Weissella cryptocerci
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2588 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6YRS4|A0A4P6YRS4_9LACO GRAM_POS_ANCHORING domain-containing protein OS=Weissella cryptocerci OX=2506420 GN=EQG49_02190 PE=4 SV=1
MM1 pKa = 6.9 STHH4 pKa = 6.74 AVTIVTQSMVGNEE17 pKa = 4.25 PEE19 pKa = 4.17 PFFDD23 pKa = 5.24 VITFLADD30 pKa = 3.46 LDD32 pKa = 3.92 EE33 pKa = 4.68 TQAEE37 pKa = 4.21 DD38 pKa = 3.32 VAYY41 pKa = 10.57 LRR43 pKa = 11.84 EE44 pKa = 4.13 LAEE47 pKa = 4.29 KK48 pKa = 8.58 TQLSPAYY55 pKa = 9.39 EE56 pKa = 3.9 EE57 pKa = 5.68 DD58 pKa = 3.55 GQKK61 pKa = 10.73 VYY63 pKa = 11.3 DD64 pKa = 3.92 VVGKK68 pKa = 10.89 VIDD71 pKa = 3.99 VYY73 pKa = 11.11 EE74 pKa = 5.21 LIDD77 pKa = 4.68 EE78 pKa = 4.41 ITFTGNPEE86 pKa = 3.9 VNSRR90 pKa = 11.84 QILPAKK96 pKa = 9.85 PMNLEE101 pKa = 3.95 QFIEE105 pKa = 4.7 TYY107 pKa = 10.3 YY108 pKa = 11.01 SDD110 pKa = 4.35 YY111 pKa = 11.15 VLEE114 pKa = 4.64 DD115 pKa = 3.58 AEE117 pKa = 4.48 SS118 pKa = 3.11
Molecular weight: 13.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.579
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.63
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.617
EMBOSS 3.643
Sillero 3.872
Patrickios 1.837
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A4P6YSC4|A0A4P6YSC4_9LACO Uncharacterized protein OS=Weissella cryptocerci OX=2506420 GN=EQG49_03150 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 KK3 pKa = 9.64 LLMPILIGLLICSALVAIVVGSTHH27 pKa = 5.91 VRR29 pKa = 11.84 LDD31 pKa = 3.8 DD32 pKa = 5.33 LITGQTMAWQIVGQLRR48 pKa = 11.84 IPRR51 pKa = 11.84 ILTAIIAGVILGVTGLLLQIVTRR74 pKa = 11.84 NSLVDD79 pKa = 3.61 SSILGLMNGTQFLSLLAGITVPNFFGGKK107 pKa = 8.56 VLAGALLGVVVVVMWRR123 pKa = 11.84 FIVWGRR129 pKa = 11.84 LQNYY133 pKa = 9.95 ALILTGIALAMTFAALTEE151 pKa = 4.25 LLDD154 pKa = 3.61 SGFNVNVSNLAVVTWSQVWVLGIILLASLIVLVLLSSHH192 pKa = 6.47 LKK194 pKa = 9.83 FYY196 pKa = 10.96 AVSAVQARR204 pKa = 11.84 QLGEE208 pKa = 4.12 DD209 pKa = 3.99 EE210 pKa = 5.83 NRR212 pKa = 11.84 TTWFILLVVGLLIGGVTSVIGVVYY236 pKa = 8.77 FVGVILPQLAQLMLPRR252 pKa = 11.84 QSRR255 pKa = 11.84 LKK257 pKa = 10.88 LFVPTALLGALLLLNADD274 pKa = 3.77 TLARR278 pKa = 11.84 TILAPRR284 pKa = 11.84 EE285 pKa = 3.95 LSATAILLVICGPVFIGLVLRR306 pKa = 11.84 KK307 pKa = 9.49 VKK309 pKa = 10.28 HH310 pKa = 5.35 AA311 pKa = 4.26
Molecular weight: 33.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.619
IPC_protein 10.409
Toseland 10.643
ProMoST 10.335
Dawson 10.745
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.95
Grimsley 10.789
Solomon 10.862
Lehninger 10.833
Nozaki 10.643
DTASelect 10.452
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.672
Patrickios 10.701
IPC_peptide 10.862
IPC2_peptide 9.502
IPC2.peptide.svr19 8.607
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2588
0
2588
794941
36
2296
307.2
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.868 ± 0.058
0.221 ± 0.01
5.852 ± 0.049
5.52 ± 0.06
4.253 ± 0.039
6.831 ± 0.054
1.923 ± 0.023
6.885 ± 0.049
6.422 ± 0.059
9.255 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.716 ± 0.028
5.165 ± 0.043
3.489 ± 0.031
4.194 ± 0.036
3.704 ± 0.044
5.714 ± 0.04
6.855 ± 0.062
7.52 ± 0.044
1.059 ± 0.021
3.555 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here