Myroides guanonis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2674 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3SHE0|A0A1I3SHE0_9FLAO Uncharacterized protein OS=Myroides guanonis OX=1150112 GN=SAMN04487893_11047 PE=4 SV=1
MM1 pKa = 7.92 ADD3 pKa = 3.08 IKK5 pKa = 10.4 ITIIDD10 pKa = 3.73 RR11 pKa = 11.84 EE12 pKa = 4.53 GEE14 pKa = 4.07 KK15 pKa = 10.5 HH16 pKa = 6.72 IVDD19 pKa = 4.72 APTDD23 pKa = 3.5 MNMNIMEE30 pKa = 4.73 LVRR33 pKa = 11.84 AYY35 pKa = 9.97 EE36 pKa = 4.0 LAPEE40 pKa = 4.26 GTIGICGGMAMCASCQCYY58 pKa = 9.92 VLNAEE63 pKa = 4.47 VVDD66 pKa = 4.63 LPEE69 pKa = 4.25 MNPDD73 pKa = 3.25 EE74 pKa = 5.24 EE75 pKa = 5.16 AMLWEE80 pKa = 4.31 ARR82 pKa = 11.84 NVQDD86 pKa = 5.01 NSRR89 pKa = 11.84 LGCQIHH95 pKa = 7.12 ISEE98 pKa = 4.67 EE99 pKa = 4.1 LDD101 pKa = 3.41 GLEE104 pKa = 4.54 VEE106 pKa = 5.0 LAPAEE111 pKa = 4.12
Molecular weight: 12.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.77
ProMoST 4.024
Dawson 3.884
Bjellqvist 4.101
Wikipedia 3.77
Rodwell 3.77
Grimsley 3.681
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.139
Thurlkill 3.795
EMBOSS 3.783
Sillero 4.05
Patrickios 1.888
IPC_peptide 3.884
IPC2_peptide 4.037
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|A0A1I3M492|A0A1I3M492_9FLAO 30S ribosomal protein S19 OS=Myroides guanonis OX=1150112 GN=rpsS PE=3 SV=1
MM1 pKa = 7.56 SLTKK5 pKa = 9.82 SEE7 pKa = 3.62 RR8 pKa = 11.84 RR9 pKa = 11.84 QRR11 pKa = 11.84 IKK13 pKa = 10.59 FRR15 pKa = 11.84 VRR17 pKa = 11.84 KK18 pKa = 9.14 IVSGTAARR26 pKa = 11.84 PRR28 pKa = 11.84 LSVFRR33 pKa = 11.84 SNKK36 pKa = 9.35 EE37 pKa = 3.2 IYY39 pKa = 9.93 AQIIDD44 pKa = 4.32 DD45 pKa = 4.18 VNGVTLTAASSRR57 pKa = 11.84 EE58 pKa = 3.9 AGITRR63 pKa = 11.84 GTNIEE68 pKa = 3.97 TAASVGKK75 pKa = 10.38 LIAEE79 pKa = 4.54 KK80 pKa = 10.41 ALKK83 pKa = 10.48 AGIEE87 pKa = 4.27 TVSFDD92 pKa = 3.64 RR93 pKa = 11.84 NGYY96 pKa = 9.22 LYY98 pKa = 10.36 HH99 pKa = 6.83 GRR101 pKa = 11.84 VKK103 pKa = 10.71 SLAEE107 pKa = 3.97 GARR110 pKa = 11.84 EE111 pKa = 3.92 AGLKK115 pKa = 9.57 FF116 pKa = 3.59
Molecular weight: 12.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.736
IPC_protein 10.452
Toseland 10.833
ProMoST 10.482
Dawson 10.906
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.184
Grimsley 10.935
Solomon 11.023
Lehninger 10.994
Nozaki 10.789
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 10.921
IPC_peptide 11.038
IPC2_peptide 9.253
IPC2.peptide.svr19 8.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2674
0
2674
919248
39
3566
343.8
38.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.927 ± 0.048
0.794 ± 0.022
5.432 ± 0.034
6.885 ± 0.044
5.273 ± 0.038
6.331 ± 0.048
1.72 ± 0.019
8.034 ± 0.047
7.612 ± 0.036
9.538 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.279 ± 0.027
6.002 ± 0.045
3.226 ± 0.023
3.371 ± 0.029
3.423 ± 0.031
6.807 ± 0.043
5.621 ± 0.047
6.488 ± 0.04
1.033 ± 0.017
4.203 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here