Clostridium sartagoforme

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2DIW2|A0A4S2DIW2_9CLOT PTS sugar transporter subunit IIA OS=Clostridium sartagoforme OX=84031 GN=E5347_10775 PE=4 SV=1
MM1 pKa = 7.6NEE3 pKa = 4.11EE4 pKa = 3.98IEE6 pKa = 4.42EE7 pKa = 4.06VLDD10 pKa = 3.62IYY12 pKa = 10.09EE13 pKa = 4.14TLIEE17 pKa = 4.34NGVTFYY23 pKa = 10.73YY24 pKa = 10.46GSEE27 pKa = 4.07IIPIGEE33 pKa = 4.07VTSFDD38 pKa = 3.41IFSDD42 pKa = 3.33EE43 pKa = 3.99MLEE46 pKa = 4.37IEE48 pKa = 4.7LDD50 pKa = 3.64GFNTYY55 pKa = 8.84EE56 pKa = 3.87VSIDD60 pKa = 3.83DD61 pKa = 4.27FIEE64 pKa = 4.03YY65 pKa = 9.84HH66 pKa = 6.25SKK68 pKa = 10.84EE69 pKa = 3.86GANYY73 pKa = 7.49HH74 pKa = 5.45TWPDD78 pKa = 2.7IRR80 pKa = 11.84KK81 pKa = 8.67FDD83 pKa = 3.78KK84 pKa = 10.98KK85 pKa = 10.67LGEE88 pKa = 4.13LFGMYY93 pKa = 9.88NN94 pKa = 3.16

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3RKV4|A0A4V3RKV4_9CLOT Tagatose-6-phosphate kinase OS=Clostridium sartagoforme OX=84031 GN=pfkB PE=3 SV=1
MM1 pKa = 7.46FMTYY5 pKa = 8.42QPKK8 pKa = 9.63KK9 pKa = 7.83RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.74RR21 pKa = 11.84MKK23 pKa = 9.03TSAGRR28 pKa = 11.84NVLKK32 pKa = 10.52RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3231

0

3231

1046381

29

2893

323.9

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.598 ± 0.045

0.947 ± 0.015

5.701 ± 0.038

7.794 ± 0.046

4.409 ± 0.031

6.5 ± 0.041

1.206 ± 0.015

10.134 ± 0.057

8.788 ± 0.042

9.209 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.018

6.604 ± 0.044

2.665 ± 0.024

1.933 ± 0.018

3.267 ± 0.026

6.533 ± 0.033

4.838 ± 0.033

6.563 ± 0.034

0.655 ± 0.014

4.203 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski