Amino acid dipepetide frequency for Ancylobacter rudongensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.186AlaAla: 20.186 ± 0.189	1.044AlaCys: 1.044 ± 0.028	6.847AlaAsp: 6.847 ± 0.086	7.766AlaGlu: 7.766 ± 0.091	4.963AlaPhe: 4.963 ± 0.067	12.642AlaGly: 12.642 ± 0.117	2.472AlaHis: 2.472 ± 0.051	6.031AlaIle: 6.031 ± 0.076	3.64AlaLys: 3.64 ± 0.068	16.114AlaLeu: 16.114 ± 0.156	3.472AlaMet: 3.472 ± 0.05	2.76AlaAsn: 2.76 ± 0.052	7.674AlaPro: 7.674 ± 0.118	4.406AlaGln: 4.406 ± 0.062	10.795AlaArg: 10.795 ± 0.132	6.749AlaSer: 6.749 ± 0.072	6.639AlaThr: 6.639 ± 0.078	9.522AlaVal: 9.522 ± 0.105	1.502AlaTrp: 1.502 ± 0.035	2.688AlaTyr: 2.688 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.027CysAla: 1.027 ± 0.034	0.096CysCys: 0.096 ± 0.009	0.447CysAsp: 0.447 ± 0.017	0.42CysGlu: 0.42 ± 0.019	0.307CysPhe: 0.307 ± 0.015	0.899CysGly: 0.899 ± 0.026	0.203CysHis: 0.203 ± 0.013	0.335CysIle: 0.335 ± 0.017	0.153CysLys: 0.153 ± 0.01	0.791CysLeu: 0.791 ± 0.029	0.13CysMet: 0.13 ± 0.011	0.16CysAsn: 0.16 ± 0.011	0.42CysPro: 0.42 ± 0.018	0.186CysGln: 0.186 ± 0.012	0.583CysArg: 0.583 ± 0.023	0.34CysSer: 0.34 ± 0.016	0.399CysThr: 0.399 ± 0.018	0.582CysVal: 0.582 ± 0.022	0.099CysTrp: 0.099 ± 0.009	0.178CysTyr: 0.178 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.965AspAla: 6.965 ± 0.075	0.42AspCys: 0.42 ± 0.017	2.821AspAsp: 2.821 ± 0.068	3.374AspGlu: 3.374 ± 0.054	2.102AspPhe: 2.102 ± 0.04	5.177AspGly: 5.177 ± 0.083	1.122AspHis: 1.122 ± 0.03	2.881AspIle: 2.881 ± 0.05	1.467AspLys: 1.467 ± 0.038	5.67AspLeu: 5.67 ± 0.069	1.256AspMet: 1.256 ± 0.028	1.066AspAsn: 1.066 ± 0.032	3.587AspPro: 3.587 ± 0.059	1.383AspGln: 1.383 ± 0.032	3.782AspArg: 3.782 ± 0.06	1.984AspSer: 1.984 ± 0.039	2.548AspThr: 2.548 ± 0.052	4.081AspVal: 4.081 ± 0.056	0.899AspTrp: 0.899 ± 0.026	1.446AspTyr: 1.446 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	8.665GluAla: 8.665 ± 0.113	0.331GluCys: 0.331 ± 0.016	2.604GluAsp: 2.604 ± 0.052	3.202GluGlu: 3.202 ± 0.068	1.538GluPhe: 1.538 ± 0.038	4.973GluGly: 4.973 ± 0.069	1.075GluHis: 1.075 ± 0.033	3.262GluIle: 3.262 ± 0.05	1.859GluLys: 1.859 ± 0.046	5.388GluLeu: 5.388 ± 0.064	1.447GluMet: 1.447 ± 0.037	1.375GluAsn: 1.375 ± 0.031	2.927GluPro: 2.927 ± 0.05	1.778GluGln: 1.778 ± 0.04	5.12GluArg: 5.12 ± 0.085	2.221GluSer: 2.221 ± 0.044	3.178GluThr: 3.178 ± 0.053	4.128GluVal: 4.128 ± 0.057	0.666GluTrp: 0.666 ± 0.023	0.82GluTyr: 0.82 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.951PheAla: 4.951 ± 0.061	0.358PheCys: 0.358 ± 0.016	2.511PheAsp: 2.511 ± 0.044	2.029PheGlu: 2.029 ± 0.039	1.422PhePhe: 1.422 ± 0.037	3.848PheGly: 3.848 ± 0.067	0.749PheHis: 0.749 ± 0.025	1.699PheIle: 1.699 ± 0.035	0.885PheLys: 0.885 ± 0.028	3.546PheLeu: 3.546 ± 0.054	0.756PheMet: 0.756 ± 0.023	0.937PheAsn: 0.937 ± 0.026	1.664PhePro: 1.664 ± 0.037	0.927PheGln: 0.927 ± 0.026	2.241PheArg: 2.241 ± 0.045	2.006PheSer: 2.006 ± 0.04	2.116PheThr: 2.116 ± 0.042	2.858PheVal: 2.858 ± 0.051	0.528PheTrp: 0.528 ± 0.021	0.82PheTyr: 0.82 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	11.038GlyAla: 11.038 ± 0.122	0.786GlyCys: 0.786 ± 0.027	4.281GlyAsp: 4.281 ± 0.066	5.264GlyGlu: 5.264 ± 0.065	3.812GlyPhe: 3.812 ± 0.056	7.908GlyGly: 7.908 ± 0.124	1.97GlyHis: 1.97 ± 0.042	4.391GlyIle: 4.391 ± 0.062	2.786GlyLys: 2.786 ± 0.045	9.773GlyLeu: 9.773 ± 0.105	2.315GlyMet: 2.315 ± 0.047	1.929GlyAsn: 1.929 ± 0.043	4.134GlyPro: 4.134 ± 0.066	2.711GlyGln: 2.711 ± 0.059	6.712GlyArg: 6.712 ± 0.087	4.474GlySer: 4.474 ± 0.081	5.151GlyThr: 5.151 ± 0.1	6.69GlyVal: 6.69 ± 0.08	1.49GlyTrp: 1.49 ± 0.037	2.155GlyTyr: 2.155 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.527HisAla: 2.527 ± 0.048	0.192HisCys: 0.192 ± 0.012	1.143HisAsp: 1.143 ± 0.031	1.048HisGlu: 1.048 ± 0.031	0.79HisPhe: 0.79 ± 0.025	1.917HisGly: 1.917 ± 0.036	0.526HisHis: 0.526 ± 0.024	0.881HisIle: 0.881 ± 0.025	0.385HisLys: 0.385 ± 0.017	2.021HisLeu: 2.021 ± 0.042	0.506HisMet: 0.506 ± 0.02	0.382HisAsn: 0.382 ± 0.018	1.317HisPro: 1.317 ± 0.038	0.5HisGln: 0.5 ± 0.022	1.384HisArg: 1.384 ± 0.034	0.842HisSer: 0.842 ± 0.029	0.78HisThr: 0.78 ± 0.024	1.547HisVal: 1.547 ± 0.036	0.31HisTrp: 0.31 ± 0.018	0.544HisTyr: 0.544 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.289IleAla: 7.289 ± 0.084	0.463IleCys: 0.463 ± 0.019	3.403IleAsp: 3.403 ± 0.06	3.4IleGlu: 3.4 ± 0.048	1.481IlePhe: 1.481 ± 0.04	5.126IleGly: 5.126 ± 0.072	0.815IleHis: 0.815 ± 0.026	1.93IleIle: 1.93 ± 0.042	1.168IleLys: 1.168 ± 0.036	4.053IleLeu: 4.053 ± 0.058	0.81IleMet: 0.81 ± 0.024	1.243IleAsn: 1.243 ± 0.032	2.158IlePro: 2.158 ± 0.041	1.007IleGln: 1.007 ± 0.028	2.85IleArg: 2.85 ± 0.051	2.331IleSer: 2.331 ± 0.044	2.449IleThr: 2.449 ± 0.039	4.092IleVal: 4.092 ± 0.06	0.501IleTrp: 0.501 ± 0.02	1.0IleTyr: 1.0 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.78LysAla: 3.78 ± 0.074	0.126LysCys: 0.126 ± 0.009	1.416LysAsp: 1.416 ± 0.037	1.398LysGlu: 1.398 ± 0.033	0.698LysPhe: 0.698 ± 0.022	2.374LysGly: 2.374 ± 0.042	0.43LysHis: 0.43 ± 0.018	1.401LysIle: 1.401 ± 0.034	1.036LysLys: 1.036 ± 0.037	2.862LysLeu: 2.862 ± 0.054	0.656LysMet: 0.656 ± 0.026	0.684LysAsn: 0.684 ± 0.023	1.827LysPro: 1.827 ± 0.046	0.798LysGln: 0.798 ± 0.028	1.927LysArg: 1.927 ± 0.04	1.422LysSer: 1.422 ± 0.037	1.544LysThr: 1.544 ± 0.034	2.354LysVal: 2.354 ± 0.047	0.324LysTrp: 0.324 ± 0.016	0.49LysTyr: 0.49 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	16.745LeuAla: 16.745 ± 0.169	0.861LeuCys: 0.861 ± 0.027	6.408LeuAsp: 6.408 ± 0.082	5.075LeuGlu: 5.075 ± 0.066	3.807LeuPhe: 3.807 ± 0.056	9.035LeuGly: 9.035 ± 0.088	1.795LeuHis: 1.795 ± 0.04	4.961LeuIle: 4.961 ± 0.074	3.28LeuLys: 3.28 ± 0.059	10.217LeuLeu: 10.217 ± 0.133	2.366LeuMet: 2.366 ± 0.044	2.308LeuAsn: 2.308 ± 0.045	6.23LeuPro: 6.23 ± 0.078	2.389LeuGln: 2.389 ± 0.044	7.084LeuArg: 7.084 ± 0.075	6.114LeuSer: 6.114 ± 0.076	5.721LeuThr: 5.721 ± 0.069	8.504LeuVal: 8.504 ± 0.093	1.211LeuTrp: 1.211 ± 0.032	2.067LeuTyr: 2.067 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	3.075MetAla: 3.075 ± 0.044	0.15MetCys: 0.15 ± 0.01	1.027MetAsp: 1.027 ± 0.029	1.143MetGlu: 1.143 ± 0.029	0.645MetPhe: 0.645 ± 0.02	1.726MetGly: 1.726 ± 0.035	0.377MetHis: 0.377 ± 0.017	1.14MetIle: 1.14 ± 0.027	0.835MetLys: 0.835 ± 0.026	2.419MetLeu: 2.419 ± 0.046	0.6MetMet: 0.6 ± 0.025	0.728MetAsn: 0.728 ± 0.026	1.484MetPro: 1.484 ± 0.032	0.667MetGln: 0.667 ± 0.025	1.765MetArg: 1.765 ± 0.04	1.606MetSer: 1.606 ± 0.033	1.625MetThr: 1.625 ± 0.032	1.7MetVal: 1.7 ± 0.042	0.213MetTrp: 0.213 ± 0.012	0.278MetTyr: 0.278 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.942AsnAla: 2.942 ± 0.05	0.195AsnCys: 0.195 ± 0.012	1.121AsnAsp: 1.121 ± 0.033	1.119AsnGlu: 1.119 ± 0.029	0.787AsnPhe: 0.787 ± 0.026	2.201AsnGly: 2.201 ± 0.051	0.452AsnHis: 0.452 ± 0.02	1.179AsnIle: 1.179 ± 0.031	0.567AsnLys: 0.567 ± 0.022	2.316AsnLeu: 2.316 ± 0.053	0.515AsnMet: 0.515 ± 0.021	0.563AsnAsn: 0.563 ± 0.025	1.65AsnPro: 1.65 ± 0.039	0.59AsnGln: 0.59 ± 0.022	1.608AsnArg: 1.608 ± 0.035	0.915AsnSer: 0.915 ± 0.028	1.084AsnThr: 1.084 ± 0.029	1.867AsnVal: 1.867 ± 0.039	0.403AsnTrp: 0.403 ± 0.018	0.617AsnTyr: 0.617 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	8.386ProAla: 8.386 ± 0.119	0.309ProCys: 0.309 ± 0.017	3.644ProAsp: 3.644 ± 0.062	3.933ProGlu: 3.933 ± 0.059	2.176ProPhe: 2.176 ± 0.048	5.29ProGly: 5.29 ± 0.079	1.139ProHis: 1.139 ± 0.034	2.19ProIle: 2.19 ± 0.046	1.422ProLys: 1.422 ± 0.036	5.472ProLeu: 5.472 ± 0.068	1.228ProMet: 1.228 ± 0.031	1.279ProAsn: 1.279 ± 0.03	3.315ProPro: 3.315 ± 0.078	1.554ProGln: 1.554 ± 0.036	3.405ProArg: 3.405 ± 0.058	2.929ProSer: 2.929 ± 0.051	2.75ProThr: 2.75 ± 0.045	4.562ProVal: 4.562 ± 0.06	0.69ProTrp: 0.69 ± 0.025	1.137ProTyr: 1.137 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.029GlnAla: 4.029 ± 0.055	0.178GlnCys: 0.178 ± 0.013	1.259GlnAsp: 1.259 ± 0.031	1.395GlnGlu: 1.395 ± 0.035	0.928GlnPhe: 0.928 ± 0.026	2.31GlnGly: 2.31 ± 0.047	0.552GlnHis: 0.552 ± 0.022	1.564GlnIle: 1.564 ± 0.032	0.879GlnLys: 0.879 ± 0.026	2.743GlnLeu: 2.743 ± 0.05	0.83GlnMet: 0.83 ± 0.03	0.71GlnAsn: 0.71 ± 0.028	1.589GlnPro: 1.589 ± 0.038	1.047GlnGln: 1.047 ± 0.041	2.208GlnArg: 2.208 ± 0.045	1.425GlnSer: 1.425 ± 0.038	1.45GlnThr: 1.45 ± 0.037	2.066GlnVal: 2.066 ± 0.041	0.351GlnTrp: 0.351 ± 0.016	0.491GlnTyr: 0.491 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.6ArgAla: 9.6 ± 0.115	0.498ArgCys: 0.498 ± 0.02	3.769ArgAsp: 3.769 ± 0.057	4.363ArgGlu: 4.363 ± 0.065	3.019ArgPhe: 3.019 ± 0.054	5.147ArgGly: 5.147 ± 0.067	1.746ArgHis: 1.746 ± 0.044	3.707ArgIle: 3.707 ± 0.057	1.674ArgLys: 1.674 ± 0.041	8.866ArgLeu: 8.866 ± 0.099	1.696ArgMet: 1.696 ± 0.035	1.567ArgAsn: 1.567 ± 0.036	4.181ArgPro: 4.181 ± 0.079	2.311ArgGln: 2.311 ± 0.046	6.304ArgArg: 6.304 ± 0.086	3.306ArgSer: 3.306 ± 0.054	3.421ArgThr: 3.421 ± 0.053	5.077ArgVal: 5.077 ± 0.069	0.945ArgTrp: 0.945 ± 0.027	1.547ArgTyr: 1.547 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.194SerAla: 6.194 ± 0.073	0.387SerCys: 0.387 ± 0.019	2.494SerAsp: 2.494 ± 0.042	2.516SerGlu: 2.516 ± 0.044	2.26SerPhe: 2.26 ± 0.039	5.307SerGly: 5.307 ± 0.07	1.005SerHis: 1.005 ± 0.029	2.302SerIle: 2.302 ± 0.047	1.257SerLys: 1.257 ± 0.034	5.32SerLeu: 5.32 ± 0.064	1.123SerMet: 1.123 ± 0.028	1.154SerAsn: 1.154 ± 0.034	2.886SerPro: 2.886 ± 0.045	1.305SerGln: 1.305 ± 0.035	3.397SerArg: 3.397 ± 0.057	2.551SerSer: 2.551 ± 0.055	2.57SerThr: 2.57 ± 0.049	3.835SerVal: 3.835 ± 0.059	0.761SerTrp: 0.761 ± 0.027	1.176SerTyr: 1.176 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.378ThrAla: 6.378 ± 0.065	0.387ThrCys: 0.387 ± 0.018	2.646ThrAsp: 2.646 ± 0.052	2.493ThrGlu: 2.493 ± 0.043	1.959ThrPhe: 1.959 ± 0.036	5.023ThrGly: 5.023 ± 0.073	1.037ThrHis: 1.037 ± 0.031	2.536ThrIle: 2.536 ± 0.046	1.206ThrLys: 1.206 ± 0.032	6.648ThrLeu: 6.648 ± 0.085	1.016ThrMet: 1.016 ± 0.03	1.162ThrAsn: 1.162 ± 0.03	3.61ThrPro: 3.61 ± 0.057	1.418ThrGln: 1.418 ± 0.042	3.475ThrArg: 3.475 ± 0.054	2.729ThrSer: 2.729 ± 0.049	2.763ThrThr: 2.763 ± 0.05	4.3ThrVal: 4.3 ± 0.072	0.552ThrTrp: 0.552 ± 0.022	1.209ThrTyr: 1.209 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.311ValAla: 10.311 ± 0.103	0.618ValCys: 0.618 ± 0.025	4.216ValAsp: 4.216 ± 0.055	4.823ValGlu: 4.823 ± 0.062	2.828ValPhe: 2.828 ± 0.048	5.889ValGly: 5.889 ± 0.088	1.344ValHis: 1.344 ± 0.034	3.79ValIle: 3.79 ± 0.054	2.09ValLys: 2.09 ± 0.048	8.184ValLeu: 8.184 ± 0.089	1.799ValMet: 1.799 ± 0.038	1.782ValAsn: 1.782 ± 0.044	4.413ValPro: 4.413 ± 0.054	1.866ValGln: 1.866 ± 0.036	5.266ValArg: 5.266 ± 0.066	4.052ValSer: 4.052 ± 0.063	4.61ValThr: 4.61 ± 0.066	6.327ValVal: 6.327 ± 0.091	0.891ValTrp: 0.891 ± 0.03	1.481ValTyr: 1.481 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.232TrpAla: 1.232 ± 0.032	0.143TrpCys: 0.143 ± 0.011	0.601TrpAsp: 0.601 ± 0.023	0.591TrpGlu: 0.591 ± 0.023	0.49TrpPhe: 0.49 ± 0.019	0.927TrpGly: 0.927 ± 0.032	0.322TrpHis: 0.322 ± 0.016	0.574TrpIle: 0.574 ± 0.022	0.416TrpLys: 0.416 ± 0.016	1.633TrpLeu: 1.633 ± 0.044	0.313TrpMet: 0.313 ± 0.017	0.417TrpAsn: 0.417 ± 0.017	0.708TrpPro: 0.708 ± 0.027	0.532TrpGln: 0.532 ± 0.022	1.209TrpArg: 1.209 ± 0.032	0.764TrpSer: 0.764 ± 0.027	0.709TrpThr: 0.709 ± 0.024	0.808TrpVal: 0.808 ± 0.027	0.242TrpTrp: 0.242 ± 0.015	0.281TrpTyr: 0.281 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.591TyrAla: 2.591 ± 0.045	0.215TyrCys: 0.215 ± 0.013	1.37TyrAsp: 1.37 ± 0.038	1.206TyrGlu: 1.206 ± 0.03	0.836TyrPhe: 0.836 ± 0.025	2.049TyrGly: 2.049 ± 0.042	0.415TyrHis: 0.415 ± 0.016	0.815TyrIle: 0.815 ± 0.025	0.541TyrLys: 0.541 ± 0.02	2.142TyrLeu: 2.142 ± 0.044	0.368TyrMet: 0.368 ± 0.017	0.486TyrAsn: 0.486 ± 0.023	1.034TyrPro: 1.034 ± 0.028	0.632TyrGln: 0.632 ± 0.024	1.63TyrArg: 1.63 ± 0.037	1.039TyrSer: 1.039 ± 0.031	1.04TyrThr: 1.04 ± 0.032	1.679TyrVal: 1.679 ± 0.037	0.335TyrTrp: 0.335 ± 0.016	0.581TyrTyr: 0.581 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4123 proteins (1337184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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