Nitrosopumilus spindle-shaped virus

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus; unclassified Alphafusellovirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514K347|A0A514K347_9VIRU Uncharacterized protein OS=Nitrosopumilus spindle-shaped virus OX=2508184 PE=4 SV=1
MM1 pKa = 7.69TEE3 pKa = 4.37MLLQTLDD10 pKa = 3.33NNYY13 pKa = 10.32NDD15 pKa = 5.11DD16 pKa = 3.92PFNLVDD22 pKa = 4.32EE23 pKa = 4.91EE24 pKa = 4.67FNDD27 pKa = 4.2FASKK31 pKa = 10.51HH32 pKa = 6.17IISKK36 pKa = 9.97VIIDD40 pKa = 3.51QGMVHH45 pKa = 6.77IFYY48 pKa = 9.83EE49 pKa = 4.12KK50 pKa = 10.59QEE52 pKa = 4.7GIVCDD57 pKa = 3.76

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514K352|A0A514K352_9VIRU Uncharacterized protein OS=Nitrosopumilus spindle-shaped virus OX=2508184 PE=4 SV=1
MM1 pKa = 7.65NSLTKK6 pKa = 10.61GLIIGGVALGIIFTVGLVAYY26 pKa = 8.69AHH28 pKa = 7.11KK29 pKa = 10.47EE30 pKa = 3.94RR31 pKa = 11.84IVLYY35 pKa = 9.84GLKK38 pKa = 10.35SRR40 pKa = 11.84II41 pKa = 3.67

Molecular weight: 4.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

4426

33

634

147.5

16.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.293 ± 0.449

1.243 ± 0.295

6.281 ± 0.373

7.569 ± 0.774

4.654 ± 0.552

4.948 ± 0.396

1.514 ± 0.258

8.089 ± 0.438

9.67 ± 1.005

8.834 ± 0.651

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.164

6.869 ± 0.547

3.208 ± 0.213

4.067 ± 0.357

3.434 ± 0.325

7.388 ± 0.577

6.371 ± 0.701

5.445 ± 0.366

0.655 ± 0.154

3.525 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski