Amino acid dipepetide frequency for Nitrosopumilus spindle-shaped virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.226AlaCys: 0.226 ± 0.211
1.808AlaAsp: 1.808 ± 0.645
3.842AlaGlu: 3.842 ± 0.864
2.712AlaPhe: 2.712 ± 0.896
2.486AlaGly: 2.486 ± 0.606
0.452AlaHis: 0.452 ± 0.314
3.616AlaIle: 3.616 ± 1.071
2.938AlaLys: 2.938 ± 0.995
5.198AlaLeu: 5.198 ± 1.135
0.678AlaMet: 0.678 ± 0.362
1.356AlaAsn: 1.356 ± 0.491
1.356AlaPro: 1.356 ± 0.564
2.938AlaGln: 2.938 ± 1.074
1.13AlaArg: 1.13 ± 0.515
5.198AlaSer: 5.198 ± 1.323
3.616AlaThr: 3.616 ± 0.941
1.13AlaVal: 1.13 ± 0.541
0.226AlaTrp: 0.226 ± 0.179
2.034AlaTyr: 2.034 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.226CysAla: 0.226 ± 0.179
0.0CysCys: 0.0 ± 0.0
1.13CysAsp: 1.13 ± 0.56
0.904CysGlu: 0.904 ± 0.452
1.13CysPhe: 1.13 ± 0.517
1.582CysGly: 1.582 ± 0.791
0.0CysHis: 0.0 ± 0.0
0.678CysIle: 0.678 ± 0.317
1.582CysLys: 1.582 ± 0.965
1.356CysLeu: 1.356 ± 0.563
0.0CysMet: 0.0 ± 0.0
1.356CysAsn: 1.356 ± 0.659
0.0CysPro: 0.0 ± 0.0
0.226CysGln: 0.226 ± 0.271
0.904CysArg: 0.904 ± 0.413
0.678CysSer: 0.678 ± 0.365
0.0CysThr: 0.0 ± 0.0
0.0CysVal: 0.0 ± 0.0
0.226CysTrp: 0.226 ± 0.2
0.452CysTyr: 0.452 ± 0.275
0.0CysXaa: 0.0 ± 0.0
Asp
4.068AspAla: 4.068 ± 1.031
0.678AspCys: 0.678 ± 0.503
4.746AspAsp: 4.746 ± 0.88
3.616AspGlu: 3.616 ± 0.799
2.486AspPhe: 2.486 ± 0.887
2.26AspGly: 2.26 ± 0.699
1.356AspHis: 1.356 ± 0.641
4.746AspIle: 4.746 ± 1.118
4.972AspLys: 4.972 ± 1.178
7.232AspLeu: 7.232 ± 1.763
0.678AspMet: 0.678 ± 0.425
4.294AspAsn: 4.294 ± 0.818
5.876AspPro: 5.876 ± 1.985
3.164AspGln: 3.164 ± 1.067
1.356AspArg: 1.356 ± 0.458
4.294AspSer: 4.294 ± 0.883
2.712AspThr: 2.712 ± 0.637
1.356AspVal: 1.356 ± 0.554
0.452AspTrp: 0.452 ± 0.327
2.486AspTyr: 2.486 ± 0.642
0.0AspXaa: 0.0 ± 0.0
Glu
1.808GluAla: 1.808 ± 0.869
0.226GluCys: 0.226 ± 0.232
5.198GluAsp: 5.198 ± 1.121
5.876GluGlu: 5.876 ± 1.449
4.294GluPhe: 4.294 ± 0.933
3.616GluGly: 3.616 ± 0.963
0.678GluHis: 0.678 ± 0.41
6.102GluIle: 6.102 ± 1.328
7.458GluLys: 7.458 ± 1.389
8.814GluLeu: 8.814 ± 1.967
2.938GluMet: 2.938 ± 0.701
5.65GluAsn: 5.65 ± 0.941
0.452GluPro: 0.452 ± 0.295
1.356GluGln: 1.356 ± 0.469
0.904GluArg: 0.904 ± 0.429
4.972GluSer: 4.972 ± 1.013
5.198GluThr: 5.198 ± 1.475
7.232GluVal: 7.232 ± 1.504
0.452GluTrp: 0.452 ± 0.336
2.486GluTyr: 2.486 ± 0.757
0.0GluXaa: 0.0 ± 0.0
Phe
3.616PheAla: 3.616 ± 0.765
0.452PheCys: 0.452 ± 0.312
3.842PheAsp: 3.842 ± 1.069
3.616PheGlu: 3.616 ± 0.84
2.034PhePhe: 2.034 ± 0.736
1.808PheGly: 1.808 ± 0.752
1.582PheHis: 1.582 ± 0.59
2.034PheIle: 2.034 ± 0.928
3.616PheLys: 3.616 ± 0.912
3.164PheLeu: 3.164 ± 0.818
0.678PheMet: 0.678 ± 0.313
3.842PheAsn: 3.842 ± 0.796
1.808PhePro: 1.808 ± 0.538
1.13PheGln: 1.13 ± 0.592
1.808PheArg: 1.808 ± 0.527
3.616PheSer: 3.616 ± 0.956
2.486PheThr: 2.486 ± 0.8
2.938PheVal: 2.938 ± 0.626
0.452PheTrp: 0.452 ± 0.316
2.034PheTyr: 2.034 ± 0.783
0.0PheXaa: 0.0 ± 0.0
Gly
0.678GlyAla: 0.678 ± 0.328
0.226GlyCys: 0.226 ± 0.231
4.068GlyAsp: 4.068 ± 0.762
2.26GlyGlu: 2.26 ± 0.703
2.26GlyPhe: 2.26 ± 0.761
2.486GlyGly: 2.486 ± 0.955
1.356GlyHis: 1.356 ± 0.495
4.972GlyIle: 4.972 ± 1.021
6.328GlyLys: 6.328 ± 1.264
3.164GlyLeu: 3.164 ± 0.746
0.904GlyMet: 0.904 ± 0.517
2.26GlyAsn: 2.26 ± 0.621
0.0GlyPro: 0.0 ± 0.0
2.26GlyGln: 2.26 ± 0.631
1.356GlyArg: 1.356 ± 0.628
3.39GlySer: 3.39 ± 0.946
4.972GlyThr: 4.972 ± 1.59
4.972GlyVal: 4.972 ± 1.242
0.226GlyTrp: 0.226 ± 0.179
1.356GlyTyr: 1.356 ± 0.514
0.0GlyXaa: 0.0 ± 0.0
His
1.356HisAla: 1.356 ± 0.496
0.226HisCys: 0.226 ± 0.271
0.678HisAsp: 0.678 ± 0.388
0.904HisGlu: 0.904 ± 0.393
0.226HisPhe: 0.226 ± 0.219
0.226HisGly: 0.226 ± 0.193
0.452HisHis: 0.452 ± 0.297
2.26HisIle: 2.26 ± 0.748
2.034HisLys: 2.034 ± 0.734
0.904HisLeu: 0.904 ± 0.42
0.0HisMet: 0.0 ± 0.0
1.582HisAsn: 1.582 ± 0.719
0.226HisPro: 0.226 ± 0.203
0.452HisGln: 0.452 ± 0.297
0.678HisArg: 0.678 ± 0.326
0.678HisSer: 0.678 ± 0.404
0.904HisThr: 0.904 ± 0.509
0.226HisVal: 0.226 ± 0.211
0.226HisTrp: 0.226 ± 0.179
0.904HisTyr: 0.904 ± 0.456
0.0HisXaa: 0.0 ± 0.0
Ile
3.616IleAla: 3.616 ± 0.737
0.904IleCys: 0.904 ± 0.485
5.65IleAsp: 5.65 ± 1.19
5.198IleGlu: 5.198 ± 1.617
3.842IlePhe: 3.842 ± 0.722
4.294IleGly: 4.294 ± 0.961
0.904IleHis: 0.904 ± 0.621
5.65IleIle: 5.65 ± 1.371
8.588IleLys: 8.588 ± 1.809
5.424IleLeu: 5.424 ± 1.074
1.356IleMet: 1.356 ± 0.526
5.65IleAsn: 5.65 ± 1.338
3.164IlePro: 3.164 ± 0.718
3.616IleGln: 3.616 ± 0.916
2.26IleArg: 2.26 ± 0.63
7.684IleSer: 7.684 ± 1.328
5.65IleThr: 5.65 ± 1.331
5.424IleVal: 5.424 ± 1.252
0.226IleTrp: 0.226 ± 0.203
1.808IleTyr: 1.808 ± 0.616
0.0IleXaa: 0.0 ± 0.0
Lys
2.938LysAla: 2.938 ± 0.701
1.582LysCys: 1.582 ± 0.774
5.876LysAsp: 5.876 ± 0.988
9.718LysGlu: 9.718 ± 1.444
3.39LysPhe: 3.39 ± 0.805
4.52LysGly: 4.52 ± 0.766
0.904LysHis: 0.904 ± 0.449
7.006LysIle: 7.006 ± 1.207
9.266LysLys: 9.266 ± 1.874
9.718LysLeu: 9.718 ± 1.905
3.39LysMet: 3.39 ± 0.759
6.554LysAsn: 6.554 ± 1.516
4.294LysPro: 4.294 ± 1.346
4.068LysGln: 4.068 ± 0.769
3.616LysArg: 3.616 ± 0.817
6.102LysSer: 6.102 ± 1.572
4.972LysThr: 4.972 ± 0.997
4.52LysVal: 4.52 ± 1.081
0.678LysTrp: 0.678 ± 0.382
3.616LysTyr: 3.616 ± 1.093
0.0LysXaa: 0.0 ± 0.0
Leu
3.164LeuAla: 3.164 ± 0.766
2.034LeuCys: 2.034 ± 0.771
6.102LeuAsp: 6.102 ± 1.044
8.136LeuGlu: 8.136 ± 2.271
3.39LeuPhe: 3.39 ± 0.873
3.616LeuGly: 3.616 ± 0.747
1.13LeuHis: 1.13 ± 0.545
6.102LeuIle: 6.102 ± 1.978
11.751LeuLys: 11.751 ± 1.718
4.294LeuLeu: 4.294 ± 0.977
2.26LeuMet: 2.26 ± 0.71
5.65LeuAsn: 5.65 ± 1.134
1.808LeuPro: 1.808 ± 0.858
4.294LeuGln: 4.294 ± 0.803
3.616LeuArg: 3.616 ± 1.033
5.65LeuSer: 5.65 ± 0.87
5.65LeuThr: 5.65 ± 1.026
4.52LeuVal: 4.52 ± 1.223
0.678LeuTrp: 0.678 ± 0.341
4.52LeuTyr: 4.52 ± 1.123
0.0LeuXaa: 0.0 ± 0.0
Met
0.678MetAla: 0.678 ± 0.41
0.226MetCys: 0.226 ± 0.271
0.904MetAsp: 0.904 ± 0.45
0.904MetGlu: 0.904 ± 0.597
1.13MetPhe: 1.13 ± 0.434
1.356MetGly: 1.356 ± 0.576
0.0MetHis: 0.0 ± 0.0
2.034MetIle: 2.034 ± 0.667
1.356MetLys: 1.356 ± 0.476
1.356MetLeu: 1.356 ± 0.497
0.452MetMet: 0.452 ± 0.27
1.356MetAsn: 1.356 ± 0.451
0.678MetPro: 0.678 ± 0.543
0.452MetGln: 0.452 ± 0.357
1.356MetArg: 1.356 ± 0.543
1.582MetSer: 1.582 ± 0.587
1.356MetThr: 1.356 ± 0.464
2.034MetVal: 2.034 ± 0.72
0.0MetTrp: 0.0 ± 0.0
0.226MetTyr: 0.226 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.52AsnAla: 4.52 ± 1.67
1.13AsnCys: 1.13 ± 0.567
4.972AsnAsp: 4.972 ± 1.145
4.746AsnGlu: 4.746 ± 1.166
2.486AsnPhe: 2.486 ± 0.727
4.746AsnGly: 4.746 ± 0.981
0.678AsnHis: 0.678 ± 0.347
7.232AsnIle: 7.232 ± 1.481
4.294AsnLys: 4.294 ± 1.192
6.554AsnLeu: 6.554 ± 1.06
0.452AsnMet: 0.452 ± 0.387
3.616AsnAsn: 3.616 ± 1.184
2.712AsnPro: 2.712 ± 0.544
4.294AsnGln: 4.294 ± 1.516
2.486AsnArg: 2.486 ± 0.581
5.198AsnSer: 5.198 ± 1.139
3.164AsnThr: 3.164 ± 0.725
3.616AsnVal: 3.616 ± 0.933
0.452AsnTrp: 0.452 ± 0.309
1.13AsnTyr: 1.13 ± 0.556
0.0AsnXaa: 0.0 ± 0.0
Pro
0.452ProAla: 0.452 ± 0.286
0.226ProCys: 0.226 ± 0.2
1.582ProAsp: 1.582 ± 0.781
2.712ProGlu: 2.712 ± 0.622
1.582ProPhe: 1.582 ± 0.492
0.0ProGly: 0.0 ± 0.0
0.226ProHis: 0.226 ± 0.248
3.842ProIle: 3.842 ± 1.19
2.712ProLys: 2.712 ± 0.669
2.486ProLeu: 2.486 ± 0.804
0.0ProMet: 0.0 ± 0.0
2.486ProAsn: 2.486 ± 0.567
0.0ProPro: 0.0 ± 0.0
0.904ProGln: 0.904 ± 0.399
0.904ProArg: 0.904 ± 0.406
4.746ProSer: 4.746 ± 1.266
3.164ProThr: 3.164 ± 0.732
2.034ProVal: 2.034 ± 0.529
0.0ProTrp: 0.0 ± 0.0
2.034ProTyr: 2.034 ± 0.885
0.0ProXaa: 0.0 ± 0.0
Gln
2.26GlnAla: 2.26 ± 0.887
0.0GlnCys: 0.0 ± 0.0
2.938GlnAsp: 2.938 ± 0.677
2.712GlnGlu: 2.712 ± 1.333
1.356GlnPhe: 1.356 ± 0.468
1.356GlnGly: 1.356 ± 0.494
0.226GlnHis: 0.226 ± 0.225
3.39GlnIle: 3.39 ± 0.813
3.616GlnLys: 3.616 ± 0.912
2.938GlnLeu: 2.938 ± 0.788
1.13GlnMet: 1.13 ± 0.389
3.616GlnAsn: 3.616 ± 0.735
1.356GlnPro: 1.356 ± 0.566
2.034GlnGln: 2.034 ± 0.67
1.13GlnArg: 1.13 ± 0.441
1.808GlnSer: 1.808 ± 0.595
5.424GlnThr: 5.424 ± 1.017
1.808GlnVal: 1.808 ± 0.723
0.452GlnTrp: 0.452 ± 0.267
1.13GlnTyr: 1.13 ± 0.481
0.0GlnXaa: 0.0 ± 0.0
Arg
0.904ArgAla: 0.904 ± 0.333
0.678ArgCys: 0.678 ± 0.411
1.582ArgAsp: 1.582 ± 0.562
3.164ArgGlu: 3.164 ± 0.956
1.808ArgPhe: 1.808 ± 0.637
1.808ArgGly: 1.808 ± 0.563
0.678ArgHis: 0.678 ± 0.358
2.486ArgIle: 2.486 ± 0.649
3.39ArgLys: 3.39 ± 1.033
3.164ArgLeu: 3.164 ± 0.646
0.904ArgMet: 0.904 ± 0.43
2.712ArgAsn: 2.712 ± 0.747
0.678ArgPro: 0.678 ± 0.316
1.582ArgGln: 1.582 ± 0.596
1.582ArgArg: 1.582 ± 0.461
2.712ArgSer: 2.712 ± 1.122
1.808ArgThr: 1.808 ± 0.741
1.582ArgVal: 1.582 ± 0.752
0.226ArgTrp: 0.226 ± 0.2
0.904ArgTyr: 0.904 ± 0.481
0.0ArgXaa: 0.0 ± 0.0
Ser
3.842SerAla: 3.842 ± 1.03
0.904SerCys: 0.904 ± 0.528
3.616SerAsp: 3.616 ± 0.743
5.876SerGlu: 5.876 ± 1.167
4.294SerPhe: 4.294 ± 1.049
5.198SerGly: 5.198 ± 1.371
0.678SerHis: 0.678 ± 0.491
5.65SerIle: 5.65 ± 0.788
8.588SerLys: 8.588 ± 1.818
6.78SerLeu: 6.78 ± 1.352
0.904SerMet: 0.904 ± 0.483
5.876SerAsn: 5.876 ± 1.318
2.26SerPro: 2.26 ± 0.556
1.356SerGln: 1.356 ± 0.592
2.486SerArg: 2.486 ± 0.711
5.876SerSer: 5.876 ± 1.438
3.39SerThr: 3.39 ± 0.773
3.164SerVal: 3.164 ± 0.826
0.226SerTrp: 0.226 ± 0.211
2.938SerTyr: 2.938 ± 0.616
0.0SerXaa: 0.0 ± 0.0
Thr
4.068ThrAla: 4.068 ± 1.204
0.904ThrCys: 0.904 ± 0.367
4.52ThrAsp: 4.52 ± 1.551
5.198ThrGlu: 5.198 ± 1.037
3.842ThrPhe: 3.842 ± 1.168
2.486ThrGly: 2.486 ± 0.716
2.26ThrHis: 2.26 ± 0.685
4.52ThrIle: 4.52 ± 0.962
5.198ThrLys: 5.198 ± 1.013
5.876ThrLeu: 5.876 ± 0.988
0.678ThrMet: 0.678 ± 0.501
3.616ThrAsn: 3.616 ± 0.798
2.26ThrPro: 2.26 ± 0.924
2.712ThrGln: 2.712 ± 1.252
2.712ThrArg: 2.712 ± 0.771
3.842ThrSer: 3.842 ± 1.157
4.068ThrThr: 4.068 ± 1.311
2.938ThrVal: 2.938 ± 1.104
0.0ThrTrp: 0.0 ± 0.0
2.034ThrTyr: 2.034 ± 0.571
0.0ThrXaa: 0.0 ± 0.0
Val
1.808ValAla: 1.808 ± 0.791
1.13ValCys: 1.13 ± 0.464
2.486ValAsp: 2.486 ± 0.669
2.938ValGlu: 2.938 ± 0.702
1.13ValPhe: 1.13 ± 0.459
2.486ValGly: 2.486 ± 0.819
0.904ValHis: 0.904 ± 0.482
6.328ValIle: 6.328 ± 1.377
6.102ValLys: 6.102 ± 1.227
5.876ValLeu: 5.876 ± 0.986
1.356ValMet: 1.356 ± 0.591
3.39ValAsn: 3.39 ± 0.7
2.034ValPro: 2.034 ± 0.422
1.356ValGln: 1.356 ± 0.549
3.164ValArg: 3.164 ± 0.931
3.164ValSer: 3.164 ± 0.777
3.616ValThr: 3.616 ± 1.249
2.938ValVal: 2.938 ± 0.829
0.452ValTrp: 0.452 ± 0.301
1.808ValTyr: 1.808 ± 0.536
0.0ValXaa: 0.0 ± 0.0
Trp
0.452TrpAla: 0.452 ± 0.302
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
0.452TrpGlu: 0.452 ± 0.27
0.452TrpPhe: 0.452 ± 0.288
0.0TrpGly: 0.0 ± 0.0
0.226TrpHis: 0.226 ± 0.203
0.678TrpIle: 0.678 ± 0.419
0.452TrpLys: 0.452 ± 0.269
0.452TrpLeu: 0.452 ± 0.266
0.226TrpMet: 0.226 ± 0.203
1.808TrpAsn: 1.808 ± 0.772
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.0TrpArg: 0.0 ± 0.0
0.452TrpSer: 0.452 ± 0.319
0.452TrpThr: 0.452 ± 0.407
0.226TrpVal: 0.226 ± 0.179
0.0TrpTrp: 0.0 ± 0.0
0.226TrpTyr: 0.226 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.486TyrAla: 2.486 ± 0.808
0.678TyrCys: 0.678 ± 0.361
1.13TyrAsp: 1.13 ± 0.595
2.938TyrGlu: 2.938 ± 0.79
2.712TyrPhe: 2.712 ± 0.99
3.164TyrGly: 3.164 ± 0.611
0.452TyrHis: 0.452 ± 0.275
1.582TyrIle: 1.582 ± 0.488
2.486TyrLys: 2.486 ± 0.735
3.616TyrLeu: 3.616 ± 0.957
0.226TyrMet: 0.226 ± 0.179
2.034TyrAsn: 2.034 ± 0.656
1.13TyrPro: 1.13 ± 0.605
2.486TyrGln: 2.486 ± 0.603
0.904TyrArg: 0.904 ± 0.445
2.26TyrSer: 2.26 ± 0.682
1.13TyrThr: 1.13 ± 0.549
1.808TyrVal: 1.808 ± 0.645
0.904TyrTrp: 0.904 ± 0.427
1.13TyrTyr: 1.13 ± 0.399
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 30 proteins (4426 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski