Hydrogenophaga sp. Root209

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T2ZFM1|A0A0T2ZFM1_9BURK Enoyl-CoA hydratase OS=Hydrogenophaga sp. Root209 OX=1736490 GN=ASE11_22095 PE=3 SV=1
MM1 pKa = 8.24PDD3 pKa = 3.45TPEE6 pKa = 4.0KK7 pKa = 7.56TTPAALPPPGEE18 pKa = 4.01ATLPCVMVFNANDD31 pKa = 3.66PSGAGGLSADD41 pKa = 3.4ITAMSSASVHH51 pKa = 5.32VLSVVTGAYY60 pKa = 10.13VRR62 pKa = 11.84DD63 pKa = 3.48TSDD66 pKa = 2.8IHH68 pKa = 7.7DD69 pKa = 3.91HH70 pKa = 6.28FAFDD74 pKa = 3.84EE75 pKa = 4.28EE76 pKa = 4.54AVADD80 pKa = 3.95QARR83 pKa = 11.84CALEE87 pKa = 4.41DD88 pKa = 3.59MPVQAFKK95 pKa = 11.28VGFVGNPEE103 pKa = 4.06NLSVVAEE110 pKa = 4.31ITADD114 pKa = 3.72YY115 pKa = 11.3ADD117 pKa = 4.15VPVIAYY123 pKa = 8.5MPDD126 pKa = 3.62LSWWDD131 pKa = 3.42EE132 pKa = 4.12LAIEE136 pKa = 4.58TYY138 pKa = 11.04LDD140 pKa = 3.47ASAEE144 pKa = 3.99LLLPQTTVLVGNHH157 pKa = 5.01STLCRR162 pKa = 11.84WLLPEE167 pKa = 4.31WEE169 pKa = 4.56GDD171 pKa = 3.62RR172 pKa = 11.84PPSPRR177 pKa = 11.84DD178 pKa = 3.24VARR181 pKa = 11.84AAAVHH186 pKa = 5.84GVPYY190 pKa = 10.28TLVTGFNAADD200 pKa = 3.5QYY202 pKa = 11.67LEE204 pKa = 4.05SHH206 pKa = 6.82LASPEE211 pKa = 3.89AVLATARR218 pKa = 11.84YY219 pKa = 8.65EE220 pKa = 3.99RR221 pKa = 11.84FEE223 pKa = 4.21ATFSGAGDD231 pKa = 3.74TLSAALCALIAGGSDD246 pKa = 3.37LQSACAEE253 pKa = 3.93ALTYY257 pKa = 10.72LDD259 pKa = 3.41QCLDD263 pKa = 3.39AGFQPGMGHH272 pKa = 7.25AVPDD276 pKa = 3.71RR277 pKa = 11.84LFWAHH282 pKa = 6.89EE283 pKa = 3.97EE284 pKa = 4.21DD285 pKa = 5.68EE286 pKa = 4.32EE287 pKa = 6.31DD288 pKa = 3.65EE289 pKa = 4.6DD290 pKa = 5.69ASAEE294 pKa = 4.05PASDD298 pKa = 3.57STLDD302 pKa = 4.15ADD304 pKa = 5.84DD305 pKa = 5.23FPLDD309 pKa = 3.9TTRR312 pKa = 11.84HH313 pKa = 4.6

Molecular weight: 33.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T2YY86|A0A0T2YY86_9BURK Methionine biosynthesis protein MetW OS=Hydrogenophaga sp. Root209 OX=1736490 GN=ASE11_24740 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4859

0

4859

1561390

24

3398

321.3

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.444 ± 0.05

0.925 ± 0.012

5.172 ± 0.025

5.226 ± 0.028

3.522 ± 0.019

8.348 ± 0.032

2.397 ± 0.018

4.256 ± 0.026

3.165 ± 0.036

10.903 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.017

2.639 ± 0.02

5.242 ± 0.026

4.038 ± 0.022

6.772 ± 0.033

5.462 ± 0.027

5.364 ± 0.025

7.921 ± 0.029

1.513 ± 0.014

2.049 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski