Caldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor; Caldicellulosiruptor owensensis

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4Q1Q0|E4Q1Q0_CALOW Phosphoribosylformylglycinamidine cyclo-ligase OS=Caldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL) OX=632518 GN=purM PE=3 SV=1
MM1 pKa = 7.68EE2 pKa = 4.66IWICSICGYY11 pKa = 10.25EE12 pKa = 3.87YY13 pKa = 10.99NPEE16 pKa = 4.16NGDD19 pKa = 3.63PEE21 pKa = 4.67NGIEE25 pKa = 4.31PGTKK29 pKa = 9.91FEE31 pKa = 5.44DD32 pKa = 4.81LPDD35 pKa = 3.81DD36 pKa = 4.12WVCPVCGVGKK46 pKa = 10.56DD47 pKa = 3.54MFEE50 pKa = 4.32KK51 pKa = 10.67KK52 pKa = 10.2

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4Q1D5|E4Q1D5_CALOW Agmatinase OS=Caldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL) OX=632518 GN=Calow_1106 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.05QPHH8 pKa = 5.65NKK10 pKa = 8.82RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.42RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.66GGRR28 pKa = 11.84KK29 pKa = 6.88VLKK32 pKa = 10.0RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2133

0

2133

692621

33

1790

324.7

36.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.94 ± 0.054

1.119 ± 0.02

5.193 ± 0.037

7.677 ± 0.057

5.002 ± 0.045

6.088 ± 0.05

1.362 ± 0.02

9.408 ± 0.043

9.029 ± 0.052

9.294 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.023

4.936 ± 0.036

3.232 ± 0.032

2.907 ± 0.03

4.012 ± 0.037

5.863 ± 0.044

4.506 ± 0.038

7.211 ± 0.045

0.823 ± 0.018

4.153 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski