Erythrobacteraceae bacterium CFH 75059

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; unclassified Erythrobacteraceae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0P0Y1|A0A4R0P0Y1_9SPHN Gene transfer agent family protein OS=Erythrobacteraceae bacterium CFH 75059 OX=2528174 GN=EYB45_03990 PE=4 SV=1
MM1 pKa = 6.99TVEE4 pKa = 4.79LLVASAIGVMTAAGVYY20 pKa = 9.8LCLRR24 pKa = 11.84GSTFSVVLGLAMLSYY39 pKa = 10.72AINAFLFSMGRR50 pKa = 11.84LVVDD54 pKa = 4.75RR55 pKa = 11.84PPIYY59 pKa = 10.57DD60 pKa = 3.49PSAPGFTDD68 pKa = 4.24PLPQALVLTAIVIAFGMTALVVVLALRR95 pKa = 11.84SYY97 pKa = 11.05LEE99 pKa = 4.4SGTDD103 pKa = 3.6DD104 pKa = 3.53VNGAGAEE111 pKa = 4.02PPPADD116 pKa = 4.25GDD118 pKa = 4.27APDD121 pKa = 5.45DD122 pKa = 4.07PAEE125 pKa = 4.43PPCTT129 pKa = 3.64

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0NMG5|A0A4R0NMG5_9SPHN Gfo/Idh/MocA family oxidoreductase OS=Erythrobacteraceae bacterium CFH 75059 OX=2528174 GN=EYB45_10315 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 7.95VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 6.26TLCAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2149

0

2149

689682

41

1718

320.9

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.207 ± 0.084

0.856 ± 0.017

5.786 ± 0.037

5.568 ± 0.054

3.403 ± 0.031

8.912 ± 0.049

2.118 ± 0.027

4.196 ± 0.032

1.992 ± 0.041

10.402 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.021

2.202 ± 0.032

5.602 ± 0.039

3.227 ± 0.03

8.616 ± 0.05

4.806 ± 0.031

5.134 ± 0.039

7.455 ± 0.039

1.425 ± 0.021

1.909 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski