Clostridium niameyense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0RAR9|A0A6M0RAR9_9CLOT MATE family efflux transporter OS=Clostridium niameyense OX=1622073 GN=FDF74_09165 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 9.88STISKK7 pKa = 9.17VAYY10 pKa = 10.24LRR12 pKa = 11.84GLMDD16 pKa = 3.86GLEE19 pKa = 4.14INKK22 pKa = 8.52DD23 pKa = 3.48TKK25 pKa = 10.11EE26 pKa = 3.86GRR28 pKa = 11.84IILEE32 pKa = 4.38MINILDD38 pKa = 3.84SLAEE42 pKa = 3.92EE43 pKa = 4.28VNDD46 pKa = 3.47IKK48 pKa = 10.89EE49 pKa = 4.35SQSILQEE56 pKa = 4.0YY57 pKa = 10.0VEE59 pKa = 4.99TIDD62 pKa = 5.69ADD64 pKa = 4.17LLSLQEE70 pKa = 4.26NIYY73 pKa = 11.15DD74 pKa = 3.84EE75 pKa = 4.97DD76 pKa = 4.63CEE78 pKa = 4.63EE79 pKa = 4.23EE80 pKa = 5.32DD81 pKa = 4.6FLEE84 pKa = 4.54DD85 pKa = 5.04FIGVEE90 pKa = 4.44CPNCNEE96 pKa = 4.42TIYY99 pKa = 10.44IDD101 pKa = 4.15KK102 pKa = 10.77DD103 pKa = 3.33ALKK106 pKa = 11.13DD107 pKa = 3.35MTKK110 pKa = 9.84IFCPSCHH117 pKa = 6.03EE118 pKa = 4.65NISLEE123 pKa = 4.21DD124 pKa = 3.82FCSCDD129 pKa = 3.7YY130 pKa = 10.88IKK132 pKa = 10.95HH133 pKa = 6.25EE134 pKa = 4.36NTNNDD139 pKa = 2.82

Molecular weight: 16.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0RDU8|A0A6M0RDU8_9CLOT Phage major capsid protein OS=Clostridium niameyense OX=1622073 GN=FDF74_11415 PE=4 SV=1
MM1 pKa = 7.46GARR4 pKa = 11.84EE5 pKa = 3.82GGRR8 pKa = 11.84RR9 pKa = 11.84QRR11 pKa = 11.84RR12 pKa = 11.84PKK14 pKa = 10.5KK15 pKa = 9.82KK16 pKa = 9.29VCAFCIEE23 pKa = 3.98KK24 pKa = 10.64AGAIDD29 pKa = 3.58YY30 pKa = 11.18KK31 pKa = 11.02DD32 pKa = 3.2INKK35 pKa = 9.07LRR37 pKa = 11.84KK38 pKa = 9.24YY39 pKa = 9.06VTDD42 pKa = 3.62RR43 pKa = 11.84GKK45 pKa = 10.06ILPRR49 pKa = 11.84RR50 pKa = 11.84ISGNCAKK57 pKa = 10.14HH58 pKa = 5.4QRR60 pKa = 11.84EE61 pKa = 3.92LTIAIKK67 pKa = 10.21RR68 pKa = 11.84ARR70 pKa = 11.84NIALLPFTTEE80 pKa = 3.59

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2446

0

2446

738339

23

2880

301.9

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.119 ± 0.059

1.276 ± 0.021

5.353 ± 0.039

7.203 ± 0.066

4.29 ± 0.041

6.149 ± 0.054

1.287 ± 0.017

10.554 ± 0.057

10.206 ± 0.064

8.965 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.026

6.862 ± 0.058

2.772 ± 0.028

2.257 ± 0.026

3.211 ± 0.039

6.199 ± 0.052

4.753 ± 0.044

6.231 ± 0.045

0.604 ± 0.013

4.152 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski