Streptomyces sp. MnatMP-M17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C5FJ43|A0A1C5FJ43_9ACTN Cobyric acid synthase OS=Streptomyces sp. MnatMP-M17 OX=1839780 GN=cobQ PE=3 SV=1
MM1 pKa = 7.62PVPTTGHH8 pKa = 4.65TTEE11 pKa = 5.91SLTTWLAEE19 pKa = 4.7RR20 pKa = 11.84IALYY24 pKa = 10.37LKK26 pKa = 10.02RR27 pKa = 11.84QPAEE31 pKa = 3.77IDD33 pKa = 3.19PDD35 pKa = 3.93VPLAEE40 pKa = 4.49YY41 pKa = 11.0GLDD44 pKa = 3.42SVAALSLCGDD54 pKa = 3.54IEE56 pKa = 4.78DD57 pKa = 5.69DD58 pKa = 3.72YY59 pKa = 11.64DD60 pKa = 4.52LVMEE64 pKa = 4.67PTVAWDD70 pKa = 3.58YY71 pKa = 8.54PTVHH75 pKa = 7.02ALAGHH80 pKa = 6.33LVEE83 pKa = 5.68EE84 pKa = 4.78LAADD88 pKa = 4.24TAQGGGAA95 pKa = 3.77

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C5D172|A0A1C5D172_9ACTN Helix-turn-helix domain-containing protein OS=Streptomyces sp. MnatMP-M17 OX=1839780 GN=GA0115259_102276 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.44GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7977

0

7977

2364209

24

3791

296.4

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.235 ± 0.043

0.781 ± 0.008

5.935 ± 0.023

5.773 ± 0.032

2.691 ± 0.016

9.5 ± 0.029

2.245 ± 0.013

3.348 ± 0.016

2.179 ± 0.025

10.159 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.73 ± 0.012

1.869 ± 0.019

6.104 ± 0.022

2.811 ± 0.017

8.038 ± 0.033

5.4 ± 0.022

6.363 ± 0.032

8.194 ± 0.026

1.508 ± 0.013

2.139 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski