Amino acid dipepetide frequency for Serratia sp. S1B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.868AlaAla: 8.868 ± 0.086
1.102AlaCys: 1.102 ± 0.023
4.675AlaAsp: 4.675 ± 0.059
5.717AlaGlu: 5.717 ± 0.06
3.416AlaPhe: 3.416 ± 0.044
6.835AlaGly: 6.835 ± 0.078
1.923AlaHis: 1.923 ± 0.035
5.98AlaIle: 5.98 ± 0.058
4.397AlaLys: 4.397 ± 0.058
10.77AlaLeu: 10.77 ± 0.083
2.651AlaMet: 2.651 ± 0.035
3.524AlaAsn: 3.524 ± 0.057
3.26AlaPro: 3.26 ± 0.045
4.807AlaGln: 4.807 ± 0.051
4.262AlaArg: 4.262 ± 0.058
5.21AlaSer: 5.21 ± 0.054
4.753AlaThr: 4.753 ± 0.067
6.366AlaVal: 6.366 ± 0.065
1.252AlaTrp: 1.252 ± 0.029
2.476AlaTyr: 2.476 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.884CysAla: 0.884 ± 0.023
0.172CysCys: 0.172 ± 0.009
0.549CysAsp: 0.549 ± 0.017
0.541CysGlu: 0.541 ± 0.017
0.464CysPhe: 0.464 ± 0.015
0.974CysGly: 0.974 ± 0.026
0.328CysHis: 0.328 ± 0.012
0.664CysIle: 0.664 ± 0.018
0.365CysLys: 0.365 ± 0.014
1.091CysLeu: 1.091 ± 0.024
0.228CysMet: 0.228 ± 0.012
0.334CysAsn: 0.334 ± 0.014
0.489CysPro: 0.489 ± 0.017
0.574CysGln: 0.574 ± 0.018
0.574CysArg: 0.574 ± 0.02
0.705CysSer: 0.705 ± 0.016
0.49CysThr: 0.49 ± 0.015
0.709CysVal: 0.709 ± 0.021
0.166CysTrp: 0.166 ± 0.009
0.364CysTyr: 0.364 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
4.572AspAla: 4.572 ± 0.053
0.498AspCys: 0.498 ± 0.016
2.593AspAsp: 2.593 ± 0.04
3.147AspGlu: 3.147 ± 0.043
2.145AspPhe: 2.145 ± 0.03
3.347AspGly: 3.347 ± 0.051
1.198AspHis: 1.198 ± 0.023
3.433AspIle: 3.433 ± 0.04
2.334AspLys: 2.334 ± 0.033
5.08AspLeu: 5.08 ± 0.054
1.253AspMet: 1.253 ± 0.027
2.058AspAsn: 2.058 ± 0.038
2.183AspPro: 2.183 ± 0.038
2.254AspGln: 2.254 ± 0.036
2.369AspArg: 2.369 ± 0.035
2.833AspSer: 2.833 ± 0.042
2.386AspThr: 2.386 ± 0.033
3.507AspVal: 3.507 ± 0.044
0.804AspTrp: 0.804 ± 0.021
1.897AspTyr: 1.897 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
4.723GluAla: 4.723 ± 0.056
0.452GluCys: 0.452 ± 0.016
2.316GluAsp: 2.316 ± 0.034
2.878GluGlu: 2.878 ± 0.044
1.995GluPhe: 1.995 ± 0.036
3.233GluGly: 3.233 ± 0.044
1.559GluHis: 1.559 ± 0.026
3.524GluIle: 3.524 ± 0.041
3.183GluLys: 3.183 ± 0.048
6.069GluLeu: 6.069 ± 0.056
1.589GluMet: 1.589 ± 0.029
2.377GluAsn: 2.377 ± 0.037
1.885GluPro: 1.885 ± 0.037
4.293GluGln: 4.293 ± 0.057
3.29GluArg: 3.29 ± 0.052
2.821GluSer: 2.821 ± 0.039
2.686GluThr: 2.686 ± 0.037
3.749GluVal: 3.749 ± 0.047
0.715GluTrp: 0.715 ± 0.018
1.517GluTyr: 1.517 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.706PheAla: 3.706 ± 0.041
0.533PheCys: 0.533 ± 0.016
2.384PheAsp: 2.384 ± 0.036
2.045PheGlu: 2.045 ± 0.032
1.719PhePhe: 1.719 ± 0.033
3.136PheGly: 3.136 ± 0.044
0.852PheHis: 0.852 ± 0.019
2.711PheIle: 2.711 ± 0.039
1.717PheLys: 1.717 ± 0.03
3.498PheLeu: 3.498 ± 0.045
1.029PheMet: 1.029 ± 0.022
1.999PheAsn: 1.999 ± 0.038
1.466PhePro: 1.466 ± 0.027
1.389PheGln: 1.389 ± 0.028
1.607PheArg: 1.607 ± 0.029
3.056PheSer: 3.056 ± 0.038
2.275PheThr: 2.275 ± 0.037
2.542PheVal: 2.542 ± 0.035
0.567PheTrp: 0.567 ± 0.018
1.316PheTyr: 1.316 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
5.791GlyAla: 5.791 ± 0.087
0.923GlyCys: 0.923 ± 0.024
3.321GlyAsp: 3.321 ± 0.053
3.99GlyGlu: 3.99 ± 0.055
3.168GlyPhe: 3.168 ± 0.039
5.165GlyGly: 5.165 ± 0.085
1.683GlyHis: 1.683 ± 0.029
4.95GlyIle: 4.95 ± 0.06
3.741GlyLys: 3.741 ± 0.047
7.39GlyLeu: 7.39 ± 0.068
2.086GlyMet: 2.086 ± 0.031
2.851GlyAsn: 2.851 ± 0.074
1.737GlyPro: 1.737 ± 0.029
3.272GlyGln: 3.272 ± 0.042
3.321GlyArg: 3.321 ± 0.042
4.24GlySer: 4.24 ± 0.085
3.898GlyThr: 3.898 ± 0.092
5.4GlyVal: 5.4 ± 0.053
1.092GlyTrp: 1.092 ± 0.025
2.563GlyTyr: 2.563 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.033HisAla: 2.033 ± 0.035
0.35HisCys: 0.35 ± 0.014
1.197HisAsp: 1.197 ± 0.025
1.166HisGlu: 1.166 ± 0.022
1.123HisPhe: 1.123 ± 0.025
1.781HisGly: 1.781 ± 0.029
0.852HisHis: 0.852 ± 0.025
1.522HisIle: 1.522 ± 0.027
0.843HisLys: 0.843 ± 0.02
2.552HisLeu: 2.552 ± 0.037
0.554HisMet: 0.554 ± 0.016
0.921HisAsn: 0.921 ± 0.022
1.391HisPro: 1.391 ± 0.027
1.455HisGln: 1.455 ± 0.028
1.184HisArg: 1.184 ± 0.027
1.365HisSer: 1.365 ± 0.028
1.128HisThr: 1.128 ± 0.021
1.38HisVal: 1.38 ± 0.025
0.419HisTrp: 0.419 ± 0.014
0.973HisTyr: 0.973 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.453IleAla: 6.453 ± 0.059
0.727IleCys: 0.727 ± 0.02
3.745IleAsp: 3.745 ± 0.045
3.843IleGlu: 3.843 ± 0.046
2.338IlePhe: 2.338 ± 0.041
4.744IleGly: 4.744 ± 0.053
1.357IleHis: 1.357 ± 0.026
3.606IleIle: 3.606 ± 0.045
2.774IleLys: 2.774 ± 0.045
5.795IleLeu: 5.795 ± 0.064
1.34IleMet: 1.34 ± 0.027
2.814IleAsn: 2.814 ± 0.045
2.824IlePro: 2.824 ± 0.033
2.743IleGln: 2.743 ± 0.042
2.943IleArg: 2.943 ± 0.038
4.177IleSer: 4.177 ± 0.052
3.67IleThr: 3.67 ± 0.047
4.061IleVal: 4.061 ± 0.043
0.681IleTrp: 0.681 ± 0.019
1.81IleTyr: 1.81 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.249LysAla: 4.249 ± 0.058
0.273LysCys: 0.273 ± 0.013
2.152LysAsp: 2.152 ± 0.038
2.419LysGlu: 2.419 ± 0.041
1.444LysPhe: 1.444 ± 0.028
2.746LysGly: 2.746 ± 0.042
1.044LysHis: 1.044 ± 0.022
2.995LysIle: 2.995 ± 0.047
2.558LysLys: 2.558 ± 0.039
4.691LysLeu: 4.691 ± 0.059
1.309LysMet: 1.309 ± 0.026
2.125LysAsn: 2.125 ± 0.038
2.145LysPro: 2.145 ± 0.039
2.823LysGln: 2.823 ± 0.036
2.458LysArg: 2.458 ± 0.043
2.528LysSer: 2.528 ± 0.037
2.726LysThr: 2.726 ± 0.037
3.28LysVal: 3.28 ± 0.046
0.491LysTrp: 0.491 ± 0.014
1.275LysTyr: 1.275 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
10.977LeuAla: 10.977 ± 0.079
1.264LeuCys: 1.264 ± 0.028
5.454LeuAsp: 5.454 ± 0.045
5.588LeuGlu: 5.588 ± 0.06
4.273LeuPhe: 4.273 ± 0.048
7.444LeuGly: 7.444 ± 0.068
2.399LeuHis: 2.399 ± 0.034
6.271LeuIle: 6.271 ± 0.067
5.183LeuLys: 5.183 ± 0.065
11.943LeuLeu: 11.943 ± 0.119
2.799LeuMet: 2.799 ± 0.037
4.769LeuAsn: 4.769 ± 0.073
5.513LeuPro: 5.513 ± 0.063
5.275LeuGln: 5.275 ± 0.064
5.281LeuArg: 5.281 ± 0.061
7.516LeuSer: 7.516 ± 0.064
6.247LeuThr: 6.247 ± 0.097
7.006LeuVal: 7.006 ± 0.067
1.346LeuTrp: 1.346 ± 0.032
2.75LeuTyr: 2.75 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.578MetAla: 2.578 ± 0.039
0.203MetCys: 0.203 ± 0.009
1.076MetAsp: 1.076 ± 0.021
1.039MetGlu: 1.039 ± 0.021
0.861MetPhe: 0.861 ± 0.021
1.808MetGly: 1.808 ± 0.031
0.546MetHis: 0.546 ± 0.017
1.527MetIle: 1.527 ± 0.029
1.299MetLys: 1.299 ± 0.025
2.979MetLeu: 2.979 ± 0.041
0.805MetMet: 0.805 ± 0.02
1.097MetAsn: 1.097 ± 0.023
1.261MetPro: 1.261 ± 0.023
1.389MetGln: 1.389 ± 0.028
1.277MetArg: 1.277 ± 0.028
1.849MetSer: 1.849 ± 0.03
1.615MetThr: 1.615 ± 0.024
1.823MetVal: 1.823 ± 0.03
0.245MetTrp: 0.245 ± 0.012
0.539MetTyr: 0.539 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.548AsnAla: 3.548 ± 0.061
0.389AsnCys: 0.389 ± 0.013
2.005AsnAsp: 2.005 ± 0.035
1.959AsnGlu: 1.959 ± 0.029
1.572AsnPhe: 1.572 ± 0.03
3.088AsnGly: 3.088 ± 0.084
0.98AsnHis: 0.98 ± 0.021
2.767AsnIle: 2.767 ± 0.045
1.809AsnLys: 1.809 ± 0.029
4.108AsnLeu: 4.108 ± 0.07
0.986AsnMet: 0.986 ± 0.023
2.005AsnAsn: 2.005 ± 0.061
2.193AsnPro: 2.193 ± 0.036
2.19AsnGln: 2.19 ± 0.036
1.915AsnArg: 1.915 ± 0.028
2.558AsnSer: 2.558 ± 0.059
2.336AsnThr: 2.336 ± 0.062
2.589AsnVal: 2.589 ± 0.043
0.576AsnTrp: 0.576 ± 0.016
1.415AsnTyr: 1.415 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
3.93ProAla: 3.93 ± 0.047
0.37ProCys: 0.37 ± 0.013
2.376ProAsp: 2.376 ± 0.033
3.233ProGlu: 3.233 ± 0.045
1.769ProPhe: 1.769 ± 0.031
2.564ProGly: 2.564 ± 0.038
1.001ProHis: 1.001 ± 0.023
2.409ProIle: 2.409 ± 0.036
1.861ProLys: 1.861 ± 0.036
4.696ProLeu: 4.696 ± 0.062
1.051ProMet: 1.051 ± 0.021
1.665ProAsn: 1.665 ± 0.03
1.48ProPro: 1.48 ± 0.032
2.337ProGln: 2.337 ± 0.04
1.693ProArg: 1.693 ± 0.031
2.422ProSer: 2.422 ± 0.038
2.258ProThr: 2.258 ± 0.035
3.434ProVal: 3.434 ± 0.05
0.604ProTrp: 0.604 ± 0.019
1.367ProTyr: 1.367 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.336GlnAla: 5.336 ± 0.063
0.428GlnCys: 0.428 ± 0.014
2.303GlnAsp: 2.303 ± 0.04
2.537GlnGlu: 2.537 ± 0.042
1.888GlnPhe: 1.888 ± 0.033
3.587GlnGly: 3.587 ± 0.045
1.759GlnHis: 1.759 ± 0.036
3.051GlnIle: 3.051 ± 0.038
2.219GlnLys: 2.219 ± 0.033
6.241GlnLeu: 6.241 ± 0.072
1.231GlnMet: 1.231 ± 0.022
1.807GlnAsn: 1.807 ± 0.03
2.512GlnPro: 2.512 ± 0.045
5.146GlnGln: 5.146 ± 0.093
3.22GlnArg: 3.22 ± 0.047
2.732GlnSer: 2.732 ± 0.043
2.536GlnThr: 2.536 ± 0.037
3.677GlnVal: 3.677 ± 0.046
0.755GlnTrp: 0.755 ± 0.019
1.54GlnTyr: 1.54 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
3.907ArgAla: 3.907 ± 0.044
0.519ArgCys: 0.519 ± 0.017
2.538ArgAsp: 2.538 ± 0.035
3.091ArgGlu: 3.091 ± 0.042
2.258ArgPhe: 2.258 ± 0.032
2.935ArgGly: 2.935 ± 0.041
1.36ArgHis: 1.36 ± 0.026
3.222ArgIle: 3.222 ± 0.033
2.181ArgLys: 2.181 ± 0.036
5.748ArgLeu: 5.748 ± 0.064
1.283ArgMet: 1.283 ± 0.028
1.862ArgAsn: 1.862 ± 0.028
1.893ArgPro: 1.893 ± 0.032
2.967ArgGln: 2.967 ± 0.039
2.813ArgArg: 2.813 ± 0.045
2.626ArgSer: 2.626 ± 0.038
2.158ArgThr: 2.158 ± 0.029
3.362ArgVal: 3.362 ± 0.041
0.751ArgTrp: 0.751 ± 0.022
1.946ArgTyr: 1.946 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
5.423SerAla: 5.423 ± 0.051
0.632SerCys: 0.632 ± 0.02
2.899SerAsp: 2.899 ± 0.039
3.181SerGlu: 3.181 ± 0.041
2.525SerPhe: 2.525 ± 0.034
4.986SerGly: 4.986 ± 0.084
1.5SerHis: 1.5 ± 0.03
3.626SerIle: 3.626 ± 0.043
2.599SerLys: 2.599 ± 0.039
6.773SerLeu: 6.773 ± 0.065
1.546SerMet: 1.546 ± 0.027
2.459SerAsn: 2.459 ± 0.05
2.547SerPro: 2.547 ± 0.032
2.984SerGln: 2.984 ± 0.044
2.896SerArg: 2.896 ± 0.037
3.991SerSer: 3.991 ± 0.059
3.235SerThr: 3.235 ± 0.056
4.124SerVal: 4.124 ± 0.049
0.834SerTrp: 0.834 ± 0.022
1.917SerTyr: 1.917 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
5.086ThrAla: 5.086 ± 0.084
0.494ThrCys: 0.494 ± 0.018
2.575ThrAsp: 2.575 ± 0.045
2.756ThrGlu: 2.756 ± 0.037
1.977ThrPhe: 1.977 ± 0.03
4.371ThrGly: 4.371 ± 0.081
1.278ThrHis: 1.278 ± 0.025
3.082ThrIle: 3.082 ± 0.039
1.802ThrLys: 1.802 ± 0.029
6.964ThrLeu: 6.964 ± 0.085
1.041ThrMet: 1.041 ± 0.022
1.811ThrAsn: 1.811 ± 0.052
2.961ThrPro: 2.961 ± 0.038
2.64ThrGln: 2.64 ± 0.04
2.493ThrArg: 2.493 ± 0.037
3.051ThrSer: 3.051 ± 0.046
3.238ThrThr: 3.238 ± 0.067
3.958ThrVal: 3.958 ± 0.071
0.729ThrTrp: 0.729 ± 0.018
1.397ThrTyr: 1.397 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
6.601ValAla: 6.601 ± 0.068
0.768ValCys: 0.768 ± 0.019
3.632ValAsp: 3.632 ± 0.046
3.967ValGlu: 3.967 ± 0.058
2.601ValPhe: 2.601 ± 0.037
4.806ValGly: 4.806 ± 0.056
1.324ValHis: 1.324 ± 0.025
4.707ValIle: 4.707 ± 0.052
3.248ValLys: 3.248 ± 0.044
7.318ValLeu: 7.318 ± 0.065
2.048ValMet: 2.048 ± 0.034
2.916ValAsn: 2.916 ± 0.052
2.797ValPro: 2.797 ± 0.039
2.798ValGln: 2.798 ± 0.038
3.217ValArg: 3.217 ± 0.045
4.299ValSer: 4.299 ± 0.049
4.06ValThr: 4.06 ± 0.054
5.34ValVal: 5.34 ± 0.059
0.833ValTrp: 0.833 ± 0.02
1.814ValTyr: 1.814 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
0.932TrpAla: 0.932 ± 0.024
0.181TrpCys: 0.181 ± 0.009
0.569TrpAsp: 0.569 ± 0.017
0.501TrpGlu: 0.501 ± 0.017
0.644TrpPhe: 0.644 ± 0.018
0.82TrpGly: 0.82 ± 0.02
0.406TrpHis: 0.406 ± 0.015
0.75TrpIle: 0.75 ± 0.021
0.508TrpLys: 0.508 ± 0.015
2.076TrpLeu: 2.076 ± 0.039
0.399TrpMet: 0.399 ± 0.012
0.485TrpAsn: 0.485 ± 0.016
0.493TrpPro: 0.493 ± 0.016
1.125TrpGln: 1.125 ± 0.023
0.79TrpArg: 0.79 ± 0.022
0.795TrpSer: 0.795 ± 0.022
0.505TrpThr: 0.505 ± 0.014
0.931TrpVal: 0.931 ± 0.021
0.224TrpTrp: 0.224 ± 0.01
0.418TrpTyr: 0.418 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.648TyrAla: 2.648 ± 0.036
0.39TyrCys: 0.39 ± 0.013
1.53TyrAsp: 1.53 ± 0.028
1.259TyrGlu: 1.259 ± 0.026
1.364TyrPhe: 1.364 ± 0.027
2.211TyrGly: 2.211 ± 0.031
0.84TyrHis: 0.84 ± 0.022
1.645TyrIle: 1.645 ± 0.027
1.108TyrLys: 1.108 ± 0.024
3.516TyrLeu: 3.516 ± 0.043
0.591TyrMet: 0.591 ± 0.017
1.108TyrAsn: 1.108 ± 0.022
1.489TyrPro: 1.489 ± 0.025
2.045TyrGln: 2.045 ± 0.033
1.815TyrArg: 1.815 ± 0.031
1.902TyrSer: 1.902 ± 0.032
1.504TyrThr: 1.504 ± 0.03
1.856TyrVal: 1.856 ± 0.028
0.47TyrTrp: 0.47 ± 0.015
0.986TyrTyr: 0.986 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6810 proteins (2172407 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski