Paraoceanicella profunda
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4636 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8G0V5|A0A5B8G0V5_9RHOB Carbon-nitrogen hydrolase family protein OS=Paraoceanicella profunda OX=2579971 GN=FDP22_09745 PE=3 SV=1
MM1 pKa = 7.51 PAVAQDD7 pKa = 3.54 NAAQANNPLADD18 pKa = 3.61 TTALNFQNQYY28 pKa = 9.98 TGDD31 pKa = 3.61 LTGTDD36 pKa = 3.6 DD37 pKa = 5.71 DD38 pKa = 5.54 ANQFYY43 pKa = 10.76 LRR45 pKa = 11.84 YY46 pKa = 9.68 ARR48 pKa = 11.84 PFDD51 pKa = 3.85 ALGGNWLMRR60 pKa = 11.84 ATLPVNSFPDD70 pKa = 3.82 GTGGTEE76 pKa = 3.79 TGLGDD81 pKa = 3.88 FNIFAAYY88 pKa = 10.41 LFDD91 pKa = 3.77 TGNPAVSFGFGPQLTLPTASDD112 pKa = 3.72 DD113 pKa = 4.04 ALGSEE118 pKa = 4.26 KK119 pKa = 10.15 WSLGFANVLFNASSPVFQWGYY140 pKa = 10.35 LLTWQASIAGEE151 pKa = 4.11 DD152 pKa = 3.54 DD153 pKa = 3.67 RR154 pKa = 11.84 ADD156 pKa = 3.56 VNLGAFQPFGFYY168 pKa = 10.15 QLGAGWYY175 pKa = 9.01 LRR177 pKa = 11.84 SAAVWTYY184 pKa = 11.53 DD185 pKa = 3.95 FEE187 pKa = 4.93 TDD189 pKa = 3.9 AYY191 pKa = 10.24 AIPIGLGAGKK201 pKa = 10.24 VIRR204 pKa = 11.84 LEE206 pKa = 3.89 NAVMNVFVEE215 pKa = 4.16 PQYY218 pKa = 10.44 TVAHH222 pKa = 6.53 KK223 pKa = 10.85 GVGQPEE229 pKa = 4.04 WGVFGGVNFQFF240 pKa = 4.28
Molecular weight: 25.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.706
EMBOSS 3.834
Sillero 3.999
Patrickios 1.926
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A5B8G2S4|A0A5B8G2S4_9RHOB CCA tRNA nucleotidyltransferase OS=Paraoceanicella profunda OX=2579971 GN=FDP22_09555 PE=3 SV=1
MM1 pKa = 7.49 LRR3 pKa = 11.84 PGNRR7 pKa = 11.84 AGHH10 pKa = 5.83 TPASRR15 pKa = 11.84 AGQPPPAWRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 SRR29 pKa = 11.84 ARR31 pKa = 11.84 SSPPPPPSRR40 pKa = 11.84 GRR42 pKa = 11.84 RR43 pKa = 11.84 AAPRR47 pKa = 11.84 FGQDD51 pKa = 2.17 RR52 pKa = 11.84 RR53 pKa = 11.84 GRR55 pKa = 11.84 IRR57 pKa = 11.84 LASQASLTPRR67 pKa = 11.84 RR68 pKa = 11.84 WTRR71 pKa = 11.84 QAIRR75 pKa = 11.84 EE76 pKa = 4.16 ARR78 pKa = 11.84 RR79 pKa = 11.84 AA80 pKa = 3.42
Molecular weight: 9.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.476
IPC2_protein 11.052
IPC_protein 12.661
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.822
Wikipedia 13.305
Rodwell 12.325
Grimsley 12.866
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4636
0
4636
1511717
31
2704
326.1
35.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.839 ± 0.067
0.911 ± 0.011
5.531 ± 0.032
5.83 ± 0.03
3.528 ± 0.023
9.264 ± 0.043
2.009 ± 0.017
4.567 ± 0.028
2.19 ± 0.03
10.5 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.588 ± 0.019
2.068 ± 0.018
5.717 ± 0.034
2.694 ± 0.02
7.783 ± 0.036
5.029 ± 0.025
5.299 ± 0.022
7.26 ± 0.03
1.39 ± 0.014
2.001 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here