endosymbiont GvMRE of Glomus versiforme
Average proteome isoelectric point is 7.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2357 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397FNK8|A0A397FNK8_9MOLU 30S ribosomal protein S15 OS=endosymbiont GvMRE of Glomus versiforme OX=2039283 GN=GvMRE_IIg473 PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.63 GIIEE6 pKa = 4.26 VTRR9 pKa = 11.84 ITAATFLAAGSVLNVIYY26 pKa = 10.45 GPQTYY31 pKa = 9.8 EE32 pKa = 3.9 SYY34 pKa = 10.9 FEE36 pKa = 4.14 EE37 pKa = 4.46 QVTPEE42 pKa = 4.32 IIICFDD48 pKa = 3.07 IDD50 pKa = 3.8 GVGRR54 pKa = 11.84 IPLEE58 pKa = 3.79 EE59 pKa = 4.06 EE60 pKa = 4.1 QFDD63 pKa = 3.96 EE64 pKa = 4.77
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 3.986
IPC_protein 3.795
Toseland 3.643
ProMoST 3.872
Dawson 3.745
Bjellqvist 4.012
Wikipedia 3.643
Rodwell 3.643
Grimsley 3.579
Solomon 3.719
Lehninger 3.668
Nozaki 3.897
DTASelect 3.948
Thurlkill 3.694
EMBOSS 3.656
Sillero 3.91
Patrickios 1.837
IPC_peptide 3.719
IPC2_peptide 3.884
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A397FVI5|A0A397FVI5_9MOLU Uncharacterized protein OS=endosymbiont GvMRE of Glomus versiforme OX=2039283 GN=GvMRE_I1g370 PE=4 SV=1
MM1 pKa = 7.05 STIKK5 pKa = 10.48 FKK7 pKa = 10.93 SRR9 pKa = 11.84 KK10 pKa = 9.1 SFSKK14 pKa = 10.6 RR15 pKa = 11.84 FAKK18 pKa = 8.94 KK19 pKa = 7.86 TANGKK24 pKa = 9.32 LKK26 pKa = 10.36 RR27 pKa = 11.84 GKK29 pKa = 10.75 SNTSHH34 pKa = 7.22 LFGNKK39 pKa = 7.13 TSRR42 pKa = 11.84 QRR44 pKa = 11.84 KK45 pKa = 7.52 NSRR48 pKa = 11.84 KK49 pKa = 9.23 VAFLSSSDD57 pKa = 3.49 VKK59 pKa = 10.7 RR60 pKa = 11.84 SRR62 pKa = 11.84 KK63 pKa = 8.82 MINHH67 pKa = 6.66 KK68 pKa = 10.74 SII70 pKa = 3.63
Molecular weight: 8.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.36
IPC2_protein 10.818
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.515
Grimsley 12.559
Solomon 12.998
Lehninger 12.896
Nozaki 12.515
DTASelect 12.486
Thurlkill 12.515
EMBOSS 12.998
Sillero 12.515
Patrickios 12.237
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2357
0
2357
596243
29
2665
253.0
29.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.352 ± 0.051
1.305 ± 0.026
4.539 ± 0.042
8.982 ± 0.082
4.557 ± 0.047
4.44 ± 0.054
1.605 ± 0.019
7.886 ± 0.049
11.442 ± 0.077
9.974 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.35 ± 0.017
6.759 ± 0.044
3.134 ± 0.035
5.161 ± 0.081
4.03 ± 0.038
5.877 ± 0.043
4.923 ± 0.042
4.334 ± 0.035
1.717 ± 0.025
3.634 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here