AspTyr: 3.59 ± 0.952
0.0AspXaa: 0.0 ± 0.0
Glu
4.488GluAla: 4.488 ± 0.795
1.047GluCys: 1.047 ± 0.375
2.842GluAsp: 2.842 ± 0.54
7.031GluGlu: 7.031 ± 1.535
4.637GluPhe: 4.637 ± 0.84
2.693GluGly: 2.693 ± 0.607
2.244GluHis: 2.244 ± 0.874
3.74GluIle: 3.74 ± 0.812
4.338GluLys: 4.338 ± 1.031
7.031GluLeu: 7.031 ± 1.117
4.188GluMet: 4.188 ± 0.807
3.59GluAsn: 3.59 ± 0.726
1.047GluPro: 1.047 ± 0.343
5.086GluGln: 5.086 ± 0.777
2.992GluArg: 2.992 ± 0.536
2.693GluSer: 2.693 ± 0.549
5.236GluThr: 5.236 ± 0.882
5.535GluVal: 5.535 ± 0.998
0.748GluTrp: 0.748 ± 0.247
2.244GluTyr: 2.244 ± 0.681
0.0GluXaa: 0.0 ± 0.0
Phe
1.945PheAla: 1.945 ± 0.475
0.748PheCys: 0.748 ± 0.269
2.543PheAsp: 2.543 ± 0.45
2.992PheGlu: 2.992 ± 0.609
1.197PhePhe: 1.197 ± 0.346
3.441PheGly: 3.441 ± 0.534
0.299PheHis: 0.299 ± 0.185
4.488PheIle: 4.488 ± 0.993
4.787PheLys: 4.787 ± 1.007
2.693PheLeu: 2.693 ± 0.634
1.197PheMet: 1.197 ± 0.328
4.488PheAsn: 4.488 ± 0.717
1.496PhePro: 1.496 ± 0.597
0.748PheGln: 0.748 ± 0.232
1.795PheArg: 1.795 ± 0.422
3.441PheSer: 3.441 ± 0.873
2.244PheThr: 2.244 ± 0.54
2.244PheVal: 2.244 ± 0.727
0.748PheTrp: 0.748 ± 0.295
2.244PheTyr: 2.244 ± 0.532
0.0PheXaa: 0.0 ± 0.0
Gly
4.039GlyAla: 4.039 ± 1.132
0.299GlyCys: 0.299 ± 0.25
3.74GlyAsp: 3.74 ± 0.988
3.141GlyGlu: 3.141 ± 0.771
4.039GlyPhe: 4.039 ± 0.879
5.385GlyGly: 5.385 ± 0.87
0.748GlyHis: 0.748 ± 0.339
4.936GlyIle: 4.936 ± 0.914
4.338GlyLys: 4.338 ± 0.878
5.385GlyLeu: 5.385 ± 0.607
2.244GlyMet: 2.244 ± 0.612
2.693GlyAsn: 2.693 ± 0.573
0.299GlyPro: 0.299 ± 0.181
2.693GlyGln: 2.693 ± 0.591
3.441GlyArg: 3.441 ± 0.689
5.984GlySer: 5.984 ± 1.342
5.684GlyThr: 5.684 ± 1.19
4.787GlyVal: 4.787 ± 0.944
0.898GlyTrp: 0.898 ± 0.358
3.74GlyTyr: 3.74 ± 0.76
0.0GlyXaa: 0.0 ± 0.0
His
0.748HisAla: 0.748 ± 0.336
0.299HisCys: 0.299 ± 0.228
1.047HisAsp: 1.047 ± 0.285
0.449HisGlu: 0.449 ± 0.26
0.898HisPhe: 0.898 ± 0.38
0.449HisGly: 0.449 ± 0.227
0.299HisHis: 0.299 ± 0.228
1.047HisIle: 1.047 ± 0.289
1.197HisLys: 1.197 ± 0.61
1.645HisLeu: 1.645 ± 0.515
0.15HisMet: 0.15 ± 0.146
0.898HisAsn: 0.898 ± 0.432
0.15HisPro: 0.15 ± 0.14
0.449HisGln: 0.449 ± 0.246
0.449HisArg: 0.449 ± 0.263
0.748HisSer: 0.748 ± 0.433
0.449HisThr: 0.449 ± 0.266
0.598HisVal: 0.598 ± 0.355
0.598HisTrp: 0.598 ± 0.291
1.047HisTyr: 1.047 ± 0.453
0.15HisXaa: 0.15 ± 0.16
Ile
3.441IleAla: 3.441 ± 0.62
0.598IleCys: 0.598 ± 0.271
4.936IleAsp: 4.936 ± 0.857
6.582IleGlu: 6.582 ± 0.736
2.094IlePhe: 2.094 ± 0.496
5.086IleGly: 5.086 ± 0.99
1.047IleHis: 1.047 ± 0.414
4.188IleIle: 4.188 ± 0.937
6.731IleLys: 6.731 ± 1.118
4.338IleLeu: 4.338 ± 0.732
1.496IleMet: 1.496 ± 0.563
5.086IleAsn: 5.086 ± 0.833
1.945IlePro: 1.945 ± 0.439
1.945IleGln: 1.945 ± 0.436
1.795IleArg: 1.795 ± 0.536
2.992IleSer: 2.992 ± 0.658
3.441IleThr: 3.441 ± 0.853
2.693IleVal: 2.693 ± 0.508
0.898IleTrp: 0.898 ± 0.372
2.543IleTyr: 2.543 ± 0.529
0.0IleXaa: 0.0 ± 0.0
Lys
8.078LysAla: 8.078 ± 0.977
1.197LysCys: 1.197 ± 0.467
4.787LysAsp: 4.787 ± 0.953
7.629LysGlu: 7.629 ± 1.46
2.543LysPhe: 2.543 ± 0.547
6.283LysGly: 6.283 ± 1.071
1.346LysHis: 1.346 ± 0.439
5.684LysIle: 5.684 ± 1.264
7.031LysLys: 7.031 ± 0.902
6.731LysLeu: 6.731 ± 1.135
3.141LysMet: 3.141 ± 0.583
5.834LysAsn: 5.834 ± 0.786
2.842LysPro: 2.842 ± 0.759
4.787LysGln: 4.787 ± 0.603
2.842LysArg: 2.842 ± 0.498
3.441LysSer: 3.441 ± 0.502
4.338LysThr: 4.338 ± 1.004
6.283LysVal: 6.283 ± 0.989
1.346LysTrp: 1.346 ± 0.438
2.842LysTyr: 2.842 ± 0.668
0.0LysXaa: 0.0 ± 0.0
Leu
3.889LeuAla: 3.889 ± 0.778
1.197LeuCys: 1.197 ± 0.384
4.188LeuAsp: 4.188 ± 0.779
6.133LeuGlu: 6.133 ± 0.833
4.039LeuPhe: 4.039 ± 0.801
6.283LeuGly: 6.283 ± 1.671
1.645LeuHis: 1.645 ± 0.56
5.684LeuIle: 5.684 ± 1.11
7.33LeuLys: 7.33 ± 0.976
6.582LeuLeu: 6.582 ± 1.128
2.693LeuMet: 2.693 ± 0.932
4.188LeuAsn: 4.188 ± 0.766
2.244LeuPro: 2.244 ± 0.569
3.141LeuGln: 3.141 ± 0.697
2.244LeuArg: 2.244 ± 0.638
5.236LeuSer: 5.236 ± 1.171
5.086LeuThr: 5.086 ± 0.694
5.684LeuVal: 5.684 ± 1.171
0.449LeuTrp: 0.449 ± 0.27
3.291LeuTyr: 3.291 ± 0.783
0.0LeuXaa: 0.0 ± 0.0
Met
3.74MetAla: 3.74 ± 0.633
0.0MetCys: 0.0 ± 0.0
1.645MetAsp: 1.645 ± 0.478
1.945MetGlu: 1.945 ± 0.556
1.047MetPhe: 1.047 ± 0.495
1.645MetGly: 1.645 ± 0.385
0.0MetHis: 0.0 ± 0.0
2.094MetIle: 2.094 ± 0.583
2.992MetLys: 2.992 ± 0.571
3.141MetLeu: 3.141 ± 0.742
0.449MetMet: 0.449 ± 0.247
1.645MetAsn: 1.645 ± 0.529
0.299MetPro: 0.299 ± 0.27
0.748MetGln: 0.748 ± 0.366
0.748MetArg: 0.748 ± 0.241
2.094MetSer: 2.094 ± 0.501
1.346MetThr: 1.346 ± 0.448
1.496MetVal: 1.496 ± 0.468
0.15MetTrp: 0.15 ± 0.161
1.047MetTyr: 1.047 ± 0.363
0.0MetXaa: 0.0 ± 0.0
Asn
4.188AsnAla: 4.188 ± 0.983
0.299AsnCys: 0.299 ± 0.177
3.291AsnAsp: 3.291 ± 1.055
5.535AsnGlu: 5.535 ± 0.799
2.842AsnPhe: 2.842 ± 0.53
4.488AsnGly: 4.488 ± 0.695
0.449AsnHis: 0.449 ± 0.216
3.889AsnIle: 3.889 ± 0.792
5.236AsnLys: 5.236 ± 0.859
4.488AsnLeu: 4.488 ± 0.745
1.496AsnMet: 1.496 ± 0.355
3.74AsnAsn: 3.74 ± 0.609
2.693AsnPro: 2.693 ± 0.803
1.346AsnGln: 1.346 ± 0.445
2.094AsnArg: 2.094 ± 0.517
3.291AsnSer: 3.291 ± 0.776
4.936AsnThr: 4.936 ± 0.998
4.188AsnVal: 4.188 ± 0.612
1.047AsnTrp: 1.047 ± 0.339
2.992AsnTyr: 2.992 ± 0.75
0.0AsnXaa: 0.0 ± 0.0
Pro
0.898ProAla: 0.898 ± 0.444
0.0ProCys: 0.0 ± 0.0
1.795ProAsp: 1.795 ± 0.451
1.047ProGlu: 1.047 ± 0.404
1.496ProPhe: 1.496 ± 0.527
0.299ProGly: 0.299 ± 0.219
0.299ProHis: 0.299 ± 0.222
1.197ProIle: 1.197 ± 0.418
3.141ProLys: 3.141 ± 0.657
2.992ProLeu: 2.992 ± 0.519
0.0ProMet: 0.0 ± 0.0
2.094ProAsn: 2.094 ± 0.678
0.898ProPro: 0.898 ± 0.294
1.645ProGln: 1.645 ± 0.635
0.898ProArg: 0.898 ± 0.313
1.945ProSer: 1.945 ± 0.523
2.244ProThr: 2.244 ± 0.518
1.645ProVal: 1.645 ± 0.524
0.15ProTrp: 0.15 ± 0.16
1.047ProTyr: 1.047 ± 0.433
0.299ProXaa: 0.299 ± 0.321
Gln
2.842GlnAla: 2.842 ± 0.828
0.299GlnCys: 0.299 ± 0.188
2.543GlnAsp: 2.543 ± 0.753
1.496GlnGlu: 1.496 ± 0.504
1.197GlnPhe: 1.197 ± 0.584
2.393GlnGly: 2.393 ± 0.527
0.449GlnHis: 0.449 ± 0.234
2.543GlnIle: 2.543 ± 0.45
4.188GlnLys: 4.188 ± 0.915
2.992GlnLeu: 2.992 ± 0.796
1.346GlnMet: 1.346 ± 0.415
2.393GlnAsn: 2.393 ± 0.607
0.898GlnPro: 0.898 ± 0.306
2.094GlnGln: 2.094 ± 0.615
1.645GlnArg: 1.645 ± 0.511
2.244GlnSer: 2.244 ± 0.557
2.543GlnThr: 2.543 ± 0.556
2.842GlnVal: 2.842 ± 0.53
0.449GlnTrp: 0.449 ± 0.26
2.094GlnTyr: 2.094 ± 0.655
0.0GlnXaa: 0.0 ± 0.0
Arg
2.393ArgAla: 2.393 ± 0.729
0.15ArgCys: 0.15 ± 0.143
1.945ArgAsp: 1.945 ± 0.466
4.637ArgGlu: 4.637 ± 1.173
1.047ArgPhe: 1.047 ± 0.409
2.094ArgGly: 2.094 ± 0.503
0.598ArgHis: 0.598 ± 0.275
1.197ArgIle: 1.197 ± 0.352
3.441ArgLys: 3.441 ± 0.749
2.393ArgLeu: 2.393 ± 0.544
1.047ArgMet: 1.047 ± 0.411
1.645ArgAsn: 1.645 ± 0.55
1.346ArgPro: 1.346 ± 0.581
0.898ArgGln: 0.898 ± 0.27
1.346ArgArg: 1.346 ± 0.6
2.094ArgSer: 2.094 ± 0.654
1.795ArgThr: 1.795 ± 0.501
1.945ArgVal: 1.945 ± 0.586
0.598ArgTrp: 0.598 ± 0.312
1.197ArgTyr: 1.197 ± 0.415
0.0ArgXaa: 0.0 ± 0.0
Ser
3.291SerAla: 3.291 ± 0.913
0.0SerCys: 0.0 ± 0.0
3.74SerAsp: 3.74 ± 0.647
2.992SerGlu: 2.992 ± 0.732
2.842SerPhe: 2.842 ± 0.686
4.787SerGly: 4.787 ± 0.858
1.047SerHis: 1.047 ± 0.385
3.141SerIle: 3.141 ± 0.614
5.984SerLys: 5.984 ± 0.912
6.133SerLeu: 6.133 ± 0.792
0.748SerMet: 0.748 ± 0.308
3.441SerAsn: 3.441 ± 0.604
1.047SerPro: 1.047 ± 0.375
1.795SerGln: 1.795 ± 0.745
1.496SerArg: 1.496 ± 0.63
4.637SerSer: 4.637 ± 0.94
4.488SerThr: 4.488 ± 1.029
4.188SerVal: 4.188 ± 0.789
1.197SerTrp: 1.197 ± 0.462
2.393SerTyr: 2.393 ± 0.582
0.0SerXaa: 0.0 ± 0.0
Thr
3.889ThrAla: 3.889 ± 0.603
0.0ThrCys: 0.0 ± 0.0
5.086ThrAsp: 5.086 ± 1.062
3.74ThrGlu: 3.74 ± 0.897
3.74ThrPhe: 3.74 ± 0.621
5.984ThrGly: 5.984 ± 1.163
0.449ThrHis: 0.449 ± 0.262
5.834ThrIle: 5.834 ± 0.821
6.582ThrLys: 6.582 ± 0.873
5.684ThrLeu: 5.684 ± 0.967
1.047ThrMet: 1.047 ± 0.423
2.842ThrAsn: 2.842 ± 0.694
1.795ThrPro: 1.795 ± 0.653
2.842ThrGln: 2.842 ± 0.822
1.496ThrArg: 1.496 ± 0.386
3.889ThrSer: 3.889 ± 1.199
3.441ThrThr: 3.441 ± 0.818
3.889ThrVal: 3.889 ± 0.775
1.047ThrTrp: 1.047 ± 0.363
3.441ThrTyr: 3.441 ± 0.774
0.0ThrXaa: 0.0 ± 0.0
Val
2.842ValAla: 2.842 ± 0.579
0.299ValCys: 0.299 ± 0.208
4.637ValAsp: 4.637 ± 0.768
3.74ValGlu: 3.74 ± 0.52
2.393ValPhe: 2.393 ± 0.617
2.693ValGly: 2.693 ± 0.848
0.898ValHis: 0.898 ± 0.309
3.889ValIle: 3.889 ± 0.889
4.637ValLys: 4.637 ± 0.646
3.889ValLeu: 3.889 ± 0.835
1.795ValMet: 1.795 ± 0.479
4.338ValAsn: 4.338 ± 1.018
2.244ValPro: 2.244 ± 0.706
2.543ValGln: 2.543 ± 0.877
3.441ValArg: 3.441 ± 0.622
4.338ValSer: 4.338 ± 0.984
5.086ValThr: 5.086 ± 0.745
2.393ValVal: 2.393 ± 0.624
1.496ValTrp: 1.496 ± 0.426
3.291ValTyr: 3.291 ± 0.749
0.0ValXaa: 0.0 ± 0.0
Trp
0.898TrpAla: 0.898 ± 0.321
0.449TrpCys: 0.449 ± 0.223
0.898TrpAsp: 0.898 ± 0.348
0.598TrpGlu: 0.598 ± 0.315
1.047TrpPhe: 1.047 ± 0.421
1.346TrpGly: 1.346 ± 0.393
0.299TrpHis: 0.299 ± 0.238
0.449TrpIle: 0.449 ± 0.287
1.047TrpLys: 1.047 ± 0.376
1.795TrpLeu: 1.795 ± 0.369
0.15TrpMet: 0.15 ± 0.135
1.197TrpAsn: 1.197 ± 0.579
0.0TrpPro: 0.0 ± 0.0
1.047TrpGln: 1.047 ± 0.496
0.449TrpArg: 0.449 ± 0.26
0.748TrpSer: 0.748 ± 0.448
1.346TrpThr: 1.346 ± 0.394
0.898TrpVal: 0.898 ± 0.348
0.299TrpTrp: 0.299 ± 0.201
0.299TrpTyr: 0.299 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.291TyrAla: 3.291 ± 0.533
0.748TyrCys: 0.748 ± 0.257
2.842TyrAsp: 2.842 ± 0.508
2.543TyrGlu: 2.543 ± 0.62
1.496TyrPhe: 1.496 ± 0.485
4.488TyrGly: 4.488 ± 0.979
1.047TyrHis: 1.047 ± 0.488
2.543TyrIle: 2.543 ± 0.535
4.637TyrLys: 4.637 ± 0.826
1.346TyrLeu: 1.346 ± 0.606
1.197TyrMet: 1.197 ± 0.396
3.59TyrAsn: 3.59 ± 0.766
1.197TyrPro: 1.197 ± 0.499
1.645TyrGln: 1.645 ± 0.565
1.047TyrArg: 1.047 ± 0.451
2.393TyrSer: 2.393 ± 0.756
3.59TyrThr: 3.59 ± 0.769
2.244TyrVal: 2.244 ± 0.553
0.898TyrTrp: 0.898 ± 0.384
3.141TyrTyr: 3.141 ± 0.657
0.15TyrXaa: 0.15 ± 0.147
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.15XaaLys: 0.15 ± 0.16
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.299XaaPro: 0.299 ± 0.321
0.0XaaGln: 0.0 ± 0.0
0.15XaaArg: 0.15 ± 0.147
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (6686 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski