Apis mellifera associated microvirus 31

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTL1|A0A3S8UTL1_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 31 OX=2494760 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.34LFAMLDD8 pKa = 3.32KK9 pKa = 10.92KK10 pKa = 10.68AGHH13 pKa = 6.69FLQPFPEE20 pKa = 4.42TSTIAALRR28 pKa = 11.84GFEE31 pKa = 4.6IAVNDD36 pKa = 4.18AKK38 pKa = 11.16SIFCRR43 pKa = 11.84FPDD46 pKa = 4.29DD47 pKa = 3.7FSLMEE52 pKa = 4.88LADD55 pKa = 4.11FDD57 pKa = 4.51QEE59 pKa = 4.33TGQISPYY66 pKa = 9.54ATPQDD71 pKa = 3.72LGSARR76 pKa = 11.84TVLKK80 pKa = 10.66KK81 pKa = 10.2PAVQDD86 pKa = 3.62TLPGVQQ92 pKa = 3.73

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTQ2|A0A3S8UTQ2_9VIRU Internal scaffolding protein OS=Apis mellifera associated microvirus 31 OX=2494760 PE=4 SV=1
MM1 pKa = 7.79PCYY4 pKa = 9.96KK5 pKa = 10.4PKK7 pKa = 10.87DD8 pKa = 3.91SVILPGLSSTGKK20 pKa = 10.12AVVLFRR26 pKa = 11.84AGLRR30 pKa = 11.84PLSTFPPGAKK40 pKa = 9.32LVPIPCGQCIGCRR53 pKa = 11.84LEE55 pKa = 5.86RR56 pKa = 11.84SRR58 pKa = 11.84QWAIRR63 pKa = 11.84LLKK66 pKa = 10.19EE67 pKa = 4.12MKK69 pKa = 10.58LHH71 pKa = 6.32DD72 pKa = 3.82TSSFLTLTYY81 pKa = 10.58DD82 pKa = 4.12DD83 pKa = 3.91KK84 pKa = 11.4HH85 pKa = 7.45LPLVGTSLRR94 pKa = 11.84PTLRR98 pKa = 11.84KK99 pKa = 9.83EE100 pKa = 4.52DD101 pKa = 3.28MTLFLKK107 pKa = 10.6RR108 pKa = 11.84LRR110 pKa = 11.84FAIQPKK116 pKa = 9.85KK117 pKa = 10.43IRR119 pKa = 11.84FFQCGEE125 pKa = 3.8YY126 pKa = 10.92GEE128 pKa = 4.53THH130 pKa = 6.26GRR132 pKa = 11.84PHH134 pKa = 7.08HH135 pKa = 6.33HH136 pKa = 6.79MILFGEE142 pKa = 4.61DD143 pKa = 3.34FVKK146 pKa = 10.7DD147 pKa = 3.38RR148 pKa = 11.84TRR150 pKa = 11.84IRR152 pKa = 11.84DD153 pKa = 3.28SRR155 pKa = 11.84AGFPQYY161 pKa = 10.28TSPLLSRR168 pKa = 11.84LWHH171 pKa = 5.94YY172 pKa = 11.37GEE174 pKa = 4.8AVISEE179 pKa = 4.28VSFEE183 pKa = 4.03SAAYY187 pKa = 8.09VARR190 pKa = 11.84YY191 pKa = 8.77SLKK194 pKa = 9.9KK195 pKa = 10.19HH196 pKa = 6.4LGPGSKK202 pKa = 10.16LMYY205 pKa = 9.77DD206 pKa = 4.06GKK208 pKa = 10.33VPEE211 pKa = 4.45YY212 pKa = 10.28VTMSRR217 pKa = 11.84NPGIAAGYY225 pKa = 8.19FEE227 pKa = 5.74RR228 pKa = 11.84FHH230 pKa = 7.53TDD232 pKa = 3.0MYY234 pKa = 10.67PHH236 pKa = 7.39DD237 pKa = 4.46EE238 pKa = 4.29VVPGPGRR245 pKa = 11.84PASLPPRR252 pKa = 11.84YY253 pKa = 8.99FDD255 pKa = 4.15KK256 pKa = 11.0LLEE259 pKa = 4.39RR260 pKa = 11.84VDD262 pKa = 3.93PNLFAEE268 pKa = 4.37IKK270 pKa = 9.89KK271 pKa = 10.16NRR273 pKa = 11.84TAGIDD278 pKa = 3.79FYY280 pKa = 11.49SDD282 pKa = 3.51PNSTDD287 pKa = 2.54SRR289 pKa = 11.84LATRR293 pKa = 11.84EE294 pKa = 3.88NIKK297 pKa = 8.99TLTVKK302 pKa = 10.57NCLKK306 pKa = 10.07RR307 pKa = 11.84TII309 pKa = 4.07

Molecular weight: 35.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1362

92

540

272.4

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.499 ± 1.677

0.661 ± 0.339

5.36 ± 0.33

5.727 ± 0.811

4.479 ± 0.699

6.314 ± 0.251

2.129 ± 0.302

5.066 ± 0.166

5.066 ± 1.283

7.636 ± 1.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.477

3.671 ± 0.547

7.048 ± 1.156

3.965 ± 0.895

6.681 ± 0.612

6.681 ± 0.385

6.681 ± 0.33

4.772 ± 0.627

1.101 ± 0.232

3.965 ± 0.702
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski