Turkey aviadenovirus 5
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5NEG4|U5NEG4_9ADEN ORF17 OS=Turkey aviadenovirus 5 OX=1408258 PE=4 SV=1
MM1 pKa = 7.26 MNDD4 pKa = 3.09 EE5 pKa = 4.44 QLAEE9 pKa = 4.57 LLQQAPDD16 pKa = 3.99 DD17 pKa = 4.69 GEE19 pKa = 4.47 DD20 pKa = 3.55 EE21 pKa = 4.32 NGEE24 pKa = 4.29 LEE26 pKa = 4.46 MPAALHH32 pKa = 7.31 AYY34 pKa = 9.5 QLLCFEE40 pKa = 5.55 SYY42 pKa = 10.54 AIHH45 pKa = 6.74 VGSCSCHH52 pKa = 6.02 SLQLTRR58 pKa = 11.84 LGLSACHH65 pKa = 5.79 TAPSDD70 pKa = 3.19 LATYY74 pKa = 9.59 VWARR78 pKa = 11.84 LQDD81 pKa = 3.85 DD82 pKa = 4.21 FNVAGLYY89 pKa = 9.02 FVAMWACPAFRR100 pKa = 11.84 DD101 pKa = 4.69 FSPVFMSRR109 pKa = 11.84 AVGRR113 pKa = 11.84 NEE115 pKa = 4.61 DD116 pKa = 3.69 VLGMTIHH123 pKa = 6.36 VGDD126 pKa = 4.07 AARR129 pKa = 11.84 LAFLIAVSRR138 pKa = 11.84 MGEE141 pKa = 3.98 AGASPCLYY149 pKa = 8.56 MRR151 pKa = 11.84 KK152 pKa = 8.71 MDD154 pKa = 3.78 IDD156 pKa = 3.66 VEE158 pKa = 4.33 EE159 pKa = 4.46 QVVHH163 pKa = 6.42 MFTDD167 pKa = 3.35 DD168 pKa = 3.4 WFSEE172 pKa = 4.42 FANLLYY178 pKa = 10.41 YY179 pKa = 9.04 WQAAEE184 pKa = 3.55 WRR186 pKa = 11.84 FINEE190 pKa = 3.67 QKK192 pKa = 11.09
Molecular weight: 21.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.118
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.164
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.19
Rodwell 4.177
Grimsley 4.075
Solomon 4.291
Lehninger 4.253
Nozaki 4.406
DTASelect 4.584
Thurlkill 4.19
EMBOSS 4.202
Sillero 4.457
Patrickios 1.24
IPC_peptide 4.291
IPC2_peptide 4.444
IPC2.peptide.svr19 4.33
Protein with the highest isoelectric point:
>tr|U5NEL9|U5NEL9_9ADEN DNA polymerase OS=Turkey aviadenovirus 5 OX=1408258 PE=3 SV=1
MM1 pKa = 7.45 SVLISPSDD9 pKa = 3.51 NRR11 pKa = 11.84 GWGAGMRR18 pKa = 11.84 YY19 pKa = 9.22 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 TSMRR26 pKa = 11.84 GVGRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 MTLRR36 pKa = 11.84 QLLGLGARR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 TRR50 pKa = 11.84 PTTVSNRR57 pKa = 11.84 LVVVSTRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 SSRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 3.37
Molecular weight: 8.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.487
IPC2_protein 11.184
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.062
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
1
37
13304
46
1247
359.6
40.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.434 ± 0.336
1.849 ± 0.241
5.622 ± 0.209
5.562 ± 0.502
4.142 ± 0.266
6.374 ± 0.441
2.21 ± 0.235
4.307 ± 0.252
3.751 ± 0.263
9.313 ± 0.353
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.3 ± 0.167
4.262 ± 0.368
6.254 ± 0.288
3.593 ± 0.203
7.118 ± 0.484
7.426 ± 0.299
6.562 ± 0.421
6.622 ± 0.212
1.473 ± 0.147
3.826 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here