Amino acid dipepetide frequency for Streptomyces sp. MZ03-48

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.282AlaAla: 23.282 ± 0.213	1.118AlaCys: 1.118 ± 0.031	8.647AlaAsp: 8.647 ± 0.084	8.693AlaGlu: 8.693 ± 0.089	3.555AlaPhe: 3.555 ± 0.051	13.091AlaGly: 13.091 ± 0.114	3.288AlaHis: 3.288 ± 0.047	3.126AlaIle: 3.126 ± 0.05	2.957AlaLys: 2.957 ± 0.053	15.269AlaLeu: 15.269 ± 0.125	2.504AlaMet: 2.504 ± 0.041	1.819AlaAsn: 1.819 ± 0.033	8.206AlaPro: 8.206 ± 0.089	3.769AlaGln: 3.769 ± 0.052	11.036AlaArg: 11.036 ± 0.094	5.649AlaSer: 5.649 ± 0.061	7.583AlaThr: 7.583 ± 0.068	12.315AlaVal: 12.315 ± 0.122	1.804AlaTrp: 1.804 ± 0.034	2.748AlaTyr: 2.748 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.273CysAla: 1.273 ± 0.031	0.107CysCys: 0.107 ± 0.008	0.481CysAsp: 0.481 ± 0.019	0.404CysGlu: 0.404 ± 0.015	0.233CysPhe: 0.233 ± 0.011	1.026CysGly: 1.026 ± 0.027	0.209CysHis: 0.209 ± 0.01	0.185CysIle: 0.185 ± 0.01	0.106CysLys: 0.106 ± 0.009	0.735CysLeu: 0.735 ± 0.021	0.122CysMet: 0.122 ± 0.009	0.127CysAsn: 0.127 ± 0.009	0.496CysPro: 0.496 ± 0.018	0.177CysGln: 0.177 ± 0.01	0.681CysArg: 0.681 ± 0.022	0.395CysSer: 0.395 ± 0.015	0.57CysThr: 0.57 ± 0.02	0.671CysVal: 0.671 ± 0.022	0.118CysTrp: 0.118 ± 0.008	0.152CysTyr: 0.152 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.652AspAla: 8.652 ± 0.087	0.424AspCys: 0.424 ± 0.019	3.611AspAsp: 3.611 ± 0.048	3.726AspGlu: 3.726 ± 0.048	1.523AspPhe: 1.523 ± 0.031	6.894AspGly: 6.894 ± 0.079	1.558AspHis: 1.558 ± 0.032	1.851AspIle: 1.851 ± 0.034	1.137AspLys: 1.137 ± 0.033	6.08AspLeu: 6.08 ± 0.067	0.766AspMet: 0.766 ± 0.021	0.899AspAsn: 0.899 ± 0.027	4.372AspPro: 4.372 ± 0.055	1.388AspGln: 1.388 ± 0.034	5.031AspArg: 5.031 ± 0.059	2.249AspSer: 2.249 ± 0.04	3.239AspThr: 3.239 ± 0.049	4.329AspVal: 4.329 ± 0.046	0.912AspTrp: 0.912 ± 0.022	0.945AspTyr: 0.945 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.384GluAla: 7.384 ± 0.088	0.369GluCys: 0.369 ± 0.014	2.668GluAsp: 2.668 ± 0.045	3.665GluGlu: 3.665 ± 0.073	1.432GluPhe: 1.432 ± 0.028	4.295GluGly: 4.295 ± 0.05	1.399GluHis: 1.399 ± 0.034	2.259GluIle: 2.259 ± 0.039	1.496GluLys: 1.496 ± 0.038	6.562GluLeu: 6.562 ± 0.084	0.941GluMet: 0.941 ± 0.025	1.003GluAsn: 1.003 ± 0.028	3.239GluPro: 3.239 ± 0.041	2.168GluGln: 2.168 ± 0.053	5.582GluArg: 5.582 ± 0.074	2.397GluSer: 2.397 ± 0.041	2.83GluThr: 2.83 ± 0.039	4.479GluVal: 4.479 ± 0.057	0.729GluTrp: 0.729 ± 0.02	0.992GluTyr: 0.992 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.756PheAla: 3.756 ± 0.052	0.289PheCys: 0.289 ± 0.012	1.853PheAsp: 1.853 ± 0.032	1.266PheGlu: 1.266 ± 0.03	0.875PhePhe: 0.875 ± 0.025	3.044PheGly: 3.044 ± 0.043	0.673PheHis: 0.673 ± 0.021	0.701PheIle: 0.701 ± 0.019	0.483PheLys: 0.483 ± 0.019	2.627PheLeu: 2.627 ± 0.037	0.367PheMet: 0.367 ± 0.016	0.509PheAsn: 0.509 ± 0.017	1.396PhePro: 1.396 ± 0.027	0.642PheGln: 0.642 ± 0.018	1.882PheArg: 1.882 ± 0.034	1.39PheSer: 1.39 ± 0.026	2.019PheThr: 2.019 ± 0.04	2.077PheVal: 2.077 ± 0.03	0.393PheTrp: 0.393 ± 0.014	0.507PheTyr: 0.507 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.284GlyAla: 12.284 ± 0.091	0.935GlyCys: 0.935 ± 0.027	5.324GlyAsp: 5.324 ± 0.06	5.161GlyGlu: 5.161 ± 0.06	2.826GlyPhe: 2.826 ± 0.051	9.449GlyGly: 9.449 ± 0.113	2.44GlyHis: 2.44 ± 0.04	3.436GlyIle: 3.436 ± 0.049	2.6GlyLys: 2.6 ± 0.049	9.399GlyLeu: 9.399 ± 0.084	2.09GlyMet: 2.09 ± 0.036	1.76GlyAsn: 1.76 ± 0.04	5.745GlyPro: 5.745 ± 0.066	2.612GlyGln: 2.612 ± 0.048	7.94GlyArg: 7.94 ± 0.08	5.161GlySer: 5.161 ± 0.054	6.462GlyThr: 6.462 ± 0.073	7.488GlyVal: 7.488 ± 0.071	1.622GlyTrp: 1.622 ± 0.031	2.174GlyTyr: 2.174 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.931HisAla: 2.931 ± 0.044	0.217HisCys: 0.217 ± 0.011	1.446HisAsp: 1.446 ± 0.029	1.152HisGlu: 1.152 ± 0.027	0.707HisPhe: 0.707 ± 0.021	2.671HisGly: 2.671 ± 0.044	0.826HisHis: 0.826 ± 0.024	0.688HisIle: 0.688 ± 0.019	0.402HisLys: 0.402 ± 0.015	2.681HisLeu: 2.681 ± 0.042	0.344HisMet: 0.344 ± 0.014	0.358HisAsn: 0.358 ± 0.013	1.98HisPro: 1.98 ± 0.031	0.672HisGln: 0.672 ± 0.022	2.367HisArg: 2.367 ± 0.042	1.022HisSer: 1.022 ± 0.028	1.527HisThr: 1.527 ± 0.033	1.599HisVal: 1.599 ± 0.034	0.367HisTrp: 0.367 ± 0.015	0.449HisTyr: 0.449 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.605IleAla: 4.605 ± 0.056	0.32IleCys: 0.32 ± 0.014	2.216IleAsp: 2.216 ± 0.037	1.917IleGlu: 1.917 ± 0.036	0.684IlePhe: 0.684 ± 0.023	3.653IleGly: 3.653 ± 0.057	0.62IleHis: 0.62 ± 0.019	0.846IleIle: 0.846 ± 0.026	0.776IleLys: 0.776 ± 0.023	2.247IleLeu: 2.247 ± 0.042	0.416IleMet: 0.416 ± 0.015	0.682IleAsn: 0.682 ± 0.02	1.767IlePro: 1.767 ± 0.038	0.678IleGln: 0.678 ± 0.022	2.258IleArg: 2.258 ± 0.037	1.562IleSer: 1.562 ± 0.031	2.015IleThr: 2.015 ± 0.037	2.621IleVal: 2.621 ± 0.043	0.348IleTrp: 0.348 ± 0.014	0.474IleTyr: 0.474 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.864LysAla: 2.864 ± 0.05	0.111LysCys: 0.111 ± 0.008	1.421LysAsp: 1.421 ± 0.036	1.306LysGlu: 1.306 ± 0.035	0.466LysPhe: 0.466 ± 0.017	1.982LysGly: 1.982 ± 0.046	0.416LysHis: 0.416 ± 0.013	0.936LysIle: 0.936 ± 0.024	0.998LysLys: 0.998 ± 0.039	2.043LysLeu: 2.043 ± 0.038	0.395LysMet: 0.395 ± 0.016	0.542LysAsn: 0.542 ± 0.018	1.329LysPro: 1.329 ± 0.035	0.696LysGln: 0.696 ± 0.026	1.463LysArg: 1.463 ± 0.033	1.153LysSer: 1.153 ± 0.031	1.301LysThr: 1.301 ± 0.034	1.888LysVal: 1.888 ± 0.041	0.238LysTrp: 0.238 ± 0.011	0.437LysTyr: 0.437 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.261LeuAla: 15.261 ± 0.125	0.867LeuCys: 0.867 ± 0.027	6.662LeuAsp: 6.662 ± 0.07	4.514LeuGlu: 4.514 ± 0.069	2.6LeuPhe: 2.6 ± 0.04	9.285LeuGly: 9.285 ± 0.082	2.523LeuHis: 2.523 ± 0.045	3.332LeuIle: 3.332 ± 0.048	2.036LeuLys: 2.036 ± 0.045	11.317LeuLeu: 11.317 ± 0.12	1.595LeuMet: 1.595 ± 0.033	1.62LeuAsn: 1.62 ± 0.033	6.748LeuPro: 6.748 ± 0.067	2.17LeuGln: 2.17 ± 0.036	8.995LeuArg: 8.995 ± 0.082	5.202LeuSer: 5.202 ± 0.06	7.309LeuThr: 7.309 ± 0.068	8.342LeuVal: 8.342 ± 0.087	1.214LeuTrp: 1.214 ± 0.027	1.761LeuTyr: 1.761 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.313MetAla: 2.313 ± 0.039	0.137MetCys: 0.137 ± 0.009	0.914MetAsp: 0.914 ± 0.021	0.735MetGlu: 0.735 ± 0.019	0.419MetPhe: 0.419 ± 0.017	1.329MetGly: 1.329 ± 0.033	0.364MetHis: 0.364 ± 0.014	0.636MetIle: 0.636 ± 0.021	0.416MetLys: 0.416 ± 0.015	1.68MetLeu: 1.68 ± 0.032	0.299MetMet: 0.299 ± 0.013	0.416MetAsn: 0.416 ± 0.015	1.09MetPro: 1.09 ± 0.026	0.427MetGln: 0.427 ± 0.017	1.415MetArg: 1.415 ± 0.028	1.287MetSer: 1.287 ± 0.025	1.505MetThr: 1.505 ± 0.032	1.294MetVal: 1.294 ± 0.03	0.202MetTrp: 0.202 ± 0.011	0.305MetTyr: 0.305 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.151AsnAla: 2.151 ± 0.041	0.162AsnCys: 0.162 ± 0.01	0.941AsnAsp: 0.941 ± 0.022	0.79AsnGlu: 0.79 ± 0.023	0.446AsnPhe: 0.446 ± 0.017	1.82AsnGly: 1.82 ± 0.037	0.412AsnHis: 0.412 ± 0.015	0.613AsnIle: 0.613 ± 0.02	0.423AsnLys: 0.423 ± 0.015	1.56AsnLeu: 1.56 ± 0.033	0.286AsnMet: 0.286 ± 0.012	0.401AsnAsn: 0.401 ± 0.015	1.265AsnPro: 1.265 ± 0.024	0.451AsnGln: 0.451 ± 0.017	1.212AsnArg: 1.212 ± 0.025	0.877AsnSer: 0.877 ± 0.027	1.079AsnThr: 1.079 ± 0.028	1.292AsnVal: 1.292 ± 0.032	0.262AsnTrp: 0.262 ± 0.012	0.358AsnTyr: 0.358 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.802ProAla: 8.802 ± 0.075	0.404ProCys: 0.404 ± 0.015	4.662ProAsp: 4.662 ± 0.051	4.476ProGlu: 4.476 ± 0.053	1.529ProPhe: 1.529 ± 0.029	7.452ProGly: 7.452 ± 0.084	1.591ProHis: 1.591 ± 0.036	1.162ProIle: 1.162 ± 0.029	1.202ProLys: 1.202 ± 0.036	5.587ProLeu: 5.587 ± 0.063	0.975ProMet: 0.975 ± 0.023	0.832ProAsn: 0.832 ± 0.025	3.782ProPro: 3.782 ± 0.074	2.019ProGln: 2.019 ± 0.059	4.25ProArg: 4.25 ± 0.051	3.136ProSer: 3.136 ± 0.05	3.112ProThr: 3.112 ± 0.048	5.578ProVal: 5.578 ± 0.055	0.884ProTrp: 0.884 ± 0.022	1.441ProTyr: 1.441 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.563GlnAla: 3.563 ± 0.054	0.167GlnCys: 0.167 ± 0.009	1.399GlnAsp: 1.399 ± 0.033	1.607GlnGlu: 1.607 ± 0.043	0.657GlnPhe: 0.657 ± 0.018	2.254GlnGly: 2.254 ± 0.039	0.674GlnHis: 0.674 ± 0.027	1.113GlnIle: 1.113 ± 0.027	0.613GlnLys: 0.613 ± 0.021	3.186GlnLeu: 3.186 ± 0.05	0.498GlnMet: 0.498 ± 0.017	0.468GlnAsn: 0.468 ± 0.018	1.67GlnPro: 1.67 ± 0.045	1.302GlnGln: 1.302 ± 0.053	2.396GlnArg: 2.396 ± 0.043	1.184GlnSer: 1.184 ± 0.034	1.257GlnThr: 1.257 ± 0.032	2.218GlnVal: 2.218 ± 0.042	0.435GlnTrp: 0.435 ± 0.017	0.516GlnTyr: 0.516 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	10.546ArgAla: 10.546 ± 0.107	0.656ArgCys: 0.656 ± 0.022	4.372ArgAsp: 4.372 ± 0.053	4.754ArgGlu: 4.754 ± 0.061	2.263ArgPhe: 2.263 ± 0.039	6.185ArgGly: 6.185 ± 0.07	2.25ArgHis: 2.25 ± 0.039	3.271ArgIle: 3.271 ± 0.039	1.729ArgLys: 1.729 ± 0.035	8.828ArgLeu: 8.828 ± 0.098	1.777ArgMet: 1.777 ± 0.033	1.402ArgAsn: 1.402 ± 0.031	5.369ArgPro: 5.369 ± 0.059	2.301ArgGln: 2.301 ± 0.04	8.276ArgArg: 8.276 ± 0.088	3.98ArgSer: 3.98 ± 0.048	5.666ArgThr: 5.666 ± 0.065	5.536ArgVal: 5.536 ± 0.054	1.376ArgTrp: 1.376 ± 0.028	1.878ArgTyr: 1.878 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.33SerAla: 6.33 ± 0.06	0.43SerCys: 0.43 ± 0.017	2.505SerAsp: 2.505 ± 0.042	2.242SerGlu: 2.242 ± 0.042	1.496SerPhe: 1.496 ± 0.029	6.091SerGly: 6.091 ± 0.074	1.055SerHis: 1.055 ± 0.027	1.221SerIle: 1.221 ± 0.028	0.987SerLys: 0.987 ± 0.029	4.582SerLeu: 4.582 ± 0.06	0.992SerMet: 0.992 ± 0.026	0.785SerAsn: 0.785 ± 0.023	3.013SerPro: 3.013 ± 0.041	1.202SerGln: 1.202 ± 0.028	3.531SerArg: 3.531 ± 0.048	2.527SerSer: 2.527 ± 0.052	2.716SerThr: 2.716 ± 0.05	3.996SerVal: 3.996 ± 0.049	0.832SerTrp: 0.832 ± 0.025	1.119SerTyr: 1.119 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.358ThrAla: 9.358 ± 0.078	0.432ThrCys: 0.432 ± 0.017	3.706ThrAsp: 3.706 ± 0.045	3.224ThrGlu: 3.224 ± 0.051	1.676ThrPhe: 1.676 ± 0.034	6.878ThrGly: 6.878 ± 0.068	1.294ThrHis: 1.294 ± 0.03	1.498ThrIle: 1.498 ± 0.028	1.197ThrLys: 1.197 ± 0.031	5.936ThrLeu: 5.936 ± 0.058	0.959ThrMet: 0.959 ± 0.025	0.924ThrAsn: 0.924 ± 0.025	4.254ThrPro: 4.254 ± 0.061	1.296ThrGln: 1.296 ± 0.038	3.819ThrArg: 3.819 ± 0.05	2.974ThrSer: 2.974 ± 0.047	3.832ThrThr: 3.832 ± 0.057	6.326ThrVal: 6.326 ± 0.074	0.814ThrTrp: 0.814 ± 0.026	1.284ThrTyr: 1.284 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.77ValAla: 10.77 ± 0.094	0.796ValCys: 0.796 ± 0.022	4.618ValAsp: 4.618 ± 0.054	4.432ValGlu: 4.432 ± 0.057	2.25ValPhe: 2.25 ± 0.037	6.306ValGly: 6.306 ± 0.072	2.033ValHis: 2.033 ± 0.033	2.885ValIle: 2.885 ± 0.045	1.696ValLys: 1.696 ± 0.037	9.214ValLeu: 9.214 ± 0.092	1.357ValMet: 1.357 ± 0.027	1.588ValAsn: 1.588 ± 0.033	5.398ValPro: 5.398 ± 0.057	2.037ValGln: 2.037 ± 0.03	7.166ValArg: 7.166 ± 0.068	3.876ValSer: 3.876 ± 0.054	5.653ValThr: 5.653 ± 0.067	7.467ValVal: 7.467 ± 0.081	1.02ValTrp: 1.02 ± 0.023	1.456ValTyr: 1.456 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.562TrpAla: 1.562 ± 0.034	0.166TrpCys: 0.166 ± 0.009	0.75TrpAsp: 0.75 ± 0.021	0.731TrpGlu: 0.731 ± 0.023	0.494TrpPhe: 0.494 ± 0.018	1.0TrpGly: 1.0 ± 0.024	0.36TrpHis: 0.36 ± 0.012	0.518TrpIle: 0.518 ± 0.016	0.36TrpLys: 0.36 ± 0.014	1.716TrpLeu: 1.716 ± 0.033	0.274TrpMet: 0.274 ± 0.01	0.322TrpAsn: 0.322 ± 0.013	0.764TrpPro: 0.764 ± 0.021	0.617TrpGln: 0.617 ± 0.018	1.298TrpArg: 1.298 ± 0.026	0.814TrpSer: 0.814 ± 0.023	0.907TrpThr: 0.907 ± 0.023	0.911TrpVal: 0.911 ± 0.023	0.306TrpTrp: 0.306 ± 0.014	0.315TrpTyr: 0.315 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.771TyrAla: 2.771 ± 0.042	0.162TyrCys: 0.162 ± 0.008	1.39TyrAsp: 1.39 ± 0.032	1.095TyrGlu: 1.095 ± 0.024	0.621TyrPhe: 0.621 ± 0.019	2.201TyrGly: 2.201 ± 0.04	0.422TyrHis: 0.422 ± 0.016	0.426TyrIle: 0.426 ± 0.017	0.372TyrLys: 0.372 ± 0.016	2.105TyrLeu: 2.105 ± 0.032	0.224TyrMet: 0.224 ± 0.012	0.344TyrAsn: 0.344 ± 0.015	0.993TyrPro: 0.993 ± 0.028	0.552TyrGln: 0.552 ± 0.019	1.815TyrArg: 1.815 ± 0.027	0.815TyrSer: 0.815 ± 0.024	1.102TyrThr: 1.102 ± 0.028	1.591TyrVal: 1.591 ± 0.035	0.309TyrTrp: 0.309 ± 0.014	0.394TyrTyr: 0.394 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5822 proteins (1838379 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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