Amino acid dipepetide frequency for Hydrogenophaga sp. Root209

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.17AlaAla: 16.17 ± 0.15	1.309AlaCys: 1.309 ± 0.034	6.212AlaAsp: 6.212 ± 0.069	6.227AlaGlu: 6.227 ± 0.067	4.209AlaPhe: 4.209 ± 0.051	10.112AlaGly: 10.112 ± 0.098	2.833AlaHis: 2.833 ± 0.046	4.978AlaIle: 4.978 ± 0.068	3.506AlaLys: 3.506 ± 0.064	15.056AlaLeu: 15.056 ± 0.138	3.743AlaMet: 3.743 ± 0.054	2.822AlaAsn: 2.822 ± 0.044	6.013AlaPro: 6.013 ± 0.078	5.915AlaGln: 5.915 ± 0.075	8.635AlaArg: 8.635 ± 0.087	6.909AlaSer: 6.909 ± 0.072	6.178AlaThr: 6.178 ± 0.073	9.231AlaVal: 9.231 ± 0.082	2.013AlaTrp: 2.013 ± 0.035	2.366AlaTyr: 2.366 ± 0.042	0.001AlaXaa: 0.001 ± 0.001
Cys	1.208CysAla: 1.208 ± 0.031	0.109CysCys: 0.109 ± 0.009	0.533CysAsp: 0.533 ± 0.018	0.55CysGlu: 0.55 ± 0.018	0.305CysPhe: 0.305 ± 0.013	0.949CysGly: 0.949 ± 0.025	0.267CysHis: 0.267 ± 0.012	0.403CysIle: 0.403 ± 0.017	0.219CysLys: 0.219 ± 0.011	0.853CysLeu: 0.853 ± 0.022	0.2CysMet: 0.2 ± 0.013	0.239CysAsn: 0.239 ± 0.013	0.514CysPro: 0.514 ± 0.021	0.267CysGln: 0.267 ± 0.013	0.562CysArg: 0.562 ± 0.018	0.517CysSer: 0.517 ± 0.02	0.507CysThr: 0.507 ± 0.021	0.752CysVal: 0.752 ± 0.021	0.108CysTrp: 0.108 ± 0.008	0.19CysTyr: 0.19 ± 0.012	0.001CysXaa: 0.001 ± 0.001
Asp	6.83AspAla: 6.83 ± 0.081	0.439AspCys: 0.439 ± 0.018	2.665AspAsp: 2.665 ± 0.043	3.104AspGlu: 3.104 ± 0.049	2.017AspPhe: 2.017 ± 0.038	4.409AspGly: 4.409 ± 0.057	1.257AspHis: 1.257 ± 0.027	2.399AspIle: 2.399 ± 0.041	1.631AspLys: 1.631 ± 0.046	5.407AspLeu: 5.407 ± 0.056	1.319AspMet: 1.319 ± 0.03	1.214AspAsn: 1.214 ± 0.031	2.849AspPro: 2.849 ± 0.049	1.674AspGln: 1.674 ± 0.034	3.293AspArg: 3.293 ± 0.045	2.235AspSer: 2.235 ± 0.041	2.675AspThr: 2.675 ± 0.04	4.244AspVal: 4.244 ± 0.058	0.909AspTrp: 0.909 ± 0.025	1.153AspTyr: 1.153 ± 0.029	0.001AspXaa: 0.001 ± 0.001
Glu	6.695GluAla: 6.695 ± 0.067	0.407GluCys: 0.407 ± 0.018	2.178GluAsp: 2.178 ± 0.035	2.307GluGlu: 2.307 ± 0.043	1.736GluPhe: 1.736 ± 0.033	3.768GluGly: 3.768 ± 0.056	1.469GluHis: 1.469 ± 0.031	2.477GluIle: 2.477 ± 0.047	1.808GluLys: 1.808 ± 0.042	5.993GluLeu: 5.993 ± 0.068	1.334GluMet: 1.334 ± 0.035	1.208GluAsn: 1.208 ± 0.028	2.661GluPro: 2.661 ± 0.042	2.531GluGln: 2.531 ± 0.045	4.704GluArg: 4.704 ± 0.058	2.596GluSer: 2.596 ± 0.041	2.591GluThr: 2.591 ± 0.047	4.128GluVal: 4.128 ± 0.052	0.718GluTrp: 0.718 ± 0.021	0.949GluTyr: 0.949 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.089PheAla: 4.089 ± 0.055	0.399PheCys: 0.399 ± 0.016	2.484PheAsp: 2.484 ± 0.043	2.194PheGlu: 2.194 ± 0.042	1.377PhePhe: 1.377 ± 0.031	3.323PheGly: 3.323 ± 0.046	0.781PheHis: 0.781 ± 0.02	1.488PheIle: 1.488 ± 0.029	1.24PheLys: 1.24 ± 0.027	3.063PheLeu: 3.063 ± 0.05	0.9PheMet: 0.9 ± 0.024	1.187PheAsn: 1.187 ± 0.029	1.474PhePro: 1.474 ± 0.03	1.084PheGln: 1.084 ± 0.028	1.805PheArg: 1.805 ± 0.029	2.156PheSer: 2.156 ± 0.037	2.004PheThr: 2.004 ± 0.037	2.852PheVal: 2.852 ± 0.045	0.498PheTrp: 0.498 ± 0.02	0.818PheTyr: 0.818 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	9.05GlyAla: 9.05 ± 0.098	0.876GlyCys: 0.876 ± 0.022	3.879GlyAsp: 3.879 ± 0.055	4.439GlyGlu: 4.439 ± 0.059	3.417GlyPhe: 3.417 ± 0.039	6.989GlyGly: 6.989 ± 0.106	2.115GlyHis: 2.115 ± 0.039	3.888GlyIle: 3.888 ± 0.054	3.035GlyLys: 3.035 ± 0.061	9.402GlyLeu: 9.402 ± 0.092	2.534GlyMet: 2.534 ± 0.045	2.144GlyAsn: 2.144 ± 0.041	3.157GlyPro: 3.157 ± 0.053	3.494GlyGln: 3.494 ± 0.047	5.186GlyArg: 5.186 ± 0.058	4.7GlySer: 4.7 ± 0.054	4.486GlyThr: 4.486 ± 0.061	7.068GlyVal: 7.068 ± 0.07	1.49GlyTrp: 1.49 ± 0.033	2.132GlyTyr: 2.132 ± 0.04	0.001GlyXaa: 0.001 ± 0.001
His	2.993HisAla: 2.993 ± 0.047	0.284HisCys: 0.284 ± 0.015	1.199HisAsp: 1.199 ± 0.028	1.198HisGlu: 1.198 ± 0.028	0.914HisPhe: 0.914 ± 0.026	2.267HisGly: 2.267 ± 0.043	0.734HisHis: 0.734 ± 0.026	1.055HisIle: 1.055 ± 0.028	0.63HisLys: 0.63 ± 0.022	2.527HisLeu: 2.527 ± 0.044	0.539HisMet: 0.539 ± 0.015	0.569HisAsn: 0.569 ± 0.021	1.555HisPro: 1.555 ± 0.033	0.851HisGln: 0.851 ± 0.025	1.581HisArg: 1.581 ± 0.037	1.125HisSer: 1.125 ± 0.027	1.237HisThr: 1.237 ± 0.029	1.722HisVal: 1.722 ± 0.035	0.434HisTrp: 0.434 ± 0.018	0.564HisTyr: 0.564 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.764IleAla: 5.764 ± 0.06	0.432IleCys: 0.432 ± 0.017	2.998IleAsp: 2.998 ± 0.045	3.162IleGlu: 3.162 ± 0.052	1.258IlePhe: 1.258 ± 0.031	4.037IleGly: 4.037 ± 0.056	0.903IleHis: 0.903 ± 0.026	1.417IleIle: 1.417 ± 0.034	1.538IleLys: 1.538 ± 0.039	3.118IleLeu: 3.118 ± 0.049	0.737IleMet: 0.737 ± 0.023	1.483IleAsn: 1.483 ± 0.035	1.977IlePro: 1.977 ± 0.036	1.403IleGln: 1.403 ± 0.029	2.447IleArg: 2.447 ± 0.046	2.329IleSer: 2.329 ± 0.043	2.709IleThr: 2.709 ± 0.043	3.387IleVal: 3.387 ± 0.046	0.517IleTrp: 0.517 ± 0.019	0.941IleTyr: 0.941 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.046LysAla: 4.046 ± 0.071	0.151LysCys: 0.151 ± 0.01	1.641LysAsp: 1.641 ± 0.038	1.393LysGlu: 1.393 ± 0.034	0.842LysPhe: 0.842 ± 0.028	2.451LysGly: 2.451 ± 0.048	0.685LysHis: 0.685 ± 0.021	1.368LysIle: 1.368 ± 0.033	1.345LysLys: 1.345 ± 0.043	3.437LysLeu: 3.437 ± 0.055	0.777LysMet: 0.777 ± 0.022	0.938LysAsn: 0.938 ± 0.03	2.05LysPro: 2.05 ± 0.037	1.131LysGln: 1.131 ± 0.029	2.188LysArg: 2.188 ± 0.04	1.751LysSer: 1.751 ± 0.036	2.036LysThr: 2.036 ± 0.039	2.51LysVal: 2.51 ± 0.045	0.334LysTrp: 0.334 ± 0.015	0.574LysTyr: 0.574 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.419LeuAla: 14.419 ± 0.125	1.026LeuCys: 1.026 ± 0.025	5.893LeuAsp: 5.893 ± 0.065	5.06LeuGlu: 5.06 ± 0.067	3.485LeuPhe: 3.485 ± 0.06	9.145LeuGly: 9.145 ± 0.094	2.418LeuHis: 2.418 ± 0.04	4.541LeuIle: 4.541 ± 0.062	3.808LeuLys: 3.808 ± 0.054	11.448LeuLeu: 11.448 ± 0.133	2.904LeuMet: 2.904 ± 0.043	2.967LeuAsn: 2.967 ± 0.041	6.109LeuPro: 6.109 ± 0.066	4.499LeuGln: 4.499 ± 0.057	7.625LeuArg: 7.625 ± 0.074	6.499LeuSer: 6.499 ± 0.079	5.585LeuThr: 5.585 ± 0.061	8.226LeuVal: 8.226 ± 0.094	1.441LeuTrp: 1.441 ± 0.035	1.931LeuTyr: 1.931 ± 0.039	0.002LeuXaa: 0.002 ± 0.001
Met	3.429MetAla: 3.429 ± 0.051	0.193MetCys: 0.193 ± 0.011	1.269MetAsp: 1.269 ± 0.03	1.145MetGlu: 1.145 ± 0.027	0.725MetPhe: 0.725 ± 0.023	2.223MetGly: 2.223 ± 0.043	0.604MetHis: 0.604 ± 0.021	0.958MetIle: 0.958 ± 0.027	1.123MetLys: 1.123 ± 0.028	2.801MetLeu: 2.801 ± 0.048	0.599MetMet: 0.599 ± 0.023	1.007MetAsn: 1.007 ± 0.024	1.515MetPro: 1.515 ± 0.033	1.126MetGln: 1.126 ± 0.028	1.704MetArg: 1.704 ± 0.035	1.714MetSer: 1.714 ± 0.035	1.609MetThr: 1.609 ± 0.034	2.073MetVal: 2.073 ± 0.036	0.229MetTrp: 0.229 ± 0.013	0.366MetTyr: 0.366 ± 0.015	0.001MetXaa: 0.001 ± 0.001
Asn	3.338AsnAla: 3.338 ± 0.053	0.247AsnCys: 0.247 ± 0.015	1.396AsnAsp: 1.396 ± 0.03	1.306AsnGlu: 1.306 ± 0.028	0.928AsnPhe: 0.928 ± 0.026	2.256AsnGly: 2.256 ± 0.045	0.548AsnHis: 0.548 ± 0.021	1.243AsnIle: 1.243 ± 0.029	0.793AsnLys: 0.793 ± 0.023	2.655AsnLeu: 2.655 ± 0.043	0.619AsnMet: 0.619 ± 0.021	0.794AsnAsn: 0.794 ± 0.023	1.866AsnPro: 1.866 ± 0.036	0.957AsnGln: 0.957 ± 0.028	1.666AsnArg: 1.666 ± 0.033	1.177AsnSer: 1.177 ± 0.03	1.599AsnThr: 1.599 ± 0.036	1.957AsnVal: 1.957 ± 0.036	0.43AsnTrp: 0.43 ± 0.017	0.61AsnTyr: 0.61 ± 0.019	0.001AsnXaa: 0.001 ± 0.001
Pro	6.747ProAla: 6.747 ± 0.086	0.361ProCys: 0.361 ± 0.015	3.137ProAsp: 3.137 ± 0.05	3.259ProGlu: 3.259 ± 0.047	1.831ProPhe: 1.831 ± 0.036	4.706ProGly: 4.706 ± 0.057	1.188ProHis: 1.188 ± 0.028	1.873ProIle: 1.873 ± 0.032	1.442ProLys: 1.442 ± 0.032	5.364ProLeu: 5.364 ± 0.066	1.506ProMet: 1.506 ± 0.031	1.275ProAsn: 1.275 ± 0.031	2.648ProPro: 2.648 ± 0.052	2.136ProGln: 2.136 ± 0.037	2.97ProArg: 2.97 ± 0.046	2.79ProSer: 2.79 ± 0.043	2.859ProThr: 2.859 ± 0.045	4.442ProVal: 4.442 ± 0.056	0.844ProTrp: 0.844 ± 0.027	1.043ProTyr: 1.043 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	5.702GlnAla: 5.702 ± 0.077	0.3GlnCys: 0.3 ± 0.014	1.658GlnAsp: 1.658 ± 0.031	1.587GlnGlu: 1.587 ± 0.033	1.136GlnPhe: 1.136 ± 0.026	3.27GlnGly: 3.27 ± 0.049	1.02GlnHis: 1.02 ± 0.026	1.732GlnIle: 1.732 ± 0.034	1.168GlnLys: 1.168 ± 0.034	4.329GlnLeu: 4.329 ± 0.057	1.054GlnMet: 1.054 ± 0.028	0.886GlnAsn: 0.886 ± 0.024	2.279GlnPro: 2.279 ± 0.04	1.849GlnGln: 1.849 ± 0.04	3.731GlnArg: 3.731 ± 0.055	2.074GlnSer: 2.074 ± 0.038	2.178GlnThr: 2.178 ± 0.034	3.062GlnVal: 3.062 ± 0.048	0.652GlnTrp: 0.652 ± 0.021	0.715GlnTyr: 0.715 ± 0.024	0.001GlnXaa: 0.001 ± 0.001
Arg	7.596ArgAla: 7.596 ± 0.07	0.616ArgCys: 0.616 ± 0.019	3.491ArgAsp: 3.491 ± 0.051	4.267ArgGlu: 4.267 ± 0.05	2.787ArgPhe: 2.787 ± 0.041	4.556ArgGly: 4.556 ± 0.057	1.912ArgHis: 1.912 ± 0.04	3.27ArgIle: 3.27 ± 0.047	1.982ArgLys: 1.982 ± 0.041	7.84ArgLeu: 7.84 ± 0.074	1.915ArgMet: 1.915 ± 0.039	1.699ArgAsn: 1.699 ± 0.033	3.095ArgPro: 3.095 ± 0.045	2.853ArgGln: 2.853 ± 0.052	4.637ArgArg: 4.637 ± 0.062	3.749ArgSer: 3.749 ± 0.055	3.205ArgThr: 3.205 ± 0.049	5.353ArgVal: 5.353 ± 0.064	1.237ArgTrp: 1.237 ± 0.032	1.657ArgTyr: 1.657 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.603SerAla: 6.603 ± 0.075	0.44SerCys: 0.44 ± 0.019	2.66SerAsp: 2.66 ± 0.038	2.659SerGlu: 2.659 ± 0.044	2.06SerPhe: 2.06 ± 0.037	5.263SerGly: 5.263 ± 0.065	1.306SerHis: 1.306 ± 0.03	2.352SerIle: 2.352 ± 0.044	1.525SerLys: 1.525 ± 0.038	6.015SerLeu: 6.015 ± 0.074	1.453SerMet: 1.453 ± 0.032	1.41SerAsn: 1.41 ± 0.032	3.002SerPro: 3.002 ± 0.049	1.925SerGln: 1.925 ± 0.035	3.564SerArg: 3.564 ± 0.046	3.124SerSer: 3.124 ± 0.046	3.12SerThr: 3.12 ± 0.051	4.228SerVal: 4.228 ± 0.054	0.757SerTrp: 0.757 ± 0.023	1.154SerTyr: 1.154 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	6.166ThrAla: 6.166 ± 0.075	0.432ThrCys: 0.432 ± 0.018	2.673ThrAsp: 2.673 ± 0.041	2.659ThrGlu: 2.659 ± 0.036	1.83ThrPhe: 1.83 ± 0.036	4.894ThrGly: 4.894 ± 0.074	1.347ThrHis: 1.347 ± 0.034	2.105ThrIle: 2.105 ± 0.04	1.253ThrLys: 1.253 ± 0.033	6.562ThrLeu: 6.562 ± 0.068	1.198ThrMet: 1.198 ± 0.03	1.262ThrAsn: 1.262 ± 0.034	3.672ThrPro: 3.672 ± 0.053	2.085ThrGln: 2.085 ± 0.041	3.357ThrArg: 3.357 ± 0.055	2.879ThrSer: 2.879 ± 0.044	3.124ThrThr: 3.124 ± 0.048	4.392ThrVal: 4.392 ± 0.056	0.713ThrTrp: 0.713 ± 0.023	1.04ThrTyr: 1.04 ± 0.026	0.001ThrXaa: 0.001 ± 0.001
Val	9.695ValAla: 9.695 ± 0.085	0.823ValCys: 0.823 ± 0.023	4.096ValAsp: 4.096 ± 0.048	4.116ValGlu: 4.116 ± 0.058	2.959ValPhe: 2.959 ± 0.048	5.934ValGly: 5.934 ± 0.076	1.795ValHis: 1.795 ± 0.037	3.594ValIle: 3.594 ± 0.052	2.553ValLys: 2.553 ± 0.05	8.743ValLeu: 8.743 ± 0.089	2.16ValMet: 2.16 ± 0.04	2.286ValAsn: 2.286 ± 0.043	4.122ValPro: 4.122 ± 0.055	3.072ValGln: 3.072 ± 0.048	5.263ValArg: 5.263 ± 0.061	4.397ValSer: 4.397 ± 0.056	4.019ValThr: 4.019 ± 0.051	6.925ValVal: 6.925 ± 0.072	1.148ValTrp: 1.148 ± 0.031	1.505ValTyr: 1.505 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.498TrpAla: 1.498 ± 0.035	0.183TrpCys: 0.183 ± 0.01	0.618TrpAsp: 0.618 ± 0.017	0.55TrpGlu: 0.55 ± 0.02	0.553TrpPhe: 0.553 ± 0.018	1.041TrpGly: 1.041 ± 0.027	0.377TrpHis: 0.377 ± 0.015	0.657TrpIle: 0.657 ± 0.025	0.43TrpLys: 0.43 ± 0.018	2.171TrpLeu: 2.171 ± 0.048	0.5TrpMet: 0.5 ± 0.019	0.434TrpAsn: 0.434 ± 0.017	0.802TrpPro: 0.802 ± 0.026	0.741TrpGln: 0.741 ± 0.023	1.262TrpArg: 1.262 ± 0.034	0.824TrpSer: 0.824 ± 0.019	0.772TrpThr: 0.772 ± 0.023	1.135TrpVal: 1.135 ± 0.03	0.303TrpTrp: 0.303 ± 0.013	0.284TrpTyr: 0.284 ± 0.015	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.4TyrAla: 2.4 ± 0.04	0.225TyrCys: 0.225 ± 0.013	1.044TyrAsp: 1.044 ± 0.028	1.075TyrGlu: 1.075 ± 0.029	0.847TyrPhe: 0.847 ± 0.024	1.886TyrGly: 1.886 ± 0.041	0.418TyrHis: 0.418 ± 0.015	0.758TyrIle: 0.758 ± 0.023	0.618TyrLys: 0.618 ± 0.022	2.245TyrLeu: 2.245 ± 0.039	0.421TyrMet: 0.421 ± 0.018	0.562TyrAsn: 0.562 ± 0.02	1.058TyrPro: 1.058 ± 0.029	0.792TyrGln: 0.792 ± 0.021	1.537TyrArg: 1.537 ± 0.035	1.075TyrSer: 1.075 ± 0.025	1.151TyrThr: 1.151 ± 0.025	1.52TyrVal: 1.52 ± 0.03	0.359TyrTrp: 0.359 ± 0.017	0.495TyrTyr: 0.495 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4859 proteins (1561390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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