Streptomyces sp. MZ03-48

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553ZQS1|A0A553ZQS1_9ACTN Sec-independent protein translocase protein TatA OS=Streptomyces sp. MZ03-48 OX=2593677 GN=tatA PE=3 SV=1
MM1 pKa = 7.66GLRR4 pKa = 11.84TPAIPLSAGRR14 pKa = 11.84GCTSVQPASRR24 pKa = 11.84QPIQIRR30 pKa = 11.84QTGDD34 pKa = 3.01LEE36 pKa = 4.11GRR38 pKa = 11.84IMAKK42 pKa = 10.17AFQPSWRR49 pKa = 11.84QLVGLPWGGSHH60 pKa = 6.98RR61 pKa = 11.84PLAVVSSDD69 pKa = 3.07PTVTVGTTPTGVAITPDD86 pKa = 3.35GLHH89 pKa = 6.86AYY91 pKa = 8.44VANEE95 pKa = 3.65GSNDD99 pKa = 3.17VSVIDD104 pKa = 3.92TTSLTVTATVPVGTTPFWVAITPDD128 pKa = 3.36GLRR131 pKa = 11.84AYY133 pKa = 7.79VTNASDD139 pKa = 3.7GTVSVIDD146 pKa = 3.76TVTNTVTATIPVGTTPLGVAIGGTSAYY173 pKa = 8.02VTNASDD179 pKa = 3.7GTVSVIDD186 pKa = 3.76TVTNTVTATIPVGTAPFGVAIGGTSAYY213 pKa = 8.15VTNNGDD219 pKa = 3.48GTVSVIDD226 pKa = 3.6TGTNTVTATVTVGTAPLGVAVAGTHH251 pKa = 7.05AYY253 pKa = 8.99VANNGDD259 pKa = 3.68GTVSVIDD266 pKa = 3.6TGTNTVTATVTVGTAPLGVAVAGTHH291 pKa = 7.05AYY293 pKa = 8.99VANNGDD299 pKa = 3.68GTVSVIDD306 pKa = 3.6TGTNTVTATVTVGTAPLGVAVAGTHH331 pKa = 7.05AYY333 pKa = 8.99VANNGDD339 pKa = 3.68GTVSVIDD346 pKa = 3.6TGTNTVTATVTVGTAPLGVAVAGTHH371 pKa = 7.05AYY373 pKa = 8.99VANNGDD379 pKa = 3.79DD380 pKa = 3.78TVSVIDD386 pKa = 3.71TGTNTVTFTIPVGPGPFGVAITPDD410 pKa = 3.03GSTIYY415 pKa = 10.88VADD418 pKa = 3.77NADD421 pKa = 3.39NSVTVTAAVPFPTTTVLTSAPDD443 pKa = 3.58PSVFGEE449 pKa = 4.2AKK451 pKa = 9.75TLTATVTSSSGTPTGTVGFFDD472 pKa = 4.43GATLLGTGTLSGGVATFTTSALAVGSHH499 pKa = 6.87ALTAVYY505 pKa = 10.34GGDD508 pKa = 3.46TNFAGSTSPVDD519 pKa = 3.62TQTVGQAVTSTALSSAPDD537 pKa = 3.57PSVFGEE543 pKa = 4.2AKK545 pKa = 9.75TLTATVTVVAPGAGTPTGTVSFFDD569 pKa = 3.97GATLLGTGTLSGGVASFTTSSLSVGSHH596 pKa = 6.78ALTAVYY602 pKa = 10.57SGDD605 pKa = 3.35TDD607 pKa = 4.03FAGSTSPVDD616 pKa = 3.62TQTVGQAVTTTALSSAPDD634 pKa = 3.57PSVFGEE640 pKa = 4.2AKK642 pKa = 9.75TLTATVTVVAPGAGTLTGTVSFFDD666 pKa = 3.97GATLLGTGTLSGGVASFTTSSLSVGSHH693 pKa = 6.78ALTAVYY699 pKa = 10.47SGDD702 pKa = 3.85SNFTGSTSPVDD713 pKa = 3.63TQTVNQAATTTALTSAPDD731 pKa = 3.62PSVFGEE737 pKa = 4.2AKK739 pKa = 9.75TLTATVTVVAPGAGTPTGTVSFFDD763 pKa = 3.97GATLLGTGTLSGGVASFTTSALAVGSHH790 pKa = 6.87ALTAVYY796 pKa = 10.34GGDD799 pKa = 3.46TNFAGSTSPVDD810 pKa = 3.62TQTVGQAVTTTALSSAPDD828 pKa = 3.57PSVFGEE834 pKa = 4.2AKK836 pKa = 9.75TLTATVTVLAPGVGTPTGTVSFFDD860 pKa = 3.97GATLLGTGTLSGGVASFTTSTLGIGSHH887 pKa = 6.79ALTAVYY893 pKa = 10.43SGDD896 pKa = 3.64TNFVGSTSPVDD907 pKa = 3.63TQTVGQAATTTALSSAPDD925 pKa = 3.57PSVFGEE931 pKa = 4.2AKK933 pKa = 9.66TLTATVSAVSPGSGTPTGTVSFFDD957 pKa = 3.97GATLLGTGTLSGGVASFTTSSLSVGSHH984 pKa = 6.78ALTAVYY990 pKa = 10.57SGDD993 pKa = 3.35TDD995 pKa = 4.03FAGSTSPVDD1004 pKa = 3.58TQTVNQAATTTVLSSAPDD1022 pKa = 3.54PSVFGEE1028 pKa = 4.2AKK1030 pKa = 9.75TLTATVTVVAPGAGTLTGTVSFFDD1054 pKa = 3.97GATLLGTGTLSGGVASFTTSSLSVGSHH1081 pKa = 6.78ALTAVYY1087 pKa = 10.47SGDD1090 pKa = 3.85SNFTGSTSPVDD1101 pKa = 3.68TQTVGQAATSTALSSAPDD1119 pKa = 3.57PSVFGEE1125 pKa = 4.2AKK1127 pKa = 9.75TLTATVTVVAPGVGTPTGTVGFFDD1151 pKa = 4.43GATLLGTGTLSGGVASFTTSSLSVGSHH1178 pKa = 6.78ALTAVYY1184 pKa = 10.58SGDD1187 pKa = 3.41TDD1189 pKa = 3.42IAGSTSPVDD1198 pKa = 3.62TQTVGQAATSTALSSAPDD1216 pKa = 3.57PSVFGEE1222 pKa = 4.2AKK1224 pKa = 9.75TLTATVTVVAPGVGTPTGTVGFFDD1248 pKa = 4.43GATLLGTGTLSGGVATFTTSTLGIGSHH1275 pKa = 6.79ALTAVYY1281 pKa = 10.43SGDD1284 pKa = 3.64TNFVGSTSPVDD1295 pKa = 3.61TQTVNQAATSTALSSAPDD1313 pKa = 3.57PSVFGEE1319 pKa = 4.2AKK1321 pKa = 9.75TLTATVTVVAPGVGTPTGTVSFFDD1345 pKa = 3.97GATLLGTGTLSGGVASFTTSSLSVGSHH1372 pKa = 6.78ALTAVYY1378 pKa = 10.36SGDD1381 pKa = 3.15IDD1383 pKa = 4.21FVGSTSPVDD1392 pKa = 3.63TQTVGQAATTTALTSAPDD1410 pKa = 3.69PSVFGQAKK1418 pKa = 8.14TLTATVSAVAPGAGTPTGTVSFFDD1442 pKa = 3.97GATLLGTGTLSGGVATFTTSSLSVGSHH1469 pKa = 6.78ALTAVYY1475 pKa = 10.47SGDD1478 pKa = 3.85SNFTGSTSPVDD1489 pKa = 3.63TQTVNQAATTTALSSAPDD1507 pKa = 3.68PSVFGQAKK1515 pKa = 8.22TLTATVTAVPPGSGTPTGTVSFFDD1539 pKa = 3.97GATLLGTGTLSGGVASFTTSTLGIGTHH1566 pKa = 5.96SLTAVYY1572 pKa = 10.32NGSGNFNTSTSPVDD1586 pKa = 3.51TQTVTKK1592 pKa = 10.66NLTTTALTSAPDD1604 pKa = 3.69PSVFGQAKK1612 pKa = 8.22TLTATVTAVPPGSGTPTGTVSFFDD1636 pKa = 3.99GATLLGG1642 pKa = 3.94

Molecular weight: 157.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553YVP9|A0A553YVP9_9ACTN Maltose alpha-D-glucosyltransferase OS=Streptomyces sp. MZ03-48 OX=2593677 GN=treS PE=3 SV=1
MM1 pKa = 7.25GHH3 pKa = 7.23LLLRR7 pKa = 11.84GLLRR11 pKa = 11.84TLLLRR16 pKa = 11.84LRR18 pKa = 11.84HH19 pKa = 5.61LRR21 pKa = 11.84GLLRR25 pKa = 11.84LLGLGLLLRR34 pKa = 11.84GRR36 pKa = 11.84RR37 pKa = 11.84LLLRR41 pKa = 11.84RR42 pKa = 11.84LLLRR46 pKa = 11.84LLGVRR51 pKa = 11.84VLLLNLRR58 pKa = 11.84GRR60 pKa = 11.84LLLVRR65 pKa = 11.84AGLLLRR71 pKa = 11.84LRR73 pKa = 11.84RR74 pKa = 11.84LRR76 pKa = 11.84VRR78 pKa = 11.84ARR80 pKa = 11.84LLGLLDD86 pKa = 3.42VRR88 pKa = 11.84VLLGRR93 pKa = 11.84GLLGMRR99 pKa = 11.84ARR101 pKa = 11.84LLRR104 pKa = 4.4

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5822

0

5822

1838379

24

12400

315.8

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.046 ± 0.054

0.827 ± 0.012

5.959 ± 0.028

5.589 ± 0.037

2.675 ± 0.019

9.691 ± 0.037

2.441 ± 0.018

3.07 ± 0.023

2.199 ± 0.029

10.335 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.718 ± 0.011

1.68 ± 0.018

6.288 ± 0.032

2.718 ± 0.034

8.241 ± 0.039

4.765 ± 0.028

6.168 ± 0.029

8.202 ± 0.036

1.419 ± 0.014

1.97 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski