Amino acid dipepetide frequency for Streptomyces sp. MnatMP-M17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.841AlaAla: 19.841 ± 0.124
1.005AlaCys: 1.005 ± 0.021
8.103AlaAsp: 8.103 ± 0.067
8.9AlaGlu: 8.9 ± 0.066
3.42AlaPhe: 3.42 ± 0.038
12.907AlaGly: 12.907 ± 0.084
2.723AlaHis: 2.723 ± 0.04
3.541AlaIle: 3.541 ± 0.048
2.88AlaLys: 2.88 ± 0.046
14.069AlaLeu: 14.069 ± 0.099
2.512AlaMet: 2.512 ± 0.038
1.979AlaAsn: 1.979 ± 0.032
6.842AlaPro: 6.842 ± 0.072
3.75AlaGln: 3.75 ± 0.039
10.043AlaArg: 10.043 ± 0.079
6.211AlaSer: 6.211 ± 0.053
7.079AlaThr: 7.079 ± 0.064
12.066AlaVal: 12.066 ± 0.09
1.768AlaTrp: 1.768 ± 0.029
2.715AlaTyr: 2.715 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.068CysAla: 1.068 ± 0.023
0.091CysCys: 0.091 ± 0.008
0.463CysAsp: 0.463 ± 0.014
0.392CysGlu: 0.392 ± 0.012
0.209CysPhe: 0.209 ± 0.009
0.932CysGly: 0.932 ± 0.021
0.195CysHis: 0.195 ± 0.009
0.16CysIle: 0.16 ± 0.009
0.117CysLys: 0.117 ± 0.008
0.731CysLeu: 0.731 ± 0.02
0.109CysMet: 0.109 ± 0.007
0.137CysAsn: 0.137 ± 0.007
0.506CysPro: 0.506 ± 0.017
0.168CysGln: 0.168 ± 0.008
0.638CysArg: 0.638 ± 0.016
0.455CysSer: 0.455 ± 0.014
0.507CysThr: 0.507 ± 0.015
0.641CysVal: 0.641 ± 0.018
0.127CysTrp: 0.127 ± 0.007
0.16CysTyr: 0.16 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.441AspAla: 7.441 ± 0.055
0.415AspCys: 0.415 ± 0.016
3.402AspAsp: 3.402 ± 0.042
3.874AspGlu: 3.874 ± 0.049
1.653AspPhe: 1.653 ± 0.028
6.626AspGly: 6.626 ± 0.056
1.371AspHis: 1.371 ± 0.026
2.103AspIle: 2.103 ± 0.03
1.183AspLys: 1.183 ± 0.027
5.924AspLeu: 5.924 ± 0.055
0.824AspMet: 0.824 ± 0.016
1.065AspAsn: 1.065 ± 0.023
4.361AspPro: 4.361 ± 0.047
1.62AspGln: 1.62 ± 0.026
4.875AspArg: 4.875 ± 0.049
2.811AspSer: 2.811 ± 0.037
3.395AspThr: 3.395 ± 0.044
4.304AspVal: 4.304 ± 0.047
1.006AspTrp: 1.006 ± 0.021
1.095AspTyr: 1.095 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.232GluAla: 7.232 ± 0.063
0.395GluCys: 0.395 ± 0.013
2.836GluAsp: 2.836 ± 0.042
3.572GluGlu: 3.572 ± 0.043
1.474GluPhe: 1.474 ± 0.026
4.214GluGly: 4.214 ± 0.046
1.485GluHis: 1.485 ± 0.025
2.382GluIle: 2.382 ± 0.031
1.438GluLys: 1.438 ± 0.027
6.932GluLeu: 6.932 ± 0.06
0.894GluMet: 0.894 ± 0.021
1.105GluAsn: 1.105 ± 0.022
3.605GluPro: 3.605 ± 0.041
2.257GluGln: 2.257 ± 0.035
5.586GluArg: 5.586 ± 0.049
2.913GluSer: 2.913 ± 0.042
3.086GluThr: 3.086 ± 0.036
4.288GluVal: 4.288 ± 0.049
0.831GluTrp: 0.831 ± 0.021
1.201GluTyr: 1.201 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.53PheAla: 3.53 ± 0.036
0.264PheCys: 0.264 ± 0.013
1.937PheAsp: 1.937 ± 0.034
1.425PheGlu: 1.425 ± 0.025
0.843PhePhe: 0.843 ± 0.023
2.883PheGly: 2.883 ± 0.042
0.606PheHis: 0.606 ± 0.016
0.815PheIle: 0.815 ± 0.019
0.525PheLys: 0.525 ± 0.015
2.507PheLeu: 2.507 ± 0.035
0.392PheMet: 0.392 ± 0.014
0.588PheAsn: 0.588 ± 0.015
1.378PhePro: 1.378 ± 0.021
0.701PheGln: 0.701 ± 0.018
1.843PheArg: 1.843 ± 0.029
1.565PheSer: 1.565 ± 0.027
2.074PheThr: 2.074 ± 0.028
2.067PheVal: 2.067 ± 0.031
0.398PheTrp: 0.398 ± 0.014
0.576PheTyr: 0.576 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
10.968GlyAla: 10.968 ± 0.078
0.83GlyCys: 0.83 ± 0.019
4.987GlyAsp: 4.987 ± 0.053
5.174GlyGlu: 5.174 ± 0.047
2.81GlyPhe: 2.81 ± 0.038
8.989GlyGly: 8.989 ± 0.1
2.361GlyHis: 2.361 ± 0.031
3.613GlyIle: 3.613 ± 0.046
2.433GlyLys: 2.433 ± 0.043
9.323GlyLeu: 9.323 ± 0.068
1.953GlyMet: 1.953 ± 0.031
1.844GlyAsn: 1.844 ± 0.039
5.442GlyPro: 5.442 ± 0.049
2.777GlyGln: 2.777 ± 0.04
7.917GlyArg: 7.917 ± 0.067
5.738GlySer: 5.738 ± 0.063
6.796GlyThr: 6.796 ± 0.06
7.097GlyVal: 7.097 ± 0.07
1.689GlyTrp: 1.689 ± 0.029
2.26GlyTyr: 2.26 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.522HisAla: 2.522 ± 0.037
0.201HisCys: 0.201 ± 0.009
1.321HisAsp: 1.321 ± 0.025
1.227HisGlu: 1.227 ± 0.025
0.653HisPhe: 0.653 ± 0.016
2.383HisGly: 2.383 ± 0.032
0.692HisHis: 0.692 ± 0.018
0.734HisIle: 0.734 ± 0.02
0.353HisLys: 0.353 ± 0.012
2.249HisLeu: 2.249 ± 0.032
0.33HisMet: 0.33 ± 0.012
0.394HisAsn: 0.394 ± 0.015
1.72HisPro: 1.72 ± 0.031
0.689HisGln: 0.689 ± 0.016
2.063HisArg: 2.063 ± 0.032
1.085HisSer: 1.085 ± 0.021
1.398HisThr: 1.398 ± 0.023
1.589HisVal: 1.589 ± 0.026
0.363HisTrp: 0.363 ± 0.014
0.481HisTyr: 0.481 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
4.846IleAla: 4.846 ± 0.057
0.317IleCys: 0.317 ± 0.013
2.37IleAsp: 2.37 ± 0.036
2.143IleGlu: 2.143 ± 0.035
0.738IlePhe: 0.738 ± 0.018
3.649IleGly: 3.649 ± 0.047
0.655IleHis: 0.655 ± 0.015
0.956IleIle: 0.956 ± 0.024
0.792IleLys: 0.792 ± 0.019
2.495IleLeu: 2.495 ± 0.039
0.447IleMet: 0.447 ± 0.016
0.766IleAsn: 0.766 ± 0.018
1.986IlePro: 1.986 ± 0.031
0.799IleGln: 0.799 ± 0.02
2.422IleArg: 2.422 ± 0.032
1.896IleSer: 1.896 ± 0.029
2.479IleThr: 2.479 ± 0.035
2.714IleVal: 2.714 ± 0.035
0.438IleTrp: 0.438 ± 0.015
0.575IleTyr: 0.575 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.87LysAla: 2.87 ± 0.047
0.127LysCys: 0.127 ± 0.008
1.378LysAsp: 1.378 ± 0.027
1.264LysGlu: 1.264 ± 0.026
0.445LysPhe: 0.445 ± 0.015
1.871LysGly: 1.871 ± 0.035
0.425LysHis: 0.425 ± 0.015
0.904LysIle: 0.904 ± 0.021
0.833LysLys: 0.833 ± 0.024
2.062LysLeu: 2.062 ± 0.034
0.376LysMet: 0.376 ± 0.014
0.582LysAsn: 0.582 ± 0.019
1.351LysPro: 1.351 ± 0.029
0.706LysGln: 0.706 ± 0.02
1.474LysArg: 1.474 ± 0.027
1.206LysSer: 1.206 ± 0.027
1.35LysThr: 1.35 ± 0.024
1.831LysVal: 1.831 ± 0.033
0.27LysTrp: 0.27 ± 0.01
0.461LysTyr: 0.461 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.557LeuAla: 14.557 ± 0.096
0.821LeuCys: 0.821 ± 0.019
6.65LeuAsp: 6.65 ± 0.063
4.628LeuGlu: 4.628 ± 0.049
2.568LeuPhe: 2.568 ± 0.038
9.002LeuGly: 9.002 ± 0.079
2.211LeuHis: 2.211 ± 0.029
3.49LeuIle: 3.49 ± 0.044
2.014LeuLys: 2.014 ± 0.034
11.03LeuLeu: 11.03 ± 0.101
1.647LeuMet: 1.647 ± 0.025
1.782LeuAsn: 1.782 ± 0.03
6.448LeuPro: 6.448 ± 0.058
2.168LeuGln: 2.168 ± 0.031
8.608LeuArg: 8.608 ± 0.071
5.541LeuSer: 5.541 ± 0.047
6.946LeuThr: 6.946 ± 0.064
8.307LeuVal: 8.307 ± 0.071
1.259LeuTrp: 1.259 ± 0.025
1.912LeuTyr: 1.912 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.282MetAla: 2.282 ± 0.034
0.136MetCys: 0.136 ± 0.008
0.903MetAsp: 0.903 ± 0.024
0.828MetGlu: 0.828 ± 0.022
0.416MetPhe: 0.416 ± 0.013
1.307MetGly: 1.307 ± 0.028
0.339MetHis: 0.339 ± 0.01
0.633MetIle: 0.633 ± 0.017
0.433MetLys: 0.433 ± 0.013
1.623MetLeu: 1.623 ± 0.024
0.29MetMet: 0.29 ± 0.011
0.442MetAsn: 0.442 ± 0.014
1.145MetPro: 1.145 ± 0.022
0.399MetGln: 0.399 ± 0.013
1.408MetArg: 1.408 ± 0.026
1.36MetSer: 1.36 ± 0.022
1.559MetThr: 1.559 ± 0.028
1.263MetVal: 1.263 ± 0.025
0.198MetTrp: 0.198 ± 0.008
0.331MetTyr: 0.331 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.317AsnAla: 2.317 ± 0.031
0.185AsnCys: 0.185 ± 0.009
1.006AsnAsp: 1.006 ± 0.02
0.864AsnGlu: 0.864 ± 0.018
0.491AsnPhe: 0.491 ± 0.014
2.148AsnGly: 2.148 ± 0.039
0.413AsnHis: 0.413 ± 0.012
0.677AsnIle: 0.677 ± 0.018
0.446AsnLys: 0.446 ± 0.015
1.623AsnLeu: 1.623 ± 0.031
0.301AsnMet: 0.301 ± 0.012
0.481AsnAsn: 0.481 ± 0.016
1.387AsnPro: 1.387 ± 0.021
0.559AsnGln: 0.559 ± 0.019
1.37AsnArg: 1.37 ± 0.026
1.063AsnSer: 1.063 ± 0.023
1.227AsnThr: 1.227 ± 0.029
1.376AsnVal: 1.376 ± 0.029
0.313AsnTrp: 0.313 ± 0.011
0.445AsnTyr: 0.445 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
8.506ProAla: 8.506 ± 0.07
0.33ProCys: 0.33 ± 0.013
4.461ProAsp: 4.461 ± 0.048
4.431ProGlu: 4.431 ± 0.049
1.501ProPhe: 1.501 ± 0.024
6.689ProGly: 6.689 ± 0.056
1.328ProHis: 1.328 ± 0.026
1.3ProIle: 1.3 ± 0.023
1.185ProLys: 1.185 ± 0.026
5.366ProLeu: 5.366 ± 0.044
1.032ProMet: 1.032 ± 0.019
0.981ProAsn: 0.981 ± 0.02
3.54ProPro: 3.54 ± 0.056
1.743ProGln: 1.743 ± 0.031
3.911ProArg: 3.911 ± 0.046
3.429ProSer: 3.429 ± 0.042
3.251ProThr: 3.251 ± 0.039
5.687ProVal: 5.687 ± 0.053
0.89ProTrp: 0.89 ± 0.019
1.477ProTyr: 1.477 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.573GlnAla: 3.573 ± 0.041
0.183GlnCys: 0.183 ± 0.009
1.456GlnAsp: 1.456 ± 0.026
1.491GlnGlu: 1.491 ± 0.027
0.703GlnPhe: 0.703 ± 0.019
2.344GlnGly: 2.344 ± 0.034
0.673GlnHis: 0.673 ± 0.018
1.22GlnIle: 1.22 ± 0.024
0.634GlnLys: 0.634 ± 0.019
3.09GlnLeu: 3.09 ± 0.036
0.497GlnMet: 0.497 ± 0.014
0.554GlnAsn: 0.554 ± 0.016
1.731GlnPro: 1.731 ± 0.037
1.318GlnGln: 1.318 ± 0.03
2.446GlnArg: 2.446 ± 0.033
1.37GlnSer: 1.37 ± 0.025
1.411GlnThr: 1.411 ± 0.024
2.332GlnVal: 2.332 ± 0.034
0.471GlnTrp: 0.471 ± 0.016
0.613GlnTyr: 0.613 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.729ArgAla: 9.729 ± 0.087
0.613ArgCys: 0.613 ± 0.015
4.197ArgAsp: 4.197 ± 0.047
4.745ArgGlu: 4.745 ± 0.048
2.335ArgPhe: 2.335 ± 0.031
5.678ArgGly: 5.678 ± 0.057
2.074ArgHis: 2.074 ± 0.029
3.488ArgIle: 3.488 ± 0.037
1.741ArgLys: 1.741 ± 0.029
8.844ArgLeu: 8.844 ± 0.075
1.698ArgMet: 1.698 ± 0.028
1.454ArgAsn: 1.454 ± 0.026
5.084ArgPro: 5.084 ± 0.056
2.387ArgGln: 2.387 ± 0.033
7.663ArgArg: 7.663 ± 0.077
4.198ArgSer: 4.198 ± 0.045
5.75ArgThr: 5.75 ± 0.047
5.462ArgVal: 5.462 ± 0.052
1.373ArgTrp: 1.373 ± 0.024
1.867ArgTyr: 1.867 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
7.264SerAla: 7.264 ± 0.061
0.426SerCys: 0.426 ± 0.013
2.912SerAsp: 2.912 ± 0.036
2.571SerGlu: 2.571 ± 0.035
1.626SerPhe: 1.626 ± 0.025
6.563SerGly: 6.563 ± 0.063
1.039SerHis: 1.039 ± 0.02
1.525SerIle: 1.525 ± 0.026
1.087SerLys: 1.087 ± 0.026
4.971SerLeu: 4.971 ± 0.05
1.061SerMet: 1.061 ± 0.02
0.961SerAsn: 0.961 ± 0.022
3.282SerPro: 3.282 ± 0.044
1.325SerGln: 1.325 ± 0.026
3.956SerArg: 3.956 ± 0.042
3.222SerSer: 3.222 ± 0.049
3.305SerThr: 3.305 ± 0.041
4.62SerVal: 4.62 ± 0.041
0.95SerTrp: 0.95 ± 0.018
1.333SerTyr: 1.333 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.459ThrAla: 9.459 ± 0.077
0.401ThrCys: 0.401 ± 0.015
3.917ThrAsp: 3.917 ± 0.042
3.54ThrGlu: 3.54 ± 0.042
1.609ThrPhe: 1.609 ± 0.025
7.198ThrGly: 7.198 ± 0.064
1.177ThrHis: 1.177 ± 0.023
1.811ThrIle: 1.811 ± 0.028
1.202ThrLys: 1.202 ± 0.03
5.662ThrLeu: 5.662 ± 0.05
0.964ThrMet: 0.964 ± 0.021
1.064ThrAsn: 1.064 ± 0.025
4.045ThrPro: 4.045 ± 0.049
1.399ThrGln: 1.399 ± 0.028
3.849ThrArg: 3.849 ± 0.044
3.444ThrSer: 3.444 ± 0.044
4.167ThrThr: 4.167 ± 0.063
6.367ThrVal: 6.367 ± 0.055
0.884ThrTrp: 0.884 ± 0.02
1.468ThrTyr: 1.468 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
9.994ValAla: 9.994 ± 0.071
0.728ValCys: 0.728 ± 0.017
4.653ValAsp: 4.653 ± 0.045
4.541ValGlu: 4.541 ± 0.051
2.303ValPhe: 2.303 ± 0.035
6.174ValGly: 6.174 ± 0.054
1.877ValHis: 1.877 ± 0.032
2.975ValIle: 2.975 ± 0.038
1.734ValLys: 1.734 ± 0.034
9.206ValLeu: 9.206 ± 0.083
1.432ValMet: 1.432 ± 0.026
1.634ValAsn: 1.634 ± 0.027
5.308ValPro: 5.308 ± 0.047
2.084ValGln: 2.084 ± 0.028
7.146ValArg: 7.146 ± 0.053
4.487ValSer: 4.487 ± 0.046
5.517ValThr: 5.517 ± 0.058
7.412ValVal: 7.412 ± 0.066
1.15ValTrp: 1.15 ± 0.024
1.585ValTyr: 1.585 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.576TrpAla: 1.576 ± 0.031
0.158TrpCys: 0.158 ± 0.008
0.825TrpAsp: 0.825 ± 0.021
0.764TrpGlu: 0.764 ± 0.019
0.478TrpPhe: 0.478 ± 0.013
1.038TrpGly: 1.038 ± 0.022
0.378TrpHis: 0.378 ± 0.013
0.594TrpIle: 0.594 ± 0.016
0.365TrpLys: 0.365 ± 0.014
1.76TrpLeu: 1.76 ± 0.031
0.277TrpMet: 0.277 ± 0.01
0.434TrpAsn: 0.434 ± 0.014
0.819TrpPro: 0.819 ± 0.019
0.619TrpGln: 0.619 ± 0.017
1.302TrpArg: 1.302 ± 0.024
0.979TrpSer: 0.979 ± 0.021
1.077TrpThr: 1.077 ± 0.021
0.915TrpVal: 0.915 ± 0.021
0.347TrpTrp: 0.347 ± 0.012
0.373TrpTyr: 0.373 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.78TyrAla: 2.78 ± 0.038
0.181TyrCys: 0.181 ± 0.009
1.572TyrAsp: 1.572 ± 0.032
1.353TyrGlu: 1.353 ± 0.026
0.639TyrPhe: 0.639 ± 0.017
2.406TyrGly: 2.406 ± 0.035
0.424TyrHis: 0.424 ± 0.013
0.562TyrIle: 0.562 ± 0.018
0.391TyrLys: 0.391 ± 0.016
2.118TyrLeu: 2.118 ± 0.031
0.259TyrMet: 0.259 ± 0.01
0.446TyrAsn: 0.446 ± 0.016
1.059TyrPro: 1.059 ± 0.022
0.639TyrGln: 0.639 ± 0.018
1.861TyrArg: 1.861 ± 0.03
1.027TyrSer: 1.027 ± 0.021
1.254TyrThr: 1.254 ± 0.025
1.603TyrVal: 1.603 ± 0.026
0.354TyrTrp: 0.354 ± 0.014
0.458TyrTyr: 0.458 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7977 proteins (2364209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski