Serratia sp. S1B
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6810 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1HS46|A0A2V1HS46_9GAMM WYL domain-containing protein OS=Serratia sp. S1B OX=2233853 GN=C9426_01935 PE=4 SV=1
MM1 pKa = 7.59 TCTSCACNDD10 pKa = 3.64 LVLEE14 pKa = 4.51 LLQILKK20 pKa = 10.44 AQIQSQAEE28 pKa = 4.22 QNNLLAQIIDD38 pKa = 3.71 QNNDD42 pKa = 3.77 LIASLCDD49 pKa = 3.34 DD50 pKa = 3.88 TDD52 pKa = 3.95 DD53 pKa = 4.34 KK54 pKa = 11.53 RR55 pKa = 11.84 EE56 pKa = 3.91 RR57 pKa = 11.84 YY58 pKa = 9.94 LDD60 pKa = 4.06 DD61 pKa = 3.6 EE62 pKa = 4.55
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.745
IPC_protein 3.681
Toseland 3.465
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.808
Patrickios 1.875
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A2V1HD07|A0A2V1HD07_9GAMM Penicillin-insensitive transglycosylase OS=Serratia sp. S1B OX=2233853 GN=pbpC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSKK46 pKa = 10.95
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6810
0
6810
2172407
15
6903
319.0
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.224 ± 0.033
1.067 ± 0.011
4.989 ± 0.024
5.486 ± 0.037
3.955 ± 0.022
7.058 ± 0.036
2.419 ± 0.019
6.244 ± 0.026
4.573 ± 0.03
10.93 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.582 ± 0.017
4.099 ± 0.039
4.252 ± 0.019
5.451 ± 0.032
4.942 ± 0.031
6.068 ± 0.03
5.37 ± 0.04
6.909 ± 0.024
1.355 ± 0.013
3.028 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here