Ancylobacter rudongensis
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4123 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G4QSE3|A0A1G4QSE3_9RHIZ Signal peptidase I OS=Ancylobacter rudongensis OX=177413 GN=SAMN05660859_1389 PE=3 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 YY3 pKa = 9.69 AVLSVAALLVAGLSPAGAQQPIEE26 pKa = 4.04 VHH28 pKa = 5.87 PNKK31 pKa = 9.87 PAGGGLEE38 pKa = 3.91 ISIPPINNPNYY49 pKa = 10.27 LDD51 pKa = 4.04 YY52 pKa = 11.48 GPLPDD57 pKa = 5.55 KK58 pKa = 10.97 PGADD62 pKa = 3.19 KK63 pKa = 11.31 SQDD66 pKa = 3.4 YY67 pKa = 9.95 MEE69 pKa = 4.36 QAGGNYY75 pKa = 9.26 EE76 pKa = 4.1 NLSGPGSDD84 pKa = 3.16 VDD86 pKa = 4.07 ADD88 pKa = 3.84 VMPDD92 pKa = 2.89 SDD94 pKa = 4.04 GAYY97 pKa = 9.13 EE98 pKa = 4.35 SPLDD102 pKa = 3.63
Molecular weight: 10.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A1G4RIU1|A0A1G4RIU1_9RHIZ Ribosomal RNA small subunit methyltransferase E OS=Ancylobacter rudongensis OX=177413 GN=SAMN05660859_1690 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 TGRR28 pKa = 11.84 KK29 pKa = 8.9 IINLRR34 pKa = 11.84 RR35 pKa = 11.84 AHH37 pKa = 5.13 GRR39 pKa = 11.84 KK40 pKa = 9.31 RR41 pKa = 11.84 LSAA44 pKa = 4.01
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4123
0
4123
1337184
25
2981
324.3
34.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.791 ± 0.058
0.776 ± 0.012
5.403 ± 0.034
5.656 ± 0.03
3.685 ± 0.024
8.977 ± 0.041
1.954 ± 0.018
4.757 ± 0.029
2.755 ± 0.032
10.618 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.017
2.269 ± 0.022
5.681 ± 0.037
2.801 ± 0.023
7.274 ± 0.041
4.98 ± 0.028
5.254 ± 0.03
7.658 ± 0.031
1.291 ± 0.015
2.1 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here