Ulvibacter sp. MAR_2010_11
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2733 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N0EWT1|A0A2N0EWT1_9FLAO Type I restriction and modification enzyme subunit R-like protein OS=Ulvibacter sp. MAR_2010_11 OX=1250229 GN=ATE92_2754 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.53 NVVQILAVALISATAFSQTARR23 pKa = 11.84 VNVIHH28 pKa = 6.73 NSADD32 pKa = 3.24 AAAEE36 pKa = 4.02 QVDD39 pKa = 4.72 VYY41 pKa = 11.36 LDD43 pKa = 3.33 AALALDD49 pKa = 3.61 NFTFRR54 pKa = 11.84 TEE56 pKa = 3.76 SGFIDD61 pKa = 4.7 FPAEE65 pKa = 3.8 VEE67 pKa = 4.48 VIIGVAPPNSTSSGDD82 pKa = 3.85 AIASFPVTLMDD93 pKa = 3.78 GEE95 pKa = 4.69 TYY97 pKa = 10.38 IVVADD102 pKa = 5.09 GIVSPTGYY110 pKa = 10.92 DD111 pKa = 3.53 PIQPFSLEE119 pKa = 3.97 VYY121 pKa = 10.86 AMGRR125 pKa = 11.84 EE126 pKa = 4.05 TAAVATNTDD135 pKa = 3.5 VLVHH139 pKa = 6.6 HH140 pKa = 7.28 GSTDD144 pKa = 3.2 APTVDD149 pKa = 3.84 VVEE152 pKa = 4.86 TGAGAGTIVDD162 pKa = 4.23 NITYY166 pKa = 10.19 SEE168 pKa = 4.08 FQGYY172 pKa = 10.11 LEE174 pKa = 5.69 LPTADD179 pKa = 3.21 YY180 pKa = 10.88 VLEE183 pKa = 4.19 VRR185 pKa = 11.84 DD186 pKa = 3.57 EE187 pKa = 4.25 TGTVGVAAYY196 pKa = 7.43 EE197 pKa = 4.34 APLATLNLDD206 pKa = 3.63 GAALVVLASGFLNPANNSNGPAFGLWVSTGAPGALLEE243 pKa = 4.87 LPSAALGVNDD253 pKa = 4.84 FDD255 pKa = 3.6 ITSFALYY262 pKa = 9.23 PNPTTEE268 pKa = 3.94 QLNVSVSGIEE278 pKa = 3.66 LANYY282 pKa = 8.64 NVSITDD288 pKa = 3.38 MLGRR292 pKa = 11.84 TVLSNLTVTDD302 pKa = 3.47 NSVDD306 pKa = 3.44 VTALHH311 pKa = 6.29 GGIYY315 pKa = 9.61 QLSIMDD321 pKa = 4.06 GTTTIATKK329 pKa = 10.72 KK330 pKa = 10.32 FIKK333 pKa = 10.1 QQ334 pKa = 3.07
Molecular weight: 34.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.579
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 1.125
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A2N0ERL1|A0A2N0ERL1_9FLAO LuxR family two component transcriptional regulator OS=Ulvibacter sp. MAR_2010_11 OX=1250229 GN=ATE92_0867 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.5 KK27 pKa = 10.78 KK28 pKa = 9.37 KK29 pKa = 10.83
Molecular weight: 3.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2733
0
2733
915841
29
4170
335.1
37.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.936 ± 0.044
0.766 ± 0.018
5.521 ± 0.046
6.674 ± 0.052
5.175 ± 0.033
6.74 ± 0.057
1.751 ± 0.022
7.655 ± 0.04
7.097 ± 0.079
9.245 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.023
5.747 ± 0.04
3.557 ± 0.035
3.336 ± 0.026
3.505 ± 0.039
6.445 ± 0.03
6.133 ± 0.066
6.532 ± 0.044
1.019 ± 0.017
3.945 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here