Amino acid dipepetide frequency for Nocardioides sp. Root190

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.015AlaAla: 19.015 ± 0.197	0.965AlaCys: 0.965 ± 0.027	8.56AlaAsp: 8.56 ± 0.084	8.145AlaGlu: 8.145 ± 0.108	3.562AlaPhe: 3.562 ± 0.051	12.095AlaGly: 12.095 ± 0.099	2.532AlaHis: 2.532 ± 0.041	4.951AlaIle: 4.951 ± 0.067	2.641AlaLys: 2.641 ± 0.055	13.289AlaLeu: 13.289 ± 0.105	2.689AlaMet: 2.689 ± 0.049	2.138AlaAsn: 2.138 ± 0.039	6.233AlaPro: 6.233 ± 0.084	3.47AlaGln: 3.47 ± 0.048	8.989AlaArg: 8.989 ± 0.104	6.811AlaSer: 6.811 ± 0.066	7.945AlaThr: 7.945 ± 0.093	11.674AlaVal: 11.674 ± 0.106	1.959AlaTrp: 1.959 ± 0.039	2.275AlaTyr: 2.275 ± 0.042	0.001AlaXaa: 0.001 ± 0.001
Cys	0.9CysAla: 0.9 ± 0.027	0.086CysCys: 0.086 ± 0.008	0.482CysAsp: 0.482 ± 0.017	0.394CysGlu: 0.394 ± 0.017	0.216CysPhe: 0.216 ± 0.012	0.837CysGly: 0.837 ± 0.026	0.181CysHis: 0.181 ± 0.012	0.218CysIle: 0.218 ± 0.012	0.11CysLys: 0.11 ± 0.009	0.665CysLeu: 0.665 ± 0.021	0.097CysMet: 0.097 ± 0.008	0.15CysAsn: 0.15 ± 0.011	0.432CysPro: 0.432 ± 0.019	0.171CysGln: 0.171 ± 0.011	0.522CysArg: 0.522 ± 0.02	0.473CysSer: 0.473 ± 0.018	0.462CysThr: 0.462 ± 0.018	0.577CysVal: 0.577 ± 0.02	0.114CysTrp: 0.114 ± 0.008	0.146CysTyr: 0.146 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.068AspAla: 8.068 ± 0.076	0.383AspCys: 0.383 ± 0.016	4.57AspAsp: 4.57 ± 0.059	4.401AspGlu: 4.401 ± 0.053	1.744AspPhe: 1.744 ± 0.035	6.638AspGly: 6.638 ± 0.084	1.566AspHis: 1.566 ± 0.036	2.19AspIle: 2.19 ± 0.045	1.263AspLys: 1.263 ± 0.035	7.731AspLeu: 7.731 ± 0.082	0.827AspMet: 0.827 ± 0.021	1.074AspAsn: 1.074 ± 0.03	4.553AspPro: 4.553 ± 0.058	1.912AspGln: 1.912 ± 0.037	4.818AspArg: 4.818 ± 0.065	2.557AspSer: 2.557 ± 0.05	2.982AspThr: 2.982 ± 0.047	5.991AspVal: 5.991 ± 0.067	0.976AspTrp: 0.976 ± 0.024	1.177AspTyr: 1.177 ± 0.027	0.001AspXaa: 0.001 ± 0.001
Glu	6.981GluAla: 6.981 ± 0.086	0.354GluCys: 0.354 ± 0.017	3.377GluAsp: 3.377 ± 0.052	3.439GluGlu: 3.439 ± 0.051	1.593GluPhe: 1.593 ± 0.028	4.423GluGly: 4.423 ± 0.062	1.558GluHis: 1.558 ± 0.031	2.851GluIle: 2.851 ± 0.044	1.422GluLys: 1.422 ± 0.034	6.572GluLeu: 6.572 ± 0.081	1.018GluMet: 1.018 ± 0.031	1.053GluAsn: 1.053 ± 0.028	3.186GluPro: 3.186 ± 0.057	2.373GluGln: 2.373 ± 0.05	5.03GluArg: 5.03 ± 0.065	2.805GluSer: 2.805 ± 0.047	2.906GluThr: 2.906 ± 0.046	5.73GluVal: 5.73 ± 0.063	0.82GluTrp: 0.82 ± 0.023	1.037GluTyr: 1.037 ± 0.026	0.001GluXaa: 0.001 ± 0.001
Phe	3.786PheAla: 3.786 ± 0.051	0.297PheCys: 0.297 ± 0.015	2.16PheAsp: 2.16 ± 0.042	1.691PheGlu: 1.691 ± 0.032	0.865PhePhe: 0.865 ± 0.025	3.095PheGly: 3.095 ± 0.048	0.607PheHis: 0.607 ± 0.019	0.866PheIle: 0.866 ± 0.025	0.523PheLys: 0.523 ± 0.019	2.633PheLeu: 2.633 ± 0.046	0.418PheMet: 0.418 ± 0.016	0.665PheAsn: 0.665 ± 0.022	1.295PhePro: 1.295 ± 0.03	0.678PheGln: 0.678 ± 0.021	1.788PheArg: 1.788 ± 0.033	1.53PheSer: 1.53 ± 0.032	1.943PheThr: 1.943 ± 0.037	2.577PheVal: 2.577 ± 0.045	0.434PheTrp: 0.434 ± 0.019	0.583PheTyr: 0.583 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	10.651GlyAla: 10.651 ± 0.104	0.762GlyCys: 0.762 ± 0.025	5.476GlyAsp: 5.476 ± 0.07	5.305GlyGlu: 5.305 ± 0.058	3.011GlyPhe: 3.011 ± 0.046	8.308GlyGly: 8.308 ± 0.098	2.049GlyHis: 2.049 ± 0.039	4.013GlyIle: 4.013 ± 0.064	2.284GlyLys: 2.284 ± 0.045	9.387GlyLeu: 9.387 ± 0.093	1.888GlyMet: 1.888 ± 0.039	1.88GlyAsn: 1.88 ± 0.04	4.458GlyPro: 4.458 ± 0.06	2.692GlyGln: 2.692 ± 0.043	6.768GlyArg: 6.768 ± 0.07	5.464GlySer: 5.464 ± 0.077	5.969GlyThr: 5.969 ± 0.077	7.993GlyVal: 7.993 ± 0.086	1.688GlyTrp: 1.688 ± 0.033	2.077GlyTyr: 2.077 ± 0.038	0.001GlyXaa: 0.001 ± 0.001
His	2.434HisAla: 2.434 ± 0.051	0.201HisCys: 0.201 ± 0.013	1.488HisAsp: 1.488 ± 0.035	1.193HisGlu: 1.193 ± 0.029	0.645HisPhe: 0.645 ± 0.02	2.137HisGly: 2.137 ± 0.04	0.727HisHis: 0.727 ± 0.024	0.575HisIle: 0.575 ± 0.02	0.321HisLys: 0.321 ± 0.015	2.438HisLeu: 2.438 ± 0.045	0.298HisMet: 0.298 ± 0.014	0.346HisAsn: 0.346 ± 0.015	1.5HisPro: 1.5 ± 0.035	0.637HisGln: 0.637 ± 0.02	1.817HisArg: 1.817 ± 0.04	0.859HisSer: 0.859 ± 0.024	1.075HisThr: 1.075 ± 0.026	1.907HisVal: 1.907 ± 0.041	0.288HisTrp: 0.288 ± 0.014	0.445HisTyr: 0.445 ± 0.017	0.001HisXaa: 0.001 ± 0.001
Ile	5.795IleAla: 5.795 ± 0.067	0.306IleCys: 0.306 ± 0.014	3.049IleAsp: 3.049 ± 0.045	2.735IleGlu: 2.735 ± 0.048	0.894IlePhe: 0.894 ± 0.029	4.228IleGly: 4.228 ± 0.057	0.715IleHis: 0.715 ± 0.02	1.186IleIle: 1.186 ± 0.035	0.876IleLys: 0.876 ± 0.027	2.784IleLeu: 2.784 ± 0.05	0.49IleMet: 0.49 ± 0.019	0.932IleAsn: 0.932 ± 0.024	1.929IlePro: 1.929 ± 0.041	0.862IleGln: 0.862 ± 0.025	2.452IleArg: 2.452 ± 0.043	2.088IleSer: 2.088 ± 0.038	2.613IleThr: 2.613 ± 0.05	3.461IleVal: 3.461 ± 0.047	0.432IleTrp: 0.432 ± 0.016	0.627IleTyr: 0.627 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.787LysAla: 2.787 ± 0.059	0.102LysCys: 0.102 ± 0.01	1.278LysAsp: 1.278 ± 0.032	1.1LysGlu: 1.1 ± 0.032	0.518LysPhe: 0.518 ± 0.018	1.718LysGly: 1.718 ± 0.042	0.424LysHis: 0.424 ± 0.015	0.927LysIle: 0.927 ± 0.026	0.75LysLys: 0.75 ± 0.036	1.848LysLeu: 1.848 ± 0.04	0.395LysMet: 0.395 ± 0.02	0.483LysAsn: 0.483 ± 0.021	1.178LysPro: 1.178 ± 0.029	0.683LysGln: 0.683 ± 0.024	1.39LysArg: 1.39 ± 0.033	1.102LysSer: 1.102 ± 0.025	1.142LysThr: 1.142 ± 0.031	2.209LysVal: 2.209 ± 0.049	0.258LysTrp: 0.258 ± 0.013	0.449LysTyr: 0.449 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.961LeuAla: 14.961 ± 0.112	0.719LeuCys: 0.719 ± 0.024	7.202LeuAsp: 7.202 ± 0.068	5.506LeuGlu: 5.506 ± 0.07	2.442LeuPhe: 2.442 ± 0.042	9.594LeuGly: 9.594 ± 0.099	2.003LeuHis: 2.003 ± 0.04	3.249LeuIle: 3.249 ± 0.052	1.808LeuLys: 1.808 ± 0.039	10.669LeuLeu: 10.669 ± 0.123	1.611LeuMet: 1.611 ± 0.032	1.657LeuAsn: 1.657 ± 0.034	5.727LeuPro: 5.727 ± 0.069	2.456LeuGln: 2.456 ± 0.044	7.571LeuArg: 7.571 ± 0.083	5.409LeuSer: 5.409 ± 0.063	6.528LeuThr: 6.528 ± 0.075	10.411LeuVal: 10.411 ± 0.101	1.18LeuTrp: 1.18 ± 0.032	1.463LeuTyr: 1.463 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	2.325MetAla: 2.325 ± 0.048	0.133MetCys: 0.133 ± 0.01	0.852MetAsp: 0.852 ± 0.023	0.756MetGlu: 0.756 ± 0.021	0.5MetPhe: 0.5 ± 0.017	1.365MetGly: 1.365 ± 0.029	0.341MetHis: 0.341 ± 0.017	0.735MetIle: 0.735 ± 0.024	0.459MetLys: 0.459 ± 0.016	1.79MetLeu: 1.79 ± 0.038	0.346MetMet: 0.346 ± 0.015	0.442MetAsn: 0.442 ± 0.016	1.013MetPro: 1.013 ± 0.024	0.446MetGln: 0.446 ± 0.017	1.359MetArg: 1.359 ± 0.033	1.467MetSer: 1.467 ± 0.032	1.639MetThr: 1.639 ± 0.032	1.512MetVal: 1.512 ± 0.029	0.201MetTrp: 0.201 ± 0.011	0.275MetTyr: 0.275 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.323AsnAla: 2.323 ± 0.044	0.164AsnCys: 0.164 ± 0.011	1.218AsnAsp: 1.218 ± 0.035	0.937AsnGlu: 0.937 ± 0.026	0.546AsnPhe: 0.546 ± 0.021	1.886AsnGly: 1.886 ± 0.041	0.402AsnHis: 0.402 ± 0.016	0.752AsnIle: 0.752 ± 0.027	0.44AsnLys: 0.44 ± 0.018	1.879AsnLeu: 1.879 ± 0.034	0.299AsnMet: 0.299 ± 0.015	0.458AsnAsn: 0.458 ± 0.019	1.432AsnPro: 1.432 ± 0.035	0.607AsnGln: 0.607 ± 0.022	1.242AsnArg: 1.242 ± 0.03	0.892AsnSer: 0.892 ± 0.029	1.05AsnThr: 1.05 ± 0.029	1.544AsnVal: 1.544 ± 0.033	0.287AsnTrp: 0.287 ± 0.012	0.43AsnTyr: 0.43 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	7.137ProAla: 7.137 ± 0.083	0.288ProCys: 0.288 ± 0.016	4.349ProAsp: 4.349 ± 0.059	3.92ProGlu: 3.92 ± 0.057	1.491ProPhe: 1.491 ± 0.033	5.506ProGly: 5.506 ± 0.059	1.112ProHis: 1.112 ± 0.03	1.842ProIle: 1.842 ± 0.037	1.057ProLys: 1.057 ± 0.028	4.625ProLeu: 4.625 ± 0.056	0.985ProMet: 0.985 ± 0.025	0.902ProAsn: 0.902 ± 0.024	2.644ProPro: 2.644 ± 0.056	1.356ProGln: 1.356 ± 0.032	3.358ProArg: 3.358 ± 0.053	3.138ProSer: 3.138 ± 0.053	3.562ProThr: 3.562 ± 0.047	5.341ProVal: 5.341 ± 0.064	0.886ProTrp: 0.886 ± 0.027	1.145ProTyr: 1.145 ± 0.031	0.001ProXaa: 0.001 ± 0.001
Gln	3.411GlnAla: 3.411 ± 0.06	0.148GlnCys: 0.148 ± 0.01	1.262GlnAsp: 1.262 ± 0.027	1.385GlnGlu: 1.385 ± 0.033	0.804GlnPhe: 0.804 ± 0.024	2.168GlnGly: 2.168 ± 0.036	0.671GlnHis: 0.671 ± 0.022	1.241GlnIle: 1.241 ± 0.031	0.584GlnLys: 0.584 ± 0.021	3.102GlnLeu: 3.102 ± 0.05	0.57GlnMet: 0.57 ± 0.019	0.481GlnAsn: 0.481 ± 0.019	1.643GlnPro: 1.643 ± 0.043	1.159GlnGln: 1.159 ± 0.033	2.333GlnArg: 2.333 ± 0.041	1.269GlnSer: 1.269 ± 0.033	1.434GlnThr: 1.434 ± 0.03	2.937GlnVal: 2.937 ± 0.042	0.478GlnTrp: 0.478 ± 0.016	0.524GlnTyr: 0.524 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	8.592ArgAla: 8.592 ± 0.103	0.455ArgCys: 0.455 ± 0.019	4.353ArgAsp: 4.353 ± 0.063	4.293ArgGlu: 4.293 ± 0.068	2.291ArgPhe: 2.291 ± 0.034	5.361ArgGly: 5.361 ± 0.062	1.737ArgHis: 1.737 ± 0.035	3.486ArgIle: 3.486 ± 0.051	1.54ArgLys: 1.54 ± 0.036	7.707ArgLeu: 7.707 ± 0.087	1.709ArgMet: 1.709 ± 0.032	1.375ArgAsn: 1.375 ± 0.029	3.86ArgPro: 3.86 ± 0.055	2.079ArgGln: 2.079 ± 0.044	6.617ArgArg: 6.617 ± 0.093	4.266ArgSer: 4.266 ± 0.052	4.77ArgThr: 4.77 ± 0.061	5.834ArgVal: 5.834 ± 0.079	1.323ArgTrp: 1.323 ± 0.032	1.528ArgTyr: 1.528 ± 0.032	0.001ArgXaa: 0.001 ± 0.001
Ser	6.687SerAla: 6.687 ± 0.079	0.403SerCys: 0.403 ± 0.019	3.177SerAsp: 3.177 ± 0.055	2.716SerGlu: 2.716 ± 0.044	1.75SerPhe: 1.75 ± 0.034	5.916SerGly: 5.916 ± 0.075	0.995SerHis: 0.995 ± 0.028	2.108SerIle: 2.108 ± 0.038	1.039SerLys: 1.039 ± 0.029	5.236SerLeu: 5.236 ± 0.056	1.241SerMet: 1.241 ± 0.032	1.021SerAsn: 1.021 ± 0.024	2.973SerPro: 2.973 ± 0.046	1.316SerGln: 1.316 ± 0.033	3.69SerArg: 3.69 ± 0.05	3.342SerSer: 3.342 ± 0.058	3.536SerThr: 3.536 ± 0.059	4.641SerVal: 4.641 ± 0.053	0.929SerTrp: 0.929 ± 0.023	1.446SerTyr: 1.446 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	7.679ThrAla: 7.679 ± 0.076	0.475ThrCys: 0.475 ± 0.019	3.776ThrAsp: 3.776 ± 0.057	3.16ThrGlu: 3.16 ± 0.049	1.902ThrPhe: 1.902 ± 0.04	6.272ThrGly: 6.272 ± 0.078	1.168ThrHis: 1.168 ± 0.027	2.457ThrIle: 2.457 ± 0.041	1.222ThrLys: 1.222 ± 0.031	5.827ThrLeu: 5.827 ± 0.059	1.045ThrMet: 1.045 ± 0.023	1.202ThrAsn: 1.202 ± 0.029	3.874ThrPro: 3.874 ± 0.053	1.415ThrGln: 1.415 ± 0.037	3.891ThrArg: 3.891 ± 0.049	3.902ThrSer: 3.902 ± 0.063	4.324ThrThr: 4.324 ± 0.06	5.782ThrVal: 5.782 ± 0.068	1.063ThrTrp: 1.063 ± 0.026	1.428ThrTyr: 1.428 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	12.415ValAla: 12.415 ± 0.105	0.675ValCys: 0.675 ± 0.021	6.209ValAsp: 6.209 ± 0.071	5.711ValGlu: 5.711 ± 0.067	2.409ValPhe: 2.409 ± 0.045	7.634ValGly: 7.634 ± 0.073	1.896ValHis: 1.896 ± 0.037	3.663ValIle: 3.663 ± 0.057	1.691ValLys: 1.691 ± 0.04	10.185ValLeu: 10.185 ± 0.108	1.546ValMet: 1.546 ± 0.038	1.798ValAsn: 1.798 ± 0.037	5.041ValPro: 5.041 ± 0.056	2.254ValGln: 2.254 ± 0.043	6.869ValArg: 6.869 ± 0.07	4.721ValSer: 4.721 ± 0.058	5.923ValThr: 5.923 ± 0.08	10.041ValVal: 10.041 ± 0.113	1.135ValTrp: 1.135 ± 0.029	1.365ValTyr: 1.365 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.605TrpAla: 1.605 ± 0.036	0.15TrpCys: 0.15 ± 0.01	0.826TrpAsp: 0.826 ± 0.026	0.738TrpGlu: 0.738 ± 0.023	0.558TrpPhe: 0.558 ± 0.018	1.041TrpGly: 1.041 ± 0.027	0.333TrpHis: 0.333 ± 0.015	0.65TrpIle: 0.65 ± 0.021	0.35TrpLys: 0.35 ± 0.016	1.745TrpLeu: 1.745 ± 0.04	0.291TrpMet: 0.291 ± 0.014	0.381TrpAsn: 0.381 ± 0.014	0.757TrpPro: 0.757 ± 0.021	0.522TrpGln: 0.522 ± 0.019	1.212TrpArg: 1.212 ± 0.031	1.061TrpSer: 1.061 ± 0.033	1.033TrpThr: 1.033 ± 0.028	1.214TrpVal: 1.214 ± 0.027	0.363TrpTrp: 0.363 ± 0.016	0.285TrpTyr: 0.285 ± 0.015	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.389TyrAla: 2.389 ± 0.042	0.17TyrCys: 0.17 ± 0.011	1.759TyrAsp: 1.759 ± 0.038	1.005TyrGlu: 1.005 ± 0.025	0.693TyrPhe: 0.693 ± 0.023	1.899TyrGly: 1.899 ± 0.038	0.312TyrHis: 0.312 ± 0.016	0.489TyrIle: 0.489 ± 0.022	0.36TyrLys: 0.36 ± 0.016	2.054TyrLeu: 2.054 ± 0.038	0.194TyrMet: 0.194 ± 0.012	0.348TyrAsn: 0.348 ± 0.019	0.918TyrPro: 0.918 ± 0.024	0.517TyrGln: 0.517 ± 0.019	1.456TyrArg: 1.456 ± 0.031	1.005TyrSer: 1.005 ± 0.026	1.027TyrThr: 1.027 ± 0.026	1.807TyrVal: 1.807 ± 0.036	0.303TyrTrp: 0.303 ± 0.014	0.418TyrTyr: 0.418 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4603 proteins (1497011 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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