Protaetiibacter intestinalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Protaetiibacter

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A387B928|A0A387B928_9MICO Uncharacterized protein OS=Protaetiibacter intestinalis OX=2419774 GN=D7I47_11720 PE=4 SV=1
MM1 pKa = 7.33HH2 pKa = 7.23AHH4 pKa = 7.56SIRR7 pKa = 11.84TGLGIGAVTAVALALVGIPSAIAAADD33 pKa = 4.05GPVEE37 pKa = 4.04AGIFVDD43 pKa = 5.99RR44 pKa = 11.84IDD46 pKa = 4.1GLGDD50 pKa = 3.53DD51 pKa = 6.05FINGVDD57 pKa = 3.55VSSILSEE64 pKa = 4.15EE65 pKa = 4.19EE66 pKa = 4.02SGVVYY71 pKa = 10.86YY72 pKa = 9.45DD73 pKa = 3.91TEE75 pKa = 4.06GHH77 pKa = 5.8QADD80 pKa = 4.62LFDD83 pKa = 3.9VLEE86 pKa = 4.86DD87 pKa = 3.53ADD89 pKa = 3.7INYY92 pKa = 9.17VRR94 pKa = 11.84VRR96 pKa = 11.84VWDD99 pKa = 3.94DD100 pKa = 3.55PYY102 pKa = 11.28DD103 pKa = 4.16AEE105 pKa = 4.58GNGYY109 pKa = 10.66GGGTTDD115 pKa = 4.07LARR118 pKa = 11.84AVEE121 pKa = 3.99IGEE124 pKa = 4.14RR125 pKa = 11.84ATAHH129 pKa = 5.76GMRR132 pKa = 11.84VLVDD136 pKa = 4.63FHH138 pKa = 8.88YY139 pKa = 11.36SDD141 pKa = 4.96FWADD145 pKa = 3.24PAKK148 pKa = 10.54QHH150 pKa = 6.15VPKK153 pKa = 10.36AWEE156 pKa = 5.08GYY158 pKa = 5.39TTEE161 pKa = 4.21QLVTALGAYY170 pKa = 8.85TEE172 pKa = 4.49ASLDD176 pKa = 3.51AFADD180 pKa = 3.59AGVDD184 pKa = 3.26VGMVQIGNEE193 pKa = 4.27TNNTIAGVADD203 pKa = 3.82WDD205 pKa = 3.87GKK207 pKa = 11.07AAMFSAGSAAVRR219 pKa = 11.84EE220 pKa = 4.41VFPDD224 pKa = 3.43ALVALHH230 pKa = 6.12FTNPEE235 pKa = 3.73TPNRR239 pKa = 11.84YY240 pKa = 7.64TGYY243 pKa = 10.17AAQLAAHH250 pKa = 6.74DD251 pKa = 3.65VDD253 pKa = 4.06YY254 pKa = 11.5DD255 pKa = 3.9VFASSYY261 pKa = 8.41YY262 pKa = 10.06NFWHH266 pKa = 7.01GSPEE270 pKa = 3.94NLTSVLSTIADD281 pKa = 3.81SYY283 pKa = 10.9DD284 pKa = 3.37KK285 pKa = 10.99KK286 pKa = 11.66VMVAEE291 pKa = 4.32TSYY294 pKa = 11.72VYY296 pKa = 10.66TFDD299 pKa = 6.26DD300 pKa = 4.01GDD302 pKa = 3.34GWQNSVNAGNFSGDD316 pKa = 3.2YY317 pKa = 9.11PATVQGQADD326 pKa = 5.07LIHH329 pKa = 7.17DD330 pKa = 4.06VMAAVAAVGDD340 pKa = 3.81AGLGVFYY347 pKa = 10.3WEE349 pKa = 4.56PAWIPVGTPDD359 pKa = 4.81QLAANQQKK367 pKa = 8.64WEE369 pKa = 4.07QYY371 pKa = 8.49GSGWASSYY379 pKa = 10.97AGSYY383 pKa = 10.89DD384 pKa = 3.57PVDD387 pKa = 3.46AGVYY391 pKa = 9.97YY392 pKa = 10.83GGSSWDD398 pKa = 3.53NQALFDD404 pKa = 4.15FTGHH408 pKa = 6.87PLEE411 pKa = 4.4SLQTFRR417 pKa = 11.84YY418 pKa = 9.22ARR420 pKa = 11.84TGAVTEE426 pKa = 4.38RR427 pKa = 11.84VATSIEE433 pKa = 4.44TVALTVYY440 pKa = 10.22DD441 pKa = 4.43GSPIALPATIEE452 pKa = 3.8VGYY455 pKa = 10.92NDD457 pKa = 4.64GSTEE461 pKa = 3.99HH462 pKa = 7.07PAVAWSDD469 pKa = 3.34AVDD472 pKa = 4.51WIRR475 pKa = 11.84GPGVYY480 pKa = 10.25SIPGTIASGEE490 pKa = 4.36AITATVTVLQANYY503 pKa = 9.66VVNHH507 pKa = 6.2GFEE510 pKa = 4.62DD511 pKa = 4.65ADD513 pKa = 3.5TSMWSLATNPSGAASITTTSNTNEE537 pKa = 3.89GTKK540 pKa = 10.77ALDD543 pKa = 3.66FWAASAFTIQLQQTVTGVPAGEE565 pKa = 4.13YY566 pKa = 9.27TLQATAQGGALGDD579 pKa = 4.05SDD581 pKa = 5.48TLTLWAYY588 pKa = 9.78TDD590 pKa = 4.97DD591 pKa = 5.24DD592 pKa = 5.56SADD595 pKa = 3.1TWMWLYY601 pKa = 10.9GWRR604 pKa = 11.84DD605 pKa = 3.35YY606 pKa = 11.02RR607 pKa = 11.84TFTTPPLTVGDD618 pKa = 4.72DD619 pKa = 3.7GTVTVGAYY627 pKa = 9.63ISATGGAWGTLDD639 pKa = 3.93DD640 pKa = 4.64LRR642 pKa = 11.84LVRR645 pKa = 11.84YY646 pKa = 9.81IPDD649 pKa = 3.62AALDD653 pKa = 3.7TGALEE658 pKa = 4.21EE659 pKa = 4.99LLDD662 pKa = 4.13EE663 pKa = 5.17ADD665 pKa = 3.83GVDD668 pKa = 3.0RR669 pKa = 11.84ALYY672 pKa = 9.93TEE674 pKa = 5.15GSLGGLDD681 pKa = 4.08DD682 pKa = 4.93AVASAQVVLAGSGDD696 pKa = 3.74QDD698 pKa = 4.22DD699 pKa = 4.14VDD701 pKa = 4.22AVVALLRR708 pKa = 11.84AALDD712 pKa = 3.92GLALAPSGEE721 pKa = 4.21PSGEE725 pKa = 3.81PSGEE729 pKa = 3.96PSEE732 pKa = 5.02GPVDD736 pKa = 3.66APVITLGAGTVTPGGTLHH754 pKa = 5.59VTVSGLEE761 pKa = 3.89EE762 pKa = 4.2GSIEE766 pKa = 3.9IGVASTYY773 pKa = 10.36RR774 pKa = 11.84ALATASVVAGTASATVTIPADD795 pKa = 3.65LEE797 pKa = 4.5AGLHH801 pKa = 6.16HH802 pKa = 6.33IQVRR806 pKa = 11.84DD807 pKa = 3.59GSGALLAEE815 pKa = 4.36AEE817 pKa = 4.35LRR819 pKa = 11.84VLALGDD825 pKa = 3.43TGADD829 pKa = 3.19EE830 pKa = 4.24TVIRR834 pKa = 11.84VLAALGAVLVLGGAAAFAWGRR855 pKa = 11.84RR856 pKa = 11.84RR857 pKa = 11.84AAAA860 pKa = 3.8

Molecular weight: 89.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A387BAS7|A0A387BAS7_9MICO 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Protaetiibacter intestinalis OX=2419774 GN=menA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2836

0

2836

943967

26

9467

332.9

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.027 ± 0.078

0.443 ± 0.012

5.983 ± 0.035

5.888 ± 0.05

3.11 ± 0.032

9.184 ± 0.054

1.9 ± 0.023

4.346 ± 0.034

1.776 ± 0.035

10.514 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.557 ± 0.02

1.8 ± 0.024

5.488 ± 0.043

2.601 ± 0.024

7.414 ± 0.072

5.288 ± 0.058

5.944 ± 0.073

9.241 ± 0.045

1.5 ± 0.024

1.997 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski